The aim of this Handbook is to cover all fields that are necessary for the management of tropical forest resources. It provides proven and/or promis ing concepts, methods and available comparative data. The exten sive references cited are a valuable source for further in-depth research on specific subjects. The handbook is addressed to forestry professionals, natural re source managers and all those interested in the understanding and hand ling of day-to-day problems of tropical forest resources. It is an attempt to present, in a concentrated form, the vast ex perience gathered in tropical forestry. The available knowledge although far from complete, does not permit us to stand idly by when confronted with the dwindling tropical forests. Excuses not to tackle this problem are not acceptable. The experience of the last decades gives us sufficient background to apply techniques and concepts successfully, to encom pass the complexity of the human and the natural tropical environment. The authors responsible for the respective sections have been recom mended by institutions or individuals specialized in the subject. Our thanks go to aH those unnamed individuals whose dedication and responsible work made this handbook a reality. Our gratitude also to all the colleagues who supported the handbook in its early stages by their firm commitment."

Major and exciting changes have taken place recently in various aspects of bio technology and its applications to forestry. Even more exciting is the prospect of major innovations that the entire field of biotechnology holds for plant growth in general. The importance of these developments for the forestry sector is considerable, particu larly since forestry science has not received the kinds of technical and R&D inputs that, say, agriculture has received in the past few decades. Y ct the problems of defor estation as well as stagnation in yields and productivity of existing forests throughout the world are becoming increasingly apparent, with consequences and ecological ef fects that cause growing worldwide concern. Policies for application of existing knowl edge in biotechnology to the field of forestry and priorities for future research and development are, therefore, of considerable value, because it is only through the adop tion of the right priorities and enlightened policies that scientific developments will move along the right direction, leading to improvements in forestry practices through out the world. It was against this backdrop that the Tata Energy Research Institute (TERI) or ganised a major international workshop on the "Applications of Biotechnology in For estry and Horticulture" at New Delhi in January 1988. The present volume covers the proceedings of this international workshop."

Agricultural, natural resource, and environmental problems are becom ing increasingly interdependent. For example, soil erosion is largely determined by agricultural land use. Both water use and water con tamination depend on land use and technology choice in agriculture. In many areas, the fertilizers and pesticides used in agriculture are ma jor pollutants of ground and surface water, having adverse effects on drinking water and fisheries. Agricultural pollutants such as pesticides also produce adverse health effects for agricultural workers and the consuming public. On the other hand, the availability of water resources and the value of competing land uses influence agricultural production. Additionally, regional air quality problems may affect crops and global environmental trends may have long-term implica tions for farming. Agriculture, natural resources and environmental quality are all heavily regulated in the U. S., but they are done so by a vast array of competing or unrelated agencies within the U. S. Departments of Agriculture, Interior, and Commerce, the Environmental Protection Agency; and numerous state agencies. Considering the large number of bureaucratically remote public agencies involved and the pervasive in terdependencies between agriculture, natural resources and the environ ment, policies develop which are at best uncoordinated and at worst conflicting and counterproductive. These policies have become sources of controversy as different interest groups struggle to affect their im plementation, as different agencies have fought for administrative con trol and as legislative bodies have attempted to enact piecemeal changes."

Genetic engineering through DNA recombinants and the in vitro manipulation of isolated protoplasts has recently attracted much atten- tion in agricultural biotechnology, and has greatly advanced during the last 5 years. In an earlier book, Plant Protoplasts and Genetic Engineer- ing I, methods for the isolation, fusion and culture of protoplasts were reviewed and the regeneration of complete plants from isolated pro- toplasts of rice, potato, soybean, linseed, cabbage, chicory, lettuce, but- terbur, orchids, citrus and some other tree species, and interspecific and intergeneric somatic hybrids in Lycopersicon, Petunia, Nicotiana, Solanum, Glycine, Citrus, Brassica, Medicago and Trifolium spp. were discussed. The present volume, Plant Protoplasts and Genetic Engineering II, deals with some of the newer techniques such as microinjections, elec- trofusion, flow cytometry, uptake and integration of DNA, nuclei, iso- lated chromosomes by plant protoplasts and the subsequent regeneration of transgenic plants. The literature on the DNA recombinants and genetic transformation, both Agrobacterium-mediated and direct gene transfer in agricultural crops and trees, such as poplars, is reviewed, and the uses of cytoplasts and miniprotoplasts in genetic manipulation are highlighted.

The aim of this book is to provide an accessible overview for advanced students, resource professionals such as land managers, and policy makers to acquaint themselves with the established science, management practices and policies that facilitate sequestration and allow for the storage of carbon in forests. The book has value to the reader to better understand: a) carbon science and management of forests and wood products; b) the underlying social mechanisms of deforestation; and c) the policy options in order to formulate a cohesive strategy for implementing forest carbon projects and ultimately reducing emissions from forest land use.

Provided here are both underlying theory and recent results concerning the propagation and use of clones in research and in production forestry. State-of-the-art science and case histories treating production, testing, multiplication and deployment of clones are presented. Agroforestry, urban forestry and christmas-tree farming are covered, along with more traditional multiple-use forestry and high-intensity forestry for biomass, wood and fiber production. Clonal forestry is contrasted to the more recent developments of "family forestry," and the classical tree-improvement approach relying on seed-orchards. The history of clonal forestry is covered with reviews of several centuries experience with Sugi in Japan and poplars in Europe. The impacts and use of clones in the contexts of genetic conservation and biodiversity are discussed, as are the laws and regulations affecting clonal production and deployment.

Wild taxa are invaluable sources of resistance to diseases, insects/ pests, nematodes, temperature extremes, salinity and alkalinity stresses, and also of nutritional quality; adaptation; genetic diversity and new species. Utilization of wild relatives of a crop depends largely upon its crossability relations with cultivated varieties. Sev eral wild species are not crossable with the commercial cultivars due to various isolation barriers. Furthermore, in a few cases, hybridiza tion is possible only in one direction and reciprocal crosses are not successful, thus depriving the utilization of desired cytoplasm of many species. However, techniques have been developed to over come many barriers and hybrid plants are produced. New crop species have been developed by overcoming the F 1 sterility and producing amphidiploids and such crops are commercially being grown in the field. The segregation pattern ofF 1 hybrids produced by distant hybridization in segregating generations are different from the intervarietal hybrids. In former cases, generally, unidirectional segregation takes place in early generations and accordingly, selec tion procedures are adopted. In most of the cases, backcross or modified backcross methods have been followed to utilize wild species, and thus numerous types of resistance and other economical attributes have been transferred in the recurrent parents. Protoplast fusion has been amply demonstrated in a number of cases where sexual hybridization was not possible and, as a result, hybrids have been produced."

T. C. Hutchinson The NATO Advanced Research Workshop detailed in this volume was held in Toronto, Canada, in 1985. The purpose of the Workshop was to provide a "state of the art" report on our knowledge of the sensitivities and responses of forests, wetlands and crops to airborne pollutants. Approximately 40 scientific experts from nine countries participated. Most participants were actively involved in research concerning the effects of air pollutants on natural or agro-ecosystems. These pollutants included acidic deposition, heavy metal particulates, sulphur dioxide, ozone, nitrogen oxides, acid fogs and mixtures of these. Also invited were experts on various types of ecosystem stresses, physiologi cal mechanisms pertinent to acid deposition, and other areas that were felt by the director to be of direct relevance, including: effects of ethylene on vegetation, the physiology of drought in trees, the nature and role of plant cuticles as barriers to acid rain penetration, the use of dendrochronological techniques in reconstructing the time of onset and the subsequent progression of growth declines, the ability of soils to naturally generate acidity, the role of Sphagnum moss in natural peat land acidity, the use of lichens as indicators of changing air quality, and the magnitude of natural emissions of reduced sulphur gases from tropical rainforests and temperate deciduous forests. The Workshop included a series of invited presentations and subsequent group discussions. These presentations were designed to allow syntheses of our present knowledge as well as detailed questioning and discussion."

Can a continuous growth of agriculture be achieved in the sub-Sahara region without inducing irreversible damage to the ecosystem? Until now, doubts have been expressed as to the actual capacity of the soils to sustain a desirable increase of production which can match the requirements of a fast-growing population. Thirty years of investigation and a renewed comprehensive interpretation of research data on soil fertility show that a sustainable agriculture growth could be a practical possibility in a savannah region.

This book provides a quantitative analysis of the role of woody plants in semi-arid regions, for the aSSessment of their benefits in agrosylvopastoralland-use systems with productive and sus tainability objectives. The insights presented and conclusions drawn allow the additional benefits of woody plants for specific climatic and physical site conditions and land-use systems to be estimated. The Sahel and Sudan zones in West Africa, on which the book focusses, represent resource-poor conditions, whose ecological dynamics have been relatively well studied. The role of woody plants in this region, as assessed in this book, is extrapolated to other semi-arid regions, leading to general conclusions on agroforestry's potential as an option for sustainable land use in semi-arid regions. The origins of this book go back to 1982, when the Club du Sahel requested that available data on woody plants in the Sahel region be synthesised, to provide basic information to enable better attention to be given to woody plants in rural development programmes. We are grateful to the Club du Sahel for this challenge. Various people contributed to studies used in this book. The preliminary inventory of the data available was made by Frits Ohler; later his work was continued by Franciska Dekker."

Haploid plants have the gametophytic number of chromosomes. They are of great importance, especially in studies on the induction of muta tions and also for the production of homozygous plants, they are needed in large numbers. The conventional methods employed by plant breeders for their production are cumbersome, time-consuming, laborious and rather inefficient. Sometimes it may take years to produce a pure line. However, with the introduction of in vitro techniques, especially anther culture for the induction of androgenesis, it has become increasingly evi dent that these methods considerably accelerate the production of haploids for plant breeding programs. During the last decade, in vitro-produced haploids have been incor porated into breeding programs of many agricultural crops, and positive results have been obtained especially with rice, wheat, potato, barley, maize, asparagus, sunflower, brassica, tobacco, etc. Among these, rice and wheat are the best examples in which a number of improved varieties have been released. In wheat, the breeding cycle can be shortened by three or four generations when the pollen haploid breeding method is used instead of conventional cross-breeding. The release of the wheat varieties Jinghua 1 and Florin is a typical example of what can be achieved with other crops. Taking these developments into considera tion, the present volume, Haploids in Crop Improvement I, was compil ed.

Most forest tree species were considered recalcitrant a decade ago, but now with the improved in vitro techniques some progress has been made towards culture-of tree species. Micro propagation has been achieved from the juvenile tissues of a number of forest tree species. On the other hand, tissues from most mature trees are still very difficult to grow and differen tiate in vitro. Nevertheless, there has been slow but steady progress in the application of tissue culture technology for culture of tissues, organs, cells and protoplasts of tree species. As compared to most agricultural crops, and herbaceous plant species, trees are a different lot. They have long gene ration cycles. They are highly heterozygous and have a large reservoir of genetic variability. Because of this genetic variability, their response in vitro is also variable. On a single medium, the response of tissues from different trees (genotypes) of a single species may be quite different: some responding by induction of growth and differentiation, while others showing minimal or no growth at all. That makes the somatic cell genetics of woody plants somewhat difficult, but at the same time interesting."

With the emergence of urban and community forestry as the fastest growing part of our pro fession in the last 15 years, the need for a book such as this inevitably developed. The So ciety of American Foresters' urban forestry working group counts 32 or more universities now offering courses in this subject, and the number is growing. For the last several years I have coordinated a continuing education urban forestry course at Rutgers for nonmatriculated students. Registrants have included arborists, shade tree commissioners, landscape architects, city foresters, environmental commissioners, park superintendents, and others whose jobs involve care and management of trees. The course was started by Bob Tate in 1980, around a core of managerial subjects such as in ventories, budgets, and public relations. After Bob left in 1984 to join Asplundh and later to start his own prosperous business in California, the course languished after it exhausted the local market for those subjects.

Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis, was first introduced in 1954, the considerations were: 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; - 2. the difficulty in finding many new analytical methods in specialized journals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes to incomplete, that it is difficult to reproduce experiments. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be just as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for the success of any publication, but we believe that the methods published in the first series were up-to-date at the time and presented in a way that made description, as applied to plant material, complete in itself with little need to consult other publications. Contribution authors have attempted to follow these guidelines in this New Series of volumes. Editorial The earlier series of Modern Methods of Plant Analysis was initiated by Michel v.

Preservation of nature and the environment has become one of the most important issues of the end of the twentieth century. It has become evident that the methods used for industrial and agricultural production in many countries produce pollutants that cannot undergo natural neutralization by entering the atmosphere, soil or water. Ecosystems that have been developing for centuries are undergoing degradation and what is even more regrettable is that there is an actual threat of profound disorder in the biosphere which could lead to heavy and irreversible changes. Fluorine derivatives are the most aggressive among toxic compounds polluting the atmosphere. Moreover, the percentage of fluorides in industrial emissions is constantly increasing with the bulk of fluorides being emitted by aluminium smelters. Fluorine is poorly detoxified by both plants and animals and the accumulation of even relatively low concentrations over a long period causes a cumulative toxic effect. Among woody plants conifers are less resistant to fluorine. Fluorine derivatives as phytopollutants have been studied less than sulphur compounds, nitrogen oxides, chlorine and hydrogen chloride. It was not until the late 1960s when there was a rapid decline of coniferous forests that researchers directed their attention towards phyto toxic properties of fluorides."

After the 1988 and 1989 volumes, this is the third volume on Medicinal and Aromatic Plants. Each of the 29 chapters contributed by international scientists deals with one individual plant genus, namely "Atropa," "Ageratina," "Ailanthus," "Aconitum," "Apium," "Aloe," "Akebia," "Bidens," "Carthamus," "Chamomilla," "Carum," "Citrus," "Cymbopogon," "Dysosma," "Euphorbia," "Fritillaria," "Glycyrrhiza," "Lavandula," "Nigella," "Pelargonium," "Perilla," "Podophyllum," "Rosa," "Scutellaria," "Securinega," "Solanum," "Swertia," "Symphytum," "Syringa." Their distribution, economic importance, conventional propagation, in-vitro propagation and production of metabolites through tissue culture are treated in detail. Special emphasis is laid on the potential of industrial in-vitro production of plant compounds of medical and pharmaceutical relevance using tissue culture.

Nitrogen is a key element in ecosystem processes. Aspects of local and global changes in nitrogen in both undisturbed and disturbed conditions are discussed. Environmental changes caused by pollution from nitrogenous compounds and changes in landuse are also described. Organisms, plants, animals and microorganisms are all affecting nitrogen supply. Emphasis is placed on natural and anthropogenic transfer of nitrogen between ecosystems and also on the interaction of nitrogen with other bioelements.

This volume of Advances in Microbial Ecology marks a change in the editor ship of the series. The Editorial Board wishes to take this opportunity to express its gratitude to Martin Alexander, the founding editor and editor of the first five volumes, for his enterprise in establishing the series and in ensuring that Advances has become an outstanding focal point for the identification of new developments in the rapidly expanding field of microbial ecology. With the publication of this volume, we welcome Howard Slater to the Editorial Board. The policies of the Editorial Board remain the same as before. Most con tributions to Advances in Microbial Ecology will be solicited by the Board. However, individuals are encouraged to submit outlines of unsolicited contri butions to the Board for consideration for inclusion in the series. Advances is designed to serve an international audience and to provide critical reviews on basic and applied aspects of microbial ecology. Contributions in the present volume are predominantly concerned with the ecology of aquatic microorganisms, but encompass a variety of approaches to this area. The exception is the chapter by J. W. Doran on the role of micro organisms in the cycling of selenium. G-Y. Rhee discusses the effects of envi ronmental factors on phytoplankton growth. The factors limiting the produc tivity of freshwater microbial ecosystems are considered by H. W. Paerl."

th th On June 17 and 18 1987 the fourth workshop on "Azospi rillum: Genetics, Physiology, Ecology" took place at the Uni ver sity of Bayreuth, West Germany, organized by the Genetics depart ment. There were about 80 participants, who came from German research institutions, from other European countries, from Is rael, Egypt, India, North Vietnam, and North and South America. The former workshops had also taken place in Bayreuth, in 1981, 1983 and 1985, hence the organizers could draw on the experience obtained at these earlier workshops. Azospirilla have, during the past 15 years, found an ever increasing scientific interest because, first, these soil bacte ria carry the genetic information for binding molecular nitrogen from the air and, second, they live in close vicinity to the roots of grain crops and forage grasses. By exploitation of these two properties, it is hoped to develop inoculation procedures which can be used in nitrogen-deficient soils.

As J understand it, a book Preface is where the author explains to the reader how the book in hand came about, something of the personal reasons for having inflicted such extended duress on one's self to complete the manllscript. and other items that are fit to say but do not fit in the text. This book had its conceptual beginnings in the 1970's wit h my 'studies in scientific synthesis at the North Central Forest Experiment Station, St. Paul, Minnesota. Ours is, clearly, the age of analysis. But, I felt, we must soon begin frameworks for synthesis, or a synthesis would never be possible. In short, I hoped to develop 'interaction' as an integrative principle in forestry. As work progressed on the manuscript, other subthemes developed. First, there was the vague feeling on my part that the forestry profession was losing ground in the contest to see who should manage the forests of the world. This was happening not because foresters do not know how to manage forests in a reasonable manner, but because the public seemed to be loosing faith in the judgement of foresters as professional, responsible, wise land managers. Several well-known incidents of poor judgement in timber harvesting methods on national forests in the United States did little to help the forester's image.

The esculent Lycopersicon esculentum, long thought to be poisonous, has become a major U. S. food crop and source of vitamins and minerals, thanks largely to genetic modification and new production technology Rick (1978) Tomato (Lycopersicon esculentum Mill. ) is one of the most important solana ceous vegetable crops grown worldwide under outdoor and indoor conditions. It has become an important commercial crop so far as the area, production, industrial values and its contribution to human nutrition is concerned. During the past few decades tremendous developments have contributed to the knowledge and understanding of various areas of genetics, breeding and biotechnology and voluminous literature has been generated. The purpose of preparing this monograph is to give a comprehensive up-to-date treatment to the various aspects of genetic improvement of tomato. The emphasis has been placed on cytology, classical and molecular genetics, reproductive biology, germplasm resources, hybrid seed production, use of wild taxa, selection/ breeding methods, breeding for abiotic and biotic stresses, processing and quality breeding, improvement for mechanical harvesting, and biotechnology: tissue culture, protoplast fusion, and genetic transformation. These topics are presented in 22 different chapters. However, a few aspects have been discussed in more than one chapter. For example, seed production is treated in chapters 1, 4 and 8; molecular biology/genetic engineering in chapters 3 and 22 and heterosis in chapters 8 and 16."

Fertilization in mammals normally occUrs within the oviduct, where it is relatively inaccessible to study. However, as a result of painstaking research, most of it carried out over the last five years, this barrier to experimentation has been largely overcome by the development of in vitro fertilization techniques for at least 11 different species, including man. The result has been a rapid increase in our knowledge of the physiological and bio chemical mechanisms involved in the fertilization process. The aim of this book, which is an extension of my recent review of cell surface interactions in fertilization (Gwatkin, 1976), is to present a brief, but well documented, account of the new knowledge that has been attained. Although this book deals with mammalian fertilization mechanisms, I have included some recent experiments on am phibian and invertebrate gametes to supplement the mammalian picture. This information is particularly valuable as the rela tively large number of eggs available from these lower forms has advanced our knowledge of certain fertilization mechanisms beyond what is known in mammals. However, in the interest of brevity, I have omitted details of morphology and minor varia tions between species. For these, and other aspects not covered here, the reader is referred to the books of Austin (1965, 1968), vii viii PREFACE Austin and Short (1972), Metz and Monroy (1969), Monroy (1965), Lord Rothschild (1956a), and Zamboni (l971a)."

This proceedings is based on a joint meeting of the two IUFRO (International Union of Forestry Research Organizations) Working Parties, Somatic Cell Genetics (S2.04-07) and Molecular Genetics (S2.04-06) held in Gent, Belgium, 26-30 September, 1995. Although a joint meeting of the two Working Parties had been discussed in the past, this was the first such meeting that became a successful reality. In fact this meeting provided an excellent forum for discussions and interactions in forest bioteclUlology that encouraged the participants to vote for a next joint meeting. In the past decade rapid progress has been made in the somatic cell genetics and molecular genetics of forest trees. In order to cover recent developments in the broad area of biotechnology, the scientific program of the meeting was divided into several sessions. These included somatic embryogenesis, regeneration, transformation, gene expression, molecular markers, genome mapping, and biotic and abiotic stresses. The regeneration of plants, produced by organogenesis or somatic embryogenesis, is necessary not only for mass cloning of forest trees, but also for its application in genetic transformation and molecular biology. Although micropropagation has been achieved from juvenile tissues in a number of forest tree species, in vitro regeneration from mature trees remains a challenging problem in most hardwoods and conifers. The mechanisms involved in the transition from juvenile to mature phase in woody plants are poorly understood. This transition can now be investigated at the molecular level.

r-------------{ Environment (Disease) Fig. 1. A schematic presentation of the interplay between the external environment, pathogen and animal, which influences resistance to infectious disease. Disturbance in equilibrium results in infection and disease skin and the mucous membranes of the respiratory tract. These tissues are in contact with the environment, and direct injury to them facilitate entry of pathogenic microorganisms through these important natural barriers. Sunburn and frostbite are examples of such adverse effects. Climatic factors such as heat and cold may also act as physiological stress factors which affect the specific and non-specific responses of the body to infection. 1.1.2 Pathogen Survival Climatic factors may affect dispersal, spread and survival of pathogenic micro organisms in the environment. This is also true for arthropod vectors such as mosquitos and ticks (Smith 1970; Ferguson and Branagan 1972). The density of the animal population is an important factor determining the concentration of patho gens in the environment. Population density can be influenced by weather condi tions, as animals respond to heat and cold by typical changes in behaviour. For example, in cold weather they tend to huddle together. This behaviour results in increased population density, which in turn involves an increased risk of the spread of airborne infections."

The rapidly growing human population has increased the dependence on fossil fuel-based agrochemicals, such as fertilizers and pesticides, to produce the required agricultural and forestry products. This has exerted great pressure on non-renewable fossil fuel resources, which cannot last indefinitely. Not only do agrochemicals pollute the environment, but pests also become resistant to pesticides. Thus, present agricultural practices exploit natural resources, and damage fauna and flora and agroecosystems. One safe alternative to overcome these problems is the use of allelopathy to sustain development in agriculture and forestry and maintain a clean environment for future generations. This book is the Proceedings of the III International Congress on Allelopathy in Ecological Agriculture and Forestry, held on August 18-21, 1998, at the University of Agricultural Sciences, Dharwad, Karnataka, India, and provides an updated status of current allelopathy research in various leading countries, with the overall aim of developing new technologies for ecological agriculture and forestry in the 21st century. To date, no book on ecological agriculture has discussed these aspects, hence it is the first time that such information is available. The chapter contributors are leading specialists in their fields, and all chapters have been peer-reviewed by international referees. This book will be indispensable for agricultural scientists (agronomists, entomologists, nematologists, plant pathologists, horticulturists, plant breeders, agroforesters, foresters, soil scientists), bioscientists (biochemists, organic chemists, plant ecologists, microbiologists and limnologists), environmentalists, graduate students and farmers, as well as for organizations engaged in sustainable agriculture and organic agriculture.