This volume provides a collection of methodologies for basic
research, clinical diagnosis, and treatment pertaining to food
allergens, including food allergen production, purification,
characterization, detection, quantification, and bioinformatics
approaches to modern food allergen studies. The chapters in the
book are divided into 4 parts: Part I discusses food allergen
purification and production, and explores methods of producing
recombinant food allergens in bacterial and yeast expression
systems; Part II looks at allergen discovery, detection, and
quantification covering 3 types of methods-DNA-, protein-, and
cell-based methods; Part III focuses on allergenic epitope mapping;
and Part IV talks about future developments concentrated around new
concepts of allergenicity as an outcome of protein and food matrix
interactions. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
comprehensive, Food Allergens: Methods and Protocols is a valuable
resource for immunologists, biochemists, molecular biologists, and
medical doctors and students working in the food allergy field.
This book is also useful for people in the food industry,
legislators, food standard agencies, allergologists, pediatricians,
and clinicians in the allergic diseases and immunology fields.
General
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