The book "STEM CELL THERAPY FOR ORGAN FAILURES" edited by Dr. S. Indumathi demonstrates the In Vitro and In vivo therapeutic strategies and applications of pre- and post-natal stem cells for treating the failures of various organ systems of our body in a wide perspective. It explores the past, present and the futuristic approach of the exciting field of stem cells and its intriguing properties involved in tissue repair and regeneration. The prime focus of this volume is to unravel the basic, advanced, therapeutic and translational approaches put-forth so far in the field of stem cells and regenerative medicine at research, pre-clinical and clinical levels. Stem cells has ushered in widespread interest and exciting possibilities for cell based therapies, albeit failures do prevail and small uncontrolled phase I/II studies are only signals generating, rather than definite proof of concept thereby limiting its applicability in curative therapeutics. Despite certain initiatives and meticulous untiring efforts, bringing this basic bench side research into advanced transitional bedside remained a challenge. Thus, this book embarked upon the expanding researches in these areas that seem decisive in improvising regenerative medical therapeutics, thereby leading to further path-breaking studies that cure all health challenges facing mankind. Overall, this book reveals the imperativeness of various stem cell sources and its utility in curative therapeutics.

This second edition volume expands on the previous edition with discussions of recently developed techniques that use RNA scaffolds as molecular tools. These latest methods are used in the fields of molecular biology, cellular biology, nanotechnology, and structural biology. The chapters in this book cover topics such as RNA footprinting using small chemical reagents; using tRNA scaffolds to assist RNA crystallization; identification of RNA-binding proteins associated to RNA structural elements; and synthetic biology medicine and bacteria-based cancer therapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Scaffolds: Methods and Protocols, Second Edition is a valuable resource that explores the fascinating uses of RNA in these different areas, and will inspire experts and novice researchers to further study this developing field.

This open-access textbook provides a comprehensive, up-to-date guide for students and practitioners wishing to access in a single volume the key disciplines and principles of wheat breeding. Wheat is a cornerstone of food security: it is the most widely grown of any crop and provides 20% of all human calories and protein. The authorship of this book includes world class researchers and breeders whose expertise spans cutting-edge academic science all the way to impacts in farmers' fields. The book's themes and authors were selected to provide a didactic work that considers the background to wheat improvement, current mainstream breeding approaches, and translational research and avant garde technologies that enable new breakthroughs in science to impact productivity. While the volume provides an overview for professionals interested in wheat, many of the ideas and methods presented are equally relevant to small grain cereals and crop improvement in general. The book is affordable, and because it is open access, can be readily shared and translated -- in whole or in part -- to university classes, members of breeding teams (from directors to technicians), conference participants, extension agents and farmers. Given the challenges currently faced by academia, industry and national wheat programs to produce higher crop yields --- often with less inputs and under increasingly harsher climates -- this volume is a timely addition to their toolkit.

Clinical Decision Support for Pharmacogenomic Precision Medicine: Foundations and Implementation offers overviews, methods and strategies for translating genomic medicine to clinical practice. The book's authors explore incorporating pharmacogenetics into electronic health records, CDS methods and infrastructure for delivery, economic evaluation, the hospital administrations' role and needs in integration, and patient counseling aspects. The book empowers clinicians, researchers, translational scientists, and data and IT experts to effectively navigate the complex landscape of CDS for pharmacogenomic precision medicine. Illustrative case studies of existing gene networks include CSER, eMERGE, the IGNITE network, DIGITIZE, the CDS Learning Network (RTI), ClinGen, Ubiquitous and CDS Hooks.

Author Prof Jim Lynch is widely known and very experienced, well-connected with wide international networks, and globally distinguished. He was awarded the UNESCO Prize in Microbiology and Einstein Medal and Distinguished Scientist of the US Department of Agriculture. His OUP textbook Soil Microbiology was a bestseller. Taps directly into the topical area of the COVID-19 pandemic and One Health. Affordable and accessible to a wide interdisciplinary audience.

Genomic Medicine Skills and Competencies discusses core and practical aspects of genetic and genomic education and training for medical field. Many aspects of genomic applications in science, biotechnology, clinical medicine and healthcare require core and specialist knowledge, skills development and competencies for carrying out diverse tasks. Several knowledge-based courses and opportunities for skills and competencies development and assessment are now available and the main required subjects are discussed in this volume. The book focuses on all major aspects of genetic and genomic education training that are currently offered and evaluated and is a valuable resource for researchers, clinicians, physicians, nurses, genetic counselors, bioinformatics technicians, and other professionals who are interested in learning more about such promising field.

This volume details protocols on rationale design of therapeutic siRNA molecules and its encapsulation with smart vehicles to overcome the barriers to an effective administration in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Design and Delivery of SiRNA Therapeutics aims to ensure successful results in the further study of this vital field.

This second edition provides a comprehensive review of various gene silencing methodologies and applications. Chapters detail a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS), understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, artificial miRNAs for gene silencing, and high throughput RNAi. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Gene Silencing: Methods and Protocols, Second Edition aims to further the understanding of functional relevance of target genes using gene silencing methods and technologies in commercial plant varieties.

Drawing on their extensive teaching experience, the authors bring the content to life using humorous and engaging language and show students how the principles of behavior relate to their everyday lives. The text's tried-and-true pedagogy make the content as clear as possible without oversimplifying the concepts. Each chapter includes study objectives, key terms, and review questions that encourage students to check their understanding before moving on, and incorporated throughout the text are real-world examples and case studies to illustrate key concepts and principles.This edition also features a new full-color design and nearly 400 color figures, tables, and graphs. The text is carefully tailored to the length of a standard academic semester and how behavior analysis courses are taught, with each section corresponding to a week's worth of coursework, and each chapter is integrated with the task list for Behavior Analyst Certification Board (BACB) certifications.

In the five years since the publication of Molecular Systematics of Plants, the field of molecular systematics has advanced at an astonishing pace. This period has been marked by a volume of new empirical data and advances in theoretical and analytical issues related to DNA. Comparative DNA sequencing, facilitated by the amplification of DNA via the polymerase chain reaction (PCR), has become the tool of choice for molecular systematics. As a result, large portions of the Molecular Systematics of Plants have become outdated. Molecular Systematics of Plants II summarizes these recent achievements in plant molecular systematics. Like its predecessor, this completely revised work illustrates the potential of DNA markers for addressing a wide variety of phylogenetic and evolutionary questions. The volume provides guidance in choosing appropriate techniques, as well as appropriate genes for sequencing, for given levels of systematic inquiry. More than a review of techniques and previous work, Molecular Systematics of Plants II provides a stimulus for developing future research in this rapidly evolving field. Molecular Systematics of Plants II is not only written for systematists (faculty, graduate students, and researchers), but also for evolutionary biologists, botanists, and paleobotanists interested in reviewing current theory and practice in plant molecular systematics.

This book is the first comprehensive compilation of deliberations on domestication, genetic and genomic resources, breeding, genetic diversity, molecular maps & mapping of important biotic stress as well as nutritional quality traits, genome sequencing, comparative genomics, functional genomics and genetic transformation. The economic, nutritional and health benefits especially antioxidants mediated antiaging effects of finger millet are also discussed. It also presents the input use efficiency, wide adaptation, post-harvest processing and value addition of the crop. Altogether, the book contains about 300 pages over 16 chapters authored by globally reputed experts on the relevant field in this crop. This book is useful to the students, teachers and scientists in the academia and relevant private companies interested in genetics, pathology, molecular genetics and breeding, genetic engineering, structural and functional genomics and nutritional quality aspects of the crop. This book is also useful to seed and pharmaceutical industries.

This book celebrates the dawn of the rye genomics era with concise, comprehensive, and accessible reviews on the current state of rye genomic research, written by experts in the field for students, researchers and growers. To most, rye is the key ingredient in a flavoursome bread or their favourite American whisky. To a farmer, rye is the remarkable grain that tolerates the harshest winters and the most unforgiving soils, befitting its legacy as the life-giving seed that fed the ancient civilisations of northern Eurasia. Since the mid-1900s, scientists have employed genetic approaches to better understand and utilize rye, but only since the technological advances of the mid-2010s has the possibility of addressing questions using rye genome assemblies become a reality. Alongside the secret of its unique survival abilities, rye genomics has accelerated research on a host of intriguing topics such as the complex history of rye's domestication by humans, the nature of genes that switch fertility on and off, the function and origin of accessory chromosomes, and the evolution of selfish DNA.

Cells of all living organisms have the ability to respond to altered nutritional conditions. They have developed mechanisms to sense nutrient availability and to produce appropriate responses, which involve changes in gene expression and the production or degradation of certain enzymes and other proteins. In recent years, the understanding of nutrient-induced signal transduction has greatly advanced and the emerging picture is that nutrient signalling mechanisms evolved early in evolution. This book provides a detailed presentation and comparison of the key nutritional regulatory mechanisms in lower as well as higher eukaryotes, written by recognised experts in this expanding field.

Bioinformatics in Agriculture: Next Generation Sequencing Era is a comprehensive volume presenting an integrated research and development approach to the practical application of genomics to improve agricultural crops. Exploring both the theoretical and applied aspects of computational biology, and focusing on the innovation processes, the book highlights the increased productivity of a translational approach. Presented in four sections and including insights from experts from around the world, the book includes: Section I: Bioinformatics and Next Generation Sequencing Technologies; Section II: Omics Application; Section III: Data mining and Markers Discovery; Section IV: Artificial Intelligence and Agribots. Bioinformatics in Agriculture: Next Generation Sequencing Era explores deep sequencing, NGS, genomic, transcriptome analysis and multiplexing, highlighting practices forreducing time, cost, and effort for the analysis of gene as they are pooled, and sequenced. Readers will gain real-world information on computational biology, genomics, applied data mining, machine learning, and artificial intelligence. This book serves as a complete package for advanced undergraduate students, researchers, and scientists with an interest in bioinformatics.

Agricultural Nanobiotechnology: Biogenic Nanoparticles, Nanofertilizers and Nanoscale Biocontrol Agents presents the most up-to-date advances in nanotechnology to improve the agriculture and food industry with novel nanotools for the controlling of rapid disease diagnostic and enhancement of the capacity of plants to absorb nutrients and resist environmental challenges. Highlighting the emerging nanofertilizers, nanopesticides and nanoherbicides that are being widely explored in order to overcome the limitations of conventional agricultural supplements, the book provides important insights to enable smart, knowledge-driven selection of nanoscale agricultural biomaterials, coupled with suitable delivery approaches and formulations will lead to promising agricultural innovation using nanotechnology. Agricultural Nanobiotechnology: Biogenic Nanoparticles, Nanofertilizers and Nanoscale Biocontrol Agents explores emerging innovations in nanobiotechnology for agriculture, food, and natural resources to address the challenges of food security, sustainability, susceptibility, human health, and healthy life. The book is ideal for the multidisciplinary scientists whose goal is to see the use of nanomaterials in agriculture to reduce the amount of spread chemicals, minimize nutrient losses in fertilization and to generate increased yield through pest and nutrient management.

The book will discuss the molecular mechanisms of cancer diseases, stem cell proliferation and transformation into cancer cells beyond the physiological processes that occur in normal stem cell biology. Some of the key oncogenic events in cancer and their signaling pathways that regulate cell division cycle progression will be described considering prospects for using such knowledge in advanced cancer therapy. Each chapter shall provide an invaluable resource for information on the most current advances in the field, with discussion of controversial issues and areas of emerging importance

Applied Genomics and Public Health examines the interdisciplinary and growing area of how evidence-based genomic knowledge can be applied to public health, population health, healthcare and health policies. The book gathers experts from a variety of disciplines, including life sciences, social sciences, and health care to develop a comprehensive overview of the field. In addition, the book delves into subjects such as pharmacogenomics, genethics, big data, data translation and analysis, economic evaluation, genomic awareness and education, sociology, pricing and reimbursement, policy measures and economic evaluation in genomic medicine. This book is essential reading for researchers and students exploring applications of genomics to population and public health. In addition, it is ideal for those in the biomedical sciences, medical sociologists, healthcare professionals, nurses, regulatory bodies and health economists interested in learning more about this growing field.

This contributed volume aims at bringing together all the genetic engineering tools for managing various types of crop pests. The main focus of this book is to explore the application of these tools in pest management. Major pest groups covered in this book are insects, mites and nematodes.The first section covers all major genetic tools and molecular approaches. The second section deals with genetic tools for of beneficial containing three chapters involving honey bees, silkworms and natural enemies. Next section deals with genetic interactions against pests in diverse geographical regions with special focus on Africa, Vietnam and Sri Lanka. Sections four and five addresses diverse aspects as management of pests, genetic behavior, gene expression, plasticity, pathways and interactions and options for mitigation of pests.It serves as a useful resource for professionals in the fields of entomology, agronomy, horticulture, ecology, and environmental sciences, as well as to agricultural producers and plant biotechnologists.

This book provides an update on the latest development in the field of microRNAs in cancer research with an emphasis on translational research. Since the early 2000s, microRNAs have been recognized as important and ubiquitous regulators of gene expression. Soon it became evident that their deregulation can cause human diseases including cancer. This book focuses on the emerging opportunities for the application of microRNA research in clinical practice. In this context, computer models are presented that can help to identify novel biomarkers, e.g. in circulating microRNAs, and tools that can help to design microRNA-based therapeutic interventions. Other chapters evaluate the role of microRNAs in immunotherapy, immune responses and drug resistance. Covering key topics on microRNAs in cancer research this book is a valuable resource for both emerging and established microRNA researchers who want to explore the potential of microRNAs as therapeutic targets or co-adjuvants in cancer therapies.

Genomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers. In Genomic Imprinting: Methods and Protocols, experts in the field provide a survey of the technologies that are being applied to advance the study of imprinting. This detailed volume features new technologies that are accelerating the pace of discovery of imprinted genes and characterization of their epigenetic profile, bioinformatic procedures for prediction and comparative analyses of imprinted genes, as well as methods in embryology and basic molecular biology that have been employed for many years, some appearing in new versions for small cell numbers. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Genomic Imprinting: Methods and Protocols will aid scientists in unveiling both much awaited answers and all-new questions to keep this vital field busy for many exciting years to come.

Now in its second edition, this book provides a state of the art overview on basic concepts of epigenetic epidemiology and a comprehensive review of the rapidly evolving field of human epigenetics. Epigenetics plays an important role in shaping who we are and contributes to our prospects of health and disease. Unlike our genetic inheritance, our epigenome is malleable throughout the lifecourse and is shaped by our environmental experiences. Population-based epidemiologic studies increasingly incorporate epigenetic components. These so called epigenome-wide association studies (EWAS) contribute substantially to our understanding of the relevance of epigenetic marks, such as DNA methylation, histone modification, and non-coding RNAs for disease causation. Written by leading experts in the field, the book opens with a comprehensive introduction of the principles of epigenetic epidemiology and discusses challenges in study design, analysis, and interpretation. It summarizes the latest advances in epigenetic laboratory techniques, the influence of age and environmental factors on shaping the epigenome, the epigenetic clock, and the role of epigenetics in the developmental origins hypothesis. The final part focuses on epigenetic epidemiology of various health conditions such as imprinting disorders, cancer, infectious diseases, inflammation and rheumatoid arthritis, asthma, metabolic disorder and vascular disease, as well as neurodevelopmental and psychiatric disorders. Given its scope, Epigenetic Epidemiology is an indispensable resource for researchers working in the field of human epigenetics.

This book illustrates the importance and significance of regenerative medicine in stroke recovery. It discusses stem-cell-based treatment strategies and offers mechanistic insights into their role in neurological recovery. It also examines the challenges and advances in using adult stem cells for enhanced therapeutic efficacy. Further, it presents the strategies as well as the strengths and weaknesses of various delivery methods to administer stem cells in ischemic stroke. It examines the role of non-coding RNA in our understanding the stroke pathogenesis, their regulatory role in ischemic stroke and potential as biomarkers and therapeutic targets. Lastly, it explores exosomes in the treatment of stroke, and the underlying mechanism of their action as therapeutic vectors for stroke. Given its scope, it is an excellent resource for neurologists, neuroscientists and researchers involved in regenerative therapy for stroke.