The general picture we have of membranes and of the properties of
the proteins imbedded in them, has, arguably emerged directly from
probe studies over the past 20 years. This work is designed to make
these techniques accessible to a board audience of cell biologists.
The techniques discussed revolve primarily around the fluorescence
of membrane probes, but applications of light absorption and Raman
scattering are included. In addition to reviews of the major
applications, most chapters include information on the required
apparatus, experimental design, data analysis, and potential pit
falls.
General
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