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Plant Protoplasts and Genetic Engineering I (Paperback, Softcover reprint of the original 1st ed. 1989)
Loot Price: R2,857
Discovery Miles 28 570
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Plant Protoplasts and Genetic Engineering I (Paperback, Softcover reprint of the original 1st ed. 1989)
Series: Biotechnology in Agriculture and Forestry, 8
Expected to ship within 10 - 15 working days
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Total price: R2,867
Discovery Miles: 28 670
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Isolated protoplasts are a unique tool for genetic manipulation of
plants. Since the discovery of a method for the enzymatic isolation
of pro-. toplasts by Professor E. C. Cocking in 1960, tremendous
progress has been made in this very fascinating area of research. I
have witnessed the struggle in the 1960's and early 1970's, when
obtaining a clean prepara tion of protoplasts was considered an
achievement. I also shared the pioneering excitement and enthusiasm
in this field during the 2nd Inter national Congress of Plant
Tissue Culture held at Strasbourg in 1970, where Dr. I. Thkebe of
Japan presented his work on the induction of division in tobacco
protoplasts. This was followed by my participation in three
international conferences devoted to plant protoplasts held in 1972
in Salamanca (Spain) and Versailles (France), and then in 1975 in
Nottingham (England). The enthusiasm shown by plant scientists at
these meetings was ample proof of the bright future of protoplast
technology, and it became evident that protoplasts would playa
major role in plant biotechnology, especially in genetic
engineering. Since then we have never looked back, and now the
methods for isolation, fusion, and culture, as well as regeneration
of somatic hybrids, have become routine laboratory procedures for
most plant species. Currently the focus is on cereal and tree
protoplasts. In order to bring about any genetic manipulation
through fusion, in corporation of DNA, and transformation, the
regeneration of the entire plant through manipulation of
protoplasts is a prerequisite."
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