Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms. Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field. Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Matrix Metalloproteinase Protocols, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume.

A primary component of cell signaling research, this title covers the principal membrane-bound receptor families, including their structural organization. Written and edited by experts in the field, this book provides up-to-date research on transmembrane signaling entities and their initiating responses following extracellular stimulation.

Articles written and edited by experts in the field
Thematic volume covering effectors, cytosolic events, nuclear, and cytoplasmic events
Up-to-date research on signaling systems and mutations in transcription factors that provide new targets for treating disease

The novel coronavirus disease 2019 (COVID-19) pandemic has posed a major threat to human life and health. This book is beneficial for interdisciplinary students, researchers, and professionals to understand COVID-19 and how computational intelligence can be used for the purpose of surveillance, control, prevention, prediction, diagnosis, and potential treatment of the disease. The book contains different aspects of COVID-19 that includes fundamental knowledge, epidemic forecast models, surveillance and tracking systems, IoT- and IoMT-based integrated systems for COVID-19, social network analysis systems for COVID-19, radiological images (CT, X-ray) based diagnosis system, and computational intelligence and in silico drug design and drug repurposing methods against COVID-19 patients. The contributing authors of this volume are experts in their fields and they are from various reputed universities and institutions across the world. This volume is a valuable and comprehensive resource for computer and data scientists, epidemiologists, radiologists, doctors, clinicians, pharmaceutical professionals, along with graduate and research students of interdisciplinary and multidisciplinary sciences.

The book is based on lectures presented on the International Summer School on Biophysics held in Croatia in September 2009. The advantage of the School is that it provides advanced training in very broad scope of areas related to biophysics contrary to other similar schools or workshops that are centered mainly on one topic or technique. In this volume, tenth in the row, the papers in the field of biophysics are presented. The topics are biological phenomena from single protein to macromolecular aggregations structure by using variant physical methods (NMR, EPR, FTIR, Mass Spectrometry, etc.). The interrelationship of supramolecular structures and their functions is enlightened by applications of principals of these physical methods in the biophysical and molecular biology context.

This manual offers a stand-alone reading companion, unique in simplifying the practical components of Bioinformatics in a unique and user-friendly manner. It covers the practical component of syllabi used at most leading universities and discusses the most extensively used tools and methodologies in Bioinformatics. Research in the biological sciences has made tremendous strides in recent years due in part to the increased automation in data generation. At the same time, storing, managing and interpreting huge volumes of data has become one of the most challenging tasks for scientists. These two aspects have ultimately necessitated the application of computers, giving rise to a highly interdisciplinary discipline-Bioinformatics. Despite the richness of bioinformatics resources and methods, the exposure of life sciences undergraduates and postgraduates to bioinformatics is extremely limited. Though the internet offers various tools for free, and provides guides for using them, it fails to help users interpret the processed data. Moreover, most sites fail to update their help pages to accommodate software upgrades. Though the market is flooded with books discussing the theoretical concepts in Bioinformatics, a manual of this kind is rarely found. The content developed to meet the needs of readers from diverse background and to incorporate the syllabi of undergraduate and postgraduate courses at various universities.

This book sheds new light on the physiology, molecular biology and pathophysiology of epithelial ion channels and transporters. It combines the basic cellular models and functions by means of a compelling clinical perspective, addressing aspects from the laboratory bench to the bedside. The individual chapters, written by leading scientists and clinicians, explore specific ion channels and transporters located in the epithelial tissues of the kidney, intestine, pancreas and respiratory tract, all of which play a crucial part in maintaining homeostasis. Further topics include the fundamentals of epithelial transport; mathematical modeling of ion transport; cell volume regulation; membrane protein folding and trafficking; transepithelial transport functions; and lastly, a discussion of transport proteins as potential pharmacological targets with a focus on the pharmacology of potassium channels.

Biological interactions of visible light with photosensitizers have been studied for over a century while controlled clinical applications of light and photosensitizers to treat solid tumors, known as photodynamic therapy, have been evolving since the mid 1970's. In Photodynamic Therapy: Methods and Protocols, leading PDT scientists and clinicians provide the first comprehensive collection of methods and protocols specifically related to relevant mechanistic, dosimetric, preclinical, and clinical procedures used in current PDT research. Reflecting the growing number of studies demonstrating that immunological, tumor microenvironmental, and vascular responses are all contributing to PDT treatment outcomes, the contents of this volume move beyond the more traditional PDT concepts in order to investigate the numerous signal transduction and cell death pathways involved. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Photodynamic Therapy: Methods and Protocols is an ideal guide for new investigators just starting out in PDT research as well as seasoned investigators changing the direction of their research with the intention of exploring this vital field of study."

Advances in molecular biology over the last several decades are being steadily applied to our understanding of the molecular biology of cancer, and these advances in knowledge are being translated into the clinical practice of oncology. This volume explores some of the most exciting recent advances in basic research on the molecular biology of cancer and how this knowledge is leading to advances in the diagnosis, treatment, and prevention of cancer.

* This series provides a forum for discussion of new discoveries, approaches, and ideas * Contributions from leading scholars and industry experts * Reference guide for researchers involved in molecular biology and related fields

Understanding how digestive organs develop, their physiology and structure is important for determining new therapies to combat diseases of the digestive organs. This volume reviews the latest research and developments in this field.

* Discusses new discoveries, approaches, and ideas * Contributions from leading scholars and industry experts * Reference guide for researchers involved in molecular biology and related fields

Over the last two decades advances in genotyping technology, and the development of quantitative genetic analytical techniques have made it possible to dissect complex traits and link quantitative variation in traits to allelic variation on chromosomes or quantitative trait loci (QTLs). In Quantitative Trait Loci (QTLs):Methods and Protocols, expert researchers in the field detail methods and techniques that focus on specific components of the entire process of quantitative train loci experiments. These include methods and techniques for the mapping populations, identifying quantitative trait loci, extending the power of quantitative trait locus analysis, and case studies. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Quantitative Trait Loci (QTLs):Methods and Protocols aids scientists in the further study of the links between phenotypic and genotypic variation in fields from medicine to agriculture, from molecular biology to evolution to ecology.

"International Review of Cell and Molecular Biology "presents current advances and comprehensive reviews in cell biology--both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Impact factor for 2008: 4.935.

* Authored by some of the foremost scientists in the field * Provides up-to-date information and directions for future research * Valuable reference material for advanced undergraduates, graduate students and professional scientists

"International Review of Cell and Molecular Biology "presents current advances and comprehensive reviews in cell biology--both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Impact factor for 2008: 4.935.

* Authored by some of the foremost scientists in the field * Provides up-to-date information and directions for future research * Valuable reference material for advanced undergraduates, graduate students and professional scientists

"International Review of Cell and Molecular Biology "presents current advances and comprehensive reviews in cell biology--both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Impact factor for 2008: 4.935.

* Authored by some of the foremost scientists in the field * Provides up-to-date information and directions for future research * Valuable reference material for advanced undergraduates, graduate students and professional scientists

Glycosaminoglycans (GAGs) are a family of linear polysaccharides that arefound in all animal tissues. Several are used as biomaterials, including heparin, heparin sulfate, keratan sulfate, dermatan sulfate, and chondroitin sulfate.

This volume discusses the role of GAGs in development, health and disease.

* This series provides a forum for discussion of new discoveries, approaches, and ideas * Contributions from leading scholars and industry experts * Reference guide for researchers involved in molecular biology and related fields"

Understanding organogenesis is central to current efforts to direct stem cell differentiation and function in the context of a complex organ. Defects in organogenesis are often the major manifestations of human genetic disease syndromes. This volume describes the development of 10 major organ systems, reviewed by experts in each, to provide an up-to-date overview for researchers within and outside the field, students, teachers and clinicians.

Summarizes the key morphological and cellular events

Emphasizes up-to-date research relating to molecular control mechanisms

Draws on a range of model vertebrates and contemporary experimental approaches, including lessons learnt from human developmental disease

Distils common themes and differences for comparative studies

Highlights key remaining questions and sets the agenda for future research

Guide to Techniques in Mouse Development, Part B, is an authoritative guide to different methods used in enzymology, focusing on investigating mouse development using technological advances. The text provides information regarding the principles of the methods in mouse development, and it offers readers reliable experimental protocols and recipes described comprehensively by leaders in the field of enzymology. The text is divided into three sections and organized into 25 chapters. Below are several concepts covered by the text: Lentivirus transgenesis o Germline modification using mouse stem cells Electroporation Applications of transposons in mouse genetics Functional genomics using transposon systems The use of DNA transposons in detecting cancer genes in mice Recombination, conditional mutagenesis and induction of tamoxifen Genetic fate mapping using recombinases Genetic screens mouse ES cells Gene trap mutagenesis Mouse mutagenesis Self- renewal and pluripotency Transgenic RNAi applications Gene knockdowns Tetracycline-controlled transcription Gene expression profiling of mouse embryos The book is a comprehensive guide for students and professionals in genetics, cytology and molecular biology, who will find this book invaluable for their learning and practice.

This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development.

This volume is the first of a 2 part update of volume 225, "Guide to Techniques in Mouse Development," edited by P.M. Wassarman and M.L. DePamphilis and published in 1993.

Comprehensively covers new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap mutagenesis, analysis of allele-specific expression, use of new reporter constructs, humanizing of transgenic animals, transcript profiling of mouse development, imaging of mouse development, and rederivation of animals and use of mouse genomics.

In Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings.

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. This volume captures a snapshot of this vibrant, rapidly expanding field, presenting articlesfrom pioneers in the fieldintended to guide both the newcomer and the expert through the intricacies of getting single molecule tools.

* Includestime-tested coremethods and new innovationsapplicable to any researcher employing single molecule tools * Methods included are useful to both establishedresearchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines"

Signal transduction comprises the intracellular biochemical signals which induce the appropriate cell response to an external stimulus. The players in signal transduction are diverse, from small molecules as first messengers, to proteins, receptors, transcription factors, among many others. The different signaling pathways and the crosstalk between them originates the unique signaling profile of every cell type in the human body. The cell signaling specificity depends on several aspects including protein composition, subcellular localization and complexes and gene promoters. This textbook provides a comprehensive overview of the specific signaling pathways on a variety of human tissues. This information can be of great value for health science researchers, professionals and students to understand key pathways for tissue-specific functions in the plethora of signals, signals receptors, transducers and effectors. Chapter 3 and 15 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

One of the most pressing tasks in biotechnology today is to unlock the function of each of the thousands of new genes identified every day. Scientists do this by analyzing and interpreting proteins, which are considered the task force of a gene. This single source reference covers all aspects of proteins, explaining fundamentals, synthesizing the latest literature, and demonstrating the most important bioinformatics tools available today for protein analysis, interpretation and prediction. Students and researchers of biotechnology, bioinformatics, proteomics, protein engineering, biophysics, computational biology, molecular modeling, and drug design will find this a ready reference for staying current and productive in this fast evolving interdisciplinary field.

Explains all aspects of proteins including sequence and structure analysis, prediction of protein structures, protein folding, protein stability, and protein interactions

Teaches readers how to analyze their own datasets using available online databases, software tools, and web servers, which are listed and updated on the book s web companion page.

Presents a cohesive and accessible overview of the field, using illustrations to explain key concepts and detailed exercises for students."

"International Review of Cell and Molecular Biology "presents current advances and comprehensive reviews in cell biology--both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Impact factor for 2008: 4.935.

* Authored by some of the foremost scientists in the field * Provides up-to-date information and directions for future research * Valuable reference material for advanced undergraduates, graduate students and professional scientists

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. This volume captures a snapshot of this vibrant, rapidly expanding field, presenting articlesfrom pioneers in the fieldintended to guide both the newcomer and the expert through the intricacies of getting single molecule tools.

* Includestime-tested coremethods and new innovationsapplicable to any researcher employing single molecule tools * Methods included are useful to both establishedresearchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines"

The book introduces the bioinformatics tools, databases and strategies for the translational research, focuses on the biomarker discovery based on integrative data analysis and systems biological network reconstruction. With the coming of personal genomics era, the biomedical data will be accumulated fast and then it will become reality for the personalized and accurate diagnosis, prognosis and treatment of complex diseases. The book covers both state of the art of bioinformatics methodologies and the examples for the identification of simple or network biomarkers. In addition, bioinformatics software tools and scripts are provided to the practical application in the study of complex diseases. The present state, the future challenges and perspectives were discussed. The book is written for biologists, biomedical informatics scientists and clinicians, etc. Dr. Bairong Shen is Professor and Director of Center for Systems Biology, Soochow University; he is also Director of Taicang Center for Translational Bioinformatics.

This volume reviews the most important recent findings of the studies on pathogenic E. coli providing a timely overview of the field. The topics covered include epidemiology of the disease in humans and animals and the biological mechanisms that shaped the pathogenic types of E. coli; shiga toxins; subtilase cytotoxin; cell cycle modulating toxins; the heat stable and heat labile exterotoxins; and much more.