The volume discusses novel issues associated with the neurotoxicity of select metals

mRNA 3' End Processing and Stability, Volume 655 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics. Each chapter is written by an international board of authors.

Viral Replication Enzymes and their Inhibitors Part A, Volume 49, the latest release in the Enzymes series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of related topics.

Coenzyme B12 Enzymes, Part B, Volume 169 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on Structural characterization of cobalamin-dependent radical SAM methylases, Purification and characterization of sequential cobalamin-dependent radical SAM methylases ThnK and TokK in -lactam antibiotic biosynthesis, Characterization of the cobalamin-dependent radical S-adenosyl-L-methionine enzyme C-methyltransferase Fom3 in fosfomycin biosynthesis, Studies of OxsB and GenK, two B12-dependent radical SAM enzymes involved in natural product biosynthesis, Purification and structural elucidation of the cobalamin-dependent radical SAM enzyme OxsB, and more. Other chapters discuss Methods for studying the mechanisms of B12 enzymes, Computational investigations of B12 dependent enzymatic reactions, Using kinetic isotope effects to probe the mechanisms of adenosylcobalamin-dependent enzymes, Steady-state and pre-steady state kinetic analysis of ornithine 4,5-aminomutase, and more.

The objective of this book is to bring into focus the practical application of the theoretical knowledge of crop physiology. This book on Crop Physiology describes basic principles and procedures with exercises dealing with demonstration of important physiological and metabolic processes viz. osmosis, diffusion, water potential, stomatal frequency and index, plant water status, water relation, Measurement of absorption spectrum of chloroplastic pigments and fluorescence, leaf area, plant growth and transpiration. Demonstration of photosynthesis, transpiration, relationship between absorption and transpiration, conditions for seed germination, seed viability and dormancy aspects, chemical solution preparation for physiological and biochemical analysis of plant samples.

Carotenoids: Carotenoid and Apocarotenoid Biosynthesis, Metabolic Engineering and Synthetic Biology, Volume 671, the latest release in the Methods of Enzymology series highlights new advances in the field with chapters on Metabolomics-based analysis of carotenoids and related metabolites in various species via quantitative trait loci and genome wide association mapping approaches, Using bacteria for functional analysis of genes encoding carotenoid biosynthetic enzymes, Rice Callus as a High Throughput Platform for Synthetic Biology and Metabolic Engineering of Carotenoids, Transient expression in Nicotiana benthamiana: A simple platform to investigate genes encoding carotenoid biosynthesis enzymes from diverse algal lineages, and much more. Additional chapters in this new release cover Protein-protein interaction techniques to investigate post-translational regulation of carotenogenesis, The isolation of sub-chromoplast structures from tomato and capsicum fruit, Carrot protoplasts as a suitable method for protein subcellular localization, High throughput production and characterization of carotenoid enzymes for structural and functional studies, Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation, and more.

Scientists are deciphering the biology of the tumor cell at a level of detail that would have been hard to imagine just a decade or so ago. The development of high-throughput DNA sequencing and genomics technologies have allowed an understanding of the development, growth, survival, and spread of cancer cells in the body. From this information, we now have a basic blueprint or roadmap of how a single damaged cell can develop into a pre-malignant lesion, a primary tumor, and finally, a lethal tumor that may spread throughout the body and resist both medical therapy and host immune responses. In this book, we provide an overview of our current understanding of this cancer blueprint, which has been aided both by the study of familial cancer syndromes, in vitro studies of cancer cells, and animal models. Three classes of genes have emerged from these studies: tumor suppressor genes needed for normal growth control and DNA repair; oncogenes that regulate cell growth and survival, and epigenetic modifiers, enzymes that regulate the modification of DNA and the proteins that form chromatin. Each of these three classes of genes is mutated or altered at least once in virtually all malignant cancer cells. Current technologies permit the DNA sequencing of cancer exomes (coding gene sequencing), whole genomes, transcriptome (all expressed genes), and DNA methylation profiling. These studies show that all tumors have unique constellations of mutated, rearranged, amplified, and deleted genes. Single-cell sequencing further shows that there is extensive variation in individual cells in the tumor; that cancers evolve, and have many of the properties of a multi-cellular entity. Lastly, cancer cells, through mutations in epigenetic modifiers, can reprogram the genome and unlock entire developmental and gene expression pathways to adapt and survive in changing conditions. This reprogramming allows the tumor to elude the host body's defenses, radiotherapy, chemotherapy, and targeted therapy that we use in cancer treatment. Understanding this cancer blueprint paves the way for the development of future therapies to treat and eliminate cancer.

Feed additives are non-nutritive substances, preparations and micro-organisms that are added to feed to animal feeds to improve growth performance, feed intake and the efficiency of feed utilization for healthy, economic and eco-friendly livestock production. This book on Advances in Animal Feed Additives addresses current information on the use of different animal feed additives with regard to production, health and reproduction of livestock and poultry. This book contains twenty five s contributed by 30 eminent scientists of animal nutrition, which highlights the significance of antioxidants, enzymes, probiotics, prebiotics, synbiotics, antimicrobials, organic acids, coccidiostats, mycotoxin binders, immunomodulator, hen egg antibody, hormones, beta agonist, methane inhibitors, defaunating agents, essential oil and herbal feed additives. etc. for sustainable livestock and poultry production. Each of the book attempts at providing clear and updated information on feed additives supported with good amount of the experimental evidence and references which will enable the students and research workers to obtain information quickly when necessary. The book is useful to students of animal sciences, teachers and scientists of animal nutrition discipline, personnel of feed industry, field veterinarians, animal husbandry extension workers and progressive animal farmers.

Immunology is the study of immunity or immune system, the system which is involved in the protection of the body from various types of infections. When a foreign substance invades the body, the body evokes various mechanisms to protect itself. To comprehend the subject like immunology, it is essential to study it in its all aspects. This book is a unique approach to understand the fundamentals and emerging trends of immunology in a very simple and self-explanatory form. The book is divided into twenty s covering all important areas of immunology which are essential for proper understanding of the subject. Besides covering fundamental concepts of immunology, there is a separate on Immunotechniques which are commonly used in laboratories. A comprehensive Glossary of important terms has also been included in the book for quick reference.

This book will serve the long standing need of the students, teachers, research workers and other stake holders. The book will be helpful to the students and researchers in developing basic understanding of Animal Welfare sciences.

Thermodynamics is fundamental to university and college curricula in chemistry, physics, engineering and many life sciences around the world. It is also notoriously difficult for students to understand, learn and apply. What makes this book different, and special, is the clarity of the text. The writing style is fluid, natural and lucid, and everything is explained in a logical and transparent manner. Thermodynamics is a deep, and important, branch of science, and this book does not make it "easy". But it does make it intelligible. This book introduces a new, 'Fourth Law' of Thermodynamics' based on the notion of Gibbs free energy, which underpins almost every application of thermodynamics and which the authors claim is worthy of recognition as a 'law'. The last four chapters bring thermodynamics into the twenty-first century, dealing with bioenergetics (how living systems capture and use free energy), macromolecule assembly (how proteins fold), and macromolecular aggregation (how, for example, virus capsids assemble). This is of great current relevance to students of biochemistry, biochemical engineering and pharmacy, and is covered in very few other texts on thermodynamics. The book also contains many novel and effective examples, such as the explanation of why friction is irreversible, the proof of the depression of the freezing point, and the explanation of the biochemical standard state.