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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
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Proteomic Profiling and Analytical Chemistry - The Crossroads (Paperback, 2nd edition)
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Proteomic Profiling and Analytical Chemistry - The Crossroads (Paperback, 2nd edition)
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Proteomic Profiling and Analytical Chemistry: The Crossroads,
Second Edition helps scientists without a strong background in
analytical chemistry to understand principles of the multistep
proteomic experiment necessary for its successful completion. It
also helps researchers who do have an analytical chemistry
background to break into the proteomics field. Highlighting points
of junction between proteomics and analytical chemistry, this
resource links experimental design with analytical measurements,
data analysis, and quality control. This targeted point of view
will help both biologists and chemists to better understand all
components of a complex proteomic study. The book provides detailed
coverage of experimental aspects such as sample preparation,
protein extraction and precipitation, gel electrophoresis,
microarrays, dynamics of fluorescent dyes, and more. The key
feature of this book is a direct link between multistep proteomic
strategy and quality control routinely applied in analytical
chemistry. This second edition features a new chapter on SWATH-MS,
substantial updates to all chapters, including proteomic database
search and analytical quantification, expanded discussion of
post-hoc statistical tests, and additional content on validation in
proteomics.
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