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Studies on Amylase Produced by Some Actinomycetes (Paperback)
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Studies on Amylase Produced by Some Actinomycetes (Paperback)
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This book includes taxonomical, physiological, biochemical and
molecular studies of amylolytic thermophilic actinomycetes. A total
of 36 actinomycetes were isolated from soil, mushroom compost and
other compost samples. Four of the isolated thermophilic
actinomycetes that exhibited the highest amylolytic activities were
characterized and identified as: Thermoactinomyces sp, T. vulgaris
and T. intermedius (two isolates). T. vulgaris, the best producer
of -amylase, was selected for further investigation. The optimum
conditions for the production are: 24 hours incubation, 55 C, pH
7.0, starch and tryptone as carbon and nitrogen sources,
respectively. At different conditions of production, -amylase
electrophoresis profile had the same pattern of two isozyme with
molecular weight between 135 and 145 kDa. Purification of this
-amylase was carried out by ammonium sulfate precipitation,
dialysis, gel filtration and anion exchange chromatography.
Physiochemical and molecular studies of the crude and purified
enzyme revealed a novel thermostable, high molecular weight,
calcium independent with wide pH range alpha-amylase. This enzyme
could be of importance for industrial applications.
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