Bioactive compounds produced by natural sources, such as plants, microbes, endophytic fungi, etc., can potentially be applied in various fields, including agriculture, biotechnology and biomedicine. Several bioactive compounds have proved to be invaluable in mediating plant-microbe interactions, and promoting plant growth and development. Due to their numerous health-promoting properties, these compounds have been widely used as a source of medication since ancient times. However, there is an unprecedented need to meet the growing demand for natural bioactive compounds in the flavor and fragrance, food, and pharmaceutical industries. Moreover, discovering new lead molecules from natural sources is essential to overcoming the rising number of new diseases. In this regard, natural bioactive compounds hold tremendous potential for new drug discovery. Therefore, this field of research has become a vital area for researchers interested in understanding the chemistry, biosynthetic mechanisms, and pharmacological activities of these bioactive metabolites. This book describes the basics of bioactive plant compounds, their chemical properties, and their pharmacological biotechnological properties with regard to various human diseases and applications in the drug, cosmetics and herbal industries. It offers a valuable asset for all students, educators, researchers, and healthcare experts involved in agronomy, ecology, crop science, molecular biology, stress physiology, and natural products.

This book is an overview of primary sensory maps of vertebrates, characterized by continuous and discrete properties. The eight primary sensory maps of vertebrates have unique features and use distinct molecular cues, cell cycle exit, and activity combinations during development, regeneration, and plasticity. As an introduction and overview, the book provides a short overview for all eight sensory senses and presents through evolution and gene regulatory networks, the molecular cues needed for sensory processing. Independent contributions are included for olfactory, vision, trigeminal, taste, vestibular, auditory, lateral line, and electroreception.

This volume examines the international impact of Lysenkoism in its namesake's heyday and the reasons behind Lysenko's rehabilitation in Russia today. By presenting the rise and fall of T.D. Lysenko in its various aspects, the authors provide a fresh perspective on one of the most notorious episodes in the history of science.

This monograph introduces current genome editing technologies-clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated (Cas) systems, transcription activator-like effector nucleases (TALENs), and zinc-finger nucleases (ZFNs)-and provides an assessment of the risk of misuse of these technologies based on the following parameters: accessibility, ease of misuse, magnitude of potential harm, and imminence of potential misuse. The findings from this assessment are applied to analyze and evaluate the threat posed by the intentional misuse of genome editing technologies to develop biological weapons. Furthermore, the book discusses the implications of misuse for different applications of genome editing, such as making existing pathogens more dangerous, modifying the human microbiome, weaponizing gene drives, engineering super soldiers, and augmenting the general population to confer economic advantages. Technologies that enable genome editing with programmable nucleases-including CRISPR, TALEN, and ZFN-allow for the precise genetic modification of organisms and cultured cells. While these technologies are used for a variety of beneficial applications, intelligence and defense experts have raised concerns that genome editing technologies, especially CRISPR, could be misused to develop new and improved biological weapons. Furthermore, experts worry that the number and type of actors who could potentially misuse genome editing is dramatically increasing given the democratization of biology, which is allowing biology to become more accessible to everyone including nonexperts. The book provides a comprehensive assessment of how feasible it is for users with different levels of knowledge and skill to acquire and then to apply the technologies to develop a biological weapon. It also provides an assessment of governability and a tailored set of recommendations that address security concerns. These recommendations are sensitive to the cost-benefit trade-off of regulating genome editing technologies. The book targets researchers as well as intelligence analysts, defense and security personnel, and policymakers.

This detailed book explores a diverse range of topics related to wound healing. Some areas include wound generation as a tool to study cellular interactions and growth, mechanisms by which different tissues regenerate, ischemic wounds and their treatment, repairing corneal wound, as well as a number of emerging technologies to help promote wound regeneration, such as techniques with miRNA, nanomaterials, biomaterials, and stem cell therapies. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Wound Regeneration: Methods and Protocols serves as a vital collection of wound model development and regeneration studies that will be of great use in both the academic and industrial fields of research.

This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.

Genetic Ancestry focuses on the scientific nature and limitations of genetic ancestry testing. Co-authored by a genetic anthropologist and a cultural anthropologist, it examines the social, historical, and cultural dimensions of how people interpret genetic ancestry data. Utilizing examples from popular culture around the world and case studies from the Caribbean, the chapters highlight how genetic technology can sometimes bolster racial thinking and serve as tool of resistance and social justice.

The Evolution of Human Cleverness presents a unique introduction to the way human cognitive abilities have evolved. The book comprises a series of mini-essays on distinct topics in which technical terms are simplified, considering how humans made the long journey from our ape-like ancestors to become capable of higher-level reasoning and problem solving. All the topics are cross-linked, allowing the reader to dip in and out, but certain key concepts run through the underlying reasoning. Chiefly, these are adaptation and selection, the distinction between ultimate and proximate causes of behaviour, gene-culture co-evolution, and domain-general versus domain-specific cognitive processes. The book should help the reader draw lessons for the human species as a whole, especially in view of the environmental threats to its own existence. Entries have been carefully crafted to cut through scientific jargon, providing bite-sized and digestible chunks of knowledge, making the topic accessible for students and lay readers alike. The author draws on research from diverse fields including Psychology, Anthropology, Archaeology, Biology, and Neuroscience to provide an unbiased account of the field, making it an ideal text for students of all levels.

Taking us to the cutting edge of the new frontier of medicine, a visionary biotechnologist and a pathbreaking researcher show how we can optimize our health in ways that were previously unimaginable. We are on the cusp of a major transformation in healthcare—yet few people know it. At top hospitals and a few innovative health-tech startups, scientists are working closely with patients to dramatically extend their “healthspan”—the number of healthy years before disease sets in. In The Age of Scientific Wellness, two visionary leaders of this revolution in health take us on a thrilling journey to this new frontier of medicine. Today, most doctors wait for clinical symptoms to appear before they act, and the ten most commonly prescribed medications confer little or no benefit to most people taking them. Leroy Hood and Nathan Price argue that we must move beyond this reactive, hit-or-miss approach to usher in real precision health—a form of highly personalized care they call “scientific wellness.” Using information gleaned from our blood and genes and tapping into the data revolution made possible by AI, doctors can catch the onset of disease years before symptoms arise, revolutionizing prevention. Current applications have shown startling results: diabetes reversed, cancers eliminated, Alzheimer’s avoided, autoimmune conditions kept at bay. This is not a future fantasy: it is already happening, but only for a few patients and at high cost. It’s time to make this gold standard of care more widely available. Inspiring in its possibilities, radical in its conclusions, The Age of Scientific Wellness shares actionable insights to help you chart a course to a longer, healthier, and more fulfilling life.

This volume explores novel concepts of pericyte biology. The present book is an attempt to describe the most recent developments in the area of pericyte biology which is one of the emergent hot topics in the field of molecular and cellular biology today. Here, we present a selected collection of detailed chapters on what we know so far about the pericytes. Together with its companion volumes Pericyte Biology in Different Organs and Pericyte Biology in Disease, Pericyte Biology - Novel Concepts presents a comprehensive update on the latest information and most novel functions attributed to pericytes. To those researchers newer to this area, it will be useful to have the background information on these cells' unique history. It will be invaluable for both advanced cell biology students as well as researchers in cell biology, stem cells and researchers or clinicians involved with specific diseases.

The European Molecular Biology Open Software Suite (EMBOSS) is a high quality package of open source software tools for molecular biology. It includes over 200 applications integrated with a range of popular third party software packages under a consistent and powerful command line interface. The tools are available from a wide range of graphical interfaces, including easy to use web interfaces and powerful workflow software. The EMBOSS Administrator's Guide is the official, definitive and comprehensive guide to EMBOSS installation and maintenance: * Find all the information needed to configure, install and maintain EMBOSS, including recent additions for version 6.2 * Step-by-step instructions with real-world examples - saves readers time and helps them avoid the pitfalls on all the common platforms * In-depth reference to database configuration - learn how to set up and use databases under EMBOSS * Includes EMBOSS Frequently Asked Questions (FAQ) with answers - quickly find solutions to common problems

This volume explores the latest techniques used for sustainable development of eco-friendly pest management systems. Chapters in this book look at the design of novel RNAi-mediated pest control strategies and related issues. Some of the topics covered in this book are identification and characterization of differentially expressed non-coding RNAs in insect genomes; functional small RNA design; analysis of endogenous RNAi pathways; RNAi screening; plant models of RNAi-mediated pest control; and dsRNA delivery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNAi Strategies for Pest Management: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this exciting and developing field. Chapter 18 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

The major purpose of this book is to present Johann Gregor Mendel (1822-1884) in a real and interesting way based on the most recent historical research and analysis of authentic sources. The authors aim to show Mendels scientific thinking and inner feelings together with his environment and to communicate his message as a multifaceted personality and modern experimentalist. The book draws from the only existing short sketch of Mendels youth, his letters and the biographical ceiling paintings that were made according to his proposal. They form the basis of the self-portrait concept. The structure of the book follows thematic groups covering Mendels activities from a poor village boy in search for education and financial security, as not being physically suitable for running his father's farm. The book does not perpetuate the myths invented by some creative authors to make Mendels biography more attractive. Mendels life and work are dramatic enough without those embellishments. Mendel found happiness in science and he was able to explain the theory of new scientific facts. He was not a tragic figure, he did not work to become famous, but to be useful. His pea research has now been appreciated as a genius accomplishment of a scientist. The book is published at the occasion of Mendels birthday bicentennial.

This is the first book in the field of mouse genetics to provide comprehensive and standardized methods for the characterization of laboratory mice. The editor is Director of the German Mouse Clinic and member of the Project Committee of the German National Genome Research Network and provides here a brief introduction to the mouse as a model for diseases and functional analysis of genes and proteins. Throughout, he focuses on the characterization of mouse models using the latest phenotyping methods, with the different areas presented in a clearly structured and easily accessible manner.

This book provides methods and techniques used in construction of global transcriptional regulatory networks in diverse systems, various layers of gene regulation and mathematical as well as computational modeling of transcriptional gene regulation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Modeling Transcriptional Regulation: Methods and Protocols aims to provide an in depth understanding of new techniques in transcriptional gene regulation for specialized audience.

This volume contains a comprehensive collection of laboratory protocols used by researchers to analyze varied aspects of non-alcoholic steatohepatitis (NASH). The chapters in this book cover topics such as methods for histological diagnosis of NASH; the purpose of generating an in vivo NASH model; protocols for isolating hepatocytes and Kupffer cells, bone marrow derived macrophages, and adipocytes; techniques to develop human pluripotent stem cells-derived liver organoids; single-cell and RNA-sequencing; and a description of how to extract exosomes and exosomal miRNAs from mesenchymal stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Non-Alcoholic Steatohepatitis: Methods and Protocols is a valuable resource for new and experiences investigators studying NASH, and serves as an essential reference on NASH for basic and clinical researchers and students.

1. Present more scientific insights that will be helpful for both scientists and healthcare professionals to control, diagnose, and treat COVID-19 2. Useful Scientist, academicians, physicians, aroma therapists, medical practitioners, researchers those are working in development of therapeutics for COVID-19 and other infectious diseases (especially virologists, pathologist, pharmacologist, pulmonologists, ayurvedic practitioners, molecular biologists, essential oil practitioners are consultant/educators), pharmacognosist 3. Demonstrates the role of essential oils and their chemical components in the management of COVID-19

This book, for biochemists and molecular biologists, presents the best and most recent computational tools and approaches for recognizing and analysing biological patterns such as those that occur in DNA, RNA, amino-acid sequences, molecular structural motifs, gene and protein families, and so on. These tools have largely been developed by computer scientists working in such areas as machine learning, computer vision, neural networks, graphics, data compression, statistics, and parallel computing, and a sizable proportion of the biological community needs help and guidance in biological informatics approaches to the rapidly growing databases of molecular and genetic information.

This new edition presents principle methods in capillary electrophoresis (CE) separation involving CZE, MEKC, MECC, NACE, and corresponding hyphenated techniques to organic mass spectrometry and ICP-MS. Recent developments in the techniques of single cell analysis, as well as derivation, enantioseparation or the use of ionic liquids, and the use of CZE for the separation of living cells are also highlighted. This book discusses various application methods for the analysis of small ions, organic acids, amino acids, and (poly)saccharides to peptides that are shown with pollutants and biomarkers in food and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Capillary Electrophoresis: Methods and Protocols, Second Edition covers a wide field of interests and will be especially great for beginners and students because of its combined focus on mini-reviews and application notes that will help them quickly get an overview of the field.

Computational thinking is increasingly gaining importance in modern biology, due to the unprecedented scale at which data is nowadays produced. Bridging the cultural gap between the biological and computational sciences, this book serves as an accessible introduction to computational concepts for students in the life sciences. It focuses on teaching algorithmic and logical thinking, rather than just the use of existing bioinformatics tools or programming. Topics are presented from a biological point of view, to demonstrate how computational approaches can be used to solve problems in biology such as biological image processing, regulatory networks, and sequence analysis. The book contains a range of pedagogical features to aid understanding, including real-world examples, in-text exercises, end-of-chapter problems, colour-coded Python code, and 'code explained' boxes. User-friendly throughout, Computational Thinking for Life Scientists promotes the thinking skills and self-efficacy required for any modern biologist to adopt computational approaches in their research with confidence.

Much research has focused on the basic cellular and molecular biological aspects of stem cells. Much of this research has been fueled by their potential for use in regenerative medicine applications, which has in turn spurred growing numbers of translational and clinical studies. However, more work is needed if the potential is to be realized for improvement of the lives and well-being of patients with numerous diseases and conditions.This book series 'Cell Biology and Translational Medicine (CBTMED)' as part of Springer Nature's long-standing and very successful Advances in Experimental Medicine and Biology book series, has the goal to accelerate advances by timely information exchange. Emerging areas of regenerative medicine and translational aspects of stem cells are covered in each volume. Outstanding researchers are recruited to highlight developments and remaining challenges in both the basic research and clinical arenas. This current book is the twelfth volume of a continuing series.

Leading gene therapy researchers and clinicians illuminate the field-from basic vector technology to current and future clinical applications in neurology. The authoritative contributors provide cutting-edge reviews of the vectors available for gene transfer to the central nervous system, the strategies against CNS tumors, the potential strategies against neurologic disorder, and the limitations of today's gene therapy approaches. Also discussed are significant applications of gene therapy to brain tumors, Parkinson's disease, ischemia, and Huntington's chorea. Readers will learn the current delivery methods for transgenes, will learn the characteristics of transgene delivery vectors, and come to understand the therapy for both neuro-oncologic and neurologic disorders.

This book aims to be an introduction to haplotype information for both the wet and dry labs. Chapters detail co-barcoding and linked-reads based methods, third generation sequencing based methods, Hi-C based methods, single-cell and Strand-seq, and methods for using haplotype data once obtained. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Haplotyping: Methods and Protocols aims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientists.

This book presents up-to-date information on the origins of the Ashkenazic Jewish people from central and eastern Europe based on genetic research on modern and pre-modern populations. It focuses on the 129 maternal haplogroups that the author confirmed that Ashkenazim have acquired from distinct female ancestors who were indigenous to diverse lands that include Israel, Italy, Poland, Germany, North Africa, and China, revealing both their Israelite inheritance and the lasting legacy of conversions to Judaism. Genetic connections between Ashkenazic Jews and other Jewish populations, including Turkish Jews, Moroccan Jews, Tunisian Jews, Iranian Jews, and Cochin Jews, are indicated wherever they are known.

Legumes include many very important crop plants that contribute very critical protein to the diets of both humans and animals around the world. Their unique ability to fix atmospheric nitrogen in association with Rhizobia enriches soil fertility, and establishes the importance of their niche in agriculture. Divided into two volumes, this work presents an up-to-date analysis of in vitro and recombinant DNA technologies for the improvement of grain, forage and tree legumes.
Volume 10A examines the current status and future prospects of challenges of the following: in vitro morphogenesis; biotic and abiotic stress tolerance; genomics; nitrogen fixation and utilization; nutritional improvement, and biodiversity of wild and tribal legumes.
Volume 10B presents the current state and future prospects of in vitro regeneration and genetic transformation expression and stability of transgenes modification of traits in almost all the important legumes, for example: soybean; peanut; pea; french bean; chick pea; pigeon pea; cowpea; mung bean; black gram; azuki bean; lentil; Lathyrus; lupinus; Lotus spp; Medicago spp; Trifolium spp; Winged bean; Guar; and tree legumes for their improvement. Written by international experts, these volumes will be of great value to researchers, as well as graduate students and all those requiring an advanced level overview of the subject area.