The chapters in this volume are based on a opportumtles for studying the links between symposium, "California grasslands: structure abiotic and biotic components. and productivity," supported by the National The contributions in this volume illustrate Science Foundation. The primary objective of the links between population-level processes this symposium was to integrate the current and system-level phenomena in a well-studied understanding of controls on ecosystem struc community. Unfortunately, some areas of cur ture and function with the approaches of popu rent research (e.g., nutrient cycling) are under lation biology. The annual grasslands are represented in this volume. For other topics eminently suitable for experimental and manip (particularly the role of invertebrate con sumers), the lack of data from the annual grass ulative studies of ecosystem processes. The short lives and small stature of the component land brought a broader grassland perspective. plant species make experimental work far more Together, however, the contributions illustrate practical than in forests or even in perennial the importance of different ecological ap dominated prairies. The system's small-scale proaches in studying the controls on structure patchiness, and the obvious importance of and function of a complex system. the region's mediterranean climate in the life cycle of the annual vegetation, afford many L.F. Huenneke and H.A. Mooney Huenneke, L.F. and Mooney, H. (eds) Grassland Structure and Function: California Annual Grassland."

This volume is developed on the broad theme of plant-associated bacteria. It is envisioned as a resource volume for researchers working with beneficial and harmful groups of bacteria associated with crop plants. The book is divided into two parts. Part I (9 chapters) on beneficial bacteria includes chapters on symbiotic nitrogen fixers, diazotrophs, epiphytes, endophytes and rhizosphere bacteria and deleterious rhizobacteria. Part II (8 chapters) consists of detailed descriptions of 8 genera of plant pathogenic bacteria: Agrobacterium, Clavibacter, soft-rot Erwinia, Pseudomonas, Xanthomonas, Ralstonia, Burkholderia and Acidovorax and Herbaspirillum. There is an opening chapter on the plant-associated bacteria survey, molecular phylogeny, genomics and recent advances. And each chapter includes terminology/definitions, molecular phylogeny, methods that can be used (both traditional and latest molecular tools) and applications.

Invasive plants have an impact on global biodiversity and ecosystem function, and their management is a complex task.

The aim of this book is to discuss fundamental questions of invasion ecology, such as why particular communities become more invasible than others, what the mechanisms of exclusion of native species by invaders are, and whether invasion can be predicted.
In addition, agricultural practices influencing invasion, the environmental and economic costs of invasion as well as possible management strategies are discussed. Readers will get a unique perspective on invasion ecology through employing general principles of ecology to plant invasions.

This book provides up-to-date knowledge of the promising field of Nanobiotechnology with emphasis on the mitigation approaches to combat plant abiotic stress factors, including drought, salinity, waterlog, temperature extremes, mineral nutrients, and heavy metals. These factors adversely affect the growth as well as yield of crop plants worldwide, especially under the global climate change. Nanobiotechnology is viewed to revolutionize crop productivity in future. The chapters discuss the status and prospects of this cutting-edge technology toward understanding tolerance mechanisms, including signaling molecules and enzymes regulation in addition to the applications of Nanobiotechnology to combat individual abiotic stress factors.

While there are a few plant cell biology books that are currently available, these are expensive, methods-oriented monographs. The present volume is a textbook for "upper" undergraduate and beginning graduate students." This textbook stresses concepts and is inquiry-oriented. To this end, there is extensive use of original research literature. As we live in an era of literature explosion, one must be selective. These judgements will naturally vary with each investigator. Input was sought from colleagues in deciding the literature to include. In addition to provision of select research literature, this volume presents citations and summaries of certain laboratory methods. In this connection, the textbook stresses quantitative data to enhance the student?s analytical abilities. Thus the volume contains computer-spread sheets and references to statistical packages, e.g. Harvard Graphics and Statistica.

'Biopiracy' refers either to the unauthorized extraction of biological resources, such as plants with medicinal properties, and associated traditional knowledge from indigenous peoples and local communities, or to the patenting of spurious 'inventions' based on such knowledge or resources without compensation. Biopiracy cases continue to emerge in the media and public eye, yet they remain the source of considerable disagreement, confusion, controversy and grief. The aim of this book is to provide the most detailed, coherent analysis of the issue of biopiracy to date.

The book synthesizes the rise of the issue and increasing use of the term by activists and negotiators in the World Trade Organization (WTO) and the Convention on Biological Diversity (CBD), to form a critical understanding of the themes, implications and politics of biopiracy. Taking a case-study based approach, derived from interviews and fieldwork with researchers, government, industry, local farmers, healers and indigenous people, the author sequentially documents events that have occurred in biopiracy and bioprospecting controversies. Implications and ethical dilemmas are explored, particularly relating to work with local communities, and the power relations entailed. Detailing international debates from the WTO, CBD and other fora in an accessible manner, the book provides a unique overview of current institutional limitations and suggests ways forward. Options and solutions are suggested which are relevant for local communities, national governments, international negotiators, NGO and interest groups, researchers and industry.

Maize is a globally important crop mainly utilized as feed, food and raw material for diverse industrial applications. Among cereals, it occupies third place after rice and wheat and is a staple food for a large segment of population worldwide particularly in the Asian as well as African countries. This monogram discusses various aspects of nutritional quality of maize such as quality protein maize which has been considered as most significant discovery in enhancing nutritional quality of cereals in terms of increasing the concentration of essential amino acids. The biochemistry of starch which is an important industrial product of maize has been discussed in detail. Further, the role of maize oil which is highly regarded for human consumption as it reduces the blood cholesterol concentration has also been elaborated. Naturally, maize is a rich source of carotenoids such as beta-carotene, zeaxanthin, lutein, cryptoxanthin which have highly diverse health benefits ranging from maintaining normal vision to lowering of oxidative stress. The need for biofortification of maize for provitamin A carotenoids and their role in alleviating vision impairments have also been discussed. The effect of various biotic and abiotic stresses particularly carbon dioxide and temperature on quality has been discussed thoroughly. Many value-added products as well as fermented foods that have been produced from maize which is consumed in different forms worldwide are also discussed. The aspects related to the maize application as fodder and as a source of malting have also been covered concisely. Overall, the book provides complete information about various quality aspects of maize. The various stakeholders such as maize researchers, extension specialists, students, teachers as well as farmers will be immensely benefitted from this monogram.

The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapters 19 and 20) but also from blue native 1D gels (Chapters 27 and 28) despite the fact that Edman sequencing can still be useful (Chapter 18). Associated with other techniques such as 2DLC or LC of intact proteins, mass spectrometry also permits the identification of polypeptides from complexes (Chapters 21 and 22).

'frees contribute a major part of fuel, fodder and fruit, and are an im of bioenergy. They are now needed in large numbers more portant source than ever before for afforestation and social forestry, so that fast-grow ing and multipurpose trees assume great importance. After extensive in discriminate deforestation and rapid depletion of genetic stocks, efforts are now being made to evolve methods for clonal mass propagation of improved and elite trees. Production of short-duration trees with a rapid turnover of biomass, and induction of genetic variability through in vitro manipulation for the production of novel fruit and forest trees, which are high-yielding and resistant to pests and diseases, and trees which display increased photosynthetic efficiency are in demand. These objectives are well within the realm of horticultural and forest biotech nology. Some of the recent advances, such as the regeneration of com plete trees from isolated protoplasts, somatic hybridization, and the Agrobacterium-mediated transformation in various tree species have opened new vistas for the genetic engineering of fruit and forest trees. This book is a continuation of the earlier volume Trees I, and presents 31 chapters on fruit, forest, nut and ornamental trees, such as avocado, pineapple, crabapple, quince, pistachio, walnut, hazelnut, date palm, oil palm, cacao, rubber, maple, sweet-gum, poplars, birches, Chinese tallow, willows, oaks, paper mulberry, rhododendrons, Scots pine, Calabrian pine, Douglas-fir, redwood, ginkgo, cycads and some flowering trees."

This book presents basic concepts, methodologies and applications of biotechnology for the conservation and propagation of aromatic, medicinal and other economic plants. It caters to the needs and challenges of researchers in plant biology, biotechnology, the medical sciences, pharmaceutical biotechnology and pharmacology areas by providing an accessible and cost-effective practical approach to micro-propagation and conservation strategies for plant species. It also includes illustrations describing a complete documentation of the results and research into particular plant species conducted by the authors over the past 5 years. Plant Biotechnology has been a subject of academic interest for a considerable time. In recent years, it has also become a useful tool in agriculture and medicine, as well as a popular area of biological research. Current economic growth is globally projected in a highly positive manner, but the challenges many countries face with regard to food, feed, malnutrition, infectious diseases, the newly identified life-style diseases, and energy shortages, all of which are worsened by an ever-deteriorating environment, continue to pull the growth digits back. The common thread that connects all of the above challenges is biotechnology, which could provide many answers. Molecular biology and biotechnology have now become an integral part of tissue culture research. The tremendous impact generated by genetic engineering and consequently of transgenics now allows us to manipulate plant genomes at will. There has indeed been a rapid development in this area with major successes in both developed and developing countries. The book introduces several new and exciting areas to researchers who are unfamiliar with plant biotechnology and also serves as a review of ongoing research and future directions for scholars. The book highlights numerous methods for in vitro propagation and utilization of techniques in raising transgenics to help readers reproduce the experiments discussed.

Mitogen-activated protein kinases (MAPKs) are versatile phosphorylating enzymes which regulate multiple proteins involved in gene expression, cell architecture, plant development and reaction to diverse abiotic and biotic factors. The main aim of Plant MAP Kinases: Methods and Protocols is to provide established and new MAPK protocols adapted to the challenges posed by working with plants. The book contains 19 chapters which encompass a wide array of methods progressively scaling from the single gene, protein or cell level to large-scale arrays of proteomic, phosphoproteomic and interactomic data in order to uncover previously unidentified plant MAPK signaling pathways and to tackle with the challenging task of substrate identification. Techniques for MAPK sequence analysis and subcellular localization helping to identify their substrates and subcellular compartmentalization are also provided.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant MAP Kinases: Methods and Protocols represents a collection of useful plant MAPK protocols written by experts in the field for researchers and students.

This treatise is focused on early aspects of fungal pathogenesis in plant and animal hosts. Our aim in choosing the topics and contributors was to demonstrate common approaches to studies of fungal-plant and fungal-animal interactions, particularly at the biochemical and molecular Ievels. For example, the initial events of adh"sion of fungal spores to the exposed surface tissues of the host are essential for subsequent invasion of the plant or animal and establishment of pathogenesis. A point of consensus among investigators who have directed their attention to such events in plants, insects, and vertebrates isthat spore adhesion to the host cuticle or epithelium is more than a simple binding event. lt is a complex and potentially pivotal process in fungal-plant interactions which "may involve the secretion of ftuids that prepare the infection court for the development of morphological stages of the germling" and subsequent invasion of the host (Nicholson and Epstein, Chapter 1). The attachment of the fungal propagule to the arthropod cuticle is also "mediated by the chemical components present on the outer layer of the spore wall and the epicuticle . . . . Initial attachment may be reinforced further by either the active secretion of adhesive materials or the modification of spore wall materiallocated at the [fungal spore arthropod] cuticle interface (Boucias and Pendland, Chapter 5).

Alan Powderham's dazzling photography is complemented by Dr Sancia van der Meij's scientific expertise, creating a scientifically rigorous yet breathtakingly beautiful reference work. Van der Meij is an experienced coral reef specialist while Powderham's use of rebreather diving technology allows him to get unusually close to his subject matter. The spectacular landscapes and natural riches of the Coral Triangle are under threat from overfishing, climate change, unsustainable tourism, habitat destruction and poor governance. This book showcases exactly why we need to protect this unique ecosystem, contrasting its existing beauty with images of the damage already being perpetrated. 10% of the author royalties are going to Conservation International to aid their work. The book covers many of the groups of animals seen on a reef that have been largely omitted by others. It reports engaging observations giving insights to many unique animal behaviours and relationships, that make it stand out from other books.

Biotic stresses cause yield loss of 31-42% in crops in addition to 6-20% during post-harvest stage. Understanding interaction of crop plants to the biotic stresses caused by insects, bacteria, fungi, viruses, and oomycetes, etc. is important to develop resistant crop varieties. Knowledge on the advanced genetic and genomic crop improvement strategies including molecular breeding, transgenics, genomic-assisted breeding and the recently emerging genome editing for developing resistant varieties in technical crops is imperative for addressing FHEE (food, health, energy and environment) security. Whole genome sequencing of these crops followed by genotyping-by-sequencing have facilitated precise information about the genes conferring resistance useful for gene discovery, allele mining and shuttle breeding which in turn opened up the scope for 'designing' crop genomes with resistance to biotic stresses. The 15 chapters dedicated to 13 technical crops and 2 technical crop groups in this volume will deliberate on different types of biotic stress agents and their effects on and interaction with crop plants; will enumerate on the available genetic diversity with regard to biotic stress resistance among available cultivars; illuminate on the potential gene pools for utilization in interspecific gene transfer; will brief on the classical genetics of stress resistance and traditional breeding for transferring them to their cultivated counterparts; will enunciate the success stories of genetic engineering for developing biotic stress resistant varieties; will discuss on molecular mapping of genes and QTLs underlying biotic stress resistance and their marker-assisted introgression into elite varieties; will enunciate on different emerging genomics-aided techniques including genomic selection, allele mining, gene discovery and gene pyramiding for developing resistant crop varieties with higher quantity and quality; and will also elaborate some case studies on genome editing focusing on specific genes for generating disease and insect resistant crops.

This book Trees IV, like the previous volumes (Trees I, II, III published in 1986, 1989, 1991, respectively), is special in its approach. It elucidates the case history and biotechnology of individual fruit, forest, and ornamental trees, and discusses the present state of the art, with particular reference to in vitro propagation. It comprises 24 chapters contributed by international experts, and deals with the importance, distribution, conventional propa gation, micropropagation, review of tissue culture studies, and recent advances in the in vitro culture and genetic manipulation of various species of Acrocomia, Ailanthus, Anacardium, Allocasuarina, Carya, Casuarina, Coffea, Cyphomandra, Feijoa, Fraxinus, Gymnocladus, Leptospermum, Fagus, Metroxylon, Oxydendrum, Paeonia, Paulownia, Pouteria, Psidium, and Quercus. Included are also five chapters on gymnosperm trees, such as Abies jraseri, Cephalotaxus harringtonia, Pinus durangensis, P. gregg ii, P. halepensis, P. pinea, and Tetraclinis articulata. Trees IV is a valuable reference book for scientists, teachers, and students of forestry, botany, genetics, and horticulture, and all those who are interested in the biotechnology of trees. New Delhi, March 1996 Professor y. P. S. BAJAJ Series Editor Contents Section I Angiosperm Trees 1. 1 Acrocomia Species (Macauba Palm) O. l. CROCOMO and M. MELO (With 8 Figures) 1 General Account . . . . . . . . . . 3 2 Chemical Composition . . . . . . 5 3 Genetics and Crop Improvement 9 4 In Vitro Culture Studies 10 5 Industrial Utilization . . . 14 6 Lauric Acid . . . . . . . . . 15 7 Summary and Conclusions 15 References . . . . . . . . . . . . . . . . . . . . . . . . . 16 1. 2 Ailanthus altissima Mill. Swingle (Tree of Heaven) M. ZENKTELER and B."

Innovative Strategies for Teaching in the Plant Sciences focuses on innovative ways in which educators can enrich the plant science content being taught in universities and secondary schools. Drawing on contributions from scholars around the world, various methods of teaching plant science is demonstrated. Specifically, core concepts from ethnobotany can be used to foster the development of connections between students, their environment, and other cultures around the world. Furthermore, the volume presents different ways to incorporate local methods and technology into a hands-on approach to teaching and learning in the plant sciences. Written by leaders in the field, Innovative Strategies for Teaching in the Plant Sciences is a valuable resource for teachers and graduate students in the plant sciences.

This volume explores the latest advances in high-throughput phenotyping to screen milling quality, cooking and nutritional quality in breeding material, and simplifying grain quality screening in germplasm. The chapters in this book cover topics such as seed development stages; panicle architectural traits to understand yield components; starch structure and protein properties; and genome editing technologies to reveal grain quality and nutritional traits. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Rice Grain Quality: Methods and Protocols is a valuable resource for all researchers interested in expanding their knowledge in this evolving field.

Topics covered in this book include RNA silencing and its suppression in plant virus infection, virus replication mechanisms, the association of cellular membranes with virus replication and movement, plant genetic resistance to viruses, viral cell-to-cell spread, long distance movement in plants, virus induced ER stress, virus diversity and evolution, virus-vector interactions, cross protection, geminiviruses, negative strand RNA viruses, viroids, and the diagnosis of plant viral diseases using next generation sequencing. This book was anticipated to help plant pathologists, scholars, professors, teachers and advanced students in the field with a comprehensive state-of-the-art knowledge of the subject.

Discover the fascinating stories behind 300 species of fungi and understand the world of mushrooms like never before! Did you know that fungi put the fizz in champagne and the flavour in chocolate? Fungi is everywhere we look: in a forest, under the sea, and in the kitchen. In this beautiful book, leading fungal biologists Lynne Boddy and Ali Ashby bring you closer to 300 species of mushrooms and lichens through fascinating facts, mushroom datasets, and detailed illustrations. Discover some of the fastest speeds in nature, specimens that glow in the dark, and fungi that clean up oil spills. Humans have had a close relationship with mushrooms for thousands of years - from using the shiitake for healing, to telling stories of enchanted fairy rings, and cooking gourmet dishes with rare specimens. Bringing together technology, medicine, food, culture, and nature, this fascinating book will open your eyes to the wonders of the hidden kingdom all around us.

This technical and well-illustrated guide for archaeologists and conservators aims to 'provide a methodology for the identification of the woody taxa used to manufacture artefacts recovered from archaeological excavations', to provide the anatomical descriptions of the taxa and to present a list of characters of the taxa. The guide is heavily illustrated with photographs, maps, and tables to allow easy identification.