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Books > Science & Mathematics > Biology, life sciences > Microbiology (non-medical)
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Hydrocarbon and Lipid Microbiology Protocols - Synthetic and Systems Biology - Tools (Hardcover, 1st ed. 2016)
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Hydrocarbon and Lipid Microbiology Protocols - Synthetic and Systems Biology - Tools (Hardcover, 1st ed. 2016)
Series: Springer Protocols Handbooks
Expected to ship within 12 - 19 working days
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This Volume presents generic protocols for wet experimental and
computer-based systems and synthetic biology approaches relevant to
the field of hydrocarbon and lipid microbiology. It complements a
second Volume that describes protocols for systems and synthetic
biology applications. The wet experimental tools presented in this
Volume include protocols for the standardisation of transcriptional
measurements, application of uracil excision-based DNA editing for,
inter alia, multi-gene assembly, the use of fluxomics to optimise
"reducing power availability", and the incorporation of
non-canonical amino acids into proteins for optimisation of
activities. Phenome-ing microbes, using a combination of RNA-seq
and bioinformatic algorithms, is presented, as is an illustration,
using methylotrophs as an example, of how the different key omics
approaches constitute a pipeline for functional analysis,
acquisition of a systems overview, and metabolic optimisation.
Complementary computational tools that are presented include
protocols for probing the genome architecture of regulatory
networks, genome-scale metabolic reconstruction, and bioinformatic
approaches to guide metabolic engineering. The Volume also includes
an overview of how synthetic biology approaches can be used to
improve biocontainment. Hydrocarbon and Lipid Microbiology
ProtocolsThere are tens of thousands of structurally different
hydrocarbons, hydrocarbon derivatives and lipids, and a wide array
of these molecules are required for cells to function. The global
hydrocarbon cycle, which is largely driven by microorganisms, has a
major impact on our environment and climate. Microbes are
responsible for cleaning up the environmental pollution caused by
the exploitation of hydrocarbon reservoirs and will also be pivotal
in reducing our reliance on fossil fuels by providing biofuels,
plastics and industrial chemicals. Gaining an understanding of the
relevant functions of the wide range of microbes that produce,
consume and modify hydrocarbons and related compounds will be key
to responding to these challenges. This comprehensive collection of
current and emerging protocols will facilitate acquisition of this
understanding and exploitation of useful activities of such
microbes.
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