Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as DNA and protein engineering to create protein bioswitches with new functions, Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases, An experimental protocol to study lipid transfer proteins, Synthesis of small heat shock proteins, Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins, An experimental protocol for glycoconjugate analysis, Methods for proximity-based biotinylation combined with Mass Spectrometry, and more. Additional chapters cover Synthetic antibody fragments as conformational sensors of protein activation and trafficking, Expression, purification, functional analysis and crystallization of Rag GTPase, Purification of bacterial transcription elongation complexes by photoreversible immobilization, Inhibition of c-Myc-MAX heterodimerization, Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases, and much more.

Advances in Biomolecular EPR, Volume 666 in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics including Magnetic Resonance Characterization of Physiologically Important Metal Ion Binding Sites in the Prion and Related Proteins, The catalytic role of metal-radical/protein-based radicals in heme enzymes, Rigid Cu2+-based spin labels for the study of higher-order DNA G-quadruplex structures, Orthogonal spin labeling and membrane proteins: increasing the information content and going towards in cell applications, Spectroscopic investigation of mono- and di-Mn-containing centers in biochemistry with an emphasis on application of paramagnetic resonance techniques, and more. Additional chapters cover In Vivo pO2 Imaging of Tumors: Oxymetry with Very Low-Frequency Electron Paramagnetic Resonance, an Update, EPR contributions to understanding molybdenum-containing enzymes, EPR spectroscopy of Type I reaction centers, Characterization of a substrate-derived radical in the NosN reaction during the biosynthesis of nosiheptide, and much more.

RNA Modification Enzymes, Volume 659 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of related topics, including Locating chemical modifications in RNA sequences through ribonucleases and LC-MS based analysis, Development of RNA modification mapping pipelines for high-throughput sequencing approaches, AlkAniline-Seq for high-resolution mapping RNA m7G and m3C modifications, Facile detection of RNA phospho-methylation in cells, Detection and analysis of glycosylated queuosine modifications, A comprehensive pipeline for analysis of RNA 3'-end modification, Analysis of the epitranscriptome with ion-pairing reagent free oligonucleotide mass spectrometry, and more.

The Microbiome, Volume 176, assembles known facts and provides guidance for their implementation on topics relating to associations between the gut microbiome and personality traits, depression, anxiety, autism, schizophrenia, cognition, dementia and neurodegeneration. Additionally, this volume considers the influence of the maternal microbiome on brain development, with chapters covering Intervention, prevention, and the brain: prebiotics, probiotics, and fecal transplants, The microbiota-gut-brain axis: focus on the fundamental communication pathways, and Microbiome composition and locations.

Advances in CRISPR/Cas and related technologies, Volume 179, the latest release in this ongoing series, deals with a wide variety of research topics related to recent advancement in the genome editing techniques. Associated chapters in this new release include Challenges for Therapeutic application of CRISPR Cas techniques, Mitochondrial DNA modification by CRISPR Cas System: Challenges and future direction, Trends in CRISPR Cas technology application in cancer, Modified CRISPR-Cas for next generation application, Application of CRISPR Cas in Synthetic Biology: Challenges and Scopes, History of CRISPR Cas system from bacterial Adaptive Immune System to research application, and more.

This book deals with an array of topics in the broad area of abiotic stress responses in plants focusing "problems and their management" by selecting some of the widely investigated themes. Such as, Cell signalling in Plants during abiotic and biotic stress, Salinity stress induced metabolic changes and its management, High temperature stress: responses, mechanism and management, Low temperature stress induced changes in plants and their management, Biotechnological approaches to improve abiotic stress tolerance, Nutritional poverty in wheat under abiotic stress scenario, Strategies for improving soil health under current climate change scenario, Abiotic stress management in Pulse crops, Mitigation strategies of abiotic stress in fruit crops, Impacts of abiotic stress and possible management option in vegetable crops, and Abiotic stress: impact and management in ornamental crops. This book is useful for under-graduate and post-graduate students in Plant Physiology, Biochemistry, agronomy, horticulture, Botany, Environmental sciences and other cognate disciplines of agriculture and allied sciences and other research workers. We fervently believe that this book will provide good information and understanding of abiotic stress problems and their management in plants

This book deals with an array of topics in the broad area of biotic stress responses in plants focusing "problems and their management" by selecting some of the widely investigated themes. Such as, Major insect-pest of cereal crops in India and their management, Biotic stresses of major pulse crops and their management strategies, Insect pest of oilseed crops and their management, Biotic stresses of vegetable crops & their management, Insect pests infesting major vegetable crops and their management strategies, Fruit Crops Insect pests and their Biointensive Integrated Pest Management techniques, Mass Trapping of fruit flies using Methyl Eugenol based Traps, Organic means of combating biotic stresses in plants, Nematode problem in pulses and their management, and approaches in pest management of stored grain pests. This book is useful for under-graduate and post-graduate students in Entomology, Plant Pathology, Agronomy, Horticulture, other cognate disciplines of agriculture and allied sciences and other research workers. We fervently believe that this book will provide good information and understanding of biotic stress problems and their management in plants.

Advances in Microbial Physiology, Volume 150 in this important serial, highlights new advances in the field with this new volume including content by an international board of authors. Chapters in this new release include Organization of respiratory chains in the bacterial cell, Anaerobic methane oxidizing archaea, Dawn of the DedA: the structure and function of the DedA family of integral membrane proteins associated with bacterial viability and antimicrobial resistance, Nickel, an essential virulence determinant of Helicobacter pylori: trafficking pathways and their targeting by bismuth, Dissimilatory sulfur compounds oxidation in thermophilic and chemolithoautotrophic bacteria belonging to the Aquificales order, and much more.

The volume deals with other aspect (s) of metabolic activities of plants under abiotic stress. This book discusses techniques for improving yield potential and adaptiveness to unfavorable environmental conditions. This book also addresses how knowledge of the changes in physiological mechanisms can contribute in understanding the yield structure under abiotic stress. 1. Abiotic Stress and Metabolic Responses in Plants 2. Abiotic Stress and Secondary Metabolites in Plants 3. Microbes: Support and Protect Plants against Abiotic Stresses 4. Abiotic Stress and Physiological Traits in Plants 5. Abiotic Stress and Heat Shock Proteins (HSPs) in Plants

Ion Channels Part B, Volume 652 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including NMDAR, Pannexin, and CALHM, Making NaV1.4 and NaV1.7, TRPVs, Purification native nAChRs, GABAR Radu Aricescu, TRPV5/2, NaV1.5, KATP, TRPA1, TREK-1, SARS-CoV-2 3a ion channel, Ion channel conformational dynamics by encoded unnatural amino acid, Fluorescence lifetime measurement of absolute membrane potential, Fluorescent Toxins as Activity Sensors, FRET Analyses of Ion Channel Protein-Protein Interactions, Control of Ion Channel Gating with Photo-Switchable Tweezers, and Counting Subunits in Kv Channel Complexes.

DNA Repair, Volume 45 in The Enzymes series highlights new advances in the field, with this new volume presenting interesting chapters on DNA polymerase beta and other gap-filling enzymes in mammalian base excision repair, Translesion polymerases, mechanism and function, The Rev1-Pol? Mutasome: Structure and Interactions in Translesion Synthesis, Challenges for base excision repair enzymes: acquiring access to damaged DNA in chromatin Nucleotide excision repair, DNA damage recognition mechanisms in mammalian nucleotide excision repair, Advances in understanding DNA mismatch repair, and more.

Ion Channels Part A, Volume 651 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of new developments on the topic. Each chapter is written by an international board of authors.

Abiotic stress factors frequently constrain the growth and productivity of major crop species. The single greatest abiotic stress factor that limits crop growth worldwide is water availability. While genetic increases in yield potential are best expressed in optimum environments, they are also associated with enhanced yields under drought and nitrogen deficiency. These gains are especially relevant given that further large increases in the area under irrigation are not expected, and land deterioration associated with intensive agriculture threatens those areas already irrigated. This book is intended to cover all known factors of abiotic stresses and their respective effects on some of the important aspects of physiological processes in plants.1. Abiotic Stress and Crop Yield 2. Physiology of Grain Legume Yield Under Abiotic Stress 3. Photosynthesis and Physiological Parameters Under Intercropping Condition 4. Role of Growth Regulators Under Abiotic Stress 5. Plant Water Relationship Under Abiotic Stress 6. Dry Matter Partitioning Under AbioticS tress 7. Effect of Pesticides on Physiological Processes in Plants

Enzymatic Polymerizations, Volume 627 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.