In this thesis, the author outlines the development of new monolithic columns and isotope dimethyl labeling strategies and their applications in high-performance proteome analyses. Though different types of monolithic columns have been widely developed for chromatography and electrophoresis separation, their application in proteomics for complex peptide mixtures separation is still a challenge. The author discusses the preparation of new monolithic columns and optimization of chromatography separation capability to improve coverage and accuracy of proteome analysis. Further, the author describes a novel online multidimensional chromatography system combined with automated online isotope labeling, which significantly improves the throughput, sensitivity and accuracy of quantitative proteomics. In addition to the development of new technologies, the author investigates the proteome and phosphoproteome expression changes of clinical hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer s disease. The work in this thesis has led to several publications in high-profile journals in the fields of analytical chemistry and proteome research."

This book provides insight into the underlying basic theories and concepts in X-ray, light, and neutron scattering. The three scattering principles are systematically presented, together with a unified description based on elastic scattering of electromagnetic waves and the Schroedinger wave from matter. These explanations are presented with an introduction of their common Born approximation using a consistent set of symbols and terminology and with step-by-step derivations of equations. This book emphasizes the combined applications of these three scattering methods, wherever and whenever possible, as a very powerful methodology for characterization of internal structures of soft matters in the length scale ranging from subnanometers to a few 10 micron meters. These applications include explorations for evolution of hierarchically self-organized internal structures of a variety of soft matters, including cells, under diverse environmental conditions. This book will not only be an excellent resource for graduate students and academic researchers who analyze structures of soft matters and polymers, but it will also be useful for researchers in industries.

This thesis advances the long-standing challenge of measuring oxidative stress and deciphering its underlying mechanisms, and also outlines the advantages and limitations of existing design strategies. It presents a range of approaches for the chemical synthesis of fluorescent probes that detect reversible changes in cellular oxidative stress. The ability to visualise cellular processes in real-time is crucial to understanding disease development and streamline treatment, and this can be achieved using fluorescent tools that can sense reversible disturbances in cellular environments during pathogenesis. The perturbations in cellular redox state are of particular current interest in medical research, since oxidative stress is implicated in the pathogenesis of a number of diseases. The book investigates different strategies used to achieve ratiometric fluorescence output of the reversible redox probes, which nullify concentration effects associated with intensity-based probes. It also describes suitable approaches to target these probes to specific cellular organelles, thereby enabling medical researchers to visualise sub-cellular oxidative stress levels, and addressing the typically poor uptake of chemical tools into biological studies. In total it reports on four new probes that are now being used by over twenty research groups around the globe, and two of which have been commercialised. The final chapters of this thesis demonstrate successful applications of the sensors in a variety of biological systems ranging from prokaryotes to mammalian cells and whole organisms. The results described clearly indicate the immense value of collaborative, cross-disciplinary research.

Principles and Clinical Diagnostic Applications of Surface-Enhanced Raman Spectroscopy summarizes the principles of surface-enhanced Raman scattering/spectroscopy (SERS) and plasmonic nanomaterials for SERS, with a focus on SERS applications in clinical diagnostics. This book covers the key concepts from the fundamentals, materials, experimental aspects, and applications of SERS in clinical diagnostics with discussions on label-free/direct SERS assay, design and synthesis of SERS nanotags, SERS nanotags for point-of-care diagnostics, microfluidic SERS assay, and in vitro and in vivo sensing and imaging. Written by experts from around the world, this comprehensive volume showcases the recent progress of SERS applications in clinical diagnostics and helps readers understand when and how to use SERS in a clinical setting.

This volume provides up-to-date information on toxic pollutants in the environment and their harmful effects on human health and nature. The book covers many important aspects of environmental toxicology, such as features, characterization, applications, environmental routes for dispersion, nanotoxicity, ecotoxicity and genotoxicity of nanomaterials, with emphasis on radiation toxicology, polar ecotoxicology, plastic toxicology, microbrial toxicology, nanotoxicology and pesticide toxicology. Also discussed is the use of microbes and nanotechnology for medicinal purposes, which has revealed important chemical prototypes in the discovery of new agents, stimulating the use of refined physical techniques and new syntheses of molecules with pharmaceutical applications for human welfare. The chapters also address the fate of nanoparticles in the environment, as well as nanotoxicology mechanisms impacting human health. The book will be of interest to toxicologists, environmental scientists, chemists, and students of microbiology, nanotechnology and pharmacology.

This resource demonstrates how a combination of modern techniques is used to ensure that horseracing is both fair and prevents abuse of the horses involved. Based on the work of the Horseracing Forensic Laboratory (HFL) located near Newmarket in the UK, the book comprises five sections of student material. First, an overview of the work of HFL is presented, followed by sections on immunoassay, metabolism and chromatography. Teachers' notes are also included. Following the explanatory text are questions, which assist with understanding and also illustrate real-life applications of the chemical techniques encountered at school. Chemistry at the Races is designed mostly for ages 16+, but some material is also included for younger students. It is an invaluable resource for teachers, enabling them to demonstrate an up-to-date and interesting context for their work.

This book offers a comprehensive overview of recent studies conducted on the biological effects of metal nanoparticles. It also provides a solid theoretical foundation and various metal nanoparticle synthesis methods. Part I reviews the main chemical methods used for synthesizing metal nanoparticles in a solution and describes original method of biochemical synthesis, as well as some special procedures developed specifically for studying the biological activity of nanoparticles. Part II analyzes current literature on the effects of metal nanoparticles observed in microorganisms and addresses the influence of silver nanoparticles obtained by biochemical synthesis on biological objects on various organization levels, namely on microorganisms, acellular slim mold, unicellular alga, plant seeds and mammalian cells. The last section explains the central problems common in studies on the biological effects of metal nanoparticles and outlines potential uses of this trend in bio-nanotechnologies. This book is aimed at specialists, professors and students aspiring to expand their knowledge about the biological activities of metal nanoparticles and nanoparticle-containing materials.

This book is intended for both the undergraduate and postgraduate levels. The subject material deals with symmetry, starting from the basic and rudimental foundations of the concept and the depth of its applications. The presentation of the concepts and the related illustrations in the book are on semi-pedagogical lines and thus offer easy understanding to the reader. The book is designed with twelve well thought chapters, with each preceding chapter opening the gate for the development of the next thereby having a high degree of sequence.The first seven chapters are devoted to the indepth understanding of 'symmetry and group theory' of molecules and the last five chapters are developed on the aspects of how these formalisms are utilized for the 'structure, spectra and bonding' of molecules. The book also covers the essential basics of the group theory that are required for all sections of chemistry, and emphasizes the necessity of this theory to understand the theoretical and applied aspects of molecular spectroscopy. This text is the result of a long felt need for developing certain novel techniques for the teaching of this course. A 'Window-vision' has been provided in the book while presenting most of the chapters and 'Study Questions' are placed at the end of each chapter to help ensure understanding of the concepts. 'No more nightmares of group theory and spectroscopy' - is the ultimate purpose of this book.

Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products presents applications in diverse areas of both chemical technology and biotechnology. This book serves as a single resource for learning about both the economical, facile and scalable processes, along with their potential for applications in the separation and purification of materials and compounds across the entire spectra of chemical and biological nature. The book begins by explaining the origins and fundamentals of TPP and continues with chapters on related applications, ranging from the purification of parasite recombinant proteases to oil extraction from oilseeds and oleaginous microbes, and more.

The third edition of this book has been thoroughly revised and enlarged to include few chapters pertaining to supercritical fluid chromatography and extraction, electrophoresis, capillary electrophoresis & capillary electro-chromatography, X-Ray spectroscopy, Mossbauer spectroscopy, Raman spectroscopy, electron microscopy, chemiluminescence, atomic fluorescence, ionization spectroscopy, chemical sensors & biological sensors, refractometry & interferometry. In addition, all other chapters have been revised to incorporate new concepts.

Climate Change and Mycotoxins highlights the importance of the continuous study of climate change impacts on mycotoxigenic fungi and their toxins in food and feed crops. Changing climate conditions across every geographical zone greatly affect rainfall, temperature and concentration of greenhouse gases leading to loss in yield and quality of food crops. In outstanding contributions, the authors compile current evidence on the influence of climate change on mycotoxigenic fungi and mycotoxins in food crops pre- and postharvest and during storage of food and animal feed. The chemistry and biology of toxin production is revised and an outlook on control and prevention of the toxin's impact on food and animal feed is given. The editors recommend this book to mycologists, mycotoxicologists, pathologists, epidemiologists, toxicologists, physicians, veterinarians, nutritionists, the food and feed industries, legislators, analytical chemists, microbiologists, or students of these fields. * Unique compilation on the impact of climate change on mycotoxins based on observed trends over the last 10 years. * Special focus on the implications for food and feed safety. * Latest advances on prediction and prevention of mycotoxin threats to human and animal health. About the Editors Luis M. Botana Is a full Professor of Pharmacology at the University of Santiago, from 2004-2012 director of the Department of Pharmacology and former Fogarty Fellow at the School of Medicine of the Johns Hopkins University. He has been director of the European Reference Laboratory for Marine Toxins from 2004 to 2009. He is author of 25 international patents, over 300 scientific papers and editor of 10 international books. Maria J. Sainz Is an associate Professor of Agriculture and Forage Production and Conservation at the University of Santiago de Compostela. She has been a visiting scientist at the Rothamsted Experimental Station and for ten years head of the department of Plant Production. Her research interests focus on fungal pathogen detection and diagnostics, mycorrhizal fungi in crop protection and production, and mycotoxigenic fungi and mycotoxins on forage crops and animal feed.

This volume is ba. sed on the presentations gi ven at the ElectroFinnAnalysis conference held on J une 6-9, 1988 in Turku-Abo, Finland. This event was the second in a series of electroanalytical conferences. The first was held in Ireland 1986 and the next will be held in Spain 1990. The aim of these conferences is tobring tagether scientists who use electroanalytical methods in their research. This is also reflected in the disposition of this volume where instrumentation and applications from the different fields have their own chapters. The editors are grateful to Mr. Johan Nyman, Mr. Kent Westerbolm and Mr. Markku Lehto for their technical assistance during the editorial work of this volume. Ari Ivaska Andrzej Lewenstam Ralf Sara V CONTENTS lntroduction Ari Ivaska ELECTROCHEMICAL INSTRUMENTATION AND METHODS New Instrumental Approaches to Fast Electro-Chemistry at Ultramicroelectrodes . . . . . . . . . . . 5 Larry R. Faulkner, Michael R. Walshand Chuanjing Xu Photoelectroanalytical Chemistry - Methods and Instrumentation . . . 15 J ouko J. Kaukare Experiences of an On-Line Fourier Transform Faradaic Admittance Measurement (FT-FAM) SystemBasedon Digital Signal Processors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Sten 0. Engblom, Mikael Wasberg, Johan Bobacka and Ari Iva. ska Processor-Controlled Fast Potentiostat . '. . . 31 J. Kaukare and J. Lukka. ri Smoothing of AC Polaragraphie Data by FFT Filtering . ' . . . . 37 J oha. n Bobacka. a. nd Ari Jvaska Reverse Pulse Voltammetry at Microelectrodes. New Possibilities in Analytical Chemistry . . . . . . . . . . . . . . . . . . 47 Zbigniew Stojek Multiple Sensor Arrays: Advantages and lmplications 51 Dermot Diamond Simultaneaus ESR-Electrochemical Investigations at Solid Electrodcs . ."

Herbicides: Chemistry, Efficacy, Toxicology, and Environmental Impacts addresses contemporary debates on herbicide toxicology. The reader is offered a comprehensive overview of this complex topic, presented by internationally recognized experts. Information presented will inform discussions on the use of herbicides in modern agricultural and other systems, and their potential non-target effects on human populations and various ecosystems. The book covers these matters in concise language appropriate to engage both specialists in the research community and informed persons responsible for legislative, funding, and public health matters in the community at large. The use of herbicides is an essential pillar of modern agricultural production systems. Weeds, if uncontrolled, would reduce crop yield and result in massive economic damage. Recently, the heavy reliance on single herbicides has been linked to the development of weed resistance. To combat resistant weeds, farmers are advised to use a mix of several herbicides and to increase herbicide application rates. As a result, the toxicity of herbicides on human health and the environment has become a controversial topic.

This book aims to present the different aspects of electrospinning for designing and fabricating high performing materials for sensors applied in gaseous and liquid environments. Since electrospinning is a versatile and inexpensive manufacturing technology, the book emphasizes the industrial applications perspective. The volume is an edited collection of the most recent and encouraging results concerning advanced nanostructured (bio) sensors. The feats achieved by these sensors range from high sensitivity to extreme operating conditions and satisfy a wide range of requirements. Most of the contributions in this book come from First International Workshop on Electrospinning for High Performance Sensing (EHPS2014) that was held in Rome in 2014, as part of the European COST Action MP1206 Electrospun Nanofibres for bio inspired composite materials and innovative industrial applications.

As a spectroscopic method, Nuclear Magnetic Resonance (NMR) has seen spectacular growth over the past two decades, both as a technique and in its applications. Today the applications of NMR span a wide range of scientific disciplines, from physics to biology to medicine. Each volume of Nuclear Magnetic Resonance comprises a combination of annual and biennial reports which together provide comprehensive of the literature on this topic. This Specialist Periodical Report reflects the growing volume of published work involving NMR techniques and applications, in particular NMR of natural macromolecules which is covered in two reports: "NMR of Proteins and Acids" and "NMR of Carbohydrates, Lipids and Membranes." For those wanting to become rapidly acquainted with specific areas of NMR, this title provides unrivalled scope of coverage. Seasoned practitioners of NMR will find this an in valuable source of current methods and applications. Specialist Periodical Reports provide systematic and detailed review coverage in major areas of chemical research. Compiled by teams of leading authorities in the relevant subject areas, the series creates a unique service for the active research chemist, with regular, in-depth accounts of progress in particular fields of chemistry. Subject coverage within different volumes of a given title is similar and publication is on an annual or biennial basis.

Exploring fet Concepts for Lab-on-a-Chip.- Monitoring of Cell Growth, Oxygen and ph in Microfermentors.- Observation of Response to Stimuli in Oscillating Cells Patterned by Microfabricated Structure.- On-Chip Agarose Microchamber (AMC) Array Cell-Cultivation System for Topographical Control of Neural Network.- Integrated Size Exclusion and Reversed-Phase Electrochromatography.- Dispersion Analysis of a Solid-Phase Microextraction Chip by Computational Fluid Dynamics.- Interfacing Microchip CE with Icpms for Element Speciation.- Electrodeless Dielectrophoretic Trapping and Separation of Cells.- SiO2 Nozzle Array-Based Patch-Clamp Microsystem.- A Programmable Cell Assay Platform for Kinetic Studies of a Single Cell.- An Integrated Microdialysis-Based System.- User-Assembly, Fully Integrated Micro Chemical Laboratory using Biochemical IC Chips for Wearable/Implantable Applications.- Microfluidic Reactor Array for Multistep Droplet Reactions.- Single Molecular Detection of DNA/Protein and Its Application to Biochips.- DNA Separation in Gradient of Confined Nanospace Generated by Nanoparticles in a Microchannel.- Self-Assembled Magnetic Colloids for DNA Separations in Microfluidic Devices.- A Microfabricated Device for Separating 200KILO-BASE-PAIR DNA Molecules in 15 Seconds.- Airborn Chemistry Levitated Protein Droplets as a Novel Analytical Tool for Nucleation Screening in Macromolecular Crystallisation.- On-Line Monitoring of Airborne Chemistry in Levitated Droplets: in-SITU Synthesis and Application of Sers Active Ag-Sols For Trace Analysis By Raman Spectrometry.- Chip-To-World Interfaces for High-Throughput Lab-On-A-Chip Devices.- The Mems Modeling System by Collaboration of Multi-Scale Simulators and Application to the Microreactor.- Fem Study of Coulter Counter Wggh Water-Based Adaptable Aperture.- Micromanipulating Magnetic Particles in Microfluidic Systems.- Optimal Design of Micromixer Using Nonhomogeneous Multilayer Laminar Flow.- Computational Simulations of Fluid Flow Dynamics, and Bead Packing in Solid Phase Extraction Microsystems.- The Low-Voltage Cascade EOF Pump: Comparing Theory with Published Data.- Universal Joule Heating Model in Electrophoretic Separation Microchips.- Mathematical Model of Interfacial Layer in Ultra-Fine Liquid Drop Based on Molecular Dynamics Simulation.- Development of Micromixer Based on Instability of Interface Between Two Immiscible Liquids.- Theoretical Study of AC Electroosmotic Flows in Non-Uniformly Charged Microchannels.- Numerical Simulation of Mixing in A Micro-Channel with Non-Uniform Zeta Potential Surface.- Injection of Sample Bands from Open Channels into Packed Separation Columns.- Microscale Measurements of Flow Bounded by Air-Water Interfaces.- Virtual Flow Channel: A Novel Micro-Fluidics System with Orthogonal, Dynamic Control Of Sample Flow Dimensions.- Microfabrication of 3-D Oblique Structures by Inclined UV Lithography.- A New Fabrication Technique of A DNA Electrophoresischip by Silicon Micromachining Technology.- Precision Patterning of PDMS Thin Films: A New Fabrication Method and Its Applications.- Fabrication of Microfluidic Channels with Symmetric Cross-Sections for Integrated NMR Analysis.- Precise and Feasible Fabrication Process of Microfluidic Devices on A Borosilicate Glass Chip.- Laminated Electrodes Chip for Pulse-Immunoassay.- Fabrication of On-Chip Sorter Devices with Sub-Micrometer Scale Channels and Self-Aligned Microelectrodes.- Powder Handling Device for Drug Formulations.- Thermal Micropump Using Surface Tension Imbalance in Microchannels.- Optimization of Electrokinetic Pumps for Chip-Based Chromatographic Separations.- Practical Valves and Pumps for Large-Scale Integration into Microfluidic Analysis Devices.- Electro Osmotic Flow Pump Based on The Corbino Disc Geometry.- A Temperature Controlled Micro Valve for Biomedical Applications Using A Temperature Sensitive Hydrogel.- Electroosmotically Driven Two-Liquid Viscous Pump for Nonconducting...

knowledge. This material provided has been collected from different sources. One important source is the material available from EURACHEM. Eurachem is a network of organisations in Europe having the objective of establishing a system for the international tra- ability of chemical measurements and the promotion of good quality practices. It provides a forum for the discussion of common problems and for developing an informed and considered approach to both technical and policy issues. It provides a focus for analytical chemistry and quality related issues in Europe. You can find more information about EURACHEM on the internet via "Eurachem -A Focus for Analytical Chemistry in Europe" (http: //www.eurachem.org). In particular the site Guides and Documents contains a number of different guides, which might help you to set up a quality system in your laboratory. The importance of quality assurance in analytical chemistry can best be described by the triangles depicted in Figs. 1 and 2. Quality is checked by testing and testing guaranties good quality. Both contribute to progress in QA (product control and quality) and thus to establishing a market share. Market success depends on quality, price, and flexibility. All three of them are interconnected. Before you can analyse anything the sample must be taken by someone. This must be of major concern to any analytical chemist. There is no accurate analysis wi- out proper sampling. For correct sampling you need a clear problem definition. There is no correct sampling without a clear problem definition

This book covers the fundamental aspects of fiber lasers and fiber amplifiers, and includes a wide range of material from laser physics fundamentals to state-of-the-art topics in this rapidly growing field of quantum electronics. This expanded and updated new edition includes substantial new material on nonlinear frequency conversion and Raman fiber lasers and amplifiers, as well as an expanded list of references inclusive of the recent literature in the field. Emphasis is placed on the nonlinear processes taking place in fiber lasers and amplifiers, their similarities, differences to, and their advantages over other solid-state lasers. The reader will learn the basic principles of solid-state physics and optical spectroscopy of laser active centers in fibers, the main operational laser regimes, and will receive practical recommendations and suggestions on fiber laser research, laser applications, and laser product development. The book will be useful for students, researchers, and professional physicists and engineers who work with lasers in the optical and telecommunications field, as well as those in the chemical and biological industries.

Additives have been used in the food sector for centuries, aiming to maintain or improve food quality in terms of freshness, appearance, texture and taste. Most food additives are synthetic chemical compounds classified as antioxidants, antimicrobials, colorants and sweeteners. In the last decades, several synthetic food additives have been correlated with adverse reactions in humans, which has caused the safety of synthetic food additives to be reviewed and discussed by international organizations. At the same time, there is increasing consumer demand for more natural and environmentally friendly food products and additives. Therefore, synthetic food additives have been replaced with natural food additives. Although the use of natural additives is a hot topic in food science, to date no book has systematically reviewed the application of natural additives in food products. Natural additives in foods presents an exhaustive analysis of the most recent advances in the application of natural additives in the food sector. Covering natural antioxidants, antimicrobials, colorants and sweeteners, this text also focuses on unconventional sources of natural additives, valorization and toxicological aspects, consumer attitudes and regulatory aspects. The main applications of natural antioxidants are fully covered, including polyphenols, ascorbic acid, carotenoids, tocopherols and proteins. Natural antimicrobial applications from polyphenols and essential oils to poly-L-Lysine are analyzed, as are natural colorants like anthocyanins, annatto, betalains and paprika. The encapsulation, trapping, and adsorption of natural additives are studied, and consumer perceptions and preferences are major focuses. Researchers will find up-to-date regulatory specifics for the United States and European Union. For any researcher in need of an expansive single source containing all relevant and updated information for the use of natural additives in foods, this book is a much needed addition to the field.

Imaging mass spectrometry (MS) techniques are often utilized without an understanding of their underlying principles, making it difficult for scientists to determine when and how they can exploit MS to visualize their biomolecules of interest. Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry is an essential reference to help scientists determine the status and strategies of biomolecule analysis, describing its many applications for diverse classes of biomolecules. The book builds a foundation of imaging MS knowledge by introducing ionization sources, sample preparation, visualization guidelines, molecule identification, quantification, data analysis, etc. The second section contains chapters focused on case studies on analyzing a biomolecule class of molecules. Case studies include an introduction/background, and a summary of successful imaging MS studies with illustrative figures and future directions.

This volume collects a a number of contributions on spontaneous symmetry breaking. Current studies in this general field are going ahead at a full speed. The book present review chapters which give an overview on the major break throughs of recent years. It covers a number of different physical settings which are introduced when a nonlinearity is added to the underlying symmetric problems and its strength exceeds a certain critical value. The corresponding loss of symmetry, called spontaneous symmetry breaking, alias self-trapping into asymmetric states is extensively discussed in this book.
The book presents both active theoretical studies of spontaneous symmetry breaking effects as well as experimental findings, chiefly for Bose-Einstein-Condensates with the self-repulsive nonlinearity, and also for photorefractive media in optics.

This book presents both updated and new measurements on water resources from selective pilot areas form the Middle East. Demand for water has become very crucial notably in arid and semiarid regions like in the Middle East. The changing climate and the increased population are the most striking challenges on water resources in this region where the largest part of its territory is dry lands and deserts with minimal precipitation and high evaporation rates, and thus, the average per capita of water does not exceed 25 m3/year. This resulted in developing many studies and researches in different Middle East countries to clearly identify and assess the hydrological characteristics of the available water resources and to explore new resources in order to secure balanced water supply/demand. As a first of its type, this book introduces comprehensive knowledge on the successful reports of the use of satellite images in water studies in the Middle East, and the economic value resulted from adopting these techniques. Based on illustrated case-studies the book will represent a significant resource for a large number of experts, academics, researchers, engineers and different-level stakeholders including the decision makers.

Device Architecture and Materials for Organic Light-Emitting Devices focuses on the design of new device and material concepts for organic light-emitting devices, thereby targeting high current densities and an improved control of the triplet concentration. A new light-emitting device architecture, the OLED with field-effect electron transport, is demonstrated. This device is a hybrid between a diode and a field-effect transistor. Compared to conventional OLEDs, the metallic cathode is displaced by one to several micrometers from the light-emitting zone, reducing optical absorption losses. The electrons injected by the cathode accumulate at an organic heterojunction and are transported to the light-emission zone by field-effect. High mobilities for charge carriers are achieved in this way, enabling a high current density and a reduced number of charge carriers in the device. Pulsed excitation experiments show that pulses down to 1 us can be applied to this structure without affecting the light intensity, suggesting that pulsed excitation might be useful to reduce the accumulation of triplets in the device. The combination of all these properties makes the OLED with field-effect electron transport particularly interesting for waveguide devices and future electrically pumped lasers. In addition, triplet-emitter doped organic materials, as well as the use of triplet scavengers in conjugated polymers are investigated.