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High-Performance Gradient Elution - The Practical Application of the Linear-Solvent-Strength Model (Hardcover)
Loot Price: R4,265
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High-Performance Gradient Elution - The Practical Application of the Linear-Solvent-Strength Model (Hardcover)
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Gradient elution demystified
Of the various ways in which chromatography is applied today, few
have been as misunderstood as the technique of gradient elution,
which presents many challenges compared to isocratic separation.
When properly explained, however, gradient elution can be less
difficult to understand and much easier to use than often assumed.
Written by two well-known authorities in liquid chromatography,
High-Performance Gradient Elution: The Practical Application of the
Linear-Solvent-Strength Model takes the mystery out of the practice
of gradient elution and helps remove barriers to the practical
application of this important separation technique. The book
presents a systematic approach to the current understanding of
gradient elution, describing theory, methodology, and applications
across many of the fields that use liquid chromatography as a
primary analytical tool.
This up-to-date, practical, and comprehensive treatment of gradient
elution:
* Provides specific, step-by-step recommendations for developing a
gradient separation for any sample
* Describes the best approach for troubleshooting problems with
gradient methods
* Guides the reader on the equipment used for gradient
elution
* Lists which conditions should be varied first during method
development, and explains how to interpret scouting gradients
* Explains how to avoid problems in transferring gradient methods
With a focus on the use of linear solvent strength (LSS) theory for
predicting gradient LC behavior and separations by reversed-phase
HPLC, High-Performance Gradient Elution gives every chromatographer
access to this useful tool.
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