Enzymes Conjugated to Graphene, Volume 609 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on Enzyme immobilization, Detection of Urea, Enzyme immobilization Enzyme immobilization, PAMAM dendrimer modified reduced graphene oxide post functionalized by horseradish peroxidase for biosensing H2O2, HRP immobilized for LEV detection, Enzyme immobilization, Graphene biocatalysts, Enzyme immobilization, Interactions, Enzyme immobilization, GQD, Enzyme Immobilization, and Enzyme immobilization on functionalized graphene oxide nanosheets.

Cell Volume Regulation and Fluid Secretion, Volume 81, the latest release in the Current Topics in Membranes series highlights new advances in the field, with this new volume presenting interesting chapters on General principles of cell volume regulation, Cell volume maintenance: water and salt homeostasis, The search for ubiquitous cell volume sensor: the role of plasma membrane and cytoplasmic hydrogel, More than membranes, Cellular and membrane biomechanics of CVR response, Molecular identities of volume-regulatory anion channels, Molecular biology and physiology of volume-regulated anion channel (VRAC), the Role of WNKs in the modulation of intracellular chloride, amongst other topics.

Advances in Rice Research for Abiotic Stress Tolerance provides an important guide to recognizing, assessing and addressing the broad range of environmental factors that can inhibit rice yield. As a staple food for nearly half of the world's population, and in light of projected population growth, improving and increasing rice yield is imperative. This book presents current research on abiotic stresses including extreme temperature variance, drought, hypoxia, salinity, heavy metal, nutrient deficiency and toxicity stresses. Going further, it identifies a variety of approaches to alleviate the damaging effects and improving the stress tolerance of rice. Advances in Rice Research for Abiotic Stress Tolerance provides an important reference for those ensuring optimal yields from this globally important food crop.

New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Genes Biochemistry and Applications consolidates the most widely used genetic methods available, bringing together the fields of biochemistry, biotechnology, and microbiology. The chapters outlined give clear and concise direction on both standard and applied microbial genetic improvements, presenting undergraduates, postgraduates, and researchers with the latest developments in microbial gene technology. In addition, the book describes the background and usefulness of each experiment in question. All chapters covered in the book are derived from current peer-reviewed literature as accepted by the international scientific community.

Chemical and Biochemical Approaches for the Study of Anesthetic Function, Part B, Volume 603, presents a coherent description of the campaign towards understanding anesthesia. It includes a variety of highly debated topics, including sections on computational approaches, best practices for simulating ligand-gated ion channels interacting with general anesthetics, computational approaches for studying voltage-gated ion channels modulation by general anesthetics, anesthetic parameterization, the kinetic modeling of electrophysiology data, evolving biophysical technologies, fluorescent anesthetics, lipids, membranes and pressure reversal, in vivo technologies, and more.

Lipid Modification by Enzymes and Engineered Microbes covers the state-of-the art use of enzymes as natural biocatalysts to modify oils, also presenting how microorganisms, such as yeast, can be designed. In the past ten years, the field has made enormous progress, not only with respect to the tools developed for the development of designer enzymes, but also in the metabolic engineering of microbes, the discovery of novel enzyme activities, and in reaction engineering/process development. For the first time, these advances are covered in a single-volume that is edited by leading enzymatic scientist Uwe Borchscheuer and authored by an international team of experts.

Sirtuins in Health and Disease, Volume 154 presents the reactions catalyzed by sirtuins in terms of their unique coenzyme NAD+-dependent catalytic mechanisms, the ways to elucidate these mechanisms, and the design of the inhibitory compounds against the sirtuin-catalyzed reactions as potential therapeutic agents for human diseases. Sections cover the concepts and chemical tools in sirtuin research, SIRT4 and SIRT7, the regulatory effects of NAD metabolic pathways on sirtuin activity, sirtuins as modifiers of Huntington's Disease (HD) pathology, parasite sirtuins as targets for novel chemotherapeutic agents, targeting sirtuins - substrate specificity and inhibitor design, and chemical probes in sirtuin research. This book focuses on the outstanding issues in the sirtuin field and implications for future sirtuin research.

Protein-Protein Interactions in Human Disease, Part A, Volume 110 aims to promote further research and development in the protein interaction network as a means to not only identify the critical proteins involved in the etiology of human diseases, but also identify new protein targets for drug development. Sections cover such topics as protein-protein interaction modulators for epigenetic therapies, intrinsic disorder, protein-protein interactions and disease, targeting protein-protein interactions in the ubiquitin-proteasome pathway, the proteomics of occupational diseases, and computational methods in predicting the impact of SNPs in protein-protein network, amongst other topics.

Advances in Clinical Chemistry, Volume 83, the latest installment in this internationally acclaimed series, contains chapters authored by world-renowned clinical laboratory scientists, physicians and research scientists. The serial discusses the latest, most up-to-date technologies related to the field of clinical chemistry, with this release presenting comprehensive updates on Urinary Nucleosides and Deoxynucleosides, Locked Nucleic Acid Technology for Highly Sensitive Detection of Somatic Mutations in Cancer, Liquid Biopsy: From Basic Research to Clinical Practice, Circulating Tumor Cells and Implications of the Epithelial-to-Mesenchymal Transition, and more.

Therapeutic Proteins and Peptides, Volume 112 in an ongoing series promotes further research in the discovery of new therapeutic targets that can be affected by therapeutic proteins and peptides to cure or manage symptoms of human diseases, with this release focusing on the Rational Design of Stable Liquid Formulations of Biopharmaceuticals, Formulation strategies for peptides, proteins and antibodies using nanotechnology, the Solution structural dynamics of therapeutic peptides and their adsorption on plasmonic nanoparticles, Enzymatic approaches of protein-polymer conjugation, Chimeric small antibody fragments as a strategy to deliver therapeutic payloads, Smart cell-penetrating peptide-based techniques for cytoplasmic delivery of therapeutic macromolecules, and more.

The identification and quantification of the widespread occurrence of trace organic chemicals at minute concentrations in the aqueous environment impacted by human activities is a result of rapid advances in environmental analytical chemistry. The body of knowledge regarding the characterization, fate and transport of these chemicals of emerging concern (CECs) in the natural water environment and engineered water treatment processes, as well as their toxicity, has grown substantially over the last two decades. Recently, the focus in the environmental chemistry community has shifted from these CEC parent compounds to the fate, transport, and toxicity of transformation products, which are generated through abiotic and biotic mechanisms in natural systems and during engineered advanced water treatment processes. This two-part book focuses on the studies and recent advancements towards the development of more harmonized strategies and workflows using non-target and suspects screening methods, including suitable bioassay approaches to assess the overall relevance of transformation products. Volume I covers the relevance of transformation products and international strategies to manage CECs, new methods for a comprehensive assessment of transformation products, and the fate and transport of transformation products in natural systems. This book is ideal for environmental scientists and engineers, particularly chemists, environmental engineers, public health officials, regulators, other chemistry-related professionals, and students.

WNT Signaling, Volume 153, the latest release in the Progress in Molecular Biology and Translational Science series, seeks to provide the most topical, informative, and exciting monographs available on a wide variety of research topics related to prions, viruses, bacteria and eukaryotes. The series provides readers with in-depth knowledge of important molecular biological aspects of organismal physiology and function, along with insights into how this knowledge may be applied to understand and ameliorate human disease. Each volume is edited by an internationally recognized expert who selects contributors at the forefront of a field.

Hematopoietic Stem Cell Niche focuses on an individual organ, looking at the stem cells in the organ itself (if they exist), their niches, and how to use them alongside relevant methods and protocols. This series addresses stem cells during development, homeostasis, and disease/injury of the respective organs, presenting new developments, such as new data on disease and clinical applications. Specific chapters delve into the evolution of hematopoietic stem cell niches, the role of CNS in the regulation of HSCs, and targeting the bone marrow niche in hematological malignancies. In addition, video content illustrates protocols, transplantation techniques, and work with mice.

Enzymes as Sensors, Volume 589, the latest release in the Methods in Enzymology series, covers a variety of topics, including advances in genetically coded fluorescent sensors, enzymes as sensors, and bioapplications of electrochemical sensors and biosensors. Users will find a comprehensive discussion of timely topics that presents a micro-level delivery of specific content related to the study of enzymes in sensors. New to this edition are highly specialized chapters on integrated strategies for gaining a systems level view of dynamic signaling networks, sensitive protein detection and quantification in paper-based microfluidics for point-of-care, and microneedle enzyme sensor arrays for continuous in vivo monitoring. This state-of-the-art series is ideal for those interested in the latest information on enzymology, with this edition focusing on sensors and their role in enzymes.

Ticks of Trinidad and Tobago: An Overview explores tick species prevalent in Trinidad and Tobago (T&T), their distribution, associated pathogens, their effects on the host, and control methods. The book also reviews the basic biology of ticks. Ticks are known to parasitize a wide range of hosts including mammals, reptiles and birds. These parasites are of veterinary and public health significance since they are responsible for the spread of a number of pathogens to humans and animals. Worldwide, ticks are responsible for billions of dollars in losses in the livestock industry annually due to the effects of these pathogens. Based on review of the literature from more than five decades, twenty-three species of both hard and soft tick have been discovered on the twin-island republic with a greater number of species in Trinidad. Tick genera observed and recorded included Argas, Ornithodoros, Amblyomma, Dermacentor, Haemaphysalis, Ixodes, and Rhipicephalus species. The tick species found in Trinidad and Tobago parasitize both wild and domestic species. Hosts include bats, fowl, equids, wild and domestic ruminants, birds, rodents, marsupials, and a variety of reptiles such as toads, tortoises, and snakes. Based on geographical location, most tick species discovered in T&T have also been recorded in other Caribbean islands in the archipelago, North, Central and South America. Both soft and hard tick species found in T&T have also been implicated in a number of blood-borne pathogens including Borrelia, Ehrlichia, Babesia, Hepatozoon, Rickettsia, and Anaplasma.

Natural products in the plant and animal kingdom offer a huge diversity of chemical structures that are the result of biosynthetic processes that have been modulated over the millennia through genetic effects. With the rapid developments in spectroscopic techniques and accompanying advances in high-throughput screening techniques, it has become possible to isolate and then determine the structures and biological activity of natural products rapidly, thus opening up exciting opportunities in the field of new drug development to the pharmaceutical industry. This series covers the synthesis or testing and recording of the medicinal properties of natural products, providing cutting edge accounts of the fascinating developments in the isolation, structure elucidation, synthesis, biosynthesis and pharmacology of a diverse array of bioactive natural products.

Proteomics in Biology Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field, and a focus on proteomics for this updated volume.

Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion.

Enzymology at the Membrane Interface, the latest volume in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.

Developmental Signaling in Plants, the latest volume in The Enzymes series, follows up on the themes discussed in volume 35, notably cell-to-cell and organ-to-organ communication. In addition, it looks at the environmental and hormonal effects on development and the epigenetics on development.

De Novo Enzyme Design, the newest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume includes the design of metal binding maquettes, insertion of non-natural cofactors, Cu metallopeptides, non-covalent interactions in peptide assemblies, peptide binding and bundling, heteronuclear metalloenzymes, florinated peptides, De Novo imaging agents, and protein-protein interaction.

cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging.

Synthetic Biology and Metabolic Engineering in Plants and Microbes, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods, synthetic biology, and metabolic engineering in plants and microbes, and includes sections on such topics as the usage of integrases in microbial engineering, biosynthesis, and engineering of tryptophan derived metabolites, regulation and discovery of fungal natural products, and elucidation and localization of plant pathways.

Amphioxus Immunity: Tracing the Origin of Human Immunity covers a remarkable range of information about Amphioxus and its evolutionary context. This compilation of what is currently known about Amphioxus, with a sharp focus on its immune system, includes 13 topics, such as: Amphioxus as a model for understanding the evolution of vertebrates basic knowledge of immunology immune organs and cells of amphioxus a genomic and transcriptomic view of the Amphioxus immunity pattern recognition system in Amphioxus transcription factors in Amphioxus the complement system of Amphioxus the oxidative burst system in Amphioxus immune effectors in Amphioxus lipid signaling of immune response in Amphioxus apoptosis in amphioxus; primitive adaptive immune system of Amphioxus and future research directions This valuable reference book is loaded with information that will be useful for anyone who wishes to learn more about the origin of vertebrates and adaptive immunity.

TRP Channels as Therapeutic Targets: From Basic Science to Clinical Use is authored by experts across academia and industry, providing readers with a complete picture of the therapeutic potential and challenges associated with using TRP channels as drug targets. This book offers a unique clinical approach by covering compounds that target TRP channels in pre-clinical and clinical phases, also offering a discussion of TRP channels as biomarkers. An entire section is devoted to the novel and innovative uses of these channels across a variety of diseases, offering strategies that can be used to overcome the adverse effects of first generation TRPV1 antagonists. Intended for all researchers and clinicians working toward the development of successful drugs targeting TRP channels, this book is an essential resource chocked full of the latest clinical data and findings.