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Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Neurosciences
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Neurotrophic Factors - Methods and Protocols (Hardcover, 2nd ed. 2018)
Loot Price: R4,543
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Neurotrophic Factors - Methods and Protocols (Hardcover, 2nd ed. 2018)
Series: Methods in Molecular Biology, 1727
Expected to ship within 10 - 15 working days
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This volume collects protocols and procedures utilizing cellular,
tissue, and whole animal models that can be applied to the
investigation of neurotrophic factors and other agents impacting on
these systems. It begins with chapters on the culture of neurons
and glia from the central and peripheral nervous systems,
neuron-glia and glia-glia co-culture models, oligodendrocytes, and
cell-based assays for the evaluation of cell vitality. This revised
second edition expands to cover methodology encompassing
site-specific direct labeling of neurotrophins and their receptors,
angiogenesis assays, stem cells, pancreatic beta-cells, axonal
transport, synapse biology, dendritic spine analysis, and brain
endothelial cells. Subsequent chapters are dedicated to in vivo
lesion models of relevance to nervous system pathology, which can
be applied to the investigation of neurotrophic factors and
peptides, as well as protocols describing nanofiber- and
nanoparticle-based methods for brain delivery of neurotrophic
agents. Written for the highly successful Methods in Molecular
Biology series, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
practical, Neurotrophic Factors: Methods and Protocols, Second
Edition is a valuable resource for a wide audience of readers as
they explore nervous system function and pathology.
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