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Hydrocarbon and Lipid Microbiology Protocols - Meso- and Microcosms (Hardcover, 1st ed. 2017)
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Hydrocarbon and Lipid Microbiology Protocols - Meso- and Microcosms (Hardcover, 1st ed. 2017)
Series: Springer Protocols Handbooks
Expected to ship within 12 - 17 working days
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This Volume describes methods for simulating natural environments
by using reproducible and controllable meso- and microcosm
experiments to analyse hydrocarbon-degrading microorganisms and to
test hypotheses. It presents important aspects of the preparation
of experimental set-ups, hydrocarbon application and sampling, and
features protocols for experiments with different types of samples,
such as biofilms, aquatic systems (e.g. groundwater, streams), and
sediments, including oscillating oxic-anoxic mesocosms, as well as
for in-situ experimentation in subtidal and deep sediments. Two
chapters are dedicated to cultivation under high-pressure
conditions, and several chapters include protocols for processing
samples for downstream chemical, microbial or activity analyses.
Several of the approaches presented are generic and will benefit
anyone embarking on designing meso- and microcosm experiments. <
Hydrocarbon and Lipid Microbiology Protocols There are tens of
thousands of structurally different hydrocarbons, hydrocarbon
derivatives and lipids, and a wide array of these molecules are
required for cells to function. The global hydrocarbon cycle, which
is largely driven by microorganisms, has a major impact on our
environment and climate. Microbes are responsible for cleaning up
the environmental pollution caused by the exploitation of
hydrocarbon reservoirs and will also be pivotal in reducing our
reliance on fossil fuels by providing biofuels, plastics and
industrial chemicals. Gaining an understanding of the relevant
functions of the wide range of microbes that produce, consume and
modify hydrocarbons and related compounds will be key to responding
to these challenges. This comprehensive collection of current and
emerging protocols will facilitate acquisition of this
understanding and exploitation of useful activities of such
microbes.
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