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Books > Science & Mathematics > Biology, life sciences > Molecular biology
This volume on metabonomics provides detailed information on the procedures involved in nuclear magnetic resonance (NMR) spectroscopy, gas chromatography-mass spectrometry (GS-MS), liquid chromatography-mass spectrometry (LC-MS), and capillary electrophoresis-mass spectrometry (CE-MS). Chapters focus on technologies and chemometrics, generation of metabonomics data, extraction of meaningful information from data, drug development, toxicology, diagnostics, and describing metabonomics as an essential part of systems biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
This volume of "Progress in Molecular Biology and Translational
Science" covers the recent advances in the expanding fields of
nutrigenetics and nutrigenomics. Forty authors from eight countries
have contributed to the publication, representing the most
cutting-edge research available. Key features: * Contributions from leading authorities * Informs and updates on all the latest developments in the field
Microsatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems. Microsatellites: Methods and Protocols brings together experts in the field to cover this significant area of research. Broken in to four convenient parts, this volume delves into classical and modern methods for the discovery and development of microsatellite markers, descriptions of amplification and visualization of SSRs, automated capillary sequencers that are widely used for fragment analysis, as well as a variety of methods for the analysis of data obtained by the use of microsatellites. Written for the highly successful Methods in Molecular Biology (TM) series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and invaluable, Microsatellites: Methods and Protocols aims at researchers that need detailed protocols for incorporating microsatellite markers into their projects and expert scientists looking to expand their knowledge of SSRs discovery, use, and analysis.
"International Review of Celland Molecular Biology "presents
current advances and comprehensive reviews in cell biology--both
plant and animal. Articles address structure and control of gene
expression, nucleocytoplasmic interactions, control of cell
development and differentiation, and cell transformation and
growth. * Authored by some of the foremost scientists in the field * Provides up-to-date information and directions for future research * Valuable reference material for advanced undergraduates, graduate students and professional scientists"
Since the identification of the first matrix metalloprotease (insterstitial collagenase or MMP-1) more than 20 closely related and evolutionarily conserved vertebrate MMPs have been discovered. Chapters in this volume detail methods and protocols on recent advances in the expression and purification of MMPs, biophysical methods such as X-ray crystallography, NMR spectroscopy and small angle X-ray scattering, computational and experimental methods for determining MMP specificity, methods for detecting MMP activity in vitro and in vivo, and methods for the development and characterization of MMP-based inhibitors as potential therapeutics for the treatment of various diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Matrix Metalloproteases: Methods and Protocols aims to ensure successful results in the further study of this vital field.
In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. Lipid Signaling Protocols, Second Edition guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the technical considerations, challenges and potential pitfalls associated with using these laboratory-based approaches. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lipid Signaling Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.
Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply. Phytoplasmas can cause serious and devastating problems for crop plants, especially in developing countries. In Phytoplasmas: Methods and Protocols experts in the field cover topics pertinent to scientists intending to develop a research program on phytoplasmas. The volume opens with an introductory chapter on the importance of phytoplasma diseases, followed by methods for detection and diagnosis as well as techniques for separating and classifying the phytoplasmas into their different taxonomic groups and subgroups. The final chapters cover methods for separating phytoplasma genomic and plasmid DNA from plant DNA for whole genome sequencing. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Phytoplasmas: Methods and Protocols serves as an ideal guide to plant pathologists and molecular biologists aiming to set up a diagnostics facility for identifying the presence of these pathogens in plants or their insect vectors.
This volume explores some of the most exciting recent advances in basic research on animal models of molecular pathology.
An edited volume describing the latest developments in approaching the problem of polymer sequence analysis, with special emphasis on the most relevant biopolymers (peptides and DNA) but not limited to them. The chapters will include peptide sequence analysis, DNA sequence analysis, analysis of biopolymers and nonpolymers, sequence alignment problems, and more.
This volume explores some of the most exciting recent advances in
basic research on molecular assembly in natural and engineered
systems and how this knowledge is leading to advances in the
various fields.
This book contains research contributions from leading global scholars in nature-inspired computing. It includes comprehensive coverage of each respective topic, while also highlighting recent and future trends. The contributions provides readers with a snapshot of the state of the art in the field of nature-inspired computing and its application. This book has focus on the current researches while highlighting the empirical results along with theoretical concepts to provide a comprehensive reference for students, researchers, scholars, professionals and practitioners in the field of Advanced Artificial Intelligence, Nature-Inspired Algorithms and Soft Computing.
This book: (i) introduces fundamental and applied bioinformatics research in the field of plant life sciences; (ii) enlightens the potential users towards the recent advances in the development and application of novel computational methods available for the analysis and integration of plant -omics data; (iii) highlights relevant databases, softwares, tools and web resources developed till date to make ease of access for researchers working to decipher plant responses towards stresses; and (iv) presents a critical cross-talks on the available high-throughput data in plant research. Therefore, in addition to being a reference for the professional researchers, it is also of great interest to students and their professors. Considering immense significance of plants for all lives on Earth, the major focus of research in plant biology has been to: (a) select plants that best fit the purposes of human, (b) develop crop plants superior in quality, quantity and farming practices when compared to natural (wild) plants, and (c) explore strategies to help plants to adapt biotic and abiotic/environmental stress factors. Accordingly the development of novel techniques and their applications have increased significantly in recent years. In particular, large amount of biological data have emerged from multi-omics approaches aimed at addressing numerous aspects of the plant systems under biotic or abiotic stresses. However, even though the field is evolving at a rapid pace, information on the cross-talks and/or critical digestion of research outcomes in the context of plant bioinformatics is scarce. "Plant Bioinformatics: Decoding the Phyta" is aimed to bridge this gap.
The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form. In "Molecular Chaperones: Methods and Protocols," expert researchers address a wide variety of approaches to the study these mechanisms, featuring the workings of heat shock proteins and heat shock transcription factors, "in vitro" and "in vivo." Written in the highly successful "Methods in Molecular Biology " series format, chapters features introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, "Molecular Chaperones: Methods and Protocols" serves as an ideal guide for all scientists who wish to pursue this vital biological action and its impact on human health and disease."
Few problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent reduction of atmospheric dinitrogen to bioavailable ammonia. In "Nitrogen Fixation: Methods and Protocols," recognized experts in the field provide an up-to-date, in-depth overview of the methods that have been applied to studying the nitrogenase at a molecular level, ranging from genetic, biochemical, spectroscopic, and chemical methods to theoretical calculations. In addition, techniques used to study an enzyme system that is homologous to nitrogenase are described in this book. Written in the highly successful "Methods in Molecular Biology " series format, methods chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, "Nitrogen Fixation: Methods and Protocols" will be useful for anyone interested in nitrogenase research and willing to venture further toward addressing the remaining mechanistic and biosynthetic questions of this fascinating enzyme system."
Cell death is an essential process in development, and a major contributor to a wide range of human diseases. Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Necrosis: Methods and Protocols describes techniques in an easy to follow manner, with details so that beginners can succeed with challenging techniques.
Structural genomics is the systematic determination of 3D
structures of proteins representative of the range of protein
structure and function found in nature. The goal is to build a body
of structural information that will predict the structure and
potential function for almost any protein from knowledge of its
coding sequence. This is essential information for understanding
the functioning of the human proteome, the ensemble of tens of
thousands of proteins specified by the human genome.
Proteins are the most diverse group of biologically important substances. With the recent technological advances in the genomics area and the efforts in proteomics research, the rate of discovery for new proteins with unknown structure and function has increased. These proteins generated from genomic approaches present enormous opportunities for research and industrial application. Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods is a compilation of chapters within the exciting area of protein purification designed to give the laboratory worker the information needed to design and implement a successful purification strategy. It presents reliable and robust protocols in a concise form, emphasizing the critical aspects on practical problems and questions encountered at the lab bench. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods will be an ideal source of scientific information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, and biotechnology and other related areas in both academia and industry.
Soil salinity is destroying several hectares of arable land every minute. Because remedial land management cannot completely solve the problem, salt tolerant crops or plant species able to remove excessive salt from the soil could contribute significantly to managing the salinity problem. The key to engineering crops for salt tolerance lies in a thorough understanding of the physiological mechanisms underlying the adaptive responses of plants to salinity. Plant Salt Tolerance: Methods and Protocols describes recent advances and techniques employed by researchers to understand the molecular and ionic basis of salinity tolerance and to investigate the mechanisms of salt stress perception and signalling in plants. With chapters written by leading international scientists, this book covers nearly 30 different methods, such as microelectrode and molecular methods, imaging techniques, as well as various biochemical assays. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Salt Tolerance: Methods and Protocols serves as an essential read for every student or researcher tackling various aspects of the salinity problem.
DNA methylation is essential for the normal development and
functioning of organisms. This volume discusses the latest
developments in this very active field of research. It presents the
evolution of DNA methylation, mammalian DNA methyltransferases, DNA
methylation and demethlyation, DNA methylation and silencing and
the role it plays in medicine including cancer. * Discusses new discoveries, approaches, and ideas * Contributions from leading scholars and industry experts * Reference guide for researchers involved in molecular biology and related fields
This volume focuses on various approaches to studying long non-coding RNAs (lncRNAs), including techniques for finding lncRNAs, localization, and observing their functions. The chapters in this book cover how to catalog lncRNAs in various plant species; determining subcellular localization; protein interactions; structures; and RNA modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and innovative, Plant Long Non-Coding RNAs: Methods and Protocols is a valuable resource that aids researchers in understanding the functions of lncRNAs in different plant species, and helps them explore currently uncharted facets of plant biology.
First published in 1943, "Vitamins and Hormones" is the longest-running serial published by Academic Press. The Editorial Board now reflects expertise in the field of hormone action, vitamin action, X-ray crystal structure, physiology and enzyme mechanisms. Under the capable and qualified editorial leadership of Dr. Gerald Litwack, "Vitamins and Hormones" continues to publish cutting-edge reviews of interest to endocrinologists, biochemists, nutritionists, pharmacologists, cell biologists and molecular biologists. Others interested in the structure and function of biologically active molecules like hormones and vitamins will, as always, turn to this series for comprehensive reviews by leading contributors to this and related disciplines. This volume focuses on vitamins and the immune system.
This book contains articles written by experts on a wide range of topics that are associated with the analysis and management of biological information at the molecular level. It contains chapters on RNA and protein structure analysis, DNA computing, sequence mapping, genome comparison, gene expression data mining, metabolic network modeling, and phyloinformatics. The important work of some representative researchers in bioinformatics is brought together for the first time in one volume. The topic is treated in depth and is related to, where applicable, other emerging technologies such as data mining and visualization. The goal of the book is to introduce readers to the principle techniques of bioinformatics in the hope that they will build on them to make new discoveries of their own. Contents: Exploring RNA Intermediate Conformations with the Massively Parallel Genetic Algorithm; Introduction to Self-Assembling DNA Nanostructures for Computation and Nanofabrication; Mapping Sequence to Rice FPC; Graph Theoretic Sequence Clustering Algorithms and their Applications to Genome Comparison; The Protein Information Resource for Functional Genomics and Proteomics; High-Grade Ore for Data Mining in 3D Structures; Protein Classification: A Geometric Hashing Approach; Interrelated Clustering: An Approach for Gene Expression Data Analysis; Creating Metabolic Network Models Using Text Mining and Expert Knowledge; Phyloinformatics and Tree Networks. Readership: Molecular biologists who rely on computers and mathematical scientists with interests in biology.
Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms. Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field. Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Matrix Metalloproteinase Protocols, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume. |
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