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Hydrocarbon and Lipid Microbiology Protocols - Synthetic and Systems Biology - Applications (Hardcover, 1st ed. 2016)
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Hydrocarbon and Lipid Microbiology Protocols - Synthetic and Systems Biology - Applications (Hardcover, 1st ed. 2016)
Series: Springer Protocols Handbooks
Expected to ship within 12 - 17 working days
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This Volume presents protocols for systems and synthetic biology
applications in the field of hydrocarbon and lipid microbiology. It
complements another Volume that describes generic protocols for wet
experimental and computer-based systems and synthetic biology
studies. The protocols in this Volume demonstrate how to employ
systems and synthetic biology approaches in the design of microbes
for the production of esters, isoprenoids, hydrophobic polymers,
rhamnolipid biosurfactant, and peptide antimicrobial and
thioether-stabilised molecules. Also presented is a protocol for
the engineering of transcription factor-based biosensors for
intracellular products, and another for the creation of a synthetic
hydroxylase with novel activity for the selective
oxyfunctionalisation of linear alkanes. Hydrocarbon and Lipid
Microbiology ProtocolsThere are tens of thousands of structurally
different hydrocarbons, hydrocarbon derivatives and lipids, and a
wide array of these molecules are required for cells to function.
The global hydrocarbon cycle, which is largely driven by
microorganisms, has a major impact on our environment and climate.
Microbes are responsible for cleaning up the environmental
pollution caused by the exploitation of hydrocarbon reservoirs and
will also be pivotal in reducing our reliance on fossil fuels by
providing biofuels, plastics and industrial chemicals. Gaining an
understanding of the relevant functions of the wide range of
microbes that produce, consume and modify hydrocarbons and related
compounds will be key to responding to these challenges. This
comprehensive collection of current and emerging protocols will
facilitate acquisition of this understanding and exploitation of
useful activities of such microbes.
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