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Argonaute Proteins - Methods and Protocols (Hardcover, 2011)
Loot Price: R3,045
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Argonaute Proteins - Methods and Protocols (Hardcover, 2011)
Series: Methods in Molecular Biology, 725
Expected to ship within 10 - 15 working days
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With the rapid proliferation of RNAi applications in basic and
clinical sciences, the challenge has now become understanding how
components of RNAi machinery function together in a regulated
manner. Argonaute proteins are the central effectors of RNAi and
are highly conserved among eukaryotes and some archaebacteria.
These RNA-binding proteins use small guide RNAs to silence
expression of genes at the mRNA and DNA levels. In Argonaute
Proteins: Methods and Protocols, expert researchers in this
burgeoning field provide detailed, up-to-date methods to study
Argonaute protein functions and interactions in a wide variety of
cell types ranging from yeast to mammalian systems, as well as in
vitro. Written in the highly successful Methods in Molecular
Biology (TM) series format, chapters include brief introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and key tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Argonaute Proteins: Methods and
Protocols serves as a vital reference for both experienced and
novice scientists approaching the vast complexities of RNAi
research.
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