Genome Mapping and Molecular Breeding in Plants presents the current status of the elucidation and improvement of plant genomes of economic interest. The focus is on genetic and physical mapping, positioning, cloning, monitoring of desirable genes by molecular breeding and the most recent advances in genomics. The series comprises seven volumes: Cereals and Millets; Oilseeds; Pulses, Sugar and Tuber Crops; Fruits and Nuts; Vegetables; Technical Crops; and Forest Trees.

Technical Crops includes plants of great agricultural importance. One chapter is devoted to cotton, the most important fiber crop on which significant progress in molecular genetic research has been made. Reviews on oil palm, coffee, tea, cocoa and rubber describe traditional breeding and preliminary molecular results. Chapters on forage crops, ornamentals, and medicinal and aromatic plants each cover a large number of crops and may serve as road maps for further molecular research.

The present book provides a comprehensive overview of our current knowledge on plastid biogenesis, plastid-nuclear communication, and the regulation of plastid gene expression at all levels. It also assesses the state-of-the-art in key technologies, such as proteomics and chloroplast transformation. Written by recognized experts in the field, the book further covers crucial post-translational processes in plastid biogenesis and function, including protein processing.

The adv antages of those systems are counterbalanced by some important dis- vantages. For one, in heterotrophic and mixotrophic systems high concentrations of organic ingredients are required in the nutrient medium (particularly sugar at 2% or more), associated with a high risk of microbial contamination. How, and to which extent this can be avoided will be dealt with in Chapter 3. Other disadvantages are the difficulties and limitations of extrapolating results based on tissue or cell c- tures, to interpreting phenomena occurring in an intact plant during its development. It has always to be kept in mind that tissue cultures are only model systems, with all positive and negative characteristics inherent of such experimental setups. To be realistic, a direct duplication of in situ conditions in tissue culture systems is still not possible even today in the 21st century, and probably never will be. The organization of the genetic system and of basic cell structures is, however, essentially the same, and therefore tissue cultures of higher plants should be better suited as model s- tems than, e.g., cultures of algae, often employed as model systems in physiological or biochemical investigations. The domain cell and tissue culture is rather broad, and necessarily unspecif ic. In terms of practical aspects, basically five areas can be distinguished (see Figs. 1.1, 1.2 ), which here shall be briefly surveyed before being discussed later at length.

The importance of haploids is well known to geneticists and plant breeders. The discovery of anther-derived haploid Datura plants in 1964 initiated great excitement in the plant breeding and genetics communities as it offered shortcuts in producing highly desirable homozygous plants. Unfortunately, the expected revolution was slow to materialise due to problems in extending methods to other species, including genotypic dependence, recalcitrance, slow development of tissue culture technologies and a lack of knowledge of the underlying processes. Recent years have witnessed great strides in the research and application of haploids in higher plants. After a lull in activities, drivers for the resurgence have been: (1) development of effective tissue culture protocols, (2) identification of genes c- trolling embryogenesis, and (3) large scale and wide spread commercial up-take in plant breeding and plant biotechnology arenas. The first major international symposium on "Haploids in Higher Plants" took place in Guelph, Canada in 1974. At that time there was much excitement about the potential benefits, but in his opening address Sir Ralph Riley offered the following words of caution: "I believe that it is quite likely that haploid research will contr- ute cultivars to agriculture in several crops in the future. However, the more extreme claims of the enthusiasts for haploid breeding must be treated with proper caution. Plant breeding is subject from time to time to sweeping claims from ent- siastic proponents of new procedures.

This manual is intended for the practising chemist who has to do a job in analysing plant material. Therefore, the present manual only contains ready-to-hand procedures without any comment. The procedures described are only for inorganic components, which frequently occur in the plant. Most procedures are designed to give a total content value of the element under consideration, regardless of the chemical structure in which it occurs in the plant.

We have chosen for a design in which all digestion procedures are described in one chapter, all extraction procedures in one chapter and all determination procedures in one chapter. As a consequence, one has to choose a suitable digestion method in combination with the intended determination technique; this has been indicated within each individual determination procedure.

For determination of the elements, mainly spectrometric techniques are used here. Depending on the kind of element and the expected concentration level, the following methods are applied: flame atomic emission spectrometry (flame AES), flame atomic absorption spectrometry (flame AAS), inductively coupled plasma optical emission spectrometry (ICP-OES), electrothermal atomisation (graphite furnace) atomic absorption spectrometry (ETA-AAS), inductively coupled plasma mass spectrometry (ICP-MS), spectrophotometry and segmented flow analysis (SFA). Besides, potentiometry (ion selective electrodes (ISE)) and coulometry will be encountered. In many cases, more than one method is described to determine a component. This provides a reference, as well as an alternative in case of instrumental or analytical problems.

Palynology is important in basic as well as in manifold applied sciences, as e.g. biology, medicine, forensics, earth history, climatology and food production. This volume is the first fully illustrated handbook of palynological principles and glossary terms, exclusively using LM and EM micrographs of superior quality. A comprehensive General Chapter on pollen morphology, anatomy, pollen development etc. based on the present knowledge in palynology introduces the reader in the world of pollen. The glossary part comprises more than 300 widely used terms illustrated with over 1.000 high quality light and/or electron microscopic pictures to show the character range of a term. Terms are grouped by feature, e.g. ornamentation, where each term is illustrated on a separate page, definition and original citation included and where necessary, provided with a comprehensive explanatory comment. The term's use in LM, SEM or TEM and its assignment to anatomical, morphological and/or functional pollen features is indicated by icons and colour coding, respectively. This handbook is not only a valuable source for students and researchers but also for all persons interested in pollen and its aesthetic beauty.

During the past decade, there has been tremendous progress in maize biotechnology. This volume provides an overview of our current knowledge of maize molecular genetics, how it is being used to improve the crop, and future possibilities for crop enhancement. Several chapters deal with genetically engineered traits that are currently, or soon will be, in commercial production. Technical approaches for introducing novel genes into the maize genome, the regeneration of plants from transformed cells, and the creation of transgenic lines for field production are covered. Further, the authors describe how molecular genetic techniques are being used to identify genes and characterize their function, and how these procedures are utilized to develop elite maize germplasm. Moreover, molecular biology and physiological studies of corn as a basis for the improvement of its nutritional and food-making properties are included. Finally, the growing use of corn as biomass for energy production is discussed.

Molecular Mechanisms of Plant and Microbe Coexistence presents studies on the complex and manifold interactions of plants and microbes at the population, genomics and proteomics level. The role of soil microbial diversity in enhancing plant health and plant microbe beneficial symbioses is discussed. Microbial communities are shown in the light of evolution. Main topics include genome coexistence and the functional genomics and proteomics of plant-associated microbes, which could form the basis for new environmentally benign strategies to combat infectious plant diseases and regulate plant growth. Further chapters focus on the role of signaling during the different stages of plant microbe coexistence, in symbiotic or pathogenic relationships, in quorum sensing and plant viral infections. Methods for studying the interactions in the root zone complement the book, which will certainly be of relevance in the practical application to agriculture, food security and for maintaining the balance of our ecosystems.

The main theme of the book is sustainable disease management in a European context. Some of the questions addressed are: How does society benefit from plant pathology research? How can new molecular approaches solve relevant problems in disease management? What other fields can we exploit in plant pathology research? What challenges are associated with free trade across the new borders? How can we contribute to solving problems of developing countries? How does plant pathology contribute to food quality and safety? How does globalization/internationalization affect teaching and extension in plant pathology?

Christel Ross investigates whether evolutionary changes by plant breeding are an important factor for the invasion success of Mahonia aquifolium in Germany. Her findings show that invasive populations differ from native populations in quantitative-genetic traits and molecular markers, whereas their genetic diversity is similar. She postulates that these evolutionary changes are rather a result of plant breeding, which includes interspecific hybridisation, than the result of a genetic bottleneck or the releases from specialist herbivores.

This title includes a number of Open Access chapters. In horticulture, agriculture, and food science, plants' reproductive physiology is an important topic relating to fruits and vegetables, the main consumable parts of plants. All aspects of plant physiology, including plants' reproductive systems, are important to the production of food, fibers, medicine, cosmetics, and even fuels. This volume presents many new studies on plants' reproductive systems, including new research on sperm cells in plant reproduction; the effect of herbivory on plant reproduction; disturbances to functional diversity; plant genes, hormones, DNA; and much more.

Molecular farming is a biotechnological approach that includes the genetic adjustment of agricultural products to create proteins and chemicals for profitable and pharmaceutical purposes. Plant molecular farming describes the manufacture of recombinant proteins and other biologically active product in plants. This approach depends on a genetic transformation of plants that can be accomplished by the methods of stable gene transfer, such as gene transfer to nuclei and chloroplasts, and unstable transfer methods like viral vectors. The requirement for recombinant proteins in terms of quality, quantity, and diversity is increasing exponentially This demand is traditionally met by recombinant protein construction technologies and the engineering of orthodox expression systems based on bacteria or mammalian cell cultures. However, majority of developing countries cannot afford the high costs of medicine derived from such existing methods. Hence, we need to produce not only the new drugs but also the cheaper versions of those already present in the market. Plant molecular farming is considered as a cost-effective technology that has grown and advanced tremendously over the past two decades. This book summarizes the advances and challenges of plant molecular farming for all those who are working on or have an interest in this rapidly emerging area of research.

The series is designed to meet the needs of students and lecturers of the National Certificate Vocational. To facilitate students' learning, the following features are used in the series: Content is written in easy-to-understand language, key terms are carefully explained, using everyday English, case studies show how to apply the theory in the work environment, the study skills sections help students make the most of their learning in class and prepare for the exams, there are many practice activities and questions with model answers at the back of the title, checklists assist students to make sure that they have covered all the skills and content in each chapter, and summaries at the end of each chapter are useful for exam revision. Lecturers using the series can teach with confidence because content is comprehensive, up-to-date, and meets all the curriculum requirements for the subject, outcomes and assessment standards are clearly identified, and assessment tasks and activities are aligned to the outcomes and assessment standards. Prescribing lecturers have access to comprehensive lecturer support material on CD including model answers to assessments in the textbook, additional assessments with model answers, rubrics for assessments, and general reference material on teaching outcomes-based education. The series is available for all programmes, all fundamental and compulsory subjects, and all elective and optional subjects.

This successful text examines the ecological processes that determine the size and structure of a population, and demonstrates that there are many fundamental principles that apply to populations of both animals and plants. The authors have sought to involve the text in areas where particular progress has been made and consolidated. The third edition therefore addresses the problem of the detection of chaos, buried sea-banks and herbivorey implants and looks more closely at the role of spatial scale in two-species interactions. It also introduces a major new section on the concept of the meta-population. The book is designed to be readily understood by undergraduates with little or no previous knowledge of the subject, and also to stimulate wider debate and discussion.

Healthy seeds and propagules are the basic requirement for producing good grains, fruits and vegetables needed for human survival and perpetuation. Dispersal of microbial plant pathogens via seeds and propagules has assumed more importance than other modes of dispersal, as infected seeds and propagules have the potential to become the primary sources of carrying pathogen inoculum for subsequent crops. Several diseases transmitted through seeds and propagules have been shown to have the potential to damage economies as a result of huge quantitative and qualitative losses in numerous crops. Hence, it is essential to rapidly detect, identify and differentiate the microbial plant pathogens present in seeds and propagules precisely and reliably, using sensitive techniques. Microbial Plant Pathogens: Detection and Management in Seeds and Propagules provides a comprehensive resource on seed-borne and propagule-borne pathogens. Information on the biology of microbial pathogens, including genetic diversity, infection process and survival mechanisms of pathogens and epidemiology of diseases caused by them, are discussed critically and in detail to highlight weak links in the life cycles of the pathogens. Development of effective disease management systems, based on the principles of exclusion and eradication of pathogens and immunization of crop plants to enhance the levels of resistance of cultivars to diseases, has been effective to keep the pathogens at bay. The need for production of disease-free seeds/propagules has been emphasized to prevent the carryover of the inoculum to the next crop or introduction of the pathogens to other locations. Effectiveness of adopting simple cultural practices and development of cultivars resistant to diseases through traditional breeding methods or biotechnological approach have resulted in reducing the pathogen inoculum and disease incidence. Although application of different chemicals may reduce the disease incidence effectively, biological management of crop diseases, employing potential biological control agents have to be preferred to preserve the agroecosystems. Greater efforts have to be made to integrate compatible strategies to enhance the effectiveness of diseases management systems. Protocols appended at the end of relevant chapters form a unique feature of this book to enable the researchers to fine-tune their projects. This 2 volume set provides comprehensive and updated information about the economically-important groups of microbial plant pathogens carried by seed and propagules. Graduate students, researchers and teachers of plant pathology, plant protection, microbiology, plant breeding and genetics, agriculture and horticulture, as well as certification and quarantine personnel will find the information presented in this book useful.

In a new series in plant conservation sponsored by the World Wide Fund for Nature (WWF), this book is a practical guide to a subject of increasing concern to the conservation biologist. As with each of the books in the series, this book aims to give an authoritative guide to the subject in a clear, jargon-free style. It should therefore not only be of interest to the academic biologist interested in the phenomenon of invasion, but also to government and non-governmental personnel involved in the decision-making process needed as a result of the problems encountered with invasive species. Case-studies highlight specific problems from a wide range of countries, including North America, South and Central America, Africa, Australasia, South and South East Asia, Europe, the Pacific and Atlantic Islands and Indonesia. The book contains an appendix with a list of invasive species and their countries of origin. A bibliography contains reference to all cited articles and books. This book should be of interest to conservation biologists, economic botanists, ethnobotanists, plant ecologists, agriculturalists, those interested in development problems, geographers, environmental scientists, ecotourists and "green" lay people.

This book presents the life science experiments in a space microgravity environment conducted on board the SJ-10 recoverable satellite, which was launched on April 6th 2016 and recovered on April 18th 2016. It covers 10 scientific projects in radiation biology, gravitational biology and biotechnology that were selected from ~100 proposals from various institutions in China and around the world. Primarily exploring the rhythm of life in a space microgravity environment, all of the experiments - conducted on nine payloads of the SJ-10 satellite - have never been previously conducted in the respective fields. In addition, the book provides extensive information on the mission's execution, data collection, and scientific outcomes.

Plant hormones play a crucial role in controlling the way in which plants growand develop. Whilemetabolism providesthepowerand buildingblocks for plant life, it is the hormones that regulate the speed of growth of the individual parts and integrate these parts to produce the form that we recognize as a plant. In addition, theyplayacontrolling role inthe processes of reproduction. This book is a description ofthese natural chemicals: how they are synthesizedand metabolized; howthey work; whatwe knowoftheir molecular biology; how we measure them; and a description ofsome ofthe roles they play in regulating plant growth and development. Emphasis has also been placed on the new findings on plant hormones deriving from the expanding use ofmolecular biology as a tool to understand these fascinating regulatory molecules. Even at the present time, when the role of genes in regulating all aspects of growth and development is considered of prime importance, it is still clear that the path of development is nonetheless very much under hormonal control, either via changes in hormone levels in response to changes in gene transcription, or with the hormones themselves as regulators ofgene transcription. This is not a conference proceedings, but a selected collection ofnewly written, integrated, illustrated reviews describing our knowledge of plant hormones, and the experimental work that is the foundation of this knowledge.

This textbook contains the material for a course in the major principles of modelling crop growth processes. There is much more to crop growth than what is discussed in this textbook, but it provides a sound basis for further work and study in this field. Chapter by chapter the book leads the reader to different modelled aspects of crop growth, and at the end, the reader will have a good understanding of the Wageningen simulation model SUCROS for the potential production situation. By then, it will be much easier to find one's way through descriptions and listings of other models. Throughout the text, the study of the different topics is facilitated by exercises that support the course in a hands-on computer practical exercise. A very simple crop growth model, almost entirely based on radiation interception, is given first. This skeleton model is then expanded by submodels for respiration, carbon assimilation, plant development, and a more detailed model for radiation interception and reflection. Modelling of transpiration and the leaf energy balance is given by way of introduction. There are many listings of the submodels, written in the simulation language FST (FORTRAN Simulation Translator), as well as of SUCROS itself, together with plentiful comments. Some supporting theory is provided in the form of Appendices. The book is meant for students and scientists who would like to acquire a working knowledge of the technique of crop growth modelling.

This book serves as the first comprehensive compilation describing the breeding strategies and genetics and genomics of the coconut palm. It describes gene evolution of economically important traits such as oil biosynthesis, aroma and fragrance, disease-resistant genes and small RNAs-mediated gene regulation of coconut. Application of "omics" approaches in palms and the prospects of genome editing technologies in coconut are also discussed. The author list includes pioneers and experts in the field of coconut genomics. The book appeals to postgraduate students, researchers and industry players in the field of plantation crops in general and coconut in particular.

Flowering and fruiting are key processes in the biology of higher plants, ensuring the transfer of genetic material from one generation to the next. In addition, as almost all of the world's agricultural and horticultural industries depend on the production of flowers, fruits and seeds, the study of the reproductive biology of cultivated plants is of fundamental importance to humankind. Surprisingly, therefore, this topic has received relatively little attention from environmental physiologists compared with studies on the growth and development of vegetative structures. This book, based on a meeting held by the Environmental Physiology Group of the Society of Experimental Biology, sets out to correct this deficiency. The topic is given a broad and comprehensive treatment, with chapters covering the onset of flowering through to the development and growth of fruits and seeds, and finally to ecological and evolutionary aspects of fruiting. This volume will therefore serve as a useful introduction to the various aspects of flowering and fruiting and will also provide a thorough general overview of the subject for students and researchers alike.

This supplement, containing six chapters, is the first in a series of important works designed to be integrated into the text of the Plant Tissue Culture Manual to maintain it as a valuable source of laboratory methodology

Grasses are a principal source of food for mankind and play an important role in stabilizing the land surface of much of the globe. Understanding seed dormancy in the Gramineae is therefore of considerable significance to world agriculture and global ecology. This book provides a comprehensive review of the occurrence and explanation of seed dormancy in grasses. Experimental evidence is considered in depth for a single species, the wild oat (Avena fatua), probably the most widely studied species for understanding seed dormancy in the plant kingdom. The evidence for this species is compared with other examples among the Gramineae to reach some general conclusions about the nature of seed dormancy in grasses. Essential reading for all those who need to understand the mechanisms of seed dormancy, this book will be a valuable text for advanced students and professionals in plant physiology, crop science, plant breeding and agronomy.

This book presents edited and revised papers from the seventh International Workshop on Seeds, held in Salamanca, Spain, in May 2002. The key topics addressed include seed development, germination and dormancy, as well as desiccation, seed ecology and seed biotechnology.

The germination of seeds is a magical event, in which a pinch of dust-like material may give rise to all the power and the beauty of the growing plant. The mechanisms of seed dormancy, of the breaking of seed dormancy and of germination itself continue to remain shrouded in mystery, despite the best efforts of plant scientists. Perhaps we are getting there, but very slowly. This book considers germination and dormancy from the point of view of plant physiology. Plant physiologists attempt to understand the relation ship between plant form and function and to explain, in physical and chemical terms, plant growth and development. The place of germination and dormancy in plant ecophysiology is taken into account with attempts to understand the seed in its .environment, whether the environment be natural, semi-natural or wholly artificial. In due course plant scientists hope to develop a precise understanding of germination and dormancy in cellular and molecular terms, and therefore there is some biochemistry in this book. Biochemists who wish to learn something about seeds should find this book useful."