Pathophysiological states, neurological and psychiatric diseases are almost universally considered from the neurocentric point of view, with neurons being the principal cellular element of pathological process. The brain homeostasis, which lies at the fulcrum of healthy brain function, the compromise of which invariably results in dysfunction/disease, however, is entirely controlled by neuroglia. It is becoming clear that neuroglial cells are involved in various aspects of initiation, progression and resolution of neuropathology. In this book we aim to integrate the body of information that has accumulated in recent years revealing the active role of glia in such pathophysiological processes. Understanding roles of glial cells in pathology will provide new targets for medical intervention and aide the development of much needed therapeutics. This book will be particularly useful for researchers, students, physicians and psychotherapists working in the field of neurobiology, neurology and psychiatry.

There are several types of damage that can be found in the male gamete. Genetic damage in spermatozoa can originate during spermatogenesis, or it can originate during transit in both male and female genital tracts. Damage can also be due to ageing, environmental or iatrogenic conditions, as well as to the protocols to cryopreserve and to select spermatozoa in assisted reproduction techniques. The purpose of this book is to provide a comprehensive resource for all possible DNA damages in sperm, the relation to fertility and infertility, and possible transgenerational heritable effects.

Pancreatic ductal adenocarcinoma is the fifth leading cause of cancer death in the USA. Pancreatic cancer develops as a result of the accumulation of genetic and epigenetic alterations in cancer-causing genes such as oncogenes and tumor-suppressor genes. The second edition of Pancreatic Cancer: Methods and Protocols provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of sporadic and familial pancreatic cancer. It covers topics from in-vitro cell cultures to in-vivo mouse models, DNA to protein manipulation, and genetic and epigenetic analyses to treatment development. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, written by highly renowned investigators with expertise in pancreatic cancer, Pancreatic Cancer: Methods and Protocols, Second Edition is an invaluable source of proven protocols to those who are interested in joining the fight against pancreatic cancer.

This issue of Thoracic Surgery Clinics includes well-illustrated articles on surface anatomy, the pleura and pleural spaces (including the anatomy of the parietal and visceral pleura, the pleural reflections and recesses, and microscopic anatomy of the pleura), anatomy of the? mediastinum (including mediastinal divisions and compartments, the thymus, the superior vena cava and brachiocephalic veins, the heart and pericardium, the thoracic aorta and its branches, the thoracic duct, and the nerves of the thorax) and the anatomy of the diaphragm and the esophagus (including? vascular supply, lymphatic pathways, innvervation).

Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks.

A major revision of the leading textbook of FNA cytology Both text and illustrations provide a systematic and logical approach to the acquisition, interpretation and diagnosis of Fine Needle Aspirate biopsy specimens. Orell's FNA Cytology will assist in establishing diagnostic criteria and results in the examination of benign and malignant condition in all body systems where the technique is currently applied. The information is always presented in a clinical context: it details the role of FNA and other ancillary techniques in the investigative sequence (the cytological findings are always set in the context of the patient's clinical history, physical exam and other laboratory tests). It gives practical, technical advice on how to obtain good smears and alerts the reader as to possible diagnostic pitfalls and complications. The contents are organized in a framework of anatomical regions (rather than histopathological classifications) in order to reflect the way problems present in clinical practice. Each chapter is divided into two parts: the first deals with indications, accuracy, techniques and complications; the second illustrates the cytological patterns and their implication. Both common and rare conditions are covered.Provides practical tips and advice on how to avoid pitfalls and ensure accurate diagnoses. Over 1,200 colour illustrations capture each entity's cellular, morphological and immunohistochemical appearance. Chapters have been up-dated and revised and a brand new one on cytological findings in infectious diseases added. Both MGG and Pap smears illustrated in parallel as well as the corresponding histology to help provide side-by-side analysis. Access the full text online and download images via Expert Consult. Brand new chapter on cytological findings in infectious diseases. Inclusion of immuno-profiles and other relevant ancillary tests. New illustrations. New contributing authors. Available online via Expert Consult.

This book examines in depth the evidence, clinical applications and potential cancer signatures in the circulation and discusses alterations in circulating cell-free nucleic acids, and circulating tumor DNA, as well as the epigenome, genome, transcriptome (coding and noncoding), proteome (both traditional serum proteins and proteomic profiles) and metabolome. Further, it highlights the clinical applications of circulating tumor cells for each cancer type and addresses the emerging importance of extracellular vesicular contents, including miRNA, oncogenes and drug resistant factors. As such, it offers a valuable reference guide for cancer researchers, oncologists, clinicians, surgeons, medical students, oncology nurses, diagnostic laboratories, and the pharmaceutical industry.

Reproductive medicine is a growing field with new technology emerging faster than we can assess consumer's perceptions of -the number of cancer survivors are growing and there is a great need to attend to their quality of life-this book addresses the needs of males and females, identifies effective communication strategies and proactive measures for health care professionals and researchers to use as well as identifying gaps in the literature where more research is needed.

The book "Paradigm Shift for Future Tennis" starts with revelations that make obvious the limitations of today's tennis, which does not use the laws of modern Biomechanics and Neurophysiology. The second part of the book includes a new approach to the quantum mind of a champion. It will reveal the secret weapon of Roger Federer and the blueprint of a future tennis champion. This book will expose the new tennis shot emerging from the field of sports science. It is a real weapon, which can generate a ball-speed similar to that of the first serve: the Power High-Forehand. Its aim is to generate maximal possible racket-head speed while players do not wait for the ball to bounce. This is both a tactical and psychological basis for the future tennis game. This aggressive interceptive psychology will shape the minds of future tennis champions. High racket-head speed can be achieved using the stretch-reflex, without big loops and swings. Weapons of a future tennis game will comprise of whip-like tennis serves and ground strokes, based on the stretch-reflex, and using the whole body in a fluid and integrated manner, thus manifesting a superb combination of speed and strength. Restructure your brain and apply the power of state of the art biomechanical, mathematical, medical, neural, cognitive, and quantum computational intelligence to understand the tennis of today and the future

Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. Thisnew edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay.
* Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays
* Discusses many new assays developed since the previous edition
* Presents recent developments in cytometric intrumentation/technology"

Calcium ions represent Mother Nature's 'ion-of-choice' for regulating fundamental physiological functions, as they initiate a new life at the time of fertilization and guide subsequent developmental and physiological functions of the human body. Calcium channels, which act as gated pathways for the movement of calcium ions across the membranes, play a central part in the initiation of calcium signals, and defects in calcium channel function have been found to result in a plethora of human diseases, referred to as the calcium channelopathies. Pathologies of Calcium Channels brings together leading international experts to discuss our current understanding of human diseases associated with the various calcium channels, from their molecular basis to potential future therapeutic targeting of calcium channels.

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.

'A stunner ... If you haven't got this book in your house, I don't know why' Chris Evans 'A startling wake-up call . . . Writing with the vim of a Bill Bryson and the technical knowledge of a scientist, Steele gives us a chance to grasp what's at stake' Independent 'An exhilarating journey . . . Steele is a superb guide' Telegraph 'A fascinating read with almost every page bursting with extraordinary facts . . . Read it now' Mail on Sunday Ageless is a guide to the biggest issue we all face. Ageing - not cancer, not heart disease - is the world's leading cause of death and suffering. What would the world be like if we could cure it? Living disease-free until the age of 100 is achievable within our lifetimes. In prose that is lucid and full of fascinating facts, Ageless introduces us to the cutting-edge research that is paving the way for this revolution. Computational biologist Andrew Steele explains what occurs biologically as we age, as well as practical ways we can slow down the process. He reveals how understanding the scientific implications of ageing could lead to the greatest discovery in the history of civilisation - one that has the potential to improve billions of lives, save trillions of dollars, and transform the human condition.

This book sheds new light on the development and use of quantitative models to describe the process of skin permeation. It critically reviews the development of quantitative predictive models of skin absorption and discusses key recommendations for model development. Topics presented include an introduction to skin physiology; the underlying theories of skin absorption; the physical laboratory-based processes used to generate skin absorption data, which is in turn used to construct mathematical models describing the skin permeation process; algorithms of skin permeability including quantitative structure-activity (or permeability) relationships (QSARs or QSPRs); relationships between permeability and molecular properties; the development of formulation-focused approaches to models of skin permeability prediction; the use of artificial membranes, e.g. polydimethylsiloxane as alternatives to mammalian skin; and lastly, the use of novel Machine Learning methods in developing the next generation of predictive skin permeability models. The book will be of interest to all researchers in academia and industry working in pharmaceutical discovery and development, as well as readers from the field of occupational exposure and risk assessment, especially those whose work involves agrochemicals, bulk chemicals and cosmetics.

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.

Medical research involving human subjects has contributed to considerable advancements in our knowledge, and to medical benefits. At the same time the development of new technologies as well as further globalisation of medical research raises questions that require the attention of researchers from a range of disciplines. This book gathers the contributions of researchers from nine different countries, who analyse recent developments in medical research from ethical, historical, legal and socio-cultural perspectives. In addition to reflections on innovations in science such as genetic databases and the concept of "targeted therapy" the book also includes analyses regarding the ethico-legal regulation of new technologies such as human tissue banking or the handling of genetic information potentially relevant for participants in medical research. Country and culture-specific aspects that are relevant to human medical research from a global perspective also play a part. The value of multi- and interdisciplinary analysis that includes the perspectives of scholars from normative and empirical disciplines is a shared premise of each contribution.

Today, individuals have greater access to information about their healththaneverbefore(Randeree,2009;Eysenbach,2008).Muchofthis changeisdue, inlargepart, toadvancesinbiotechnologyandtheseque- ing of the human genome (Manolio & Collins, 2009). It is now possible, forexample, forindividualstologontotheInternetand, forafeeofs- eral hundred dollars, order an at-home DNA collection kit and have the resultsofamyriadofgenetictestsdelivereddirectlytotheire-mailinbox (Gurwitz&Bregman-Eschet,2009).Insomecases, thesetestresultsmay indicatepersonalriskforcommonchronicdiseases, suchascertainforms ofcancer, diabetes, cardiovasculardisease, andseveralothers.Companies marketing these test kits often claim that promoting greater access to and awareness of the association between genes and health, and one's genetic susceptibilities to disease, leads to more proactive and insig- fulmethodsofindividualhealthmanagement(Hogarth, Javitt,&Melzer, 2008). Moreover, it is consistent with an emerging trend in medicine - that of consumer-oriented medicine - which places health information toolsdirectlyinthehandsofpatientsunderthepremiseoffosteringbetter patient-providercollaboration(Silvestre, Sue,&Allen,2009). Though the principles behind this direct-to-consumer approach to genetics seem laudable and perhaps even exciting, there is consid- ablecontroversyastowhat, ifany, utilitytheinformationactuallyholds (Geransar&Einsiedel,2008;Wasson, Cook,&Helzlsouer,2006).Unlike geneticteststhatarediagnostic(e.g., chromosomeanalysisforDowns- drome)orhighlypredictive(e.g., BRCA1andBRCA2testingforhereditary breast-ovarian cancer risk), this new wave of presymptomatic predictive genetictestsforcommondiseaseyieldsresultsthataremuchmoreunc- tainbecausethestatisticalmodelsonwhichtheyarepresentlybasedare imperfectandwithlimiteddata(Ng, Murray, Levy,&Venter,2009). Theabovescenarioraisesmanyquestionsfortoday'shealth-carec- sumers. For example, for whom is this information applicable, and for whatpopulationsorsubpopulationsisitnot?Underwhatcircumstances might this information be useful, and when should it be disregarded as irrelevant?Andperhapsmostimportantly, what, ifanything, canbedone inlightofinformationaboutpersonalgeneticrisktoeffectivelylowerthe oddsofbecomingsickandraisetheoddsofstayinghealthy? vii viii PREFACE Becausetheprevalenceofmostdiseasesvariesasafunctionofage, gender, race/ethnicity, and other personal characteristics, answers to these questions are complex and many are just beginning to be und- stood(Khouryetal.,2009).Someexpertshaveconcludedthattheanswers tosuchquestionsremainoutofreachatthepresenttimeandmayc- tinue to be elusive for another 5-10 years (Frazer, Murray, Schork, & Topol,2009).Yet, twenty-?rstcenturyhealth-careconsumers, providers, and policy makers face these choices now about incorporating personal genetic information into health management and often do so without a complete and accurate understanding of the potential impact of their decisionsonmultiplelevels(Carlson,2009).

Androgens and androgen receptors (AR) play critical roles in the development and progression of prostate cancer, the most frequently diagnosed cancer and second leading cause of cancer death in US males. AR is an androgen-dependent DNA-binding transcription factor that regulates the expression of androgen-responsive genes. Identification and characterization of androgen-responsive genes provide insights into the cellular mechanisms of androgen action and may lead to new approaches in diagnosis, prognosis, prevention and/or treatment of prostate cancer. This volume provides critical information from well respected experts in the field. Some of the exciting topics include the new understanding of mechanisms underlining the regulation of androgen-responsive gene expression, and functions of various androgen-responsive genes in biological processes essential in carcinogenesis including cell growth, angiogenesis, and epithelial-to-mesenchyme transition (EMT). Other important aspects addressed are the current and potential clinic applications of knowledge on androgen-responsive gene regulation and function. This book is intended for researchers, scientists, faculty, and advanced graduate students with an interest in androgen action and prostate cancer.

This book presents a comprehensive collection of detailed state-of-the-art exon skipping and splices modulation protocols. Chapters detail 14 genetic diseases, AON-mediated therapies, and CRISPR/Cas9-mediated gene editing therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Exon Skipping and Inclusion Therapies: Methods and Protocols aims to help researchers initiate the development of next-generation therapies.

This book sheds new light on the physiology, molecular biology and pathophysiology of epithelial ion channels and transporters. It combines the basic cellular models and functions by means of a compelling clinical perspective, addressing aspects from the laboratory bench to the bedside. The individual chapters, written by leading scientists and clinicians, explore specific ion channels and transporters located in the epithelial tissues of the kidney, intestine, pancreas and respiratory tract, all of which play a crucial part in maintaining homeostasis. Further topics include the fundamentals of epithelial transport; mathematical modeling of ion transport; cell volume regulation; membrane protein folding and trafficking; transepithelial transport functions; and lastly, a discussion of transport proteins as potential pharmacological targets with a focus on the pharmacology of potassium channels.

First published in 1943, "Vitamins and Hormones" is the longest-running serial published by Academic Press. The Editorial Board now reflects expertise in the field of hormone action, vitamin action, X-ray crystal structure, physiology, and enzyme mechanisms.
Under the capable and qualified editorial leadership of Dr. Gerald Litwack, "Vitamins and Hormones" continues to publish cutting-edge reviews of interest to endocrinologists, biochemists, nutritionists, pharmacologists, cell biologists, and molecular biologists. Others interested in the structure and function of biologically active molecules like hormones and vitamins will, as always, turn to this series for comprehensive reviews by leading contributors to this and related disciplines.
This volume focuses on insulin and IGFs.
*Longest running series published by Academic Press
*Contributions by leading international authorities

Significant advances in our knowledge of genetics were made during the twentieth century but in the most recent decades, genetic research has dramatically increased its impact throughout society. Genetic issues are now playing a large role in health and public policy, and new knowledge in this field will continue to have significant implications for individuals and society. Written for the non-majors human genetics course, Human Genetics, 3E will increase the genetics knowledge of students who are learning about human genetics for the first time. This thorough revision of the best-selling Human Genome,2E includes entirely new chapters on forensics, stem cell biology, bioinformatics, and societal/ethical issues associated with the field. New special features boxes make connections between human genetics and human health and disease. Carefully crafted pedagogy includes chapter-opening case studies that set the stage for each chapter; concept statements interspersed throughout the chapter that keep first-time students focused on key concepts; and end-of-chapter questions and critical thinking activities. This new edition will contribute to creating a genetically literate student population that understands basic biological research, understands elements of the personal and health implications of genetics, and participates effectively in public policy issues involving genetic information .
Includes topical material on forensics, disease studies, and the human genome project to engage non-specialist students

Full, 4-color illustration program enhances and reinforces key concepts and themes

Uniform organization of chapters includes interest boxes that focus on human health and disease, chapter-opening case studies, and concept statements to engage non-specialist readers