TRP Channels as Therapeutic Targets: From Basic Science to Clinical Use is authored by experts across academia and industry, providing readers with a complete picture of the therapeutic potential and challenges associated with using TRP channels as drug targets. This book offers a unique clinical approach by covering compounds that target TRP channels in pre-clinical and clinical phases, also offering a discussion of TRP channels as biomarkers. An entire section is devoted to the novel and innovative uses of these channels across a variety of diseases, offering strategies that can be used to overcome the adverse effects of first generation TRPV1 antagonists. Intended for all researchers and clinicians working toward the development of successful drugs targeting TRP channels, this book is an essential resource chocked full of the latest clinical data and findings.

Even the most cursory survey of the chemical literature reveals that modern NMR spectroscopy has indeed fulfilled its potential as a powerful and indispensable tool for probing molecular structure, providing detail that is comparable to, and sometimes surpasses that, of X-ray crystallography. As NMR spectroscopy's 70th anniversary approaches, the diversity of chemical problems to which this technique can be applied continues to grow across many scientific fields. Beyond the laboratory setting, the technology underlying NMR is now a widely used and critical medical diagnostic technique, Magnetic Resonance Imaging (MRI). Unfortunately, the number of applications of NMR spectroscopy across so many STEM-related fields presents significant challenges in how best to introduce this powerful technique in meaningful ways at the undergraduate level. Inspired by the development of the field, and building upon the work of previous symposia and an ACS symposium series book on this topic (3), a symposium was developed, entitled "NMR Spectroscopy in the Undergraduate Curriculum," for the 239th American Chemical Society National Meeting in San Francisco. This book brings together all of the presenters who have been successful in developing and successfully integrating NMR spectroscopy pedagogy across their undergraduate curriculums. Their knowledge and experiences will aid readers who are interested in expanding and invigorating their own curriculum.

Protein folding is a process by which a protein structure assumes its functional shape of conformation, and has been the subject of research since the publication of the first software tool for protein structure prediction. Protein folding in silico approaches this issue by introducing an ab initio model that attempts to simulate as far as possible the folding process as it takes place in vivo, and attempts to construct a mechanistic model on the basis of the predictions made. The opening chapters discuss the early stage intermediate and late stage intermediate models, followed by a discussion of structural information that affects the interpretation of the folding process. The second half of the book covers a variety of topics including ligand binding site recognition, the "fuzzy oil drop" model and its use in simulation of the polypeptide chain, and misfolded proteins. The book ends with an overview of a number of other ab initio methods for protein structure predictions and some concluding remarks.
Discusses a range of ab initio models for protein structure predictionIntroduces a unique model based on experimental observationsDescribes various methods for the quantitative assessment of the presented models from the viewpoint of information theory

In recent years, a number of academic and commercial software packages and databases have been developed for the analysis and screening of biological data; however, the usability of these data is compromised by so-called novel genes to which no biological function is assigned. Annotating new genes outlines an approach to the analysis of evolutionary-conserved, heart-enriched genes with unknown functions, offering a step-by-step description of the procedure from screening to validation. The book begins by offering an introduction to the databases and software available, before moving on to cover programming guidelines, including a specific case study on the use of C-It for in silico screening. The second half of the book offers a step-by-step guide to experimental validation concepts and procedures, as well as an overview of additional potential applications of this approach in the field of stem cells and tissue regeneration, before a concluding chapter summarises the concepts and theories presented.
Focuses not only on screening but also on biological validationsProvides details of databases and software (web interface) products for biologists with minimal computation skillsOffers a step-by-step outline of the procedure involved

Therapeutic protein drug products provides a comprehensive overview of therapeutic protein drug products, with an emphasis on formulation beginning in the laboratory, followed by manufacturing and administration in the clinic. A list of many commercial therapeutic drug products are described and include the product name, dosages, active concentration, buffer, excipients, Ph, container type and route of administration. The laboratory formulation sections focus on the most common buffers, excipients, and Ph ranges that are commonly tested in addition to systematic approaches. A brief section on biophysical and analytical analysis is also provided. Properties of therapeutic protein formulations are described and include opalescence, phase separation, color, and subvisible particles. An emphasis is placed on material and process testing to ensure success during manufacturing. The drug product manufacturing process, which includes the process of compounding to filling, is also covered. Methods of delivery in the clinic are addressed, as well as delivery strategies. Finally, a perspective on the regulatory requirements for therapeutic protein formulations is discussed.
Provides a list and description of commercially available therapeutic drug products and their formulationsA comprehensive and practical overview of protein formulation in the laboratory, manufacturing, and the clinicDiscusses recent topics including high protein concentration, phase separation, opalescence, and subvisible particles

This MIE volume provides laboratory techniques that aim to predict the structure of a protein which can have tremendous implications ranging from drug design, to cellular pathways and their dynamics, to viral entry into cells.
Expert researchers introduce the most advanced technologies and techniques in protein structure and folding
Includes techniques on tiling assays.

The 2e of this classic "Guide to Protein Purification" provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today.
Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less common relevant techniquesProvides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process"

Written by an immunologist, this book traces the concept of immunity from ancient times up to the present day, examining how changing concepts and technologies have affected the course of the science. It shows how the personalities of scientists and even political and social factors influenced both theory and practice in the field. With fascinating stories of scientific disputes and shifting scientific trends, each chapter examines an important facet of this discipline that has been so central to the development of modern biomedicine. With its biographical dictionary of important scientists and its lists of significant discoveries and books, this volume will provide the most complete historical reference in the field.
Written in an elegant style by long-time practicing immunologist
Discusses the changing theories and technologies that guided the field
Tells of the exciting disputes among prominent scientists
Lists all the important discoveries and books in the field
Explains in detail the many Nobel prize-winning contributions of immunologists"

Levels of mycotoxin contamination in agricultural commodities are regulated in more than 100 countries, and exposure to these naturally occurring toxins presents serious risks to the health of humans and animals with negative impacts to commodity values. This symposium series book presents significant scientific developments in the multifaceted approach to reducing exposure to these naturally occurring toxins. A broad-spectrum of subject matter of the multidisciplinary field of mycotoxin research is conveniently compiled in this single volume, and general themes include prevention, control, exposure, molecular biology, biosynthesis, analytical methodology, and emerging techniques. The book opens with an overview of prevention of mycotoxin production by means of biological control and human exposure to contaminated foods, including tofu, apples, figs, and a broad range of fruits.
The second section focuses on molecular biological approaches to control mycotoxin exposure. Topics covered include genomic approaches, detoxification, structure-activity studies of biosynthetic precursors, and mycotoxin biosynthesis. The success of these strategies is supported by the reduction of mycotoxin occurrence and incidence in foods. Accurate estimates of mycotoxin concentration in foods are important for food safety and wholesomeness.
The final section covers analytical approaches, including sampling plans for foods and dietary supplements, extraction efficiency of mycotoxins from naturally contaminated grains, traditional detection methods such as chromatography/mass spectrometry, and emerging techniques such as the use of molecularly imprinted polymers for purification and isolation and cyclodextrins for fluorescent enhancement of several mycotoxins.

Since its inception in 1945, this serial has provided critical and integrating articles written by research specialists that integrate industrial, analytical, and technological aspects of biochemistry, organic chemistry, and instrumentation methodology in the study of carbohydrates. The articles provide a definitive interpretation of the current status and future trends in carbohydrate chemistry and biochemistry.

"Advances in Planar Lipid Bilayers and Liposomes, " Volume 8, continues to include invited chapters on a broad range of topics, covering both main arrangements of the reconstituted system, namely planar lipid bilayers and spherical liposomes. The invited authors present the latest results in this exciting multidisciplinary field of their own research group.
Many of the contributors working in both fields over many decades were in close collaboration with the late Prof. H. Ti Tien, the founding editor of this book series. There are also chapters written by some of the younger generation of scientists included in this series. This volume keeps in mind the broader goal with both systems, planar lipid bilayers and spherical liposomes, which is the further development of this interdisciplinary field worldwide.
* Incorporates contributions from newcomers and established and experienced researchers
* Explores the planar lipid bilayer systems and spherical liposomes from both theoretical and experimental perspectives
* Serves as an indispensable source of information for new scientists

Phospholipid technology and applications is an essential reference for technologists developing food and cosmetics products, scientists researching phospholipids in biological and food systems, technologists in fats and oils refining, and scientists developing drugs and drug delivery systems and carriers.
The major source of phospholipids is the lecithin recovered during degumming of vegetable oils, particularly soybean oil. This crude material finds uses in its own right but can be purified through a series of processes which eventually lead to individual phospholipid classes such as phosphatidylcholines.
It is widely accepted that oil and water do not mix but there are several areas in science and technology where these two distinct phases must coexist in stable emulsions. This is achieved by admixture of amphiphilic molecules of which the phospholipids are important natural examples. Today, phospholipids find many uses in the food industry and in other industries which exploit the amphiphilic nature of these compounds. Further, there are now important procedures by which their amphiphilicity can be optimized for different uses.
The early chapters in this book are devoted to the more common glycerol-based phospholipids and cover their structure, source, composition, modification by chemical and enzymatic methods, their physical, chemical, and nutritional properties, and their major uses. The final chapter is devoted to another kind of phospholipid, the sphingolipids, in which there is a growing interest.

Advances in Planar Lipid Bilayers and Liposomes, Volume 6, continues to include invited chapters on a broad range of topics, covering both main arrangements of the reconstituted system, namely planar lipid bilayers and spherical liposomes. The invited authors present the latest results in this exciting multidisciplinary field of their own research group.
Many of the contributors working in both fields over many decades were in close collaboration with the late Prof. H. Ti Tien, the founding editor of this book series. There are also chapters written by some of the younger generation of scientists included in this series. This volume keeps in mind the broader goal with both systems, planar lipid bilayers and spherical liposomes, which is the
further development of this interdisciplinary field worldwide.
* Contributions from newcomers and established and experienced researchers
* Exploring theoretically and experimentally the planar lipid bilayer systems and spherical liposomes
* This volume is dedicated to mark the Bilayer Lipid Membranes 45th anniversary

Chirality as an environmental phenomenon was dealt with in a thorough and interesting manner in a series of three symposia entitled "Modern Chiral Pesticides: Enantioselectivity and Its Consequences," sponsored by the Agrochemical Division of the American Chemical Society and held in Washington, DC (2005), Boston, MA (2007) and San Francisco, CA (2010). All three symposia included speakers from industry, government and academia, representing several European countries, China, and the United States. Corresponding to this broad group of countries, institutions and speakers, the range of topics touched on almost all facets of chirality as it is manifested in environmental and human exposure and toxicity. The 40 oral and 20 poster presentations indeed approached comprehensive coverage: analysis of enantiomers and other stereoisomers; preparative separation of enantiomers; stereoselective occurrences of chiral pesticides in environment soil and water and in wildlife and human tissues and fluids; stereoselective degradation and metabolism of chiral pesticides; and stereoselective toxicity.
This book is a result of manuscript contributions by some of the oral and poster presenters to the third symposium in 2010. In addition to symposium participants, invitations were extended to the environmental chiral chemistry community in general, including most of the speakers in the 2005 and 2007 symposia, in an attempt to attain good coverage of this rather broad topic. Thus, this ACS Symposium book will generate many new ideas from interested readers and inform them of useful techniques for experimental exploration of the somewhat exotic, but important, area of chiral chemistry of pesticides.

Amyloids, Prions and Beta Proteins is the last volume of the three-part thematic series on Fibrous Proteins in the Advances in Protein Chemistry serial. Fibrous proteins act as molecular scaffolds in cells providing the supporting structures of our skeletons, bones, tendons, cartilage, and skin. They define the mechanical properties of our internal hollow organs such as the intestines, heart, and blood vessels. This volume covers such topics as Beta-Structures in Fibrous Proteins; B-Silks: Enhancing and Controlling Aggregation; Beta-Rolls, Beta-Helices and Other Beta-Solenoid Proteins; Natural Triple B-Stranded Fibrous Folds; Structure, Function and Amyloidogenesis of Fungal Prions: Filament Polymorphism and Prion Variants; X-Ray Fiber and powder Diffraction of PRP Prion Peptides; From the Polymorphism of Amyloid Fibrils to Their Assembly Mechanism and Cytotoxicity; Structural Models of Amyloid-like Fibrils.

This volume is an in-depth treatment of G-Protein Signaling, Part A, and will cover general methods of analysis of RGS protein analysis, including Expression and post-translational modification, Assays of GAP activity and allosteric control, Electrophysiological methods and RGS-insensitive Ga subunits, Mouse models of RGS protein action, Methods of RGS protein inhibition, and G-protein regulators of model organisms.
Table of Contents
-Expression and post-translational modification
-Assays of GAP activity and allosteric control
-Electrophysiological methods and RGS-insenstitive Ga subunits
-Mouse Models of RGS protein action
-Methods of RGS protein inhibition
-G-protein regulators of model organisms

In this second edition, Edwin Frankel has updated and extended his now well-known book Lipid oxidation which has come to be regarded as the standard work on the subject since the publication of the first edition seven years previously. His main objective is to develop the background necessary for a better understanding of what factors should be considered, and what methods and lipid systems should be employed, to achieve suitable evaluation and control of lipid oxidation in complex foods and biological systems.
The oxidation of unsaturated fatty acids is one of the most fundamental reactions in lipid chemistry. When unsaturated lipids are exposed to air, the complex, volatile oxidation compounds that are formed cause rancidity. This decreases the quality of foods that contain natural lipid components as well as foods in which oils are used as ingredients. Furthermore, products of lipid oxidation have been implicated in many vital biological reactions, and evidence has accumulated to show that free radicals and reactive oxygen species participate in tissue injuries and in degenerative disease.
Although there have been many significant advances in this challenging field, many important problems remain unsolved. This second edition of Lipid oxidation follows the example of the first edition in offering a summary of the many unsolved problems that need further research. The need to understand lipid oxidation is greater than ever with the increased interest in long-chain polyunsaturated fatty acids, the reformulation of oils to avoid hydrogenation and trans fatty acids, and the enormous attention given to natural phenolic antioxidants, including flavonoids and other phytochemicals.

A combination of population growth, public health failures, environmental degradation and rapid global transportation has resulted in a world that is at increasing risk to vectorborne and other infectious diseases. A large percentage of emerging diseases are vectorborne and over one-third of the agents on the list of greatest concern from bioterrorism are vectorborne. Many of these diseases are viral that have no effective drug or vaccine treatments. Drug and insecticide resistance is now common and has greatly compromised our ability to provide effective and affordable control. Parasitic diseases, including malaria, leishmmaniasis and African trypanosomiasis are likewise increasing in many parts of the world. Control programs for onchocerciasis and to some extent filariasis are reducing the impact of these diseases, largely due to the availability of filaricides such as ivermectin. Chagas disease has also declined significantly through home improvements and indoor insecticide application against the domicilary kissing bug vectors.
Despite these gains, this tend has not been sustainable. Instead, infectious disease is now responsible for greater than 25% of all deaths and nearly 50% of premature deaths among those under 45 years of age, and 63% for children less than 4 years of age. A significant proportion of these deaths is attributed to vectorborne diseases, particularly from malaria ( 11%). Indeed, more that 1 million people are killed annually by malaria, about 3000 per day. It is estimated that 700,000 children under the age of 5 die of malaria and at least 300 million are ill due to malaria each year. In response, the American Institute of Medicine (2003) has called for a renewed effort to rebuild public health infrastructures needed to conduct disease surveillance and vector control programs and to increase research to provide improved pesticides and their use, new repellents, new biopestcides and biocontrol agents to augment pesticidal control, as well as novel strategies to interrupt pathogen transmission.
With these goals in mind, we convened the first vector control symposium as part of the scientific program of the 3rd Pan-Pacific Conference of Pesticide Science in 2003. Five years after (2008), we re-convened this expanded topic at the 4th Pan-Pacific Conference on Pesticide Science and the scientific presentations made over two days comprise this current volume, Advances in Human Vector Control. The book covers two major areas: 1) Current Status and Control Practices, covering malaria, dengue, Chagas, human lice, cockroach and house dust mite issues; and 2) Novel Approaches and Resistance Management of these diseases. Chapters are provided by internationally-recognized experts who are actively involved in vector control and management, providing an up to date summary of this critically important area of public health. The effective use of novel control strategies is stressed and the status of recently acquired genomic approaches is critically reviewed.

Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies.
*Methods in Liposome Preparation
*Physiochemical Characterization of Liposomes

This ACS Symposium Series is the product of a symposium held at the 241st National Meeting of the American Chemical Society in Anaheim, CA on March 27-31, 2011. It includes chapters on new biobased building blocks such as the furandicarboxylic acid, polyesters and polyamides from adipic, succinic and sebacic acids with aliphatic diols such as 1,3-propylene glycol, 1,4-butanediol, 1,12-dodecylenediol and isosorbide. The conversion of hydroxymethylfurfural, the dehydration product of hexose sugars, to succinic acid and 1,4-butanediol to produce poly(butylene succinate) is described in one chapter. Also the synthesis of new polymers from plant-derived olefinic monomers such as tulipalin A and studies of composites from cotton by-products are featured in other chapters. There is a strong emphasis on biocatalytic synthesis and polymerization within the book. Chapter topics include the synthesis of ?-hydroxyfatty acids and polymers therefrom, an interesting discussion on the structural differences of the products of the biocatalytic and chemical catalytic synthesis of polyesters from oleic diacid and glycerol and the ability to produce polylactic acid (PLA) and PLA-PHA copolyesters within a "microbial cell factory". Other areas of interest explored in other chapters include recent developments of biobased polymer fibers and oleate-based pressure sensitive adhesives and composites. One chapter describes a large increase in cold-drawn fiber tensile strength by the blending of a small amount of ultrahigh molecular weight (MW) poly(3-hydroxybutyrate) with a much lower MW 3-hydroxybutyrate polymer. The addition of a rubber and inorganic fillers to normally brittle PLA was found to dramatically improve its ductility. Finally, there are several chapters on seed oil-based polyurethanes, one on fibers from soy proteins and composites from starch.

Microbiological risk assessment (MRA) is one of the most important recent developments in food safety management. Adopted by Codex Alimentarius and many other international bodies, it provides a structured way of identifying and assessing microbiological risks in food. Edited by two leading authorities, and with contributions by international experts in the field, Microbiological risk assessment provides a detailed coverage of the key steps in MRA and how it can be used to improve food safety.
The book begins by placing MRA within the broader context of the evolution of international food safety standards.
Part one introduces the key steps in MRA methodology. A series of chapters discusses each step, starting with hazard identification and characterisation before going on to consider exposure assessment and risk characterisation. Given its importance, risk communication is also covered. Part two then considers how MRA can be implemented in practice. There are chapters on implementing the results of a microbiological risk assessment and on the qualitative and quantitative tools available in carrying out a MRA. It also discusses the relationship of MRA to the use of microbiological criteria and another key tool in food safety management, Hazard Analysis and Critical Control Point (HACCP) systems.
With its authoritative coverage of both principles and key issues in implementation, Microbiological risk assessment in food processing is a standard work on one of the most important aspects of food safety management.
Provides a detailed coverage of the key steps in microbiological risk assessment (MRA) and how it can be used to improve food safetyPlaces MRA within the broader context of the evolution of international food safety standardsIntroduces the key steps in MRA methodology, considers exposure assessment and risk characterisation, and covers risk communication

This volume and its companion Volume 351 will supplement Volume 194 of MIE. The guides are specifically designed to meet the needs of graduate students and postdocs as well as researchers. Whether an established researcher or newcomer to the field, these volumes will contain all the up-to-date methods needed to study "Genes in Yeast." Procedures are included to enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information will be given for proven procedures that can be used as a guide for developing protocols in a number of disciplines.

This is the first book solely devoted to single-molecule biochemistry and molecular biology. Authors were selected on the basis of their contribution to this new and exciting field, and were asked to focus more on the biological problems that can be approached using single-molecule techniques rather than on the techniques per se. It is thought that such techniques will eventually dominate the physical characterization of biologically important macromolecules.

People working in development of drugs, pesticides, washing detergents, etc., are obliged by law to conduct analyses of the "metabolic pathways" or "maps" for the chemical compounds that they are using or proposing.
Everyone in these industries or carrying out research toward such products is therefore interested in having a reference on these compounds.
Key Features
* Covers literature relating to degradation and metabolic profiles of molecules
* Draws together the literature on degradation and metabolism patterns with that on diversified conditions of the environmental systems
* Indicates 3D chemical structures, predicted physico-chemical parameters, logP and the SMILES chemical notations of the parent compounds to assist scientists design the lead compounds for new discovery using computer-aided technology

Natural products play an integral and ongoing role in promoting numerous aspects of scientific advancement, and many aspects of basic research programs are intimately related to natural products. The significance, therefore, of the Studies in Natural Product Chemistry series, edited by Professor Atta-ur-Rahman, cannot be overestimated. This volume, in accordance with previous volumes, presents us with cutting-edge contributions of great importance.