This volume is a revision of Biological Control by R. van den Bosch and P. S. Messenger, originally published by Intext Publishers. In the revision, I have attempted to keep the original theme, and to update it with current research findings and new chapters or sections on insect pathology, microbial control of weeds and plant pathogens, population dynamics, integrated pest management, and economics. The book was written as an undergraduate text, and not as a complete review of the subject area. Various more comprehen sive volumes have been written to serve as handbooks for the experts. This book is designed to provide a concise overview of the complex and valuable field of biological control and to show the relationships to the developing concepts of integrated pest management. Population regulation of pests by natural enemies is the major theme of the book, but other biological methods of pest control are also discussed. The chapter on population dynamics assumes a precalculus-level knowledge of mathematics. Author names of species are listed only once in the text, but all are listed in the Appendix. Any errors or omissions in this volume are my sole responsibility. A. P. Gutierrez Professor of Entomology Division of Biological Control University of California, Berkeley vii Acknowledgments Very special thanks must be given to my colleagues, Professors C. B. Huffaker and L. E. Caltagirone, for the very thorough review they provided and for the many positive suggestions they gave. Dr."

The Fourteenth Midwest Conference on Endocrinology and Metab olism, held at the University of Missouri - Columbia on September 28th and 29th, 1978, brought together several prominent researchers who are authorities on various aspects of the renin-angiotensin system. Each speaker presented an in-depth coverage of a topic related to his own area of expertise, including recent findings from his own research laboratory. Following each presentation thet:e was a general discussion of the material by the speaker and the audience. These presentations and the ensuing discussions are summarized in these published Proceedings. Traditionally the Midwest Conferences on Endocrinology and Metabolism have emphasized breadth as well as depth of coverage of the selected topic; the present Conference is no exception. Perusal of the titles of the presentations will reveal that the Conference dea1th with many different facets of the renin-angiotensin system, including the biochemistry, anatomy, physiology, and comparative endocrinology of this hormonal system, plus special areas of con sideration such as angiotensin receptors, angiotensin-converting enzyme, the control of renin release, angiotensin and aldosterone secretion, and the role of the renin-angiotensin system in the central nervous system. The selection of the renin-angiotensin system as the topic for the present conference was very timely because of the many noteworthy advances in this area in recent years, many by the participants in the Conference. The Editors are very appreciative of the excellent manuscripts which the speakers provided for these Proceedings.

Emphasis in agricultural research for many years has concen trated on crop production. This emphasis has become more important in recent years with the realization that the population worldwide is outstripping the food supply. There is, however, another side to increasing the availability of the food supply. This simply involves preservation of the harvested crop.for human consumption. The losses incurred in harvesting, handling, transportation, storage and marketing crops have become a greater problem as the distance from the farm to the ultimate consumer increases. In the Western world where modern transportation, storage facilities, and marketing technology are widely used, post-harvest technology requires a large input of energy which increases costs considerably. There fore, losses are more significant and the ability to provide fresh fruits and vegetables, out of season, at reasonable costs will depend on reduced post-harvest losses throughout the marketing chain from the farm gate to the ultimate consumer. The reduction in post-harvest losses depends on proper use of current technology and further developments derived from a broad spectrum of scientific disciplines. Biochemistry, plant physiology, plant pathology, horticulture, agronomy, physics, engineering and agricultural economics, all provide knowledge which has been useful and will be useful in the future for improving post-harvest technol ogy and crop preservation. This volume records the Proceedings of the NATO Advanced Study Institute on Post-Harvest Physiology and Crop Preservation, held at Sounion, Greece, April 28 - May 8, 1981."

Gilbert S. Omenn Dean, Public Health and Community Medicine University of Washington Seattle, Washington 98195 On behalf of the University of Washington, the City of Seattle, the sponsors and donors, and my co-organizers, I am delighted to welcome all of you to this Conference on Genetic Control of Environ mental Pollutants. My only regret is that Dr. Alexander Hollaender, who has inspired so many of us as young scientists and stimulated so many trail-blazing conferences in environmental sciences and in gen etic engineering, is ill and was unable to make the trip to Seattle. He sends his warm good wishes for an outstanding meeting and a fine volume. The purpose of this Conference is to identify and assess strat egies for more effectively and safely managing wastes and toxic sub stances in the environment, in part through use of genetically engi neered microorganisms. There is a sense of desperation in our soci ety that modern technologies have introduced a bewildering array of potential hazards to human health and to our environment. There is an accompanying sense of frustration that our prodigious basic re search capabilities and our technological ingenuity have not yielded practical ways to control many pollutants and waste streams, or- better still--to convert them to useful products.

The first international congress for ecology took place in 1974 in The Hague, its central theme being "Unifying Concepts in Ecology". In the forefront of discussion at that time were questions of constancy, stability and resilience. Such questions have gone slightly out of fashion and the exceptionally precise and well thought-out concepts of that era are seldom applied nowadays. The present book introduces another unifying concept, the concept of the ecological cycle, or, more precisely, the mosaic-cycle concept of ecology. The following chapters have their origin in lectures which were held and discussed at a symposium of the Werner Reimers Stiftung in Bad Homburg. The purpose of the symposium was the preparation of this book. Our warmest thanks go to the Reimers Stiftung for their assistance and hospitality. We should also like to express our gratitude to all participants, to those who contributed to the discussion, and above all to those colleagues whose lectures provided, from a variety of aspects, a critical approach to the mosaic-cycle concept. Marburg, Winter 1990/91 HERMANN REMMERT Contents H. REMMERT The Mosaic-Cycle Concept of Ecosystems - An Overview ......... .

The series of books on the biotechnology of Medicinal and Aromatic Plants provides a survey of the literature, focusing on recent information and the state of the art in tissue culture and the in vitro production of secondary metabolites. This book, Medicinal and Aromatic Plants VII, like the previous six volumes published in 1988, 1989, 1991, 1993 and 1994, is unique in its approach. It comprises 28 chapters dealing with the distribu- tion, importance, conventional propagation, micro propagation, tissue culture studies, and the in vitro production of important medicinal and pharmaceutical compounds in various species of Aesculus, Althaea, Baptisia, Berberis, Beta, Bowiea, Camp to theca, Chrysanthellum, Citrus, Claviceps, Coleonema, Dianthus, Dunaliella, Epimedium, Euphorbia, Forsythia, Gomphrena, Larix, Lobelia, Medicago, Papaver, Phytolacca, Pueraria, Santalum, Santolina, Sapium, Tabebuia, and Tripterygium. This book is tailored to the needs of advanced students, teachers, and research scientists in the field of pharmacy, plant tissue culture, phytochemistry, biochemical engineering, and plant biotechnology in general. New Delhi, July 1994 Professor Y. P. S. BAJAJ Series Editor Contents I Aesculus hippocastanum L. (Horse Chestnut): In Vitro Culture and Production of Aescin P. GASTALDO, A. M. CAVIGLIA, and P. PROFUMO (With 7 Figures) 1 General Account ...1 ...2 In Vitro Culture Studies ...4 3 Summary and Conclusions ...10 4 Protocol...11 References ...11 II Althaea officinalis L. (Marshmallow): In Vitro Culture and the Production of Biologically Active Compounds I. IONKovA and A. W. ALFERMANN (With 10 Figures) 1 General Account...13 ...2 Biotechnological Approaches...21 .

The idea of Volums 4 and 5 of this series is a combination of a very condensed but broad review, handbook and textbook on most of the theoretical and practical aspects of pyrethroids of interest to chemists, biologists, pharmacologists, toxicologists and other people involved in insecticidal research, development, ecotoxicolo gy, application, patenting and commercialization of pyrethroids, as seen from a point of view of an industrial chemist once actively in volved in pyrethroid research. In order to provide a mode1 data base for testing quantitative structure activity relationships, the unique wealth of the numerous and diverse biological data dispersed in the literature is listed in about 110 tables, put in relation to each other and other standard compounds, together with many structural formulas of the com pounds involved. A number of data are published for the first time. Some of the more important reeent QSAR-studies are briefly acknowledged. Conformational aspects of bioactive pyrethroids are discussed (from synthesis, QSAR, X-ray); some of the X-ray derived conformations are published for the first time. The molecular basis of pyrethroid biological action is taken into consideration as intensively as possible. Stereochemical aspects are considered wherever they are involved in synthesis, biological activity, mode of action, metabolism (Vol. 4), and particularly in the complex problems in the production and relation of iso merically enriched trade products (Vol. 5). Since pyrethroid research is now, in manyaspeets, a finished chapter of applied research, a historieal treatment of the course of inventions is given (inventions, patent priorities).

This volume characterizes the current state of natural science and socioeconomic modeling of the impacts of climate change and current climate variability on forests, grasslands, and water. It identifies what can be done currently with impact assessments and suggests how to undertake such assessments. Impediments to linking biophysical and socioeconomic models into integrated assessments for policy purposes are identified, and recommendations for future research activities to improve the state of the art and remove these impediments to model integration are provided. This book is for natural and social scientists with an interest in the impacts of climate change on terrestrial and aquatic ecosystems and their socioeconomic impacts, and policy makers interested in understanding the status of current assessment capabilities and in identifying priority areas for future research.

Silvology is the general science of forest ecosystems, without the usual division between Man and Nature. This systematic treatment of forests intends to integrate and harmonize existing approaches with the help of systems modeling in a hierarchy of close system levels, according to criteria of biological architecture, biomass production and species composition. Scientists and practitioners will appreciate this synoptic treatment of forests and their ecology, allowing the balance of holistic and reductionist viewpoints, and the placement of phenomena and techniques. Topics covered include: - introduction of the methods, - sections on forest organisms, - a special chapter on trees, - eco-units, i.e. forest ecosystems developing after some zero-event like fire, storm or waterlogging, - silvatic mosaics built by the eco-units of different size, architecture and species composition, - a summary of silvological rules determining system's behaviour at every level, e.g. fragmentation and fusion, transfer of functions, irreversibility and process oscillation.

H. BINDING and J. REINERT In collaboration with the first authors of this volume This volume is devoted to the development of cell clones and plants from ma nipulated cells: isolated protoplasts, cell fusion bodies, and transformed cells. Isolated protoplasts represent cells which are liberated from their walls and separated from the differentiation pattern of the organism. Investigations on re generation from protoplasts provide a better understanding of the process and control of developmental pathways. Whereas protoplast isolation results in alteration of the state of differentiation of a cell, protoplast fusion is a means for the creation of cells with novel genetic constitution. Fascinating features are (1) to hybridize cells which-unlike gametes -did not derive from meiosis products, (2) to bring together foreign plastids and mitochondria and to investigate their parasexual reactions, and (3) to match ge netic traits which had been separated for long periods of evolution. Highly sophisticated techniques have already been elaborated for the transfer of genes by the use of isolated DNA and gene transfer systems. Highly promising results have already been obtained by the use of Ti plasmids of Agrobacterium, but direct DNA transformation is also proving to be useful. Most of the results in these areas are preliminary and/or limited to a few sys tem.lt is the aim of this volume to present the main features, but at the same time to draw attention to problems and perspectives of protoplast regeneration and so matic cell genetics in order to stimulate further investigations."

27 chapter cover the distribution, economic importance, conventional propagation, micropropagation, tissue culture, and in vitro production of important medicinal and pharmaceutical compounds in various species of Ajuga, Allium, Ambrosia, Artemisia, Aspilia, Atractylodes, Callitris, Choisya, Cinnamomum, Coluria, Cucumis, Drosera, Daucus, Eustoma, Fagopyrum, Hibiscus, Levisticum, Onobrychis, Orthosiphon, Quercus, Sanguinaria, Solanum, Sophora, Stauntonia, Tanecetum, Vetiveria, and Vitis. Like the previous volumes 4, 7, 15, and 21 in the Medicinal and Aromatic Plants series, the volume is tailored to the need of advanced students, teachers, and research scientists in the area of plant biotechnology andbioengineering, pharmacy, botany and biochemistry.

"Forests in Development: A Vital Balance," shows some of the main advances in forestry over the six years spanning between the XII World Forestry Congress in Canada and the XIII WFC held in Buenos Aires. The book covers most of the themes of the XIII WFC, from biodiversity through production, policies, environmental services, and economic aspects, linked by sustainability. It provides a comprehensive view of forestry today, conveying its different aspects through one solid piece addressed by authors whose work denotes a concept of sustainable forest management which is not so much a puzzle laboriously put together as a many-faced unity, steered to achieve ultimately a better quality of living for present and future generations.

Insect pest control has continuously evolved from inorganics to botanicals, to chlo- rinated hydrocarbons, to organophosphorus compounds and methylcarbamates, then synthetic pyrethroids and most recently synthetic nicotinoids as the major classes. These insecticides allowed high standards of crop protection at minimal cost. A limitation in each new class of compounds is the selection of resistant strains and ultimate control failures and this serves as a driving force to discover and develop replacement compounds to circumvent resistance and overcome problem areas. The nicotinoids now play a critical role in meeting this need. Three generations of chemicals are involved in the history of nicotinoid insecti- cides. The first generation was the botanical nicotine used for at least three centuries to control sucking insect pests but largely replaced in the 1940s and 1950s by the more effective organophosphorus compounds and methylcarbamates, some with systemic properties. Synthesis programs based on nicotine as a prototype did not yield compounds that could compete with other synthetic insecticides. The second generation was the nitromethylene type such as nithiazine, discovered by Shell sci- entists in a screening/optimization program. The nitromethylenes had the potency, selectivity, and systemic properties but lacked the field effectiveness largely because of photolability (so close yet so far from a major commercial product). The third generation required a series of advances made by Bayer researchers starting from nithiazine as the model and enhancing its photostability and potency with a nitroimine and chloropyridyl moiety, respectively, to give imidacloprid, the subject of much of this monograph.

One of the United States Food and Drug Administration's most difficult tasks is the assessment of risk-benefit ratios for a broad spectrum of therapeutic and prophylactic drugs. Furthermore, it is now widely recog nized that no drug, chemical or even natural substance is completely devoid of risk. Nowhere has this issue been the subject of more controversy than with steroidal contraceptive drugs. Regulated as a special class of products because of their prophylactic use in healthy individuals for prevention of pregnancy rather than for treatment of disease, steroid contraceptives drugs undergo more extensive animal safety tests than any other pharmaceutical agent. This view also contemplates the availability of alternative contraceptive measures posing fewer risks, but the use of less effective methods must take into consideration the hazards associated with pregnancy itself. In April 1983, the Food and Drug Administration and the National Insti tutes of Health in cooperation with the World Health Organization, the Population Council, and the Agency for International Development sponsored a three-day workshop to evaluate current guidelines for preclinical safety studies of contraceptive drugs in light of our extensive knowledge of the side effects of marketed products. The meeting included presenta tions by experts in the fields of comparative metabolism, pharmacokinetics, pharmacology, carcinogenicity, toxicology, coagulation, lipid metabolism, epidemiology and pathology at a meeting of the Fertility and Maternal Health Drugs Advisory Committee of the National Center for Drugs and Biologics, Food and Drug Administration."

In order to produce a superior scholarly treatise in bio medical science, three important conditions need to be met. First, the subject needs to be of recognized importance and preferably one in which a sizeable volume of new knowledge has been added recently. Second, it needs to be quite evident that the field involved re quires much more up-to-date coverage than it has received and third, the choice of the editors and in turn the authors needs to be recognized as outstanding. This major treatise fills these criteria in an admirable way. There are few who would deny the importance of knowledge concerning the circulatory system. This all pervasive system is the route by which virtually all of the cells and tissues of the body receive their nutrition and it is the major route by which metabolic waste products are carried away. Furthermore, the diseases that involve the circulatory system are, by far, the underlying causes of death and morbidity in the largest number of Americans, Western Europeans and several other populations of industrialized nations. Not only is atherosclerosis-induced-ischemic disease of the heart, brain and extremities widespread in these populations but venous occlusive disease also takes a great toll from phlebothrombosis, pulmonary embolism, etc.

One of the most significant challenges facing mankind in the twenty-first century is the development of a sustainable global economy. Within the scientific community, this calls for the development of processes and technologies that will allow the sustainable production of materials from renewable natural resources. Plant material, in particular lignin, is one such resource. During the annual production of about 100 million metric tons of chemical wood pulps worldwide, approximately 45 and 2 million metric tons/year of kraft lignin and lignosulfonates, respectively, are also generated. Although lignosulfonates have found many applications outside the pulp and paper industry, the majority of kraft lignin is being used internally as a low-grade fuel for the kraft pulping operation. A surplus of kraft lignin will become available as kraft mills increase their pulp production without expanding the capacity of their recovery boilers that utilize lignin as a fuel. There is a tremendous opportunity and an enormous economic incentive to find better uses of kraft lignin, lignosulfonates and other industriallignins. The pulp and paper industry not only produces an enormous amount of lignins as by products of chemical wood pulps, but it also utilizes about 10 million metric tons of lignin per year as a component of mechanical wood pulps and papers. Mechanical wood pulps, produced in a yield of 90-98% with the retention of lignin, are mainly used to make low-quality, non-permanent papers such as newsprint and telephone directories because of the light-induced photooxidation of lignin and the yellowing of the papers.

The topics in this volume explore the etiology, cellular mechanisms, epidemiology, genetics, models and potential therapeutic measures for the blinding diseases of retinitis pigmentosa and age-related macular degeneration. Special focus is highlighted in the areas of Mechanisms of Photoreceptor Degeneration and Cell Death (extremely important because very little is known how or why photoreceptors die in these diseases, despite an abundance of genetic information), Age-Related Macular Degeneration (with several novel approaches to its analysis), Usher Syndrome (the most severe form of retinitis pigmentosa, which includes an early or congenital loss of hearing along with blindness), and Gene Therapy. In addition, the section on Basic Science Related to Retinal Degeneration is particularly strong with several laboratories reporting on new discoveries in the area of outer segment phagocytosis, a key component of photoreceptor-retinal pigment epithelial cell interactions in normal and degenerating retinas.

During the course of the developnent of most areas of scien- tific research certain events occur that serve to bring a particular area into focus. We feel that this symposilHll volume serves that role with regard to the study of the reproductive biology of amphib- ians. The collection of vnrks contained in this volume provides an excellent review of a diversity of past contributions to this field of investigation and brings together in one volume the major ap- proaches that have been utilized to study amphibian reproductive biology. The work emphasizes recently-completed studies and therefore can be considered to be an up-to-date account of the field. Equally signif icant, however, the contributors to this volume have excelled in pointing out directions for future research that will be of im- mense value in integrating the component parts of reproductive bi- ology, i.e., genetics, developnental biology, physiology, behavior, and ecology, into an eVOlutionary framework.

In the conflict between logging as a material resource and preservation the forests are becoming depleted throughout the world and this depletion is aggravated by the effects of air pollution. The concept of utilization is thus now being expanded to include vital societal values. These issues are nowhere more acute than in Central and Eastern Europe, where obsolete, poorly maintained industrial plant is common. Air pollutant levels are so high that one area has been called the Black Triangle. Timber is required in this region as a building material and logging for fuelwood is practised, giving rise to a complex linkage between industry, energy supply, deforestation, agriculture, value-added forestry products and economic development. This book presents current information on national and regional issues relating to environmental threats to forest land, focusing on: ecology, environment and science; forest ownership and management; forest product utilization; government and public agency roles. The recommendations, action items and the human network needed for regional-level implementation are detailed in `situation', `position' and `state of the art' papers. The final objective is to develop a plan of action to help abate the negative impacts while sustaining or expanding the forests, the economy and the energy sufficiency of the region.

The authoritative overviews in this volume provide a wealth of practical information on current approaches to the study of insect-plant interactions. Methods described include direct behavioral observation; assays of host finding, oviposition, and feeding behavior of insect herbivores; post-ingestion physiological effects; measurement of food quality and sensory responses of insects to plant stimuli; chemical isolation and identification of active phytochemicals; evaluation of plant resistance to insects; and the biochemistry of allelochemic interactions.

Tree based production systems abound especially in the tropics. Despite the pervasiveness of such multipurpose trees-outside-forest resources, they have not attracted adequate attention in the development paradigms of many nation states. These multispecies production systems impact the ecosystem processes favourably. Yet, our understanding of the diversity attributes and carbon dynamics under agroforestry is not adequate. This book focuses on the role of multispecies production systems involving tree and crop species as a means for carbon sequestration and thereby reduce atmospheric carbon dioxide levels. Sixteen chapters organized into three broad sections titled: Measurement and Estimation, Agrobiodiversity and Tree Management, and Policy and Socioeconomic Aspects represent a cross section of the opportunities and challenges in current research and emerging issues in harnessing carbon sequestration potential of agroforestry systems. "

Fruit flies are enormously important economic pests, as California has learned over the past few years (remember the Mediterranean Fruit Fly?). The problem is expected to get worse, and issues of both basic research and control measures are very important for this pest. This book is the edited, camera-ready proceedings of a recent international symposium on fruit flies of economic importance. It covers current knowledge of fruit fly physiology, genetics, morphology and behavior. It discusses action programs for controlling and using fruit flies in agronomy, as well as the problem of fruit flies in the fruit growing industry.

Nitrogen Fixation by symbiotic organisms is considered an important contribution to the solution of food problems throughout the world. For manyyears, Chinese scientists have focused their research in this area. Today more than half of the total nitrogen fertilizers applied are from biological fixation sources. The editor is an international renowned scientist at the Chinese Academy of sciences. He has brought together contributions from various research fields in China and Europe.Together they present the state-of-the-art in nitrogen-fixation research. The studies range from actino- mycete fixation induced in various genera andspecies of plants, mechanisms and chemical modeling of enzyme systems togenetical engineering of organisms.

The papers in this volume were presented at the Symposium on Steroid Hormone Receptor Systems held October 18-20, 1978, at the Worcester Foundation for Experimental Biology, Shrewsbury, Mass. The meeting was organized to review, discuss, and disseminate new knowledge about the regulation and function of the receptor proteins which mediate estrogen, progestin, glucocorticoid, and androgen action. The symposium brought together leading scientists whose interests span the spectrum of biological organization. On this occasion, Drs. Elwood V. Jensen and Etienne E. Baulieu were honored as recipients of the Tenth Annual Gregory Pincus l1emorial Award for their pioneering studies of steroid hormone receptors. The material covered in this book focuses on the molecular mechanisms which control receptor site availability and function. The topics discussed include recent work on receptor antibodies, antiestrogen action, receptor heterogeneity, nuclear binding and processing of receptor, receptor activation and inactivation mechan isms, interactions between receptor systems, influence of biological rhythms, membrane binding sites, and the evolution of steroid-bind ing proteins. Although studies on steroid receptors have led to the development of new approaches for cancer therapy and contraception, much remains to be discovered in this rapidly expanding field. Hope fully, this book will provide added impetus to the quest for a full understanding of steroid receptor systems by drawing attention to the unresolved questions yet to be answered. Hhile the book is intended primarily for those interested in hormone action, it should be of value to a more general audience including cell, molecular, and developmental biologists."

This book arose from a meeting held at the University of Washington, Seattle, in July of 1986. The meeting was a satellite symposium of the XXXth International Congress of Physiological Sciences which occurred in Vancouver, canada, at that time. 2 Adjustments in the cytoplasmic Ca ] concentration of cells occur in response to a variety of external signals. These fluctuations are a cen tral component of one mechanism by which cells adapt their activities to changes in the external environment and to the requirements of whole body 2 homeostatic mechanisms. It is now clear that redistribution of Ca + within 2 intracellular compartments, as well as changes in the rates of Ca + influx and extrusion at the whole cell level, occur during signal-dependent 2 changes in the cytoplasmic Ca + concentration. In summarizing current research in this area, this volume considers first the properties of indi vidual cation transporting activities located in various cell membranes. It then moves to the cellular level, where the consequence of individual transporting activities acting in concert is examined. l D.phasis is also 2 p1 aced on pa tho1 ogica1 conditions which resu1 t in loss of cell Ca + regu1 a tion as a part of the disease process. We hope that this approach will help the reader to integrate developments in this large and rapdi1y changing fie1 d."