In continuation from the previous three volumes 17, 18, and 19 on High-Tech and Micropropagation this volume presents 29 chapters on the propagation of ornamental plants through modern biotechnological methods. The species covered include "Alstromeria," "Antirrhinum," "Begonia," "Chrysanthemum," "Cornus," "Euphorbia," "Gardenia," "Gladiolus," "Hyacinthus," "Impatiens," "Iris," "Lycoris," "Nematanthus," "Paeonia," "Pelargonium," "Phalaenopsis," "Rhododendron," "Ruscus," "Saintpaulia," "Senecio," "Syringa," orchids, cacti, roses, and Boston ferns. In addition, one chapter is devoted to micropropagation of virus-free ornamentals in the CIS. Throughout the book, detailed protocols as well as a comprehensive review of the literature are provided. Advanced students, teachers, and researchers in the fields of floriculture, horticulture, and plant biotechnology in general, and also those interested in industrial or commercial micropropagation will find a wealth of useful information in the book.

Southern Africa boasts a rich variety of tree species, both indigenous and exotic. Pocket Guide Trees of Southern Africa is an authoritative introduction to the region’s trees, describing and illustrating 132 species.

This publication has its origins in Van Wyk’s A Photographic Guide to Trees of Southern Africa and has been fully revised and updated by well-known authority Braam van Wyk.

Each species account highlights the key identification features, and also touches on the medicinal and commercial uses of the trees.

This compact, easy-to-use guide makes an ideal travelling companion and will help readers to become more knowledgeable about trees.

Presenting the state of the art of tissue culture and in vitro propagation of vegetable and tuber crops, medicinal and aromatic plants, fibre and oilseed crops, and grasses, this book complements the previous two volumes on High-Tech and Micropropagation, which concentrated on special techniques (Vol.17) and trees and bushes of commercial value (Vol.18). The specific plants covered here include asparagus, lettuce, horse radish, cucumber, potato, cassava, sweet potato, artichoke, yams, cardamom, fennel, celery, thyme, leek, mentha, turmeric, lavender, agave, yucca, cotton, jute, sunflower, ryegrass, zoysiagrass, and various species of "Aconitum," "Artemisia," "Camelia," "Centaurium," "Digitalis," "Dioscorea," "Glehnia," "Levisticum," "Parthenium," and "Pinella." The book is of use to advanced students, teachers and research workers in the field of pharmacy, horticulture, plant breeding and plant biotechnology in general, and also to individuals interested in industrial micropropagation.

Biofuels and Rural Poverty makes an original contribution to the current controversial global debate on biofuels, in particular the consequences that large-scale production of transport fuel substitutes can have on rural areas, principally in developing countries but also in some poor rural areas of developed countries.

Three key concerns are examined from a North-South perspective: ecological issues (related to land use and biodiversity), pro-poor policies (related to food and land security, gender and income generation) and equity of benefits within the global value chain. Can biofuels be pro-poor? Can smallholder farmers be equitably integrated in the biofuels global supply chain? Is the biofuels production chain detrimental to biodiversity?

Most other books available on biofuels take a technical approach and are aimed at addressing energy security or climate change issues. This title focuses on the socio-economic impacts on rural people's livelihoods, offering a unique perspective on the potential role of biofuels in reducing rural poverty.

This book continues as volume 6 of a multi-compendium on Edible Medicinal and Non-Medicinal Plants. It covers edible fruits/seeds used fresh, cooked or processed into other by-products, or as vegetables, cereals, spices, stimulant, edible oils and beverages. It covers selected species from the following families: Sapindaceae, Sapotaceae, Schisandraceae, Solanaceae, Thymelaeaceae, Urticaceae, Vitaceae and Winteraceae. This work will be of significant interest to scientists, researchers, medical practitioners, pharmacologists, ethnobotanists, horticulturists, food nutritionists, agriculturists, botanists, conservationists, lecturers, students and the general public. Topics covered include: taxonomy; common/English and vernacular names; origin and distribution; agroecology; edible plant parts and uses; botany; nutritive and pharmacological properties, medicinal uses and research findings; nonedible uses; and selected references.

This volume covers a range of methods used in plant cytogenetics, beginning with basic analysis of chromosomes and visualizing gene locations, to manipulating and dissecting chromosomes, and then focusing on less understood features of chromosomes such as recombination initiation sites and epigenomic marks. The methods described in Plant Cytogenetics: Methods and Protocols build on each other and provide, those new to the field, with a comprehensive platform to support their research endeavours, while also introducing advanced techniques to experienced researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Plant Cytogenetics: Methods and Protocols, is a valuable resource for anyone who is interested in the diverse and wonderfully complex field of cytogenetics.

Dramatically revised and greatly expanded, the second edition of Forensic Botany features triple the amount of images, including a 16-page color insert to aid with macro and micro identification. This edition also features new high profile cases involving the use of botanical evidence, analysis and updating of plant databases, and database sampling. New chapters provide coverage of plant poisons and toxicology, plant bioinformatics, use of plants for death investigation and clandestine grave identification, and the applications of botany to archeology. The book discusses plant biology from a forensic point of view and offers practical guidelines for how to use botanical evidence in a case.

The rapidly growing human population has increased the dependence on fossil fuel based agrochemicals such as fertilizers and pesticides to produce the required agricultural and forestry products. This has exerted a great pressure on the non renewable fossil fuel resources, which cannot last indefinitely. Besides, indiscriminate use ofpesticides for pests (weeds, insects, nematodes, pathogens) control has resulted in serious ecological and environmental problems viz., (A) Increasing incidence of resistance in pest organisms to important pesticides. (B) Shift in pests population, particulary in weeds and insects. In weeds, species that are more closely related to the crops they infest have developed. In insects, scenario is most grim, the predators have been killed and minor insect pests have become major pests and require very heavy doses ofhighly toxic insecticides for their control. (C) Greater environmental pollution and health hazards (a) particularly from contamination of surface and underground drinking water resources and (b) from their inhalation during handling and application. (D) Toxic residues of pesticides pollute the environment and may prove hazardous to even our future generations. (E) Some agricultural commodities may contain minute quantities ofpesticides residues, with long tenn adverse effects on human and livestock health. Therefore, serious ecological questions about the reliance on pesticides for pests control has been raised. The use of fertilizers, besides causing environmental problems has also impoverished the soil health and decreased the beneficial soil fauna. For example, in some major crop rotations viz."

This volume provides a comprehensive collection of protocols that can be used to study plant chromatin structure and composition. Chapters divided into three sections detail the profiling of chromatin features in relation to epigenetic regulation, investigate the interaction between chromatin modifications and gene regulation, and explore the 3D spatial organization of the chromatin inside the nucleus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Chromatin Dynamics: Methods and Protocols aims to ensure successful results in the further study of this vital field.

As we approach the bicentennial of the Lewis and Clark Expedition in 2004, attention will inevitably turn to the nineteenth-century explorers who risked life and limb to interpret the natural history of the American West. Beginning with Meriwether Lewis and his discovery of the bitterroot, the goal of most explorers was not merely to find an adequate route to the Pacific, but also to comment on the state of the region's ecology and its suitability for agriculture, and, of course, to collect plant specimens. In this book, Williams follows the trail of over a dozen explorers who "botanized" the Rocky Mountains, and who, by the end of the nineteenth century, became increasingly convinced that the flora of the American West was distinctive. The sheer wonder of discover, which is not lost on Williams or his subjects, was best captured by botanist Edwin James in 1820 as he emerged above timberline in Colorado to come upon "a region of astonishing beauty."

In recent years there has been an unprecedented expansion of knowledge about anthocyanins pigments. Indeed, the molecular genetic control of anthocyanins biosynthesis is now one of the best understood of all secondary metabolic pathways. There have also been substantial improvements in analytical technology that have led to the discovery of novel anthocyanin compounds. Armed with this knowledge and the tools for genetic engineering, plant breeders are now introducing vibrant new colors into horticultural crops. The food industry has also benefited from the resurgence of interest in anthocyanins. A greater understanding of the chemistry of these pigments has led to improved methods for stabilizing the color of anthocyanins extracts, so that they are more useful as food colorings. Methods for the bulk production of anthocyanins from cell cultures have been optimized for this purpose. Possible benefits to human health from the ingestion of anthocyanin-rich foods have also been a major feature of the recent scientific literature. Anthocyanins are remarkably potent antioxidants, and their ingestion has been postulated to stave off the effects of oxidative stress. These pigments, especially in conjunction with other flavonoids, have been associated with reductions in the incidence and severity of many other non-infectious diseases, including diabetes, cardiovascular disease and certain cancers. An industry is developing around anthocyanins as nutritional supplements. Finally, there has been significant progress in our understanding of the benefits of anthocyanins to plants themselves. Originally considered an extravagance without a purpose, anthocyanins are now implicated in multifarious vital functions. These include the attraction of pollinators and frugivores, aposematic defense from herbivores, and protection from environmental stressors such as strong light, UVB, drought, and free radical attacks. Anthocyanins are evidently highly versatile, and enormously useful to plants. This book covers all aspects of the biosynthesis and function of anthocyanins (and related compounds such as proanthocyanidins) in plants, and their applications in agriculture, food products, and human health. Featured areas include their relevance to: * Plant stress * Flower and fruit color * Human health * Wine quality and health attributes * Food colorants and ingredients * Cell culture production systems * The pastoral sector

Plant Cell and Tissue Culture continues the high standards of Humana's Methods in Molecular Biology series. Its step-by-step approach (a hallmark of the series) is applied to a wide range of basic laboratory techniques and culture conditions appropriate to plant cells. Because of the diversity of cell types, species, and culture methods, much of this volume is devoted to the culture of particular cell types and to the regeneration of these cells into whole plants. Special attention is also given to the genetic modification of plants, as well as to the economic significance of plant products. Chapters cover a wide range of topics and techniques, including:* tissue culture media and selection * cryopreservation * callus culture techniques * organ culture * embryogenesis * batch culture * large-scale culture * hormonal control * fertilization techniques * gene transfer * cell immobilization * production systems * cell product purification * DNA expression * electrofusion of plant cells * mutant selection * mutagenesis techniques * automation * transfer of nuclei * protoplast culture * media analysis * micropropagation. A detailed appendix lists the formulas for the most commonly employed plant cell media. Comprehensive, easy to follow, and a pleasure to use, Pollard and Walker's Plant Cell and Tissue Culture is an essential tool for everyone--at all levels of proficiency and experience--involved in plant culture.

Mulberry (Morus spp.) is an important horticultural plant in the sericulture industry. It belongs to the family Moraceae. The leaf of mulberry is used to feed the silkworm Bombyx mori L. It is also used as a fodder. Due to its economic and agricultural importance, mulberry is cultivated in many parts of the world. An estimated 60% of the total cost of silk cocoon production is for production and maintenance of mulberry plants. Therefore, much attention is needed to improve the quality and quantity of mulberry leaves. It is vital to increase the production of superior quality mulberry leaves with high nutritive value for the sericulture industry. Although a lot of research is going on in mulberry, very little effort has been made to compile the results of this research in a single book. This book provides an update of recent research works going on in this plant. It describes the taxonomy, conservation of germplasm, genetic diversity of various mulberry species, application of breeding techniques to improve the quality of mulberry, in vitro conservation, application of tissue culture techniques to improve mulberry species, production of haploids and triploids in mulberry and improvement of abiotic stress adaptive traits in mulberry with relevance to adaptiveness to global warming.

Doing English Language provides a concise, lively and accessible introduction to the field of English Language studies for readers who are interested in taking courses at university level. This book addresses the fundamental questions about studying English Language, including: How is English Language studied and researched? Which subject areas does English Language draw on? How are different topics approached? How is the study of English Language relevant to real world contexts? What careers can English Language lead to? Written by an experienced teacher, researcher, and examiner, Doing English Language is both an essential guide for students at pre-university stage and a course companion for undergraduates choosing options within a degree programme.

Virus Variability and Impact on Epidemiology and Control of Diseases E. Kurstak and A. Hossain I. INTRODUCTION An important number of virus infections and their epidemic developments demonstrate that ineffec tiveness of prevention measures is often due to the mutation rate and variability of viruses (Kurstak et al., 1984, 1987). The new human immunodeficiency retroviruses and old influenza viruses are only one among several examples of virus variation that prevent, or make very difficult. the production of reliable vaccines. It could be stated that the most important factor limiting the effectiveness of vaccines against virus infections is apparently virus variation. Not much is, how ever, known about the factors influencing and responsible for the dramatically diverse patterns of virus variability. II. MUTATION RATE AND VARIABILITY OF HUMAN AND ANIMAL VIRUSES Mutation is undoubtedly the primary source of variation, and several reports in the literature suggest that extreme variability of some viruses may be a consequence of an unusually high mutation rate (Holland et al., 1982; Domingo et al., 1985; Smith and Inglis, 1987). The mutation rate of a virus is defined as the probability that during a single replication of the virus genome a particular nucleotide position is altered through substitution, deletion, insertion. or recombination. Different techniques have been utilized to measure virus mutation rates, and these have been noted in the extent of application to different viruses."

Hazardous and Trace Materials in Soil and Plants: Sources, Effects and Management explores the latest advancements in reducing, avoiding and eliminating soil contaminants that challenge the health and safety of agricultural plants. With a focus on minimizing the production of those hazardous substances, controlling their distribution and ensuring safe utilization, the book explores each contributing area and provides insights toward improved, sustainable and secure production. This is an excellent reference resource on both current research and future directions from laboratory research to field applications. The combined impacts of climate change and industrialization have led to increased and diversified threats to the health of the soil in which our food crops are grown, as well as in the plants themselves. This dual-hazard scenario is increasingly recognized as a threat to not just the environment, but to global food security as agricultural soils contaminated with pollutants alter plant metabolism, thus resulting in reduced crop quality and production quantity.

Viral Genomic RNA as a Single Translation Unit 100 Initiation Codon Choice and Translation in Plants 100 Conventional Scanning and Initiation 100 Non-AUG Initiation Codons in Plant Virus RNAs 101 Role of5'- and 3'- Noncoding Regions in Initiation of Translation 101 Access to Internal Genes 103 Divided versus Monopartite Genomes 104 Transcriptional Control: Subgenomic RNAs 104 Non-Orthodox Mechanisms of Initiation 107 Leaky Scanning 107 Internal Ribosome Entry Sites (IRES Elements) 107 Non-Orthodox Mechanisms of Elongation and Termination 108 Readthrough of Leaky Stop Codons 108 Ribosomal Frameshifiting 109 Proteolytic Processing 110 Chymotrypsin-like Serine or Cysteine Proteinases 112 Papain-like Cysteine Proteinases 112 Conclusions 113 References 114 5. Molecular Basis of Genetic Variability in RNA Viruses 121-141 Jozef J. Bujarski Introduction 121 Genetic Mechanisms of Variability 121 Mutation 121 Point Mutations 122 Insertions and Deletions 123 Frameshift Mutations 123 Mutant Stability 124 Effects of Mutations on Host-Virus Interactions 124 RNA Recombination 125 Natural Sequence Rearrangement 126 Recombination between Viral and Host RNAs 126 Role of RNA Structure 127 Role of Replicase Proteins 130 Pseudorecombination 131 Defective Interfering RNAs 132 Conclusions 134 Acknowledgements 136 References 136 viii 6. Genetic Variability and Evolution 143-159 F. Garcia-Arenal, A. Fraile, and J. M. Malpica Introduction 143 Variability under Experimental Conditions 143 Variability under Natural Conditions 145 Factors Determining Genetic Structure of Virus Populations 149 Founder Effects 149 Selection 150 Complementation 152 Conclusions 153 Acknowledgements 155 References 155 7. Molecular Basis of Virus Transport in Plants 161-182 Scott M.

Nectar is the most important reward offered by plants to pollinating animals. This book is a modern and interdisciplinary text on nectar and nectaries, prompted by the expansion of knowledge, especially in the more ecological and now molecular fields, and the strong recent interest in pollination biology. The topics covered vary widely: they include historical aspects, the structure and ultrastructure of nectaries and relationships to plant systematics, the dynamics of nectar secretion, nectar chemistry and the molecular biology of defence proteins, adaptations to insect and vertebrate nectar consumers and consequences for pollination ecology, and broad-scale studies of nectar resources at the community level.

The first book to chronicle how innovation in laboratory designs for botanical research energized the emergence of physiological plant ecology as a vibrant subdiscipline   Laboratory innovation since the mid-twentieth century has powered advances in the study of plant adaptation, evolution, and ecosystem function. The phytotron, an integrated complex of controlled-environment greenhouse and laboratory spaces, invented by Frits W. Went in the 1950s, set off a worldwide laboratory movement and transformed the plant sciences. Sharon Kingsland explores this revolution through a comparative study of work in the United States, France, Australia, Israel, the USSR, and Hungary.   These advances in botanical research energized physiological plant ecology. Case studies explore the development of phytotron spinoffs such as mobile laboratories, rhizotrons, and ecotrons. Scientific problems include the significance of plant emissions of volatile organic compounds, symbiosis between plants and soil fungi, and the discovery of new pathways for photosynthesis as an adaptation to hot, dry climates. The advancement of knowledge through synthesis is a running theme: linking disciplines, combining laboratory and field research, and moving across ecological scales from leaf to ecosystem. The book also charts the history of modern scientific responses to the emerging crisis of food insecurity in the era of global warming.