Books > Science & Mathematics > Chemistry > Analytical chemistry > Qualitative analytical chemistry > Chromatography
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Chromatography - Concepts, Methods and Applications (Hardcover)
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Chromatography - Concepts, Methods and Applications (Hardcover)
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Chromatography has become a cornerstone of separation science, that
branch of chemistry devoted to separating compounds from mixtures.
Chromatography is one of several separation techniques defined as
differential migration from a narrow initial zone. Electrophoresis
is another member of this group. In this case, the driving force is
an electric field, which exerts different forces on solutes of
different ionic charge. The resistive force is the viscosity of the
nonflowing solvent. The combination of these forces yields ion
mobilities peculiar to each solute. There are two main categories
of chromatography: preparative and analytical. Analytical work uses
small sample sizes; the objective is to separate compounds in order
to identify them. Preparative work uses large quantities of samples
and collects the output in bulk; the point of the chromatography
here is to remove impurities from a commercial product. The two are
not mutually exclusive. Chromatography has numerous applications in
biological and chemical fields. It is widely used in biochemical
research for the separation and identification of chemical
compounds of biological origin. In the petroleum industry the
technique is employed to analyze complex mixtures of hydrocarbons.
Pharmaceutical industries use this method both to prepare huge
quantities of extremely pure materials, and also to analyze the
purified compounds for trace contaminants. These separation
techniques like chromatography gain importance in different kinds
of companies, different departments like Fuel Industry,
biotechnology, biochemical processes, and forensic science.
Chromatography is used for quality analyses and checker in the food
industry, by identifying and separating, analyzing additives,
vitamins, preservatives, proteins, and amino acids. Chromatography
like HPLC is used in DNA fingerprinting and bioinformatics.
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