Key features: High quality full color photographs and descriptive texts on the location and removal of the organs from the mouse Instructive methods and clear visuals for trimming and orienting the organs for paraffin histology to obtain the best possible sections for analysis Full color photomicrographs of the resulting section for each organ stained with hematoxylin and eosin demonstrating important features and landmarks for the histologist to ensure the optimal area for analysis is achieved All in one, easy to use guide organized by individual organs of the laboratory mouse Spiralbound for easy reference in the lab This "one-stop" guide offers an essential resource for any academic, research or development operation where mouse necropsy and/or histology are performed. Connecting the reader 'from the mouse to the microscope', it provides a detailed guide for locating, trimming, orientating and embedding of the most frequently investigated tissues collected in the laboratory mouse. It shows where the organs reside in the mouse, how to trim and embed them as well as the resulting optimal sections. This guide brings together the wealth of scattered information into one high-quality text, the emphasis is on providing knowledge that will help histologists and scientists get better results in any downstream assays where ideal sections are needed.

The success of laboratory experiments relies heavily on the technical ability of the bench scientist, with the aid of "tricks-of-the-trade", to generate consistent and reliable data. Regrettably, however, these invaluable "tricks-of-the-trade" are frequently omitted from scientific publications. This paucity of practical information relating to the conduct of laboratory bacteriology experiments creates a gaping void in the pertinent literature. Methods in Practical Laboratory Bacteriology fills this void. It provides detailed technical information that ensures that you achieve consistent and reliable data. The book addresses the aspects of bacterial fractionation and membrane characterization, the analysis of Lipopolysaccharides and the techniques of SDS-PAGE, immunoblotting, and ELISA. It also describes the methods used for detecting and quantifying bacterial resistance to antibiotics, and the analysis of bacterial chromosomes by pulsed-field gel electrophoresis (PFGE). Methods in Practical Laboratory Bacteriology also covers protocols for extracting the fingerprinting plasmids, as well as the use of non-radio labeled gene probes and ribosomal RNA gene probes.

The spectacular advances of medicinal chemistry in the last few decades have been triggered by a greater understanding of cellular processes at the molecular level. The understanding of biochemical processes and diseases at molecular level has revolutionized the field. This volume summarizes recent developments in the area of biological inhibitors such as squalene epoxidase inhibitors, dual inhibitors of 5-lipoxygenase and cycloxgenase, inhibition of cholestrol biosynthesis, HIV proteinase inhibitors, nonpeptide antagonists at peptide receptors, and binding interaction of thyroid hormones.

Several excellent books have been published that address one or more aspects of the diverse field of industrial mycology, but none of them cover the entire process of fungal bioactive metabolites discovery. Until now. The Handbook of Industrial Mycology provides, in one volume, an overview of recent developments in industrial mycology with emphasis on the discovery of bioactive metabolites and, most importantly, their underlying biology and genetics. Two additional features distinguish this book from other books in the field: 1) most chapters are prepared using experimental data to illustrate theories and 2) the authors provide methodologies and experimental protocols in their chapters. Presenting a comprehensive overview of recent advances, the book provides a framework of basic methods, tools, and organizational principles for channeling fungal germplasm into the academic, pharmaceutical, and enzyme discovery laboratories. It covers the complex range of processes involved in the discovery, characterization, and profiling of bioactive fungal metabolites. The book includes examples of several recently marketed fungal metabolites and explores the impact of fungi on applications in the pharmaceutical, food and beverage, agricultural, and agrochemical industries.

Biotechnology revolutionized traditional plant breeding programs. This rapid change produced new discussions on techniques and opportunities for commerce, as well as a fear of the unknown. Plant Development and Biotechnology addresses the major issues of the field, with chapters on broad topics written by specialists. The book applies an informal style that addresses the major aspects of development and biotechnology with minimal references, without sacrificing information or accuracy. Divided into five primary parts , this volume explores how the field emerged from its early theoretical base to the technical discipline of today. It also covers progress being made with genetically engineered plants, providing a snapshot of the field's controversial present. Part III discusses methods for preparing media, creating solutions and dilutions, and accomplishing sterile culture work. It investigates common methods for visualizing and documenting studies, and quantifying responses of tissue culture in research.P art IV delivers the essential foundation of plant tissue culture, introducing the three types of commonly used culture regeneration systems.; Part V integrates propagation technique

With the advances in the field of molecular biology, new tools make it possible to conduct in-depth studies in food microbial communities from a molecular perspective. Information from genomic, transcriptomic, proteomic and metabolomic studies can be integrated through bioinformatic applications, thereby improving our understanding of the interactions between biotic and abiotic factors and concomitantly the physiology of starter cultures, spoilage and pathogenic microbiota. Improvements in the speed, accuracy and reliability of food quality and safety assessment have made the foundation stronger for future developments including the exploitation of gene networks and applications of nanotechnology and systems biology. This book reviews all these developments, provides an integrated view of the subject and helps in identifying areas of future development.

Facioscapulohumeral muscular dystrophy (FSHD) is a genetic disorder involving slowly progressive muscle degeneration in which the muscles of the face, shoulder blades and upper arms are among the most severely affected. It is the third most common inherited muscular dystrophy, affecting 1 in 20,000. The search for the molecular basis of the disease is of interest to all genetic researchers, involving a deletion outside a coding region resulting in over-expression of adjacent genes. This volume summarizes the current understanding of the disorder, including clinical, molecular and therapeutic aspects.

Since the publication of the best-selling first edition, much has been discovered about Saccharomyces cerevisiae, the single-celled fungus commonly known as baker's yeast or brewer's yeast that is the basis for much of our understanding of the molecular and cellular biology of eukaryotes. This wealth of new research data demands our attention and requires a reference that updates our current knowledge. Completely rewritten, updated, and expanded, Metabolism and Molecular Physiology of Saccharomyces cerevisiae, 2nd Edition provides a modern account of the metabolism and physiology of this important organism. All chapters have been thoroughly revised, and two new chapters on ageing and on the molecular organization and biogenesis of the cell wall have been added to highlight new areas of research. The book also includes coverage of morphogenesis, protein trafficking, lipids and membranes, and stress responses. Written by a team of internationally recognized experts, this authoritative second edition is a leading reference for bioscientists across many disciplines as well as novices interested in the latest developments in this complex field.

Contents:
1. Molecular Clocks: Whence and Whither 2. Molecular Clocks and a Biological Trigger for Neoproterozoic Snowball Earth Events and the Cambrian Explosion 3. Phylogenetic Fuses and Evolutionary 'Explosions': Conflicting Evidence and Critical Tests 4. The Quality of the Fossil record 5. Ghost Ranges 6. Episodic Evolution of Nuclear Small Subunit Ribosomal RNA Gene in the Stem-lineage of Foraminifera 7. Dating the Origin of Land Plants 8. Angiosperm Divergence Times: Congruence and Incongruence Between Fossils and Sequence Divergence Estimates 9. The Limitations of the Fossil Record and the Dating of the Origin of the Bilateria 10. The Origin and Early Evolution of Chordates: Molecular Clocks and the Fossil Record 11. Bones, Molecules and Crown-tetrapod Origins 12. The Fossil record and Molecular Clocks: Basal Radiations Within the Neornithes

Revised and expanded, this blue-ribbon reference emphasizes the latest developments in the identification, utilization, and analysis of flavonoids for the prevention of disease and maintenance of good health. The book examines the processes involved in the absorption, metabolism, distribution, and excretion of these compounds and the impact of biotransformation on flavonoid function.
The Second Edition contains new discussions on the potential of dietary flavonoids to attenuate neurological dysfunction and degeneration, developments in gene expression and genomics for identification of therapeutic targets and markers of disease, and the mechanisms regulating flavonoid bioavailability.

This book analyzes the nature of polio replication complexes with respect to subcellular fractionation, responses to detergent treatments, and the viral/cellular proteins involved. It presents models for both initiation and elongation of RNA chains by the poliovirus replicase.

Drugs are used in the diagnosis, alleviation, treatment, prevention or cure of disease. This is a book about drugs, how they came to be, and how they exert their 'magic'. Today we have drugs to protect against infectious diseases, to alleviate aches and pains, to allow new organs to replace the old, and for brain functions to be modified. Yet, for the most part the manner by which drugs are developed and by whom remains a mystery. Drugs are more than just a pill or liquid and some have markedly altered history. The author has selected a few drugs - highlights representing milestones affecting our well-being and influencers of social change. The stories told are dramatic and include spectacular successes and dismal failures. And the people about whom these stories are told are both saints and sinners - selfless and conniving - bold and mercurial and shy and retiring loner. The drugs themselves mirror the diversity of their origin stories and the author assembles all sides of these fascinating stories. Key Selling Features: Chronicles the development of some of the most significant drugs affecting human health Offers easy-to-follow and understandable explanations of how drugs work Explains the science underlying the processes of drug discovery Helps readers appreciate the contributions of the individuals involved in drug discovery and development Serves as essential reading for anyone interested in human health and public policy

This book contains forty reviewed papers delivered at the International Congress on Molecular Biology and Cultural Heritage held in Seville, March 2003. It is divided in four parts, the first one presents the state-of-the-art and reviews molecular techniques applied to the study of microbial communities colonizing monuments and cultural heritage assets. Part two covers specific molecular techniques used in biodetereoration studies, part three includes an updated overview on on-going biodetereoration European Commission projects, and part four presents selected biodetereoration case studies from all over the world.

The present volumes are based on the presentations made during the October 1981 research work shopThe attachment of micro-organisms to the intestinal mucosa which was held in Reston, Virginia. The workshop was supported by the American Gastroenterological Association, along with the National Institutes of Health.

This book reflects the different efforts made by veterinary and medical doctors for better knowledge of the disease. It shows how much we depends on each other to understand better the clinical features, pathogenesis, and epidemiology of campylobacter infections and other diseases.

This reference examines laboratory techniques and FDA and industry perspectives on medical, food service, and consumer product applications of antimicrobials. It offers methods to conduct investigations of effectiveness that simulate use of consumer, food, and medical antimicrobials in real-world conditions and environments, validate neutralizing systems, develop reliable and functional antimicrobial products, and prevent infection, disease, and contamination. Additional topics include the importance of customer-valued quality attributes, such as low skin irritation and ease of use, label claims, regulatory procedures, and quality control, and clinical simulation protocols.

Originally, it was our intention to produce a single-volume book covering all aspects and approaches to the problem of specific inhibitors of respiratory viruses. However, as the work progressed it became obvious that certain chapters, because of the research interests of the authors, concentrated particularly on influenza viruses. It seemed logical therefore, to divide the book into two volumes, the first emphasizing influenza and the second concentrating on other viruses as well as discussing important general aspects of drug screening and clinical testing, although the second volume does have some chapters which deal mainly with influenza.

Originally, it was our intention to produce a single-volume book covering all aspects and approaches to the problem of specific inhibitors of respiratory viruses. However, as the work progressed it became obvious that certain chapters, because of the research interests of the authors, concentrated particularly on influenza viruses. It seemed logical therefore, to divide the book into two volumes, the first emphasizing influenza and the second concentrating on other viruses as well as discussing important general aspects of drug screening and clinical testing, although the second volume does have some chapters which deal mainly with influenza.

* Presents a comprehensive, systematic evaluation of the various putative mediators of septic shock through the use of meta-analysis* Provides an integrated perspective that describes how these many mediators interact* Covers how advances in mathematical modeling of complex realities are applied to the field of septic shock pathogenesis

In one convenient source, this ready reference brings together for the first time, all the information available on various cyanobacterial symbioses/symbiotic cyanobacteria. Comprehensive data on structure, physiology, biochemistry and molecular biology of the cyanobiont in various cyanobacterial symbioses is included. Aplied aspects such as use of Azolla in rice cultivation and artificial symbioses are addressed, along with a chapter dedicated to methodology. This informative new text is useful to researchers, teachers, and students.

The principle objective of this book is to review the biological characteristics of estuaries. The volume has been as a text for undergraduates and graduate students as well as reference for scientists conducting research on estuarine systems. And the rapid development of estuarine ecology as a field of scientific inquiry reflects a growing awareness of the immense societal importance of a coastal ecosystem. While the volume of literature on estuaries amassed, scientists deemed it necessary to synthesize the field periodically. Consiquently, several books have been produced in recent years which examine variuous aspects of the disicpline.

Food Biopreservatives of Microbial Origin provides basic and applied information regarding how antimicrobial metabolites of safe, food-grade bacteria (used in food fermentation) can be utilized as food preservatives. The authors discuss why biopreservation of food is important, identify the foods and microoganisms for which biopreservation is suitable, and explore the potential of bacteriocins of food-grade starter culture bacteria and the antimicrobial proteins of yeasts as possible food biopreservatives. The book is a valuable reference resource that will benefit students of food science and researchers in food industries, regulatory agencies, and advisory groups.

This book places greater emphasis on the practical problems of epidemiology, prevention, diagnosis, and pathological aspects of the two principle types of free-living amoebic infection: the acute disease produced by Naegleria fowleri and the chronic, subacute and opportunistic form, produced by acanthamoeba spp.

This book discusses the topic of the HLA antigen system. Chapters discuss topics such as; animal studies, the possible mechanisms of HLA and disease associations, statistical and genetic considerations, the clinical use of HLA typing, as well as the current status of HLA and disease associations.

Legionellosis is a text in two volumes that presents the modern viewpoint of the agent and the disease. It also chronicles the history of the discovery of Legionella pneumophila. Volume 1 discusses current aspects of the microbe including taxonomy, morphology, biochemistry, and physiology. It also discusses the illness including clinical features, pathology, and therapy. Volume II details the laboratory diagnosis, epidemiology, and pathology. The contributors are amongst the most eminent scientists in their respective fields.