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The Wilms' Tumor (WT1) Gene - Methods and Protocols (Hardcover, 1st ed. 2016)
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The Wilms' Tumor (WT1) Gene - Methods and Protocols (Hardcover, 1st ed. 2016)
Series: Methods in Molecular Biology, 1467
Expected to ship within 12 - 17 working days
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This volume provides a thorough overview of the Wilms' Tumour Gene
(WT1). The book begins with three review chapters that cover the
involvement of WT1 in pediatric cancer, kidney disease, and tissue
development and homeostasis. The next few chapters discuss cell
marking and lineage tracing, epicardial cell methodology, colony
forming assays for bone marrow stem cells, angiogenesis assays and
zebrafish tools. The next group of chapters explores the latest
tools in genomics, molecular biology, and biochemistry. They
discuss dissecting transcription factor function in cell free
systems, ChiP seq, proteomics, RNA interactome, and multiphoton
imaging of lipids, measuring the binding constants of
protein-nucleic acid interactions, and bioinformatics approaches
for analyzing Next Generation Sequence data. The final chapter
discusses protocols for clinical trials for immune therapy using
anti-WT1 peptides. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls. Practical
and thorough, The Wilms' Tumour (WT1) Gene: Methods and Protocols
is a valuable resource for anyone who is interested in the diverse
methodologies used in WT1 research.
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