This volume covers various aspects of affinity chromatography along with examples of its applications. Chapters guide readers through methodologies to purify a diverse array of molecular targets such as antibodies, extracellular vesicles, recombinant proteins, biomarkers, metabolites, plant organelles, nucleic acids, ligand identification and protocols on building affinity matrix. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Affinity Chromatography: Methods and Protocols aims to be an invaluable resource to anyone employing affinity chromatography-based methodologies.

At present where protein identification and characterisation using mass spectrometry is a method of choice, this book is presenting a review of basic proteomic techniques. The second part of the book is related to the novel high throughput protein identification technique called the 'molecular scanner'.

Several protein identification techniques are described, especially the peptide mass fingerprint with MALDI-MS based method. E.g. ionisation process, matrix available, signal reproducibility and suppression effect, as well as date treatment for protein identification using bioinformatics tools.

This classic textbook is an introduction to the systematics and the use of stable isotopes in geosciences. It is subdivided into three parts: i) theoretical and experimental principles, ii) fractionation processes of light and heavy elements, iii) the natural variations of geologically important reservoirs. Since the publication of the previous edition improvements in multi-collector ICP mass-spectrometry have increased the ability to measure isotope ratios with very high precision for many elements of the periodic table. The amount of published data has increased tremendously in the last years; thus, conclusions based on a limited database are now better constrained. In this new edition, therefore, 47 elements with resolvable natural variations in isotope composition are discussed. This increase of elements, together with advances in the calculation of equilibrium isotope fractionation using ab initio methods, has led to an unbelievable rise of publications, making substantial major revisions and extensions of the last edition necessary. Many new references have been added, which enable quick access to recent literature.

This volume is devoted to the physics, instrumentation and analytical methods of secondary ion mass spectroscopy (SIMS) in relation to solid surfaces. It describes modern models of secondary ion formation and the factors influencing sensitivity of measurements and the range of applications. All the main parts of SIMS instruments are discussed in detail. Emphasising practical applications the book also considers the methods and analytical procedures for constitutional analysis of solids --- including metals, semiconductors, organic and biological samples. Methods of depth profiling, spatially multidimensional analysis and study of processes at the surface, such as adsorption, catalysis and oxidation, are given along with the application of SIMS in combination with other methods of surface analysis.

The series Topics in Current Chemistry Collections presents critical reviews from the journal Topics in Current Chemistry organized in topical volumes. The scope of coverage is all areas of chemical science including the interfaces with related disciplines such as biology, medicine and materials science. The goal of each thematic volume is to give the non-specialist reader, whether in academia or industry, a comprehensive insight into an area where new research is emerging which is of interest to a larger scientific audience.Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field.Chapters "Metal Peptide Conjugates in Cell and Tissue Imaging and Biosensing", "Luminescent Metal Complexes as Emerging Tools for Lipid Imaging" and "Determination and Imaging of Small Biomolecules and Ions Using Ruthenium(II) Complex-Based Chemosensors" are available open access under a CC BY 4.0 License via link.springer.com.

The objective of the Springer Handbook of Enzymes is to provide, in concise form, data on enzymes that have been sufficiently well characterized. Data sheets are arranged in their EC-Number sequence. Each volume comprises one enzyme class, sometimes the enzyme classes have to be divided into several volumes. Considerable progress has been made in enzymology since the publication of the first edition (published as "Enzyme Handbook"): many enzymes are newly classified or reclassified. In the 2nd edition each entry is correlated with references and one or more source organisms. New datafields are created: "application" and "engineering" (for the properties of enzymes where the sequence has been changed). Altogether the amount of data has doubled so that the 2nd edition will consist of approx. 25 volumes. This collection is an indispensable source of information for researchers in biochemistry, biotechnology, organic and analytical chemistry, and food sciences.  

Chemical vapor sensing arrays have grown in popularity over the past two decades, finding applications for tasks such as process control, environmental monitoring, and medical diagnosis. This is the first in-depth analysis of the process of choosing materials and components for these "electronic noses", with special emphasis on computational methods. For a view of component selection with an experimental perspective, readers may refer to the complementary volume of Integrated Microanalytical Systems entitled "Combinatorial Methodologies for Sensor Materials."

This book provides a comprehensive overview on the recent significant advancements of conductive polymers and their composites in terms of conductive mechanism, fabrication strategies, important properties, and various promising applications. The corresponding knowledge was systematically compiled in the logical order and demonstrated as seven chapters. The special structure, influencing factors of the conductivity, the charge carrier transport model, the wettability and classical categories of the conductive polymers are narrated. Both conventional and novel strategies undertaken to fabricate the conductive polymers are introduced, as provided the overall master of the progress. In comparison with the bulk counterpart, nanostructured conductive polymers with different dimensions such as nanospheres, nano-networks, nanotubes and nanowire arrays are produced through distinct methods, thus presenting unique and distinct performance endowed by the nanometer scale. The combination of conductive polymers with other functional materials results in a number of the composites with improved properties by synergistic effect. The superior performance of conductive polymers and their composites greatly facilitates their development toward various important applications in the advanced and sophisticated fields such as biological utilization, energy storage and sensors. Due to their excellent biocompatibility, conductive polymers and their composites stand out to be useful in the biological field including tissue engineering, drug delivery and artificial muscle. To meet the urgent demand of the energy storage, conductive polymers and their composites play an important role in the devices including supercapacitors, solar cells and fuel cells. Finally, development of conductive polymers and their composites in the modern industry is greatly enhanced by their applications in smart sensors such as conductometric sensors, gravimetric sensors, optical sensors, chemical sensors and biosensors. This book has significant value for researchers, graduate students, and engineers carrying out the fundamental research or industrial production of conductive polymers and their composites.

Most of the recent texts on compact modeling are limited to a particular class of semiconductor devices and do not provide comprehensive coverage of the field. Having a single comprehensive reference for the compact models of most commonly used semiconductor devices (both active and passive) represents a significant advantage for the reader. Indeed, several kinds of semiconductor devices are routinely encountered in a single IC design or in a single modeling support group. Compact Modeling includes mostly the material that after several years of IC design applications has been found both theoretically sound and practically significant. Assigning the individual chapters to the groups responsible for the definitive work on the subject assures the highest possible degree of expertise on each of the covered models.

This first book on high-speed atomic force microscopy (HS-AFM) is intended for students and biologists who want to use HS-AFM in their research. It provides straightforward explanations of the principle and techniques of AFM and HS-AFM. Numerous examples of HS-AFM studies on proteins demonstrate how to apply this new form of microscopy to specific biological problems. Several precautions for successful imaging and the preparation of cantilever tips and substrate surfaces will greatly benefit first-time users of HS-AFM. In turn, the instrumentation techniques detailed in Chapter 4 can be skipped, but will be useful for engineers and scientists who want to develop the next generation of high-speed scanning probe microscopes for biology. The book is intended to facilitate the first-time use of this new technique, and to inspire students and researchers to tackle their own specific biological problems by directly observing dynamic events occurring in the nanoscopic world. Microscopy in biology has recently entered a new era with the advent of high-speed atomic force microscopy (HS-AFM). Unlike optical microscopy, electron microscopy, and conventional slow AFM, it allows us to directly observe biological molecules in physiological environments. Molecular "movies" created using HS-AFM can directly reveal how molecules behave and operate, without the need for subsequent complex analyses and roundabout interpretations. It also allows us to directly monitor morphological change in live cells, and dynamic molecular events occurring on the surfaces of living bacteria and intracellular organelles. As HS-AFM instruments were recently commercialized, in the near future HS-AFM is expected to become a common tool in biology, and will enhance and accelerate our understanding of biological phenomena.

This thesis provides unique information on the Kerr-lens mode-locking (KLM) technique applied to a thin-disk laser. It describes in detail cavity geometry, the qualitative approach to KLM, and self-starting behavior in the regime of both negative and positive dispersion. Comprehensive comparative analysis of KLM and semiconductor saturable absorber techniques is also carried out. Recent successful experiments on carrier-envelope phase stabilization, spectral broadening and compression of output of this oscillator underline the importance of this new, emerging technology.

The use of silicon-based microsystems for chemical analysis is one of the most promising concepts in the recent developments in micro system technology (MST). It is expected that chemical sensors will be increasingly integrated in so-called miniaturized total analysis systems (uTAS), a concept first presented by Ciba-Geigy. In such systems, all steps in a chemical determination, from sampling to detection and data treatment, are integrated in one miniature instrument. uTAS offer a variety of advantages over conventional analysis systems such as improved analytical performance, reduced reagent and power consumption, small size, possibility of new and more complicated functions, higher reliability and lower fabrication costs. Application of uTAS may be found in fields like process industry, environmental monitoring, medical diagnostics, aeronautics, automotive industry, and more.

Nanoanalytics is a novel branch of analytical chemistry which explores applications of nanotechnologies in chemical analysis. This comprehensive publication gives an overview of the analytical techniques used to study nanoobjects and nanoparticles as well as the application of nanomaterials themselves in the development of new methods of analysis. The authors also address important metrology aspects and give future prospects of the area.

This volume offers an overview of the occurrence and distribution of personal care products in continental and marine waters, presents analytical methods and degradation technologies and discusses their impact on human health. Experts from different disciplines highlight major issues for each family of compounds related to their occurrence in the water column as well as in solid and biota samples, methodological strategies for their analysis, non-conventional degradation technologies, (eco)toxicity data and their human and environmental risk assessment. The book also includes a general introduction to personal care products, covering their properties, use, behaviour and regulatory framework, and a final chapter identifying knowledge gaps and future research trends. It will appeal to experts from various fields of research, including analytical and environmental chemistry, toxicology and environmental engineering.

NMR is better suited than any other experimental technique for the characterization of supramolecular systems in solution. The presentations included here can be broadly divided into three classes. The first class illustrates the state of the art in the design of supramolecular systems and includes examples of different classes of supramolecular complexes: catenanes, rotaxanes, hydrogen-bonded rosettes, tubes, capsules, dendrimers, and metal-containing hosts. The second class comprises contributions to NMR methods that can be applied to address the main structural problems that arise in supramolecular chemistry. The third class includes biological supramolecular systems studied by state-of-the-art NMR techniques.

The properties and nature of water clusters studied with novel spectroscopic approaches are presented in this thesis. Following a general introduction on the chemistry of water and water clusters, detailed descriptions of the experiments and analyses are given. All the experimental results, including first size-selective spectra of large clusters consisting of 200 water molecules, are presented with corresponding analyses. Hitherto unidentified hydrogen bond network structures, dynamics, and reactivity of various water clusters have been characterized at the molecular level. The main targets of this book are physical chemists and chemical physicists who are interested in water chemistry or cluster chemistry.

This book presents the meaning of green infrastructure and its concerns to the contribution of materials and applications. It explores the evolving contested material under "green infrastructure" covering timber, concrete, soil, and pavement. It discusses the resistance to the ambiguity of managing the construction of green infrastructure and drawing on wider debates around applications and processes on construction. These contributions are by no means definitive, but rather an attempt to provide a detached and holistic perspective on the engineering "green infrastructure" concept.

When developing new food products, it is important to understand the major mechanisms of food macromolecule interactions. The first edition of this book provided a useful scientific and theoretical base describing the reasons for polymer action. The second edition updates the substantial progress that has occurred during the last ten years in many aspects of understanding, measuring, and utilizing functional macromolecules. The shift to analyzing mixtures rather than single polymers is assessed and the relevant interactions that are known to take place between the large molecules are examined. A new chapter on high pressure processing is included to show the importance of new methodology to texturize proteins. The book is an essential reference for food scientists who need to understand the fundamental principles that underlie functional behavior of interacting food macromolecules.

New developments in mass spectrometry have allowed routine identification and lowered limits of detection at levels only imagined a decade ago. Thousands of contaminants and residues in the food supply and the environment are now being reported. Between 2005 and 2010, more than 5,000 publications covering TOF-MS and environmental and food analysis were published, showing the importance of the technique in these applications.

This book covers the basic principles of method development in GC- and LC-TOF-MS as well as the main operational parameters related to TOF-MS. The second part focuses on the relevant environmental applications, including quality control aspects as well as data collection. The third part is devoted to relevant applications in food analysis, including validation procedures for screening analysis as well as relevant databases.

Outlines basic concepts and principles of gas and liquid chromatography TOF-MS and its application in food analysisIncludes quality control and data collection techniquesFocuses on environmental implications and safety concerns

In recent years the availability of techniques and the asking of basic and technological questions has led to an international explosion of activity in the study of solid surfaces. Originally published in Reports in Progress in Physics, Electronic Properties of Surfaces reflects the modern knowledge in this field, presenting critical appraisals of progress in surface science. The book should be particularly valuable for researchers new to this field.

The idea for this book arose out of the realization that, although excellent surveys and a phosphor handbook are available, there is no single source covering the area of phosphate based phosphors especially for lamp industry. Moreover, as this field gets only limited attention in most general books on luminescence, there is a clear need for a book in which attention is specifically directed toward this rapidly growing field of solid state lighting and its many applications. This book is aimed at providing a sound introduction to the synthesis and optical characterization of phosphate phosphor for undergraduate and graduate students as well as teachers and researchers. The book provides guidance through the multidisciplinary field of solid state lighting specially phosphate phosphors for beginners, scientists and engineers from universities, research organizations, and especially industry. In order to make it useful for a wide audience, both fundamentals and applications are discussed, together.

Spectroscopic Properties of Inorganic and Organometallic Compounds provides a unique source of information on an important area of chemistry. Divided into sections mainly according to the particular spectroscopic technique used, coverage in each volume includes: NMR (with reference to stereochemistry, dynamic systems, paramagnetic complexes, solid state NMR and Groups 13-18); nuclear quadrupole resonance spectroscopy; vibrational spectroscopy of main group and transition element compounds and coordinated ligands; and electron diffraction. Reflecting the growing volume of published work in this field, researchers will find this Specialist Periodical Report an invaluable source of information on current methods and applications. Specialist Periodical Reports provide systematic and detailed review coverage in major areas of chemical research. Compiled by teams of leading experts in their specialist fields, this series is designed to help the chemistry community keep current with the latest developments in their field. Each volume in the series is published either annually or biennially and is a superb reference point for researchers. www.rsc.org/spr

This thesis represents a breakthrough in our understanding of the noise processes in Microwave Kinetic Inductance Detectors (MKIDs). While the detection of ultraviolet to near-infrared light is useful for a variety of applications from dark matter searches to biological imaging and astronomy, the performance of these detectors often limits the achievable science. The author's work explains the limits on spectral resolution broadening, and uses this knowledge to more than double the world record spectral resolution for an MKID suitable for optical and near-IR astrophysics, with emphasis on developing detectors for exoplanet detection. The techniques developed have implication for phonon control in many different devices, particularly in limiting cosmic ray-induced decoherence in superconducting qubits. In addition, this thesis is highly accessible, with a thorough, pedagogical approach that will benefit generations of students in this area.