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Books > Science & Mathematics > Biology, life sciences > Microbiology (non-medical)
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Hydrocarbon and Lipid Microbiology Protocols - Field Studies (Hardcover, 1st ed. 2017)
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Hydrocarbon and Lipid Microbiology Protocols - Field Studies (Hardcover, 1st ed. 2017)
Series: Springer Protocols Handbooks
Expected to ship within 12 - 17 working days
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This Volume describes methods for investigating microbes in their
natural environment and how to obtain representative samples and
preserve them for subsequent analyses. Chapters are arranged
according to the environments under investigation, which include:
oil reservoirs, fracking fluids, aquifers, coal beds, oil sands and
their tailing ponds, lakes, rivers, leaves, polar seas and ice, the
sea-surface microlayer, mud flats, microbialites, and deep-sea
fauna. A variety of downstream analytical procedures are described,
including: nucleic-acid extraction and preparation for
high-throughput sequencing, fluorescence in-situ hybridisation, and
cultivation of aerobic and anaerobic hydrocarbon-degrading
microbes. Though most chapters focus on hydrocarbon-rich
environments, many of the approaches used are generic, and as such
will be of value to researchers embarking on studies of microbes
and their processes in the field. Hydrocarbon and Lipid
Microbiology Protocols There are tens of thousands of structurally
different hydrocarbons, hydrocarbon derivatives and lipids, and a
wide array of these molecules are required for cells to function.
The global hydrocarbon cycle, which is largely driven by
microorganisms, has a major impact on our environment and climate.
Microbes are responsible for cleaning up the environmental
pollution caused by the exploitation of hydrocarbon reservoirs and
will also be pivotal in reducing our reliance on fossil fuels by
providing biofuels, plastics and industrial chemicals. Gaining an
understanding of the relevant functions of the wide range of
microbes that produce, consume and modify hydrocarbons and related
compounds will be key to responding to these challenges. This
comprehensive collection of current and emerging protocols will
facilitate acquisition of this understanding and exploitation of
useful activities of such microbes.
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