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Books > Science & Mathematics > Biology, life sciences > Microbiology (non-medical) > Bacteriology
This current book provides an overview of current research on bacteriophages. Chapter One discusses bacteriophages of pathogenic vibrios, identification and differentiation. Chapter Two reviews recent literature about the application of phages for the biocontrol of microorganisms in meat and meat products, with a particular emphasis on chicken, beef and pork in order to shed light on the efficacy of such strategy for the prevention and/or eradication of spoilage and pathogenic microorganisms in foods and food processing environments. Chapter Three studies the synthesis of the divalent cation requirements for efficient adsorption of bacteriophage onto bacterial cells. Chapter Four focuses on the isolation and evaluation of the lytic spectrum of bacteriophages active against food-borne bacteria. Chapter Five presents data about the history and present-day of using bacteriophage preparations in treatment and prevention of various infectious diseases, in particular of suppurative-inflammatory diseases of the respiratory organs. Chapter Six reviews phagebiotics in treatment and prophylaxis of healthcare-associated infections.
This Methods in Molecular Biology (TM) series book presents methods specifically adapted and developed for the study of distinct features of L. pneumophila. Includes materials lists, reproducible protocols, and notes on troubleshooting and pitfalls.
Neisseria meningitidis is a major causative agent, worldwide, of potentially life-threatening meningitis and septicaemia, which carry with them a high mortality rate and permanent physical and neurological issues for survivors. Neisseria meningitidis: Advanced Methods and Protocols offers a collection of methods and protocols that reflect the development and refinement of several new technologies applied to the meningococcus as presented by expert researchers. Several of the chapters in this book describe methods that rely on the collection of complete sets of biological data, for example, using the genome to generate transcriptomes, proteomes, and metabolomes. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neisseria meningitidis: Advanced Methods and Protocols can be readily used to study other pathogens and diseases and should have broad appeal to clinical and non-clinical scientists alike; however, there is still much to learn about the meningococcus and its complex nature, which in turn continues to reveal crucial details about human biology as well.
This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation. The chapters are divided into 4 parts: Part I describes flagellar type III protein exports, assembly, and gene regulation in S. enterica; Part II explains how to isolate the flagella from the bacterial cell bodies, and further explains how to conduct high-resolution structural and functional analyses of the flagellar motor; Part III talks about how to measure flagellar motor rotation over a wide range of external load, how to measure ion motive force across the cytoplasmic membrane, and how to measure dynamic properties of the flagellar motor proteins by fluorescence microscopy with single molecule precision; and Part IV explores the structure and function of Spirochetal, Vibrio, Shewanella, and Magnetococcus flagellar motors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Bacterial Flagellum: Methods and Protocols aims to provide valuable and vital research to aid in the investigation of the bacterial flagellum resulting from various bacterial species.
Microbial biofilms are populations of microorganisms that are found on solid-liquid interface and are typically surrounded by a matrix of extracellular polymeric substances (EPS). The aggregates of cells not firmly attached to a surface are sometimes termed flocs and have the same characteristics as biofilms. Bacteria in the biofilms produce an extracellular matrix and this contributes to ~90% of the biofilm biomass. They are found in all environments such as marine, freshwater, industrial surfaces, domestic appliances exposed to tap water and wastewater, dental surfaces, medical implants etc. This book discusses the characterization, applications and recent advances in the study of biofilms.
William Watson Cheyne (1852-1932), a surgeon by training and a student of Joseph Lister, was a prominent British bacteriologist who published 60 papers and 13 monographs from 1879 to 1927. A proponent of the idea that bacteriology and medicine were interdependent disciplines, he investigated the causes and treatment of wound infections, tuberculosis, cholera, tetanus and gangrene. In 1897, he organized an historical outline of 19th century bacteriology in five landmark periods of discovery, each defined by the work of an influential figure. This study documents his contributions to the history of microbiology and describes his activities as a laboratory investigator, clinician, surgeon, translator, editor and educator.
The present volume by the author is based on the outcome of extensive explorations in the Himalayas for more than a decade. It incorporates the original research findings along with that based on literature survey. It is intended to provide a comprehensive account of an important group of fungi which has a direct bearing on wood industry and forest ecosystem besides commercial application in bioremediation and pollution control. It is the first step in providing the mycologists with consolidated, systematically up-to-date and illustrative monograph of wood-rotting fungi of Himalayas. Every year the students of the post graduate colleges and universities particularly Indian sub-continent go in for fungal forays to collect fungi which forms part of their course curriculum. This book will serve as a field manual for identification. The book has more than 240 color photographs and 123 plates of camera lucida drawings covering all the fungi which have been reported till-to-date from the study area.
Salmonella: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed methods on different aspects and landmarks advancing knowledge on salmonella research. With new chapters on molecular assays for detection, identification and serotyping of salmonella, quantitative proteomic identification of host factors involved in salmonella infection, determination of antimicrobial resistance in salmonella, site-directed mutagenesis, chromosomal gene analysis, development of bacterial nanoparticle vaccine, attachment of nanoparticle cargo to biotinylated salmonella for combination bacteriotherapy against tumors, various microscopy methods to analyze salmonella interaction with host cells, in vitro modeling of gallbladder-associated salmonella colonization, and analysis of salmonella phages and prophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Salmonella: Methods and Protocols, Second Edition present methods that are of value to investigators in the salmonella field.
"The Flagellar World" is a unique publication. The product of years
of research and data collection by the author, this book is a
pictorial guide to flagella in a variety of organisms. Each EM
image is accompanied by a short description of the system in each
organism. These never-before-seen pictures represent a wide variety
of flagella, including "Legionella pneumophila," "Escherichia
coli," "Pseudomonas aeruginosa," and many others. Researchers in
microbiology, immunology, and parasitology will find this a
fascinating and useful resource.
Bacteria belonging to the genus Klebsiella have a dual role in human pathophysiology. Some of the strains are potent opportunistic pathogens capable of causing severe illnesses, whereas a majority of the klebsiellas belong to our normal flora, particularly in our alimentary tract. In this book, the authors present current research in the study of the epidemiology, pathogenesis and clinical outcomes of klebsiella infections. Topics discussed include interactions of klebsiella sp. with other intestinal flora; C-9154 derivatives with potent in vitro biological activity against klebsiella pneumoniae; treatment strategies for infections caused by carbapenem resistance and pandrug resistant klebsiella isolates; clinical characteristics of klebsiella pneumoniae pneumonia in alcoholic patients and virulence factors of the isolates; klebsiella pneumoniae endogenous endophthalmitis; klebsiella pneumoniae liver abscess; virulence mechanisms of the klebsiella; and multi-drug resistant klebsiella pneumoniae and K. oxytoca strains in Russian hospitals.
Recent evidence suggests an increasing rate of antimicrobial resistant pathogens throughout the world. Pathogens like Staphylococcus aureus are showing substantial prevalence of resistance to antibiotics. Thus, we think that given these developments, clinicians would welcome an updated version of this book. A resource indicating appropriate, evidence-based antimicrobial treatment of infectious diseases encountered in both the hospital and outpatient settings would be of significant value to practicing clinicians. The book would focus on the clinical importance of appropriate diagnosis and treatment of infectious diseases particularly in terms of antibiotic-resistance. The resource would be valuable to countless numbers of junior-level practitioners (residents, nurse practitioners, physician-assistants). Moreover, the book could be a resource for generalists as well as infectious disease specialists.
This book presents topical research in the study of E. coli infections, including urinary tract infections caused by E. coli; aptamer-based detection and therapeutics to prevent and treat E. coli infections; Lactoferrin as a novel natural host defence protein; enterotoxigenic escherichia coli colonisation factors and the prevention of E. coli using organic acids.
This handbook gives a comprehensive and copious illustrated description, with original art work, of the most common diseases in laboratory reared insect colonies, comprising Viruses (Baculoviridae, Reoviridae, Poxviridae, Iridoviridae); Bacteria (Bacillaceae, Pseudomonadaceae, Enterobacteriaceae); Rickettsia; Fungi and Microsporidia and Protozoa. Gregarine and Coccidian parasitoids are also depicted. Manuals for the diagnostic of insect diseases have been published in the 90 s, however, these were intended for professional insect pathologists or for proficient training in invertebrate pathology, hence requiring some scientific background in insect pathology. Currently, most guides are web based, not comprehensive and solely addressing the most common diseases in a particular insect, or group of insects, reared in laboratory facilities. A comprehensive and proficient practical handbook for students and technicians working with insect reared colonies is lacking in the current literature.
Bacteria are a large group of unicellular micro-organisms. Most bacteria have a single circular chromosome that can range in size. DNA is organised into chromosomes and, in organisms other than bacteria, it is found only in the cell nucleus. Deoxyribonucleic acid(DNA)is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organisms and some viruses. Their main role is the long-term storage of information. DNA is often compared to a set of blueprints or a code, since it contains the instructions needed to construct other components of cells. This book presents current research on bacterial DNA, DNA polymerase and DNA helicases.
Infectious diseases caused by bacterial pathogens are a leading cause of human illness and death worldwide. The study of bacterial pathogenesis has changed dramatically over the last decade, as a result of revolutionary changes in biotechnology and our understanding of molecular and cellular biological systems. This volume is structured to emphasize paradigms of infectious disease that have emerged over the last 10 years and provides a fundamental understanding of the complex cellular and molecular processes that are important for bacterial virulence and the infectious disease process. The book highlights key techniques and methodologies that have driven recent discoveries in bacterial pathogenesis, major structures and mechanisms in bacteria that are important for their pathogenesis, the regulation of these virulence determinants by global regulators, and bacterial biowarfare agents.
This collection of diverse articles by the pioneers of modern genomics takes stock of the current state of the field and elucidates the contribution that sequencing genomes has made to our understanding of microbial metabolism and evolution. Through twenty-eight thought-provoking chapters, the authors describe some of the most common computational methods and their applications to studying pathogenic microorganisms, show how genomics can be used to reconstruct the history and dynamism of the microbial world, and discuss issues as diverse as reconstruction of metabolic pathways, cell cycle processes, microbial evolution, metagenomics, and vaccine development. Additional chapters deal with microarrays and expression analysis and the role of genomic in drug discovery.
More than eighty years ago, before we knew much about the structure of cells, Russian botanist Boris Kozo-Polyansky brilliantly outlined the concept of symbiogenesis, the symbiotic origin of cells with nuclei. It was a half-century later, only when experimental approaches that Kozo-Polyansky lacked were applied to his hypotheses, that scientists began to accept his view that symbiogenesis could be united with Darwin's concept of natural selection to explain the evolution of life. After decades of neglect, ridicule, and intellectual abuse, Kozo-Polyansky's ideas are now endorsed by virtually all biologists. Kozo-Polyansky's seminal work is presented here for the first time in an outstanding annotated translation, updated with commentaries, references, and modern micrographs of symbiotic phenomena.
Cyanobacteria, also known as blue-green algae, blue-green bacteria or cyanophyta, is a phylum of bacteria that obtain their energy through photosynthesis. They are a significant component of the marine nitrogen cycle and an important primary producer in many areas of the ocean, but are also found in habitats other than the marine environment; in particular, cyanobacteria are known to occur in both freshwater and hypersaline inland lakes. They are found in almost every conceivable environment, from oceans to fresh water to bare rock to soil. Cyanobacteria are the only group of organisms that are able to reduce nitrogen and carbon in aerobic conditions, a fact that may be responsible for their evolutionary and ecological success. Certain cyanobacteria also produce cyanotoxins. This new book presents a broad variety of international research on this important organism.
A bacteriophage is any one of a number of viruses that infect bacteria. The term is commonly used in its shortened form, phage. Typically, bacteriophages consist of an outer protein hull enclosing genetic material. The genetic material can be ssRNA (single stranded RNA), dsRNA, ssDNA, or dsDNA between 5 and 500 kilo base pairs long with either circular or linear arrangement. Bacteriophages are much smaller than the bacteria they destroy - usually between 20 and 200 nm in size. Phages are estimated to be the most widely distributed and diverse entities in the biosphere.[1] Phages are ubiquitous and can be found in all reservoirs populated by bacterial hosts, such as soil or the intestines of animals. One of the densest natural sources for phages and other viruses is sea water, where up to 9x108 virions per millilitre have been found in microbial mats at the surface, and up to 70% of marine bacteria may be infected by phages. They have been used for over 60 years as an alternative to antibiotics in the former Soviet Union and Eastern Europe. They are seen as a possible therapy against multi drug resistant strains of many bacteria. This book presents the latest research in this field from around the world.
Since the discovery of circadian rhythms in cyanobacteria in the late 1980s, the field has exploded with new information. The cyanobacterial model system for studying circadian rhythms, "Synechococcus elongatus," has allowed a detailed genetic dissection of the bacterial clock due to the methods currently available in molecular, structural, and evolutionary biology. This book addresses multiple aspects of bacterial circadian programs: the history and background of the cyanobacteria and circadian rhythms in microorganisms, the molecular basis, structure, and evolution of the circadian clock, entrainment of the oscillator with the environment and the control of downstream processes by the clock, the demonstration of adaptive significance and the prokaryotic clock s remarkable stability, and mathematical and synthetic oscillator models for clock function. Experts in the field provide a timely and comprehensive review and a stepping-stone for future work on this amazing group of microorganisms and timing."
Agrobacterium is a plant pathogen which causes the "crown-gall" disease, a neoplastic growth that results from the transfer of a well-defined DNA segment ("transferred DNA", or "T-DNA") from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades. Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species, from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications. The book is a comprehensive volume describing Agrobacterium's biology, interactions with host species, and uses for genetic engineering.
The critically acclaimed laboratory standard for more than fifty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today truly an essential publication for researchers in all fields of life sciences. This new volume presents methods related to the use of bacterial genetics for genomic engineering. The book includes sections on strain collections and genetic nomenclature; transposons; and phage.
Systematic biology has a far wider application than merely the provision of a reliable classification scheme for new strains. With the framework of the hierarchic system stabilizing, genomes, noncoding regions, and genes and their products can now be evaluated in an evolutionary context. This book summarizes recent developments in the molecular characterization of cultured and as-yet uncultured prokaryotes, emphasizing the strengths and weaknesses of individual approaches. The chapters of the book are compiled to stimulate students to enter the field of bacterial diversity, presenting a broad spectrum of fascinating multifaceted disciplines that illuminate the paths to ecosystem functioning, communication within communities, symbiosis, life in extreme environments, astrobiology, and more.
The book summarizes the achievements of the past decade in the biochemistry, bioenergetics, structural and molecular biology of respiratory processes in selected genera of the domain Bacteria along with an extensive coverage of the redox chains of extremophiles belonging to the Archaean domain. The volume is a unique piece of work since it contains a series of chapters dealing with metabolic features having important microbiological and ecological relevance such as the use of ammonium, iron, methane, sulfur and hydrogen as respiratory substrates or nitrous compounds in denitrification processes. Particular attention is also dedicated to peculiar groups of prokaryotes such as Gram positives, acetic acid bacteria, pathogens of the genera Helicobacter and Campylobacter, nitrogen fixing symbionts and free-living species, oxygenic phototrophs (Cyanobacteria) and anoxygenic (purple non-sulfur) phototrophs. The book is intended to be a long-term source of information for Ph.D. students, researchers and undergraduates from disciplines such as microbiology, biochemistry and ecology, studying basic and applied sciences, medicine and agriculture.
A lethal germ is unleashed in the U.S. mail. A chain of letters spreads terror from Florida to Washington, D.C., from New York to Connecticut, from the halls of Congress to the assembly lines of the U.S. Postal Service. Five people die, and ten thousand more line up for antibiotics to protect against exposure. The government, already outsmarted by the terrorist hijackers of 9/11, leaves its workers vulnerable and a diabolical killer on the loose. Based on hundreds of hours of interviews and a review of thousands of pages of government documents, "The Killer Strain is the definitive account of the year in which bioterrorism became a reality in the United States. Revealing the little-known victims and unsung heroes in the anthrax debacle, investigative reporter Marilyn Thompson also examines the FBI's slow-paced investigation of the crimes and the unprecedented scientific challenges posed by the case. "The Killer Strain, more than just a thrilling read, is also a clarion wake-up call. It shows how billions of dollars and a decade of elaborate bioterror dress rehearsals meant nothing in the face of a real attack -- and how we may still be at risk. |
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