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Books > Science & Mathematics > Chemistry > Analytical chemistry > Qualitative analytical chemistry > Chromatography
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Introduction to high performance liquid chromatography (Paperback, Softcover reprint of the original 1st ed. 1982)
Loot Price: R1,296
Discovery Miles 12 960
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Introduction to high performance liquid chromatography (Paperback, Softcover reprint of the original 1st ed. 1982)
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Since the first edition of this book the major advances have been
in column packings, where over ninety per cent of separations are
now performed using chemically bonded microparticulate packings,
and in instrumentation. The use of microprocessor control has
brought about a rationalization of mobile phase delivery systems
and in detectors, the introduction of electrochemical and
spectrophotometric detection other than in the ultra-violet region,
has widened the field of applications and the sensitivity of the
technique. The use of ion pair chromatography has increased at the
expense of ion-exchange and this together with the improvements in
detectors has greatly increased the appli cation of the technique
in the biomedical field. These advances are described together with
the established methods to enable the beginner to carry out a
satisfactory separation and to gain the experience necessary for
the full exploitation of the technique. R. J. Hamilton P. A. Sewell
Liverpool,1981 1 Introduction to high performance liquid
chromatography 1. 1 Introduction Chromatography in its many forms
is widely used as a separative and an analytical technique. Gas
chromatography since its introduction by James and Martin 1] has
been pre-eminent in the field. Uquid chromatography in the of
paper, thin-layer, ion-exchange, and exclusion (gel permeation and
gel form filtration) chromatography had not been able to achieve
the same success, mainly because of the poor efficiences and the
long analysis times arising from the low mobile phase flow rates."
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