Ion Transport and Membrane Interactions in Vascular Health and Disease, Volume 90 in the Current Topics in Membranes series, highlights advances in the field, with this new volume including chapters on an Introduction to Ion transport and membrane interactions in vascular health and disease, Endothelial cell microdomains of Ca2+ and metabolism, Cytoplasmic nanojunctions between smooth muscle organelles control specific Ca2+ signaling, TRPML channels in coronary vascular phenotypic modulation and calcification, Local voltage-gated Ca2+ channel regulation of vascular smooth muscle in diabetes, Biphasic changes in smooth muscle Ca2+ transporters during the progression of coronary atherosclerosis, Role of K+ channels in atherosclerosis, and more.

Human Microbiome in Health and Disease, Volume 192, Part B includes chapters surrounding the role of human microbiome in different diseases. Chapters in this comprehensive new volume include The microbiome and communicable diseases, Gut Microbiome and Antimicrobial Resistance in bacterial pathogens, Dysbiosis of human microbiome and infectious disease, Gastrointestinal microbiome in the context of infection in stomach and gastroduodenal diseases, Respiratory tract microbiome and pneumonia, Gut microbiome and neonatal sepsis, Diarrheal disease and gut microbiome, The microbiome and non-communicable diseases, Gut microbiome and inflammatory bowel disease, Gut microbiome and undernutrition, Human microbiome and cardiovascular disease, and much more.

Addition, Elimination and Substitution: Markovnikov, Hofmann, Zaitsev and Walden: Discovery and Development discusses foundational reactions in organic chemistry and their major protagonists, contributions to synthesis, and history. Hofmann, Zaitsev, and Markovnikov are introduced, along with their major discoveries and contributions to organic chemistry. The history of controversies around Markovnikov's Rule are addressed. The book introduces Walden's original demonstration of configuration inversion, then discusses bimolecular elimination reactions, regioselective addition reactions, regiospecific alkene synthesis, and the development of modern reactions with configuration inversion. With its unique perspective, focus, and comprehensive coverage, this book belongs on the shelf of every organic chemist.

Role of TRPV4 Channels in Different Organ Systems, Volume 89, the latest release in the Current Topics in Membranes series, provides an in-depth description of the role of TRPV4 ion channels in different organ systems under healthy and disease conditions. Research over the last two decades shows crucial physiological and pathological roles for TRPV4 channels. In this volume, we present chapters focusing on TRPV4 channels in the vasculature, lungs, heart, kidney, bladder, skin, musculoskeletal system, eye, and immune cells. Understanding the behavior of TRPV4 channels in various organ systems will provide a big picture on their physiological roles and therapeutic targeting.

Advances in Microbial Physiology, Volume 81 highlights new advances in the field with this new release presenting interesting chapters written by an international board of authors. Updates in this release include sections on Antibiotic tolerance, Lanthanides in bacterial proteins, Bacterial toxins and host-microbe interactions, and Nitric oxide.

DNA Damage and Double Strand Breaks, Volume 51 in The Enzymes series, highlights new advances in the field, with this new volume presenting interesting chapters that provide an update on female and male genital schistosomiasis and a call to integrate efforts to escalate diagnosis, treatment and awareness in endemic and non-endemic settings, vertebrates as uninfected disseminators of helminth eggs and larvae, and combatting anthelmintic resistance in ruminants.

Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as DNA and protein engineering to create protein bioswitches with new functions, Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases, An experimental protocol to study lipid transfer proteins, Synthesis of small heat shock proteins, Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins, An experimental protocol for glycoconjugate analysis, Methods for proximity-based biotinylation combined with Mass Spectrometry, and more. Additional chapters cover Synthetic antibody fragments as conformational sensors of protein activation and trafficking, Expression, purification, functional analysis and crystallization of Rag GTPase, Purification of bacterial transcription elongation complexes by photoreversible immobilization, Inhibition of c-Myc-MAX heterodimerization, Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases, and much more.

Carotenoids: Carotenoid and Apocarotenoid Biosynthesis, Metabolic Engineering and Synthetic Biology, Volume 671, the latest release in the Methods of Enzymology series highlights new advances in the field with chapters on Metabolomics-based analysis of carotenoids and related metabolites in various species via quantitative trait loci and genome wide association mapping approaches, Using bacteria for functional analysis of genes encoding carotenoid biosynthetic enzymes, Rice Callus as a High Throughput Platform for Synthetic Biology and Metabolic Engineering of Carotenoids, Transient expression in Nicotiana benthamiana: A simple platform to investigate genes encoding carotenoid biosynthesis enzymes from diverse algal lineages, and much more. Additional chapters in this new release cover Protein-protein interaction techniques to investigate post-translational regulation of carotenogenesis, The isolation of sub-chromoplast structures from tomato and capsicum fruit, Carrot protoplasts as a suitable method for protein subcellular localization, High throughput production and characterization of carotenoid enzymes for structural and functional studies, Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation, and more.

Occupational Neurotoxicology, Volume Seven covers neurotoxicants and exposures to a variety of hazards in the workplace and how they can affect nervous functions in different ways. Metals and organic compounds, both at high level acute exposure and long-term low-level exposure can affect motor functions, cognition, behavior and neurosensory functions. Sections cover Oil leakage in aircraft, aerotoxic syndrome, Organic solvents, WTC neurotoxicities, Environmental intolerance, Pesticides, Carbon monoxide, Mercury Neurotoxicity in Gold Miners, and the Current evolution of neurobehavioral methods.

The Chlamydomonas Sourcebook, Third Edition, Volume Two: Organellar and Metabolic Processes has been fully revised and updated to include a wealth of new resources for the Chlamydomonas community. Long known for its comprehensive insights into this highly multidisciplinary topic, this edition includes new views on Chlamydomonas branched electron transfer pathways in photosynthesis, the organization of pyrenoids and CO2 concentration mechanisms, and the fermentative-aspects of dark metabolism. Further, the book covers Chlamydomonas and its use in microalgae biotechnology. In addition to updates to previous editions, this book presents the latest in research and best practices, thus making it a must-have resource for those working with the Chlamydomonas organism.

Protein Interaction Networks, Volume 131 in the Advances in Protein Chemistry and Structural Biology series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors.

Advances in Biomembranes and Lipid Self-Assembly, Volume 35, formerly titled Advances in Planar Lipid Bilayers and Liposomes, provides a global platform for the study of cell membranes, lipid model membranes and lipid self-assemblies, from the micro- to the nanoscale. As planar lipid bilayers are widely studied due to their ubiquity in nature, this book presents research on their application in the formulation of biomimetic model membranes, and in the design of artificial dispersion of liposomes. Chapters cover Physical properties of SOPC lipid membranes containing cholesterol by molecular dynamics simulation, Exciting membrane fluctuations - more than thermal stimulation, Fluctuations shaping bio-membrane adhesion, and more.

Advances in Clinical Chemistry, Volume 108, the latest installment in this internationally acclaimed series, contains chapters authored by world-renowned clinical laboratory scientists, physicians and research scientists. The serial discusses the latest and most up-to-date technologies related to the field of clinical chemistry, with this new release focusing on Nephrinuria and podocytopathies, Extracellular vesicles in respiratory disease, Diabetes and cognitive decline, Testosterone analysis in prostate cancer, Mono/polyclonal free light chains as challenging biomarkers for immunological abnormalities, and more.

G Protein-Coupled Receptors, Part A, Volume 193 in the Progress in Molecular Biology and Translational Science series, highlights new advances in the field. G protein-coupled receptors (GPCRs) represent the largest class of cell surface proteins with an intricate involvement of nearly every physiological process in our body. Approximately, one third of the clinically prescribed medicines target GPCRs and the quest to improve the exiting GPCR-targeting therapeutics continues. These two volumes are focused on activation, signaling and regulation of GPCRs with specific examples of receptor systems and cellular processes.

Coenzyme B12 Enzymes, Part B, Volume 169 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on Structural characterization of cobalamin-dependent radical SAM methylases, Purification and characterization of sequential cobalamin-dependent radical SAM methylases ThnK and TokK in -lactam antibiotic biosynthesis, Characterization of the cobalamin-dependent radical S-adenosyl-L-methionine enzyme C-methyltransferase Fom3 in fosfomycin biosynthesis, Studies of OxsB and GenK, two B12-dependent radical SAM enzymes involved in natural product biosynthesis, Purification and structural elucidation of the cobalamin-dependent radical SAM enzyme OxsB, and more. Other chapters discuss Methods for studying the mechanisms of B12 enzymes, Computational investigations of B12 dependent enzymatic reactions, Using kinetic isotope effects to probe the mechanisms of adenosylcobalamin-dependent enzymes, Steady-state and pre-steady state kinetic analysis of ornithine 4,5-aminomutase, and more.

Environmental pollution as a consequence of diverse human activities has become a global concern. Urbanization, mining, industrial revolution, burning of fossil fuels/firewood and poor agricultural practices, in addition to improper dumping of waste products, are largely responsible for the undesirable change in the environment composition. Environmental pollution is mainly classified as air pollution, water pollution, land pollution, noise pollution, thermal pollution, light pollution, and plastic pollution. Nowadays, it has been realized that with the increasing environmental pollution, impurities may accumulate in plants, which are required for basic human uses such as for food, clothing, medicine, and so on. Environmental pollution has tremendous impacts on phenological events, structural patterns, physiological phenomena, biochemical status, and the cellular and molecular features of plants. Exposure to environmental pollution induces acute or chronic injury depending on the pollutant concentration, exposure duration, season and plant species. Moreover, the global rise of greenhouse gases such as carbon monoxide, carbon dioxide, nitrous oxides, methane, chlorofluorocarbons and ozone in the atmosphere is among the major threats to the biodiversity. They have also shown visible impacts on life cycles and distribution of various plant species. Anthropogenic activities, including the fossil-fuel combustion in particular, are responsible for steady increases in the atmospheric greenhouse gases concentrations. This phenomenon accelerates the global heating. Studies have suggested that the changes in carbon dioxide concentrations, rainfall and temperature have greatly influenced the plant physiological and metabolic activities including the formation of biologically active ingredients. Taken together, plants interact with pollutants, and cause adverse ecological and economic outcomes. Therefore, plant response to pollutants requires more investigation in terms of damage detection, adaptation, tolerance, and the physiological and molecular responses. The complex interplay among other emerging pollutants, namely, radioisotopes, cell-phone radiation, nanoparticles, nanocomposites, heavy metals etc. and their impact on plant adaptation strategies, and possibility to recover, mitigation, phytoremediation, etc., also needs to be explored. Further, it is necessary to elucidate better the process of the pollutant's uptake by plant and accumulation in the food chain, and the plant resistance capability against the various kinds of environmental pollutants. In this context, the identification of tolerance mechanisms in plants against pollutants can help in developing eco-friendly technologies, which requires molecular approaches to increase plant tolerance to pollutants, such as plant transformation and genetic modifications. Pollutant-induced overproduction of reactive oxygen species that cause DNA damage and apoptosis-related alterations, has also been examined. They also trigger changes at the levels of transcriptome, proteome, and metabolome, which has been discussed in this book.

Large Animals as Models for Human Diseases, Volume 189 in the Progress in Molecular Biology and Translational Science series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of important topics, including Sheep as a model for neuroendocrinology research, Relevance of dog as a model for urologic diseases, Relevance of lactocrine hypothesis to human maternal programming of development, Comparative aspects of embryo implantation, Pig as an animal model for obesity, Canine models of cancer, Bovine model for human ovarian diseases, Mutations in G protein-coupled receptors in large animals: Modeling human diseases, and more.

B12 Enzyme, Volume 667 in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting timely, interesting chapters, including Part I: B12 Biosynthesis and B12 Chaperones, Probing the preference and regioselectivity of lower ligand activation and its role in cobamide diversity, Biosynthesis of corrinoids, The human B12 trafficking chaperones: CblC, CblD and ATR, A method for the efficient adenosylation of corrinoids, Production, purification and liposome reconstitution of cobamide synthase, Extraction and Purification of Cobamides from Bacterial Cultures, Part II: The many faces of B12-dependent enzyme reactions, Methylcobalamin-dependent methionine synthase, and much more.

Carotenoids: Carotenoid and Apocarotenoid Analysis, Volume 670, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume covering Getting to know carotenoids, Laser capture of tissues for micro-scale carotenoid analyses, Metabolic engineering of carotenoids: procedures for metabolomic characterization, LC-MS analysis of intracellular metabolites for precursors to the carotenoid pathway, Use of E. coli to produce carotenoid standards, HPLC analysis of carotenoids from Bacteria, Purification and development of standards for carotenoid quantification in plant tissues, and much more. Additional sections in this release cover Ultra-High Performance Liquid Chromatography-Mass Spectrometry Analysis of Plant Apocarotenoids, Detection and analysis of novel and known volatile plant apocarotenoids, Carotenoid extraction, detection, and analysis in citrus, Strategies For The Separation And Tentative Identification Of Geometrical (Cis/Trans, Z/E) Isomers Of Carotenoids, Use of stable isotopes to study bioconversion and bioefficacy of pro-vitamin A carotenoids, Carotenoid extraction and analysis of blood plasma/serum, and more.

Bioinformatics in Agriculture: Next Generation Sequencing Era is a comprehensive volume presenting an integrated research and development approach to the practical application of genomics to improve agricultural crops. Exploring both the theoretical and applied aspects of computational biology, and focusing on the innovation processes, the book highlights the increased productivity of a translational approach. Presented in four sections and including insights from experts from around the world, the book includes: Section I: Bioinformatics and Next Generation Sequencing Technologies; Section II: Omics Application; Section III: Data mining and Markers Discovery; Section IV: Artificial Intelligence and Agribots. Bioinformatics in Agriculture: Next Generation Sequencing Era explores deep sequencing, NGS, genomic, transcriptome analysis and multiplexing, highlighting practices forreducing time, cost, and effort for the analysis of gene as they are pooled, and sequenced. Readers will gain real-world information on computational biology, genomics, applied data mining, machine learning, and artificial intelligence. This book serves as a complete package for advanced undergraduate students, researchers, and scientists with an interest in bioinformatics.

Pseudokinases, Volume 667, the latest release in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters, including the Production and Purification of the PEAK pseudokinases for structural and functional studies, Structural biology and biophysical characterization of Tribbles pseudokinases, Detecting endogenous TRIB protein expression and its downstream signaling, Analysis of human Tribbles 2 pseudokinase, Expression, purification and examination of ligand-binding to IRAK pseudokinases, Characterization of pseudokinase ILK-mediated actin assembly, Biochemical examination of Titin pseudokinase, Approaches to study pseudokinase conformations, CRISPR editing cell lines for reconstitution studies of pseudokinase function, and much more.

Advances in Biomolecular EPR, Volume 666 in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics including Magnetic Resonance Characterization of Physiologically Important Metal Ion Binding Sites in the Prion and Related Proteins, The catalytic role of metal-radical/protein-based radicals in heme enzymes, Rigid Cu2+-based spin labels for the study of higher-order DNA G-quadruplex structures, Orthogonal spin labeling and membrane proteins: increasing the information content and going towards in cell applications, Spectroscopic investigation of mono- and di-Mn-containing centers in biochemistry with an emphasis on application of paramagnetic resonance techniques, and more. Additional chapters cover In Vivo pO2 Imaging of Tumors: Oxymetry with Very Low-Frequency Electron Paramagnetic Resonance, an Update, EPR contributions to understanding molybdenum-containing enzymes, EPR spectroscopy of Type I reaction centers, Characterization of a substrate-derived radical in the NosN reaction during the biosynthesis of nosiheptide, and much more.

Protein Design and Structure, Volume 130 in the Advances in Protein Chemistry and Structural Biology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

Advances in Microbial Physiology, Volume 150 in this important serial, highlights new advances in the field with this new volume including content by an international board of authors. Chapters in this new release include Organization of respiratory chains in the bacterial cell, Anaerobic methane oxidizing archaea, Dawn of the DedA: the structure and function of the DedA family of integral membrane proteins associated with bacterial viability and antimicrobial resistance, Nickel, an essential virulence determinant of Helicobacter pylori: trafficking pathways and their targeting by bismuth, Dissimilatory sulfur compounds oxidation in thermophilic and chemolithoautotrophic bacteria belonging to the Aquificales order, and much more.