PCR Sequencing Protocols (Spiral bound, 1996 ed.)

Advances in bioscience research usually arise as a result of the continu- ing refinement of existing technologies. However, there are a number of occa- sions v^rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu- tionary techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech- nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac- tion. This technique, first reported in 1985 by MuUis and his colleagues, pro- vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 65
Release date:	August 1996
First published:	1996
Editors:	Ralph Rapley
Dimensions:	229 x 152 x 18mm (L x W x T)
Format:	Spiral bound
Pages:	221
Edition:	1996 ed.
ISBN-13:	978-0-89603-344-3
Categories:	Books > Science & Mathematics > Biology, life sciences > Molecular biology Books > Science & Mathematics > Biology, life sciences > Cellular biology > General Books > Professional & Technical > Biochemical engineering > Biotechnology > Genetic engineering Promotions
LSN:	0-89603-344-9
Barcode:	9780896033443

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