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Hydrocarbon and Lipid Microbiology Protocols - Petroleum, Hydrocarbon and Lipid Analysis (Hardcover, 1st ed. 2017)
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Hydrocarbon and Lipid Microbiology Protocols - Petroleum, Hydrocarbon and Lipid Analysis (Hardcover, 1st ed. 2017)
Series: Springer Protocols Handbooks
Expected to ship within 12 - 17 working days
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This Volume presents methods for analysing and quantifying
petroleum, hydrocarbons and lipids, based on their chemical and
physical properties as well as their biological effects. It
features protocols for extracting hydrocarbons from solid matrices,
water and air, and a dedicated chapter focusing on volatile organic
compounds. Several approaches for separating and detecting diverse
classes of hydrocarbons and lipids are described, including:
(tandem) gas chromatography (GC) coupled with mass spectrometry
(MS) or flame-ionisation detection, Fourier-transform
induction-coupled-resonance MS, and fluorescence-based techniques.
The book details high-performance liquid chromatography MS for
microbial lipids, as well as a combination of techniques for
naphthenic acids. Two chapters focus on quantifying bioavailable
hydrocarbon fractions by using cyclodextrin sorbents and bacterial
bioreporters, respectively, while a closing chapter explains how
compound-specific stable-isotope analysis can be used to measure
the fate of hydrocarbons in the environment. Hydrocarbon and Lipid
Microbiology Protocols There are tens of thousands of structurally
different hydrocarbons, hydrocarbon derivatives and lipids, and a
wide array of these molecules are required for cells to function.
The global hydrocarbon cycle, which is largely driven by
microorganisms, has a major impact on our environment and climate.
Microbes are responsible for cleaning up the environmental
pollution caused by the exploitation of hydrocarbon reservoirs and
will also be pivotal in reducing our reliance on fossil fuels by
providing biofuels, plastics and industrial chemicals. Gaining an
understanding of the relevant functions of the wide range of
microbes that produce, consume and modify hydrocarbons and related
compounds will be key to responding to these challenges. This
comprehensive collection of current and emerging protocols will
facilitate acquisition of this understanding and exploitation of
useful activities of such microbes.
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