Genetic engineering and biotechnology along with conventional breeding have played an important role in developing superior cultivars by transferring economically important traits from distant, wild and even unrelated species to the cultivated varieties which otherwise could not have been possible with conventional breeding. There is a vast amount of literature pertaining to the genetic improvement of crops over last few decades. However, the wonderful results achieved by crop scientists in food legumes' research and development over the years are scattered in different journals of the World. The two volumes in the series 'Alien Gene Transfer in Crop Plants' address this issue and offer a comprehensive reference on the developments made in major food crops of the world. These volumes aim at bringing the contributions from globally renowned scientists at one platform in a reader-friendly manner. The second volume entitled, "Alien Gene Transfer in Crop Plants: Achievements and Impact" will deal more with the practical aspects. This volume will cover achievements of alien gene transfer in major food crops of the world and their impact on development of newer genetic variability and additional avenues for selection; development of superior cultivars for increased yield, resistance to biotic and abiotic stresses, improved nutritional and industrial quality; innovation of new techniques and positive as well as negative environmental implications. This volume has been divided into four groups with an aim to cover all major cereals, pulses, oilseeds and other crops (vegetable and horticultural crops) which are of economic importance.

This volume examines the interrelated fields of food security, energy security and sustainable agriculture as the key to a stable global agricultural platform and is arranged in six parts. The first part is focused on policy considerations relating to food and energy security and sustainable agriculture. The authors from this part include Former Under Secretary of Agriculture Gale Buchanan, Former Under Secretary of Energy Raymond Orbach (Chapter 1), Stephen Hughes, Bryan Moser and William Gibbons (Chapter 2) and Thomas Redick (Chapter 3). Part II addresses soil and water, which are two of the key components in secure and sustainable food production. Authors from this part are Jerry Hatfield (Chapter 4) and Mahbub Alam, Sharon Megdal et al. (Chapter 5). The third part covers sustainable and secure food production specifically addressing genetically modified traits in Chapter 6 (James McWilliams) and omega-3 fatty acids in Chapter 7 (Jay Whelan et al.). Agronomic implications relative to food security and sustainable agriculture are described in Part IV. Authors include Ravi Sripada, Pradip Das et al. (Chapter 8), Duska Stojsin, Kevin Matson and Richard Leitz (Chapter 9) and S.H. Lee, David Clay and Sharon Clay (Chapter 10). International sustainable agriculture and food security is addressed in Part V with authors Jeff Vitale and John Greenplate (Chapter 11), Julie Borlaug et al. (Chapter 12) and Sylvester Oikeh et al. (Chapter 13). The final part covers the use of chemicals in sustainable agriculture and food/energy security with Leonard Gianessi and Ashley Williams communicating the role of herbicides and Harold Reetz emphasizing the importance of fertilizers both in maximizing crop yields to maintain a sustainable secure source for food production.

The field of plant breeding has grown rapidly in the last decade with breakthrough research in genetics and genomics, inbred development, population improvement, hybrids, clones, self-pollinated crops, polyploidy, transgenic breeding and more. This book discusses the latest developments in all these areas but explores the next generation of needs and discoveries including omics beyond genomics, cultivar seeds and intellectual and property rights. This book is a leading-edge publication of the latest results and forecasts important areas of future needs and applications.

This book provides detailed and comprehensive information on oxidative damage caused by stresses in plants with especial reference to the metabolism of reactive oxygen species (ROS). In plants, as in all aerobic organisms, ROS are common by-products formed by the inevitable leakage of electrons onto O2 from the electron transport activities located in chloroplasts, mitochondria, peroxisomes and in plasma membranes or as a consequence of various metabolic pathways confined in different cellular loci. Environmental stresses such as heat, cold, drought, salinity, heavy-metal toxicity, ozone and ultraviolet radiation as well as pathogens/contagion attack lead to enhanced generation of ROS in plants due to disruption of cellular homeostasis. ROS play a dual role in plants; at low concentrations they act as signaling molecules that facilitate several responses in plant cells, including those promoted by biotic and abiotic agents. In divergence, at high levels they cause damage to cellular constituents triggering oxidative stress. In either case, small antioxidant molecules and enzymes modulate the action of these ambivalent species.

Plant Breeding Reviews presents state-of-the-art reviews on plant genetics and the breeding of all types of crops by both traditional means and molecular methods. Many of the crops widely grown today stem from a very narrow genetic base; understanding and preserving crop genetic resources is vital to the security of food systems worldwide. The emphasis of the series is on methodology, a fundamental understanding of crop genetics, and applications to major crops. It is a serial title that appears in the form of one or two volumes per year.

The importance of haploids is well known to geneticists and plant breeders. The discovery of anther-derived haploid Datura plants in 1964 initiated great excitement in the plant breeding and genetics communities as it offered shortcuts in producing highly desirable homozygous plants. Unfortunately, the expected revolution was slow to materialise due to problems in extending methods to other species, including genotypic dependence, recalcitrance, slow development of tissue culture technologies and a lack of knowledge of the underlying processes. Recent years have witnessed great strides in the research and application of haploids in higher plants. After a lull in activities, drivers for the resurgence have been: (1) development of effective tissue culture protocols, (2) identification of genes c- trolling embryogenesis, and (3) large scale and wide spread commercial up-take in plant breeding and plant biotechnology arenas. The first major international symposium on "Haploids in Higher Plants" took place in Guelph, Canada in 1974. At that time there was much excitement about the potential benefits, but in his opening address Sir Ralph Riley offered the following words of caution: "I believe that it is quite likely that haploid research will contr- ute cultivars to agriculture in several crops in the future. However, the more extreme claims of the enthusiasts for haploid breeding must be treated with proper caution. Plant breeding is subject from time to time to sweeping claims from ent- siastic proponents of new procedures.

The book mainly describes the QTL mappings and efficacy analyses that are associated with wheat productivity, quality, physiology and various stress resistances and provides summaries of results from studies conducted both at home and abroad. It presents comparable data and analyses, helping readers to arrive at a more comprehensive understanding of the latest development in this field. The book provides a wealth of novel information, broad range of applications and in-depth findings on crop genetics and molecular breeding, making it valuable not only for plant breeders but also for academic faculties, senior researchers and advanced graduate students who are involved in plant breeding and genetics. Dr. Jichun Tian is a professor at the Department of Agronomy, Shandong Agricultural University, Tai'an, China.

This book provides a comprehensive description of phosphate solubilizing microorganisms and highlights methods for the use of microphos in different crop production systems. The focus is on understanding both the basic and applied aspects of phosphate solubilizing microorganisms and how phosphorus-deficient soils can be transformed into phosphorus-rich ones by applying phosphate solubilizing microorganisms. The interaction of rhizosphere phosphate solubilizing microorganisms and environmental variables, as well as their importance in the production of crops such as legumes, cereals, vegetables etc. are discussed and considered. The use of cold-tolerant phosphate solubilizing microorganisms to enhance crop productivity in mountainous regions is examined, as are the ecological diversity and biotechnological implications of phosphate solubilizing microorganisms. Lastly, the role of phosphate solubilizing microorganisms in aerobic rice cultivation is highlighted. This volume offers a broad overview of plant disease management using phosphate solubilizing microbes and presents strategies for the management of cultivated crops. It will therefore be of special interest to both academics and professionals working in the fields of microbiology, soil microbiology, biotechnology and agronomy, as well as the plant protection sciences. This timely reference book provides an essential and comprehensive source of material, as it includes recent findings on phosphate solubilizing microorganisms and their role in crop production.

This Soil Biology volume examines our current understanding of the mechanisms involved in the beneficial effects transferred to plants by endophytes such as rhizobial, actinorhizal, arbuscular mycorrhizal symbionts and yeasts. Topics presented include how symbiosis starts on the molecular level; chemical signaling in mycorrhizal symbiosis; genomic and functional diversity of endophytes; nitrogen fixation; nutrient uptake and cycling; as well as plant protection against various stress conditions. Further, the use of beneficial microorganisms as biopesticides is discussed, particularly the application of Plant Growth Promoter Rhizobacteria (PGPR) in agriculture with the aim to increase yields.

The improvement of crop species has been a basic pursuit since cultivation began thousands of years ago. To feed an ever increasing world population will require a great increase in food production. Wheat, corn, rice, potato and few others are expected to lead as the most important crops in the world. Enormous efforts are made all over the world to document as well as use these resources. Everybody knows that the introgression of genes in wheat provided the foundation for the "Green Revolution". Later also demonstrated the great impact that genetic resources have on production. Several factors are contributing to high plant performance under different environmental conditions, therefore an effective and complementary use of all available technological tools and resources is needed to meet the challenge.

"Cereal Genomics" published in 2004 served the purpose of collecting all information on cereal genomics at one place and was well received by the cereal workers through-out the world. The last eight years have witnessed significant advancement in the field of cereal genomics. For instance, high-density genetic maps, physical maps, QTL maps and even draft genome sequence have become available for several cereal species. Furthermore, the next generation sequencing (NGS) technologies have revolutionized genomics research, so that it is possible now to sequence genomes of hundreds or thousands of accessions of an individual cereal crop. Significant amounts of data generated using these NGS technologies created a demand for computational tools to analyse this massive data. In view of these developments, the Editors realised that there was a need to have an updated volume on the present status and future prospects of cereal genomics. These developments related to technology and the tools have been documented in this volume, thus supplementing our earlier edited volume "Cereal Genomics". "Cereal Genomics II" discusses advances in cereal genomics research made during the last eight years, and presents state-of-art cereal genomics and its utilization involving both basic research such as comparative genomics and functional genomics, and applied research like QTL mapping and molecular breeding.

The book provides an overview of current trends in biotechnology and medicinal plant sciences. The work includes detailed chapters on various advance biotechnological tools involved in production of phytoactive compounds of medicinal significance. Some recent and novel research studies on therapeutic applications of different medicinal plants from various geographical regions of the world have also been included. These studies report the antimicrobial activity of various natural plant products against various pathogenic microbial strains. Informative chapters on recent emerging applications of plant products such as source for nutraceuticals and vaccines have been integrated to cover latest advances in the field. This book also explores the conservation aspect of medicinal plants. Thus, chapters having comprehensively complied in vitro conservation protocols for various commercially important rare, threatened and endangered medicinal plants were provided in the present book.

Forest tree breeding has been ongoing for more than 70 years across Europe. It has successfully generated improved varieties for the major economical forest tree species. They are part of the present European forestry landscape and largely contribute to intensive wood production and other forest activities. In this book, we describe the state-of-art of breeding for the main forest tree species. We provide a comprehensive, unique and up-to-date overview of the major scientific results and breeding achievements gathered from the many programmes scattered across Europe. The book is divided into 10 chapters, each as a monograph corresponding to a species or group of species Abies spp., (Larix spp., Picea abies, Picea sitchensis, Pinus sylvestris, Pseudotsuga menziesii, and Mediterranean pines; Acer pseudoplatanus, Fraxinus excelsior, and Prunus avium). Each of them is written by a group of experts and focuses on the distribution and economical importance of the species; motivation for breeding and breeding objectives; intraspecific genetic variability, breeding populations and breeding strategy; forest reproductive material deployment including mass-propagation and, prospects and perspectives for joint research and breeding. The book is a unique and up-dated source of information for students, researchers and professionals interested in the genetics and domestication of forest tree species.

This important reference is the first comprehensive resource worldwide that reflects research achievements in neglected and underutilized crop biotechnology, documenting research events during the last three decades, current status, and future outlook. This book has 16 chapters divided into 4 sections. Section 1 has three chapters dealing with Chenopodium as a potential food source, thin cell layer technology in micropropagation of Jatropha, and Panax vietnamensis. Section 2 deals with molecular biology and physiology of Haberlea rhodopensis, cell trait prediction in vitro and in vivo of legumes, and application of TILLING in orphan crops. Section 3 has five chapters on biotechnology of neglected oil crops, Quinoa, Erucia sativa, Stylosanthes, and Miscanthus. And Section 4 contains five chapters mainly on genetic transformation of Safflower, Jatropha, Bael, and Taro. This section also includes a chapter on genetic engineering of Mangroves.

The study of origin and domestication of legumes described in this book emerged when it became apparent that while this kind of information is adequate for cereals, the pulses lagged behind. At the end of the 1960s the senior author initiated a study on the chickpea's wild relatives followed by similar attempts for broad bean, fenugreek, common vetch, bitter vetch, and lentil. The junior author joined the project in the late 1980s with a study of the genetics of interspecific hybrid embryo abortion in lentil and later has extensively investigated chickpea domestication and wild peas. While this book mainly describes our research findings, pertinent results obtained by others are also discussed and evaluated. Studying the wild relatives of legumes included evaluation of their taxonomic status, their morphological variation, ecological requirements, exploration of their distribution, and seed collection in their natural habitats. Seeds were examined for their protein profile as preliminary hints of their affinity to the cultigens and plants grown from these seeds were used for establishing their karyotype, producing intra- and interspecific hybrids and analyses of their chromosome pairing at meiosis and fertility. The aim of these investigations was the identification of the potential wild gene pool of the domesticated forms. Assessment of genetic variation among accessions, particularly in the genus Lens, was made by isozymes and chloroplast DNA studies. The main findings include the discovery of the chickpea wild progenitor; studies of lentil in three crossability groups; wild peas proceeded in two lines of study; faba bean and fenugreek and their wild progenitors have not yet been identified; common vetch and its related form were treated here as an aggregate (A. sativa); we found gene flow between members of different karyotypes is possible; bitter vetch and its relation to the domesticated form were established by breeding experiments.

"Diagnostics in Plant Breeding" is systematically organizing cutting-edge research reviews on the development and application of molecular tools for the prediction of plant performance. Given its significance for mankind and the available research resources, medical sciences are leading the area of molecular diagnostics, where DNA-based risk assessments for various diseases and biomarkers to determine their onset become increasingly available. So far, most research in plant genomics has been directed towards understanding the molecular basis of biological processes or phenotypic traits. From a plant breeding perspective, however, the main interest is in predicting optimal genotypes based on molecular information for more time- and cost-efficient breeding schemes. It is anticipated that progress in plant genomics and in particular sequence technology made recently will shift the focus from "explanatory" to "predictive" in crop science. This book assembles chapters on all areas relevant to development and application of predictive molecular tools in plant breeding by leading authorties in the respective areas.

Sebacinales have emerged as a fascinating order with mutualistic plant-fungal symbionts that consists of exclusively beneficial fungi. This volume of Soil Biology presents an overview of the current results in Sebacinales research with a focus on the potential of these fungi in crop improvement and stress tolerance. The authors demonstrate that Sebacinales are not only extremely versatile in their associations with roots, but are also almost universally present as symptomless endophytes. With this extraordinary diversity, Sebacinales with the key fungus Piriformospora indica might possess remarkable significance in natural ecosystems. Their biotechnological applications are expected to improve the quality of crops while maintaining ecologically and economically sustainable production systems.

Climate change is expected to have a drastic impact on agronomic conditions including temperature, precipitation, soil nutrients, and the incidence of disease pests, to name a few. To face this looming threat, significant progress in developing new breeding strategies has been made over the last few decades. The first volume of Genomics and Breeding for Climate-Resilient Crops presents the basic concepts and strategies for developing climate-resilient crop varieties. Topics covered include: conservation, evaluation and utilization of biodiversity; identification of traits, genes and crops of the future; genomic and molecular tools; genetic engineering; participatory and evolutionary breeding; bioinformatics tools to support breeding; funding and networking support; and intellectual property, regulatory issues, social and political dimensions.

Climate change is expected to have a drastic impact on agronomic conditions including temperature, precipitation, soil nutrients, and the incidence of disease pests, to name a few. To face this looming threat, significant progress in developing new breeding strategies has been made over the last few decades. The second volume of Genomics and Breeding for Climate-Resilient Crops describes various genomic and breeding approaches for the genetic improvement of the major target traits. Topics covered include: flowering time; root traits; cold, heat and drought tolerance; water use efficiency; flooding and submergence tolerance; disease and insect resistance; nutrient use efficiency; nitrogen fixation; carbon sequestration; and greenhouse gas emissions.

The book is about the seed development in the model and crop plants. Seed development is a key step of the plant life cycle that determines the nutrient value of seeds - the life for human civilization, growth, and development. The nutrient value of seeds is mainly due to storage reserve products such as carbohydrates, lipids (triacylglycerols), and proteins. The book primarily focuses on application of the 21st century high-throughput technologies transcriptomics, proteomics, metabolomics, and systems biology in near complete understanding of the various processes involved in seed development in different crop plants. The book reveals how such technologies have revolutionized our understanding of the multilayer processes and regulations involved therein by generating large-scale datasets. Accumulated datasets provide basic knowledge to develop integrated strategies to eventually improve the nutritional value of plant seed and crop yield, a critical goal in food security issues around the globe.

Microalgae are one of the most studied potential sources of biofuels and bioenergy. This book covers the key steps in the production of renewable biofuels from microalgae - strain selection, culture systems, inorganic carbon utilisation, lipid metabolism and quality, hydrogen production, genetic engineering, biomass harvesting, extraction. Greenhouse gas and techno-economic modelling are reviewed as is the 100 year history of microalgae as sources of biofuels and of commercial-scale microalgae culture. A summary of relevant basic standard methods used in the study of microalgae culture is provided. The book is intended for the expert and those starting work in the field.

This book was written in response to significant recent advances in understanding the mechanisms of parasitism in the Orobanchaceae, and breakthroughs in the control of the parasitic weeds Striga and Orobanche. It consists of 26 contributions by internationally recognized leading scientists. The main book chapters are grouped into two parts: * Part I - The Orobanchaceae and Their Parasitic Mechanisms * Part II - The Weedy Orobanchaceae and Their Control The first part provides cutting-edge information on all key aspects of plant parasitism, such as the structure, development and function of the haustorium; nutrient transfer and the physiology of the parasite-host association; host reaction to parasitic plants; seed production and germination; the strigolactones and host-parasite signaling mechanisms; the parasite genome, phylogenetics, evolution and epigenetics; and ecology. Topics of the second part include: the problem posed by the weedy parasites; population diversity and dynamics; molecular diagnosis of seed banks; and detailed discussion of the various management strategies, including agronomic, chemical and biotechnological approaches, as well as host breeding for resistance, allelopathy and biological control. This book is intended for plant scientists, university lecturers and students, agronomists and weed specialists, breeders and farmers, extension personnel and experts in tropical and subtropical agriculture.

Agro-industrial wastes are end-products emerging after industrial processing operations and also from their treatment and disposal e.g. solid fruit wastes and sludge. The agro-industrial wastes are often present in multiphase and comprise multicomponent. Nevertheless, these wastes are a goldmine as they possess valuable organic matter which can be diverted towards high value products ranging from polymers to antibiotics to platform chemicals. There have been plenty of books published on bioenergy, enzymes and organic acids, among others. However, this emerging field of biochemical has not yet been covered so far which is an important entity of the biorefinery model from waste biomass and needs to be understood from fundamental, applied as well as commercial perspective which has been laid out in this book.

Manyexcitingdiscoveriesinrecentdecadeshavecontributednewknowledgeto ourunderstandingofthemechanismsthatregulatevariousstagesofplantgrowth anddevelopment. Suchinformation,coupledwithadvancesincellandmolecular biology,isfundamentaltocropimprovementusingbiotechnologicalapproaches. Twovolumesconstitutethepresentwork. The?rst,comprising22chapters, commenceswithintroductionsrelatingtogeneregulatorymodelsforplantdev- opmentandcropimprovement,particularlytheuseofArabidopsisasamodelplant. Thesechaptersarefollowedbyspeci?ctopicsthatfocusondifferentdevelopmental aspectsassociatedwithvegetativeandreproductivephasesofthelifecycleofa plant. Six chapters discuss vegetative growth and development. Their contents considertopicssuchasshootbranching,buddormancyandgrowth,thedevel- ment of roots, nodules and tubers, and senescence. The reproductive phase of plantdevelopmentisin14chaptersthatpresenttopicssuchas?oralorganinit- tionandtheregulationof?owering,thedevelopmentofmaleandfemalegametes, pollengerminationandtubegrowth,fertilization,fruitdevelopmentandripening, seed development, dormancy, germination, and apomixis. Male sterility and self-incompatibilityarealsodiscussed. Volume2has20chapters,threeofwhichreviewrecentadvancesinsomatic embryogenesis,microsporeembryogenesisandsomaclonalvariation. Sevenofthe chapterstargetplantprocessesandtheirregulation,includingphotosynthatepartiti- ing,seedmaturationandseedstorageproteinbiosynthesis,theproductionandregu- tionoffattyacids,vitamins,alkaloidsand?owerpigments,and?owerscent. This secondbookalsocontainsfourchaptersonhormonalandenvironmentalsignaling (aminocompounds-containinglipids,auxin,cytokinin,andlight)intheregulationof plantdevelopment;othertopicsencompassthemoleculargeneticsofdevelopmental regulation,includingRNAsilencing,DNAmethylation,epigenetics,activationt- ging,homologousrecombination,andtheengineeringofsyntheticpromoters. Thesebookswillserveaskeyreferencesforadvancedstudentsandresearchers involved in a range of plant-orientated disciplines, including genetics, cell and molecularbiology,functionalgenomics,andbiotechnology. August2009 E-C. PuaandM. R. Davey v Contents PartI CellDifferentiationandDevelopmentInVitro 1 DevelopmentalBiologyofSomaticEmbryogenesis ...3 R. J. Rose,F. R. Mantiri,S. Kurdyukov,S-K. Chen,X-D. Wang, K. E. Nolan,andM. B. Sheahan 1. 1 Introduction ...3 1. 2 BasicRequirementsforInVitroSE ...4 1. 3 ExplantandStemCellBiology ...5 1. 3. 1 Genotype ...5 1. 3. 2 ExplantCells ...6 1. 4 EarliestEventinEmbryogenesis-AsymmetricCellDivision ...8 1. 4. 1 CellWallinEstablishmentofPolarity,DivisionAsymmetry andCellFate ...8 1. 4. 2 DivisionAsymmetryintheInitiationofSE ...10 1. 4. 3 AsymmetricDivisionandtheSuspensorinSE ...10 1. 5 StressComponentintheInitiationofSE ...11 1. 5. 1 ReactiveOxygenSpecies ...11 1. 5. 2 Stress-RelatedHormoneSignalling ...12 1. 6 HormonesandtheInitiationofSE ...13 1. 7 InductionofSEbyOver-ExpressionofLeafyCotyledon TranscriptionFactorsandTheirRelationshiptoSEInductionand Repression-theGAConnection ...14 1. 8 ABA,StressandGA ...16 1. 9 SolubleSignalsandCell-CellInteractionsthatPromoteSEin SuspensionCultures ...16 1. 9. 1 SecretedProteinsthatIn?uenceSE ...16 1. 9. 2 AGPSignallinginSE:MechanismsandInteractionsBetween SignallingPathways ...17 1. 9. 3 Cell-AsymmetricCellDivision ...8 1. 4. 1 CellWallinEstablishmentofPolarity,DivisionAsymmetry andCellFate ...8 1. 4. 2 DivisionAsymmetryintheInitiationofSE ...10 1. 4. 3 AsymmetricDivisionandtheSuspensorinSE ...10 1. 5 StressComponentintheInitiationofSE ...11 1. 5. 1 ReactiveOxygenSpecies ...11 1. 5. 2 Stress-RelatedHormoneSignalling ...12 1. 6 HormonesandtheInitiationofSE ...13 1. 7 InductionofSEbyOver-ExpressionofLeafyCotyledon TranscriptionFactorsandTheirRelationshiptoSEInductionand Repression-theGAConnection ...14 1. 8 ABA,StressandGA ...16 1. 9 SolubleSignalsandCell-CellInteractionsthatPromoteSEin SuspensionCultures ...16 1. 9. 1 SecretedProteinsthatIn?uenceSE ...16 1. 9. 2 AGPSignallinginSE:MechanismsandInteractionsBetween SignallingPathways ...17 1. 9. 3 Cell-Manyexcitingdiscoveriesinrecentdecadeshavecontributednewknowledgeto ourunderstandingofthemechanismsthatregulatevariousstagesofplantgrowth anddevelopment. Suchinformation,coupledwithadvancesincellandmolecular biology,isfundamentaltocropimprovementusingbiotechnologicalapproaches. Twovolumesconstitutethepresentwork. The?rst,comprising22chapters, commenceswithintroductionsrelatingtogeneregulatorymodelsforplantdev- opmentandcropimprovement,particularlytheuseofArabidopsisasamodelplant. Thesechaptersarefollowedbyspeci?ctopicsthatfocusondifferentdevelopmental aspectsassociatedwithvegetativeandreproductivephasesofthelifecycleofa plant. Six chapters discuss vegetative growth and development. Their contents considertopicssuchasshootbranching,buddormancyandgrowth,thedevel- ment of roots, nodules and tubers, and senescence. The reproductive phase of plantdevelopmentisin14chaptersthatpresenttopicssuchas?oralorganinit- tionandtheregulationof?owering,thedevelopmentofmaleandfemalegametes, pollengerminationandtubegrowth,fertilization,fruitdevelopmentandripening, seed development, dormancy, germination, and apomixis. Male sterility and self-incompatibilityarealsodiscussed. Volume2has20chapters,threeofwhichreviewrecentadvancesinsomatic embryogenesis,microsporeembryogenesisandsomaclonalvariation. Sevenofthe chapterstargetplantprocessesandtheirregulation,includingphotosynthatepartiti- ing,seedmaturationandseedstorageproteinbiosynthesis,theproductionandregu- tionoffattyacids,vitamins,alkaloidsand?owerpigments,and?owerscent. This secondbookalsocontainsfourchaptersonhormonalandenvironmentalsignaling (aminocompounds-containinglipids,auxin,cytokinin,andlight)intheregulationof plantdevelopment;othertopicsencompassthemoleculargeneticsofdevelopmental regulation,includingRNAsilencing,DNAmethylation,epigenetics,activationt- ging,homologousrecombination,andtheengineeringofsyntheticpromoters. Thesebookswillserveaskeyreferencesforadvancedstudentsandresearchers involved in a range of plant-orientated disciplines, including genetics, cell and molecularbiology,functionalgenomics,andbiotechnology. August2009 E-C. PuaandM. R. Davey v Contents PartI CellDifferentiationandDevelopmentInVitro 1 DevelopmentalBiologyofSomaticEmbryogenesis ...3 R. J. Rose,F. R. Mantiri,S. Kurdyukov,S-K. Chen,X-D. Wang, K. E. Nolan,andM. B. Sheahan 1. 1 Introduction ...3 1. 2 BasicRequirementsforInVitroSE ...4 1. 3 ExplantandStemCellBiology ...5 1. 3. 1 Genotype ...5 1. 3. 2 ExplantCells ...6 1. 4 EarliestEventinEmbryogenesis-AsymmetricCellDivision ...8 1. 4. 1 CellWallinEstablishmentofPolarity,DivisionAsymmetry andCellFate ...8 1. 4. 2 DivisionAsymmetryintheInitiationofSE ...10 1. 4. 3 AsymmetricDivisionandtheSuspensorinSE ...10 1. 5 StressComponentintheInitiationofSE ...11 1. 5. 1 ReactiveOxygenSpecies ...11 1. 5. 2 Stress-RelatedHormoneSignalling ...12 1. 6 HormonesandtheInitiationofSE ...13 1. 7 InductionofSEbyOver-ExpressionofLeafyCotyledon TranscriptionFactorsandTheirRelationshiptoSEInductionand Repression-theGAConnection ...14 1. 8 ABA,StressandGA ...16 1. 9 SolubleSignalsandCell-CellInteractionsthatPromoteSEin SuspensionCultures ...16 1. 9. 1 SecretedProteinsthatIn?uenceSE ...16 1. 9. 2 AGPSignallinginSE:MechanismsandInteractionsBetween SignallingPathways ...17 1. 9. 3 Cell-CellInteractionandRelevancetoSEinSuspension Cultures ...18 vii viii Contents 1. 10 DevelopmentProgramAfterSEInduction ...19 1. 11 ConcludingRemarksandaModelBasedonStudiesin Medicagotruncatula ...19 1. 12 SEandBiotechnology ...20 References ...21 2 MicrosporeEmbryogenesis ...27 A. Olmedilla 2. 1 Introduction ...27 2. 2 DiscoveryoftheProductionofHaploidsbyAntherCulture ...29 2. 3 StrategiesfortheInductionofMicrosporeEmbryogenesis ...29 2. 4 In?uenceofDifferentFactorsinMicrosporeEmbryogenesis ...30 2. 4. 1 Genotype ...30 2. 4. 2 DonorPlantPhysiology ...31 2. 4. 3 StageofPollenDevelopment ...

Crop production in greenhouses is a growing industry, especially in mild climates, and is very important for the population as a source of income and clean, fresh food. Greenhouses create optimal climate conditions for crop growth and protect crops from outside pests. At the same time greenhouse production increases water use efficiency and makes integrated production and protection (IPP) possible. This book provides technical instructions for practice (what to do and what not to do) and gives answers to the question: How to produce more clean crops and better quality with less water, less land and less pesticide. Suitable greenhouse constructions and their design, adapted to local climates in subtropical, tropical and arid regions and infrastructure conditions are presented. The necessary climate control measures - light transmittance, ventilation, cooling, heating, and CO2 enrichment - and physical measures for pest control, as well as methods for using solar energy to desalinate salty water are described. The results of theoretical research are transferred into methods for practical use, so that readers are equipped to solve their problems in practice as well as to get stimulation for further research and development.