Methods for Fragments Screening Using Surface Plasmon Resonance (Hardcover, 1st ed. 2021)

This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR.

General

Imprint:	Springer Verlag, Singapore
Country of origin:	Singapore
Release date:	September 2021
First published:	2021
Editors:	Sameer Mahmood Zaheer • Ramachandraiah Gosu
Dimensions:	235 x 155mm (L x W)
Format:	Hardcover
Pages:	113
Edition:	1st ed. 2021
ISBN-13:	978-981-16-1535-1
Categories:	Books > Science & Mathematics > Chemistry > General Books > Medicine > Other branches of medicine > Pharmacology > General Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
LSN:	981-16-1535-7
Barcode:	9789811615351

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