Few problems in protein biochemistry have proven to be as
challenging and recalcitrant as the molecular description of
nitrogenase, the catalyst of one of the most remarkable chemical
transformations in biological systems: the nucleotide-dependent
reduction of atmospheric dinitrogen to bioavailable ammonia. In
"Nitrogen Fixation: Methods and Protocols," recognized experts in
the field provide an up-to-date, in-depth overview of the methods
that have been applied to studying the nitrogenase at a molecular
level, ranging from genetic, biochemical, spectroscopic, and
chemical methods to theoretical calculations. In addition,
techniques used to study an enzyme system that is homologous to
nitrogenase are described in this book. Written in the highly
successful "Methods in Molecular Biology " series format, methods
chapters include introductions to their respective chapters, lists
of the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Practical and cutting-edge, "Nitrogen Fixation: Methods and
Protocols" will be useful for anyone interested in nitrogenase
research and willing to venture further toward addressing the
remaining mechanistic and biosynthetic questions of this
fascinating enzyme system."
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