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Plant Protoplasts and Genetic Engineering II (Paperback, Softcover reprint of the original 1st ed. 1989)
Loot Price: R2,874
Discovery Miles 28 740
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Plant Protoplasts and Genetic Engineering II (Paperback, Softcover reprint of the original 1st ed. 1989)
Series: Biotechnology in Agriculture and Forestry, 9
Expected to ship within 10 - 15 working days
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Total price: R2,894
Discovery Miles: 28 940
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Genetic engineering through DNA recombinants and the in vitro
manipulation of isolated protoplasts has recently attracted much
atten- tion in agricultural biotechnology, and has greatly advanced
during the last 5 years. In an earlier book, Plant Protoplasts and
Genetic Engineer- ing I, methods for the isolation, fusion and
culture of protoplasts were reviewed and the regeneration of
complete plants from isolated pro- toplasts of rice, potato,
soybean, linseed, cabbage, chicory, lettuce, but- terbur, orchids,
citrus and some other tree species, and interspecific and
intergeneric somatic hybrids in Lycopersicon, Petunia, Nicotiana,
Solanum, Glycine, Citrus, Brassica, Medicago and Trifolium spp.
were discussed. The present volume, Plant Protoplasts and Genetic
Engineering II, deals with some of the newer techniques such as
microinjections, elec- trofusion, flow cytometry, uptake and
integration of DNA, nuclei, iso- lated chromosomes by plant
protoplasts and the subsequent regeneration of transgenic plants.
The literature on the DNA recombinants and genetic transformation,
both Agrobacterium-mediated and direct gene transfer in
agricultural crops and trees, such as poplars, is reviewed, and the
uses of cytoplasts and miniprotoplasts in genetic manipulation are
highlighted.
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