Deficiencies in sperm function are usually the result of
spermatogenic defects. Spermatogenesis is a biologically complex
and essential process during which spermatogonia undergo meiotic
recombination, reduction of the genome to a haploid state, and
extensive cellular modifications that result in a motile cell
capable of traversing the female reproductive tract, withstanding
various potential assaults to viability, and finally successfully
fertilizing a mature oocyte to give rise to an embryo. Defects in
any step of spermatogenesis or spermatogenesis can lead to male
infertility, a disease that affects approximately 5-7% of the
population."Spermiogenesis and Spermatogenesis: Methods and
Protocols"details protocols used in the study of spermatogenesis,
clinical analytical protocols, and basic techniques used in
clinical andrology laboratories, such as obtaining accurate results
for a sperm count, and advanced procedures, such as genome-wide
genetic study tools and evaluation of nuclear proteins.Written in
the successful"Methods in Molecular Biology "series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible protocols, and notes on troubleshooting and avoiding
known pitfalls.
Authoritative and easily accessible, "Spermiogenesis and
Spermatogenesis: Methods and Protocols"isunique in its breadth, and
will be a useful reference for clinicians and researchers
alike."
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