The series of books on the biotechnology of Medicinal and Aromatic Plants provides a survey of the literature, focusing on recent information and the state of the art in tissue culture and the in vitro production of secondary metabolites. This book, Medicinal and Aromatic Plants VII, like the previous six volumes published in 1988, 1989, 1991, 1993 and 1994, is unique in its approach. It comprises 28 chapters dealing with the distribu- tion, importance, conventional propagation, micro propagation, tissue culture studies, and the in vitro production of important medicinal and pharmaceutical compounds in various species of Aesculus, Althaea, Baptisia, Berberis, Beta, Bowiea, Camp to theca, Chrysanthellum, Citrus, Claviceps, Coleonema, Dianthus, Dunaliella, Epimedium, Euphorbia, Forsythia, Gomphrena, Larix, Lobelia, Medicago, Papaver, Phytolacca, Pueraria, Santalum, Santolina, Sapium, Tabebuia, and Tripterygium. This book is tailored to the needs of advanced students, teachers, and research scientists in the field of pharmacy, plant tissue culture, phytochemistry, biochemical engineering, and plant biotechnology in general. New Delhi, July 1994 Professor Y. P. S. BAJAJ Series Editor Contents I Aesculus hippocastanum L. (Horse Chestnut): In Vitro Culture and Production of Aescin P. GASTALDO, A. M. CAVIGLIA, and P. PROFUMO (With 7 Figures) 1 General Account ...1 ...2 In Vitro Culture Studies ...4 3 Summary and Conclusions ...10 4 Protocol...11 References ...11 II Althaea officinalis L. (Marshmallow): In Vitro Culture and the Production of Biologically Active Compounds I. IONKovA and A. W. ALFERMANN (With 10 Figures) 1 General Account...13 ...2 Biotechnological Approaches...21 .

Rice is the most important cereal crop which feeds more than half the population of the world. It is being grown in more than 144. 641 million ha with a production of over 468. 275 million tons (in 1988). Rice is attacked by a large number of pests and diseases which cause an enormous loss in its yield. Therefore, the major objectives in rice breeding are the development of disease resistance, tolerance to insects, adverse soil water, and drought; and improvement of quality including increased protein content. Tremendous efforts being made at the International Rice Research Institute have resulted in the release of improved varieties. It is estimated that the world's annual rice production must increase from 460 million tons (in 1987) to 560 million tons by the year 2000, and to 760 million tons by 2020 (a 65% increase) in order to keep up with the population growth (IRRI Rice Facts 1988). To achieve this gigantic goal, new strategies have to be evolved. Since the success of any crop improvement program de pends on the extent of genetic variability in the base population, new techniques need to be developed not only to generate the much needed variability but also for its conservation. In this regard the progress made in the biotechnology of rice during the last 5 years has amply demonstrated the immense value of innovative approaches for further improvement of this crop.

27 chapter cover the distribution, economic importance, conventional propagation, micropropagation, tissue culture, and in vitro production of important medicinal and pharmaceutical compounds in various species of Ajuga, Allium, Ambrosia, Artemisia, Aspilia, Atractylodes, Callitris, Choisya, Cinnamomum, Coluria, Cucumis, Drosera, Daucus, Eustoma, Fagopyrum, Hibiscus, Levisticum, Onobrychis, Orthosiphon, Quercus, Sanguinaria, Solanum, Sophora, Stauntonia, Tanecetum, Vetiveria, and Vitis. Like the previous volumes 4, 7, 15, and 21 in the Medicinal and Aromatic Plants series, the volume is tailored to the need of advanced students, teachers, and research scientists in the area of plant biotechnology andbioengineering, pharmacy, botany and biochemistry.

Somatic hybrids through the fusion of plant protoplasts have widened the genetic variability of cultivated plants. As "Somatic Hybridization in Crop Improvement I," published in 1994, this volume describes how this discipline can contribute to the improvement of crops. It comprises 24 chapters dealing with interspecific and intergeneric somatic hybridization and cybridization. It is divided into four sections:
I. Cereals: Barley, rice, and wheat.
II. Vegetables and Fruits: "Arabidopsis," "Asparagus," "Brassica," chicory, "Citrus," "Cucumis," "Diospyros," "Ipomoea," and various Solanaceous species, e.g., tomato, potato, and eggplant.
III. Medicinal and Aromatic Plants: "Atropa," "Dianthus," "Nicotiana," and "Senecio."
IV. Legumes/Pasture Crops: Alfalfa.
This book is tailored to the needs of advanced students, teachers and researchers in the fields of plant breeding, genetic engineering, and plant tissue culture.

There has been tremendous progress in the genetic transformation of agricultural crops, and plants resistant to insects, herbicides, and diseases have been produced, field tested, and patented. This book compiles this information on various fruits and vegetables.

Comprising 26 chapters, this volume deals with the genetic transformation of medicinal plants. It describes methods to obtain plants resistant to insects, diseases, herbicides, and plants with an increased production of compounds of medicinal and pharmaceutical importance. The plant species included are "Ajuga reptans," "Anthemis nobilis," "Astragalus" spp., "Atropa" "belladonna," "Catharanthus roseus," "Datura" spp., "Duboisia" species, "Fagopyrum" spp., "Glycyrrhiza" "uralensis," "Lobelia" spp., "Papaver" "somniferum," "Panax" "ginseng," "Peganum" "harmala," "Perezia" spp., "Pimpinella" "anisum," "Phyllanthus" "niruri," "Salvia" "miltiorrhiza," "Scoparia dulcis," "Scutellaria" "baicalesis," "Serratula" "tinctoria," "Solanum aculeatissimum," "Solanum " "commersonii," "Swainsona galegifolia," tobacco, and "Vinca minor."
This book is of special interest to advanced students, teachers, and researchers in the field of pharmacy, plant tissue culture, phytochemistry, molecular biology, biomedical engineering, and plant biotechnology in general.

Like the previous nine volumes published between 1988 and 1996, "Medicinal" "and" "Aromatic" "Plants X" is unique in its approach. It comprises 22 chapters dealing with the distribution, importance, conventional propagation, micropropagation, tissue culture studies, and the in vitro production of important medicinal and pharmaceutical compounds in various species of "Actinidia," "Alkanna," "Arnebia," "Campanula," "Catharanthus," "Centella," "Chenopodium," "Cornus," "Cynara," "Ephedra," "Euglena," "Haplophyllum," "Morus," "Oenothera," "Otacanthus," "Oxalis," "Polypodium," "Rosmarinus," "Sesamum," "Solanum," "Taxus," and "Tephrosia." This book is tailored to the needs of advanced students, teachers, and research scientists in the field of pharmacy, plant tissue culture, phytochemistry, biochemical engineering and plant biotechnology.

Forty chapters deal with various aspects of tissue culture, in vitro manipulation, and other biotechnological approaches to the improvement of maize.
They are arranged in eight sections: - In Vitro Technology, Callus Cultures and Regeneration of Plants, Somatic Embryogenesis. - Wide Hybridization, Embryo, Ovule, and Inflorescence Culture, in Vitro Fertilization. - Production of Haploids and Double Haploids, Anther and Pollen Culture. - Protoplast Culture, Genetic Transformation. - Somaclonal Variation and Mutations. - Molecular Biology and Physiological Studies. - Proteins and Nutritional Improvement. Pollen Storage, Cryopreservation of Germplasm.

Fantasies and dreams have their rightful place in science, and sometimes they turn into reality. Regeneration of hybrid plants through protoplast fusion is one such dream come true. In the early 1970s I shared the pioneering excitement in the field of protoplast technology at the Second International Congress of Plant Tissue Culture held in Strasbourg, France. Subsequently, I participated in three international conferences devoted to plant protoplasts, in Salamanca, Spain (1972), Versailles, France (1972), and Nottingham, England (1975). At Versailles Dr. P.S. Carlson presented his work on the successful regeneration of somatic hybrids between Nicotiana glauca and Nicotiana langsdorfii. The enthusi- asm shown by the participants was sufficient indication of the bright future of somatic hybridization. On my return from Versailles, I gathered my thoughts and prepared a concept paper on Potentials of Protoplast Culture Work in Agriculture which was published in Euphytica (Bajaj 1974). The studies on protoplast fusion and somatic hybridization then gained momentum and active work started in many laboratories. Very significant work was done by Melchers et al. (1978) who obtained a somatic hybrid between potato and tomato, calling it "Pomato".

Plant protoplasts have proved to be an excellent tool for in vitro manipu- lations, somatic hybridization, DNA uptake and genetic transformation, and for the induction of somaclonal variation. These studies reflect the far- reaching impact of protoplast alterations for agriculture and forest bio- technology. Taking these aspects into consideration, the series of books on Plant Protoplasts and Genetic Engineering provides a survey of the litera- ture, focusing on recent information and the state of the art in protoplast Plant Protoplasts manipulation and genetic transformation. This book, and Genetic Engineering VI, like the previous five volumes published in 1989,1993, and 1994, is unique in its approach. It comprises 27 chapters dealing with the regeneration of plants from protoplasts, and genetic transformation in various species of Arachis, Bupleurum, Capsella, Dendrobium, Dianthus, Diospyros, Fagopyrum, Festuca, Gentiana, Glycyrrhiza, Gossypium, Hemerocallis, Levisticum, Lonicera, Musa, Physallis, Platanus, Prunus, Saposhnikovia, Solanum, Spinacia, Trititrigia, Tulipa, and Vaccinium; including fruits such as apricot, banana, cranberry, pepino, peach, and plum. This book may be of special interest to advanced students, teachers, and research scientists in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general bio- technology. New Delhi, August 1995 Professor Y. P. S. BAJA] Series Editor Contents Section I Regeneration of Plants from Protoplasts 1. 1 Regeneration of Plants from Protop1asts of Arachis Species (Peanut) Z. LI, R. L. JARRET, and J. W. DEMSKI (With 2 Figures) 1 Introduction ...3 2 Isolation of Pro top lasts ...4 3 Culture of Protoplasts ...

27 chapters cover the distribution, economic importance, conventional propagation, micropropagation, tissue culture studies, and in vitro production of important medicinal and other pharmaceutical compounds in various species of Anchusa, Brucea, Catharanthus, Chrysanthemum, Coleus, Corydalis, Coreopsis, Emilia, Ginkgo, Gloriosa, Hypericum, Inonotus, Leucosceptrum, Lilium, Linum, Mosses, Nandina, Penstemon, Prunus, Pteridium, Quassia, Ribes, Senecio, Taraxacum, Thermopsis, Vanilla, and Vitiveria. Like the previous five volumes on medicinal and aromatic plants (Volumes 4, 7, 15, 21, and 24), this book contains a wealth of useful information for advanced students and researchers in the field of plant biotechnology and chemical engineering, pharmacy, botany and tissue culture.

Haploid plants have the gametophytic number of chromosomes. They are of great importance, especially in studies on the induction of muta tions and also for the production of homozygous plants, they are needed in large numbers. The conventional methods employed by plant breeders for their production are cumbersome, time-consuming, laborious and rather inefficient. Sometimes it may take years to produce a pure line. However, with the introduction of in vitro techniques, especially anther culture for the induction of androgenesis, it has become increasingly evi dent that these methods considerably accelerate the production of haploids for plant breeding programs. During the last decade, in vitro-produced haploids have been incor porated into breeding programs of many agricultural crops, and positive results have been obtained especially with rice, wheat, potato, barley, maize, asparagus, sunflower, brassica, tobacco, etc. Among these, rice and wheat are the best examples in which a number of improved varieties have been released. In wheat, the breeding cycle can be shortened by three or four generations when the pollen haploid breeding method is used instead of conventional cross-breeding. The release of the wheat varieties Jinghua 1 and Florin is a typical example of what can be achieved with other crops. Taking these developments into considera tion, the present volume, Haploids in Crop Improvement I, was compil ed.

After the 1988 and 1989 volumes, this is the third volume on Medicinal and Aromatic Plants. Each of the 29 chapters contributed by international scientists deals with one individual plant genus, namely "Atropa," "Ageratina," "Ailanthus," "Aconitum," "Apium," "Aloe," "Akebia," "Bidens," "Carthamus," "Chamomilla," "Carum," "Citrus," "Cymbopogon," "Dysosma," "Euphorbia," "Fritillaria," "Glycyrrhiza," "Lavandula," "Nigella," "Pelargonium," "Perilla," "Podophyllum," "Rosa," "Scutellaria," "Securinega," "Solanum," "Swertia," "Symphytum," "Syringa." Their distribution, economic importance, conventional propagation, in-vitro propagation and production of metabolites through tissue culture are treated in detail. Special emphasis is laid on the potential of industrial in-vitro production of plant compounds of medical and pharmaceutical relevance using tissue culture.

Cotton is a multipurpose crop and produces lint, the most important source of fiber used in the textile industry, oil, seed meal, and hulls.
Twenty-three chapters on various aspects of in vitro manipulation and other biotechnological approaches to the improvement of cotton are arranged in six sections. Special emphasis is placed on interspecific hybridization, somaclonal variation, transgenic cotton resistant to insects and herbicides, and re-engineering of fiber.
This book is of special interest to advanced students, teachers, and research workers in the field of cotton breeding, genetics, tissue culture, molecular biology, and plant biotechnology in general.

Genetic engineering through DNA recombinants and the in vitro manipulation of isolated protoplasts has recently attracted much atten- tion in agricultural biotechnology, and has greatly advanced during the last 5 years. In an earlier book, Plant Protoplasts and Genetic Engineer- ing I, methods for the isolation, fusion and culture of protoplasts were reviewed and the regeneration of complete plants from isolated pro- toplasts of rice, potato, soybean, linseed, cabbage, chicory, lettuce, but- terbur, orchids, citrus and some other tree species, and interspecific and intergeneric somatic hybrids in Lycopersicon, Petunia, Nicotiana, Solanum, Glycine, Citrus, Brassica, Medicago and Trifolium spp. were discussed. The present volume, Plant Protoplasts and Genetic Engineering II, deals with some of the newer techniques such as microinjections, elec- trofusion, flow cytometry, uptake and integration of DNA, nuclei, iso- lated chromosomes by plant protoplasts and the subsequent regeneration of transgenic plants. The literature on the DNA recombinants and genetic transformation, both Agrobacterium-mediated and direct gene transfer in agricultural crops and trees, such as poplars, is reviewed, and the uses of cytoplasts and miniprotoplasts in genetic manipulation are highlighted.

In continuation of Volumes 8, 9, and 22 on in vitro manipulation of plant protplasts, this new volume deals with the regeneration of plants from protoplasts and genetic transformation in various species of Actinidia, Amoracia, Beta, Brassica, Cicer, Citrus, Cucumis, Duboisia, Fragaria, Glycine, Ipomoea, Lactuca, Lotus, Lycopersicon, Manihot, Medicago, Nicotiana, Petunia, Phaseolus, Pisum, Prunus, Psophocarpus, Saccharum, Solanum, Sorghum, Stylosanthes, and Vitis. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. They are of special interest to researchers in the field of plant tissue culture, molecular biology, genetic engineering, and plant breeding.

Plants are a major source of medicines, flavors, fragrances, and various pharmaceutical and industrial products. Biotechnology is being put to the service for mass clonal propagation of plants, and to produce impor- tant secondary products in cell cultures. In some cases cell cultures ac- cumulate higher amounts of products than the intact plant cells in situ, and such cultures can be stored through immobilization and cryopreser- vation. An in vitro-produced anti-inflammatory drug, shikonin, has been commercialized, and the recent observations on the increased pro- duction of atropine and hyoscyamine by Agrobacterium rhizogenes- mediated transformed "hairy roots" have encouraged the acceptance of such biotechnologies by the pharmaceutical industry. In an earlier volume, Medicinal and Aromatic Plants I, various aspects of in-vitro culture of cells, bioreactors, micropropagation, im- mobilization, and cryopreservation were discussed. The present volume concerns the application of these biotechnologies to 29 genera of medicinal and aromatic plants. It deals with the distribution, economic importance, conventional propagation, micro propagation, review of tissue culture studies, and the in-vitro production of important medicinal and pharmaceutical compounds in various species of Angelica, Anisodus, Basel/a, Bupleurum, Camellia, Co ix, Coptis, Cryp- tomeria, Datura, Dioscorea, Foeniculum, Gardenia, Geigeria, Heimia, Humulus, Hyoscyamus, Jasminum, Macleaya, Mucuna, Nicotiana, Pimpinel/a, Rauwolfia, Ruta, Salvia, So/anum, Saponaria, Stevia, Tabernaemontana, and Zingiber. The potential role of biotechnology for industrial production is discussed. Biotechnology enables the production and isolation of products of higher purity and also opens the possibility of making desired molecular alterations in products.

Second in the series, High-Tech and Micropropagation, this work covers the micropropagation of trees and fruit-bearing plants, such as poplar, birches, larch, American sweetgum, black locust, "Sorbus," sandalwood, "Quercus," cedar, Persian walnut, date palm, cocoa, "Citrus," olive, apple, pear, peach, plum, cherry, papaya, pineapple, kiwi, Japanese persimmon, grapevine, strawberry, and raspberry. The importance and distribution of conventional propagation and in vitro studies on individual species are discussed. In particular detail, the transfer of in vitro plants to the greenhouse or the field, and the prospects of commercial exploitation are examined. The book will be of use to advanced students, research workers and teachers in horticulture, forestry and plant biotechnology in general, and also to individuals interested in industrial micropropagation.

While working in the laboratory of Professor Dr. Jacob Reinert at the Freie Universitat Berlin (1974-1976), I had the opportunity to become deeply involved in studying the intricacies of the fascinating phenomenon of somatic embryogenesis in plant cells and protoplasts. In numerous stimu lating discussions with Professor Reinert on this subject, I was fully convinced that somatic embryogenesis would become one of the most important areas of study, not only regarding basic and fundamental aspects, but also for its application in crop improvement. During the last decade, we have witnessed tremendous interest and achievements in the use of somatic embryos for the production of synthetic seeds, for micro prop a gation, genetic transformation, cryopreservation, and conservation of germplasm. The en masse production of somatic embryos in the bioreactors has facilitated some of these studies. Somatic embryos have now been induced in more than 300 plant species belonging to a wide range offamilies. It was therefore felt that a compilation ofliterature/state of the art on this subject was necessary. Thus, two volumes on Somatic Embryo genesis and Synthetic Seed have been compiled, which contain 65 chapters contributed by International experts. Somatic Embryogenesis and Synthetic Seed I comprises 31 chapters, arranged in 3 sections: Section I Commitment of the cell to somatic embryogenesis; early events; anatomy; molecular basis; gene expression; role of polyamines; machine vision analysis of somatic embryos. Section II Applications of somatic embryos; technology of synthetic seed; fluid drilling; micropropagation; genetic transfor mation through somatic embryos; cryopreservation."

Deals with biotechnological approaches incorporated into po- tato improvement progammes. These methods have far-reaching implications for the synthesis of improved, disease- resist- ant and nutritious cultivars of potato.

The increasing use of biotechnology for the improvement of agricultural crop species has gained momentum, and recent developments (see Crops J) have shown beyond doubt the far-reaching implications of biotechnological approaches for future agricultural research and plant- breeding programs. The production of novel plants and somaclones showing resistance to pests, diseases, herbicides, and salt and the early release of disease-free as well as improved cultivars have become reality. The present volume comprises 31 chapters and deals with the impor- tance, distribution, conventional propagation, micropropagation, and methods for the in-vitro induction of genetic variability in various fruits, vegetables, grasses, and pasture crops such as grapes, strawberry, brambles, red raspberry, currants, gooseberry, kiwifruit, blueberry, cran- berry, cauliflower, cabbage, brussels sprouts, broccoli, cucumber, chico- ry, taro, rhubarb, lettuce, spinach, quinoa, kale, fescue, bromegrass, Ber- mudagrass, napier grass, foxtail millet, turtle grass and others. (The cere- als and other vegetable crops are discussed in Crops J, Vol. 2 of the series). Micropropagation of some fruit crops such as strawberry, grape, and raspberry is already being practiced on a large scale in various countries. Likewise, test-tube-derived plants of certain crops such as brassicas, let- tuce, and taro and improved pastures are being utilized, while the technology for mass propagation of certain other crops is being worked out. These recent developments emphasize the urgent need to arouse awareness among horticultural scientists and plant breeders to enable them to incorporate these modern innovative approaches into routine crop improvement programs.

Legumes and oilseed crops are a rich source of protein and energy. The major objectives in grain-legume breeding are to increase grain yield, protein quantity and quality and digestibility, and to decrease toxic fac- tors, seed hardness and cooking time. Similarly, oilseed crops such as brassicas and peanut share somewhat similar genetic improvement goals, but suffer from susceptibility to a number of pathogens and diseases. In spite of the best efforts through conventional breeding, their yield has been virtually static, with no significant breakthrough. The lack of genetic variability in the base population has been considered to be a major limiting factor for the slow progress made in the improvement of these crops. The desired goals can be achieved by incorporating addi- tional genetic variability in the existing germplasm. The protein and oil contents which are genotypically oriented can be enhanced further by suitable crosses. In this regard, progress made during the last decade, in the area of in vitro manipulation and recombinant DNA technology, holds promise for the improvement of these crops. Among the oilseed crops, Brassica spp. , soybean and sunflower have been well studied, wherein haploids, somaclones, somatic hybrids, cybrids and transformed plants have been produced. Oilpalm is one of the best examples where micropropagation is being commercially prac- ticed. Regarding legumes, though there is extensive work on forages, such as Medicago and Trifolium, much needs to be done on food legumes.

Isolated protoplasts are a unique tool for genetic manipulation of plants. Since the discovery of a method for the enzymatic isolation of pro-. toplasts by Professor E. C. Cocking in 1960, tremendous progress has been made in this very fascinating area of research. I have witnessed the struggle in the 1960's and early 1970's, when obtaining a clean prepara tion of protoplasts was considered an achievement. I also shared the pioneering excitement and enthusiasm in this field during the 2nd Inter national Congress of Plant Tissue Culture held at Strasbourg in 1970, where Dr. I. Thkebe of Japan presented his work on the induction of division in tobacco protoplasts. This was followed by my participation in three international conferences devoted to plant protoplasts held in 1972 in Salamanca (Spain) and Versailles (France), and then in 1975 in Nottingham (England). The enthusiasm shown by plant scientists at these meetings was ample proof of the bright future of protoplast technology, and it became evident that protoplasts would playa major role in plant biotechnology, especially in genetic engineering. Since then we have never looked back, and now the methods for isolation, fusion, and culture, as well as regeneration of somatic hybrids, have become routine laboratory procedures for most plant species. Currently the focus is on cereal and tree protoplasts. In order to bring about any genetic manipulation through fusion, in corporation of DNA, and transformation, the regeneration of the entire plant through manipulation of protoplasts is a prerequisite."

Two aspects of the biotechnology of medicinal and aromatic plants are of immediate application. (1) Micropropagation under controlled germ- free conditions which enables their fast multiplication and availability throughout the year irrespective of external environment - this is specially useful for elite and rare plants. (2) A large-scale culture and low-temperature storage of cells enables retention of their biosynthetic potential for the production of important secondary metabolites, med- icines, flavours and other pharmaceutical products. This book has been compiled with a view to bringing together information and literature on the biotechnology and the present state of the art of plant cell cultures for their potential use in the pharmaceutical industry. This volume comprises 29 chapters on the biotechnology of med- icinal and aromatic plants grouped into three sections, (1) microprop- agation, immobilization, cryopreservation, bioreactors, production of secondary metabolites and their impact in pharmacy, (2) production through cell cultures of antitumour compounds, lrDopa, shikonin, an- thraquinone, morphinan alkaloids, caffeine, berberine, valeoptriates, rosmarinic acid, quinine, tropanes, hypoxoside, ellipticine, paeoniflorin, saponins, cardenolides etc, and (3) distribution, economic importance, conventional propagation, review of the tissue culture work on micropropagation and the in vitro production of compounds of medicinal and pharmaceutical interest in various species of Cannabis, Centaurium, Cinchona, Digitalis, Duboisia, Hypoxia, Lithospermum, Ochrosia, Paeonia, Panax, Papavar, Rehmannia, Rhamnus and Rhaza.

Recently, the pharmaceutical industry has shown considerable interest in the en masse micropropagation of elite plants, and the large-scale produc- tion of secondary metabolites in plant cell cultures through the use of bioreactors. This is now being realized by the selection of high-yielding somac1ones or through the production of hairy roots by transformation with Agrobacterium rhizogenes. These new developments need to be high-lighted and brought to the attention of workers dealing with medicinal, aromatic, and other plants of industrial importance. A series of books on the biotech- nology of medicinal and aromatic plants is therefore being compiled to provide a survey of the literature focusing on recent information and the state of the art. This book, Medicinal and Aromatic Plants IV, like the previous three volumes (Medicinal and Aromatic Plants I, II, and III, published in 1988, 1989, and 1991, respectively) is unique in its approach. It comprises 28 chapters dealing with the distribution, economic importance, conventional propagation, review of tissue culture studies, and the in vitro production of important medicinal and pharmaceutical compounds in various species of Ammi, Bergenia, Canavalia, Capsicum, Cassia, Cephaelis, Cornus, Cucurbita, Elettaria, Eupatorium, Genipa, Gentiana, Gypsophila, Hygrophila, Leon- topodium, Nerium, Picrasma, Polygonum, Ptelea, Rheum, Scopolia, Silene, Solanum, Strophanthus, Tagetes, Thymus, and Uncaria. This book is tailored to the needs of advanced students, teachers, and research workers in the fields of plant biotechnology and chemical engineer- ing, pharmacy, botany, and phytochemistry.