This Volume presents methods for quantifying microbial populations
and characterising microbial communities by extracting and
analysing biomarkers such as RNA, DNA and lipids. The chapters
cover a wide range of topics, including: cell separation from
oil-rich environments, enumeration of hydrocarbon degraders and
sulphate reducers using most-probable-number techniques, and
quantification by means of real-time PCR. A variety of molecular
methods are described for microbial community profiling, such as
phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One
chapter examines high-throughput sequencing, and provides important
information on the associated procedures required for thorough data
analysis. A further chapter is devoted to the characterisation of
protistan communities, while the closing chapter describes
multiplex fluorescent antibody microarrays for detecting microbial
biomarkers. Hydrocarbon and Lipid Microbiology Protocols There are
tens of thousands of structurally different hydrocarbons,
hydrocarbon derivatives and lipids, and a wide array of these
molecules are required for cells to function. The global
hydrocarbon cycle, which is largely driven by microorganisms, has a
major impact on our environment and climate. Microbes are
responsible for cleaning up the environmental pollution caused by
the exploitation of hydrocarbon reservoirs and will also be pivotal
in reducing our reliance on fossil fuels by providing biofuels,
plastics and industrial chemicals. Gaining an understanding of the
relevant functions of the wide range of microbes that produce,
consume and modify hydrocarbons and related compounds will be key
to responding to these challenges. This comprehensive collection of
current and emerging protocols will facilitate acquisition of this
understanding and exploitation of useful activities of such
microbes.
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