An overview of all the available literature on the various aspects of the regulation of the cardiovascular system`s function and physiology by the adrenergic neurohormonal system, i.e. the catecholamines norepinephrine and epinephrine. Although there are several books describing the adrenergic system`s biology, physiology and pharmacology, and also several excellent books on cardiovascular physiology and pathology, this book focuses exclusively on the interface of these two areas: cardiovascular regulation by the adrenergic system and how it affects cardiovascular diseases and their treatments. Each chapter describe the roles of the adrenergic system first in each cardiovascular cell type (cell type-by-cell type) and then in specific areas of cardiovascular physiology, such as in exercise and in cardiovascular metabolism. Finally, the book concludes with a chapter on the adrenergic system`s role in the currently very "hot" (in terms of scientific investigations) area of cardiovascular stem cell biology. The book covers the adrenergic system-specifically and exclusively in the heart and vessels. It is formatted by cardiovascular cell type-by-cell type manner, rather than in an organ-by-organ or in a disease-by-disease manner, as usually discussed in standard, conventional biomedical textbooks. The book also discusses the adrenergic system in novel, cutting-edge cardiovascular research areas, in which it has not been covered well so far (e.g. stem cells, exercise). These three areas constitute the most important assets of the book, which sets it apart from others in the field.

Current knowledge of the mechanisms that regulate DNA repair has grown significantly over the past years with technology advances such as RNA interference, advanced proteomics and microscopy as well as high throughput screens. The third edition of DNA Repair Protocols covers various aspects of the eukaryotic response to genomic insult including recent advanced protocols as well as standard techniques used in the field of DNA repair. Both mammalian and non-mammalian model organisms are covered in the book, and many of the techniques can be applied with only minor modifications to other systems than the one described. Written in the highly successful Methods in Molecular Biology? series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, DNA Repair Protocols, Third Edition provides expert guidance for DNA repair, recombination, and replication.

In Male Mid-Life Crisis, Justin K. Lim investigates this phase of human life through the perspectives of analytical psychology and through existential theology. Research suggests 80 percent of men suffer from this crisis, and that it is not limited to any culture or period of history. He applies his findings to pastoral/spiritual interventions, seeking deeper and broader psychological and theological insights.

For decades, "Emeryand Rimoin s Principles and Practice of Medical Genetics "has provided the ultimate source for practicing clinicians to learn how the study of genetics can be integrated into practice.Developed in parallel to the sixth edition, and featuring 174 original contributions from the many authors of the full set, this one volume work expertly condenses and synthesizes the most clinically relevant content, for convenient desk reference.

Helping to bridge the gap between high-level molecular genetics and individual application, it follows the multi-volume set inencompassing scientific fundamentals, full spectrum discussion of major inherited disorders, and actionable therapies. Clinically oriented information is supported byconcisedescriptions of theprinciples of genetics, research approaches, and analytics to embrace the evolving population of students, researchers, and practitioners who are integrating their work to provide advanced diagnosis, prevention and treatment of human disease.

This print volume is complemented and enhanced with online access to the complete text, online-only references, and high quality illustrations on www.expertconsult.com.
Features 174 summarized contributions concisely discussing advances in cancer genetics, genomic technologies, and molecular geneticsContainshundreds of full colour illustrations supporting users with identification, concept illustration, and method processingEnhanced with full text online access, high quality illustrations, and online-only references at www.expertconsult.com"

This second edition volume expands on the previous edition by presenting updated protocols for several of the techniques described in the first edition of Drosophila: Methods and Protocols and current methods that cover recent breakthroughs in Drosophila research. The book begins with a description of FlyBase--a database of genes and genomes--followed by the presentation of systems for versatile gene expression in the fly. The first few chapters in this book detail gene knockdown and editing, including CRISPR-Cas9 and protein knockdown. The next few chapters are devoted to methods describing live imaging of different tissues and organs, followed by chapters on how to quantify image data and how to probe tissue mechanics by laser ablation. The next two chapters provide methods for analyzing transcription followed by protocols to study growth, metabolism, ageing, and behavior in Drosophila. This volume concludes with chapters on electrophysiological recordings and methods to establish cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Drosophila: Methods and Protocols, Second Edition is a valuable source of hands-on protocols and reviews for molecular, cell, and developmental biologists using Drosophila as model systems in their work.

In this thematic volume of "Progress in Molecular Biology and Translational Science, "researchers reflect on recent developments and research surrounding G protein-coupled receptors. The chapters cover a large breadth of research, including GPCR role in stem cell function and pharmacology. Authors explore in-depth research techniques and applications of GPCR usage, covering theory, laboratory approaches, and unique qualities that make GPCRs a crucial tool in microbiological and cancer research.

Key features:

* Contributions from leading authorities * Informs and updates on all the latest developments in the field

For many years, it has been known that when rats and mice are given a reduced amount of food, their life span is increased and they remain healthy and vigorous at advanced ages.

What is the reason for this change in the usual pattern of aging? The evidence is overwhelming that the life extension results from a slowing of aging processes. And the factor responsible is the decrease in caloric intake. The obvious question: How does this factor work? A good question - and the reason that research on the anti-aging action of caloric restriction is today one of the most studied research areas in biological gerontology. For it is felt that if the biological mechanisms of the anti-aging action of caloric restriction can be uncovered, we would gain an understanding of the basic nature of aging processes, which would, in turn, yield possible interventions in human aging. This book aims to provide the growing number of researchers in this field (faculty, postdoctoral trainees, and graduate students) with a detailed knowledge of what is known about caloric restriction within the frame of gerontology, as well as insights on future of this field.

In a normal physiological state, several bacteria are present in the human gut that is essential to maintain the normal to healthy gastrointestinal function. Disturbances in this "normal flora" lead to gut inflammation and infection. This volume explores the potential of probiotics, the healthy bacteria, to manage gut-related diseases including gastrointestinal cancers, ulcerative colitis, H. pylori infections, and diarrhea; vaginosis; oral health; airway inflammation; and atopic dermatitis. The concept of designer probiotics, edible vaccines and future scope of research in the field is also presented. The animal models used for studying the benefits of probiotics in gut inflammation are described for beginners.

The adult patient diagnosed with or at risk for a neurogenetic disease has many questions and concerns for the genetic counselor, the neurologist, and other practitioners. Because of the emotional and potentially life-altering impact of these diseases on the patient and family, counseling can be especially challenging. A rare hands-on guide to the subject, Genetic Counseling for Adult Neurogenetic Disease deals with core issues that differentiate adult neurogenetic counseling from its more familiar pediatric counterpart. This innovative book with accompanying videos is designed to fill in deficits in this area typical of training programs in genetic counseling (which have pediatrics and prenatal concentrations) and neurology (which rarely cover genetic counseling). For each condition featured, chapters include a detailed overview of genetic symptoms, diagnostic criteria, and management, plus guidelines for asking, and answering, pertinent questions. The major concentration, however, is on genetic counseling issues and case histories illustrating these issues. As an added dimension, the accompanying videos depict representative issues and challenges in genetic counseling for specific diseases in addition to the basics of a neurological examination. Among the conditions discussed: Movement disorders, including Parkinson's disease. Dementias, including Alzheimer's disease. Stroke. Motor neuron diseases. Neuropathies and channelopathies. Adult muscular dystrophies. Neurocutaneous syndromes. Plus a section on neurological and neuropsychological evaluation. This is information that will stay relevant as technologies change and genetic understanding evolves. Genetic Counseling for Adult Neurogenetic Disease offers advanced clinical wisdom for genetic counselors as well as neurologists, neuropsychologists, and other referring clinicians.

This new volume of "Methods in Enzymology" continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume coversG protein coupledreceptorsandincludes chapterson such topics as G protein-coupled receptor trafficking motifs, structure-based virtual screening, and automation-friendly high throughput assays for identification of pharmacoperone drugs.
Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers G protein coupled receptorsContains chapters on such topics as G protein-coupled receptor trafficking motifs, structure-based virtual screening, and automation-friendly high-throughput assays for identifying pharmacoperone drugs"

This manual is intended to guide and facilitate human anatomical dissections. It is flexible enough for use in long as well as short courses. It can be particularly useful as a link with real anatomy when used together with computerised-anatomy programs, or where students do not dissect but merely look at atlases, prosections and models. There is an introduction for each anatomical region; and for each section to be dissected there is an overview, a dissection schedule which guides the student through a set of instructions, a summary and a list of objectives that are clinically important. The terminology used is the latest. The manual is suitable for medical and dental students. It is also of value for advanced knowledge of anatomy for surgery and in relation to the interpretation of normal anatomy in non-invasive imaging of anatomy for clinical diagnosis, surgical practice on cadaveric material, and in discussions about clinical problems.

This book presents the current state of knowledge on the origin and differentiation of cell lines involved in the development of the vertebrate male and female gonads with particular emphasis on the mouse. It also discusses the processes leading to the testis- and ovary-specific structures and functions. The individual chapters review the origin and differentiation of the somatic cells of the genital ridges; the formation and migration of primordial germ cells in mouse and man; the gonadal supporting cell lineage and mammalian sex determination; differentiation of Sertoli and granulosa cells; mesonephric cell migration into the gonads and vascularization; origin and differentiation of androgen-producing cells in the gonads; germ cell commitment to the oogenic versus spermatogenic pathway and the role of retinoic acid; ovarian folliculogenesis; control of oocyte growth and development by intercellular communication within the follicular niche; biology of the Sertoli cell in the fetal, pubertal and adult mammalian testis; mechanisms regulating spermatogonial differentiation; stem cells in mammalian gonads; the role of microRNAs in cell differentiation during gonad development; human sex development and its disorders; as well as methods for the study of gonadal development.

New developments in science and technology have resulted in shifting ethical challenges in many areas including in genomics research. This book enables those who are involved in genomics research, whether as researcher, participant or policy maker, to understand the ethical issues currently developing in this field and to participate actively in these important debates. A clear account is given of how science and technology are outstripping the capacity of previous ethical regulations to cope with current issues, together with practical illustrations of possible ways forward. Key ethical ideas are presented, drawing on the history of research regulation and on an account of the particular challenges arising in the field of genomics. The book uses a grounded, practical approach to explaining ethical concepts and issues which is geared to enhancing interdisciplinary dialogue. Its broad approach to ethical issues includes relevant considerations from social psychology and there is a particular emphasis on understanding the problems of ethical regulations and practice in the institutional and social context of research. A glossary and numerous text boxes explaining relevant terms and key ideas help to make the work an invaluable resource for both beginners and experts in the field.

This volume is a collection of miRNA detection and target identification protocols, detailing new developments in the traditional detection approaches such as northern blot, quantitative real-time PCR, array, next generation sequencing, and in situ hybridization. The chapters in MicroRNA Detection and Target Identification: Methods and Protocols guide readers through novel approaches such as nanotechnology, microfluidics, based detection methods, analysis of serum and urinary, miRNAs as biomarkers, target identification and experimental approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, MicroRNA Detection and Target Identification: Methods and Protocols aims to ensure successful results in the further study of this vital field.

This new volume of "Methods in Enzymology" continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain.
Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers G protein coupled receptorsContains chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain

Obesity is an epidemic with enormous health, economic and social burdens. Current drugs for obesity treatment are far from ideal in terms of efficacy and side effects. Reviews in this volume of Progress in Molecular Biology and Translational Science summarize current status in studies of a number of G protein-coupled receptors that were shown to be promising targets for obesity treatments. Some of these receptors also cause monogenic obesity in humans.
Subject matter: obesity is an epidemic and G protein-coupled receptors are promising drug targets, with significant potential as new anti-obesity drugs.

Chapters are written by leading experts.

Ribonucleic acid (RNA) binding proteins currently number in the thousands and defects in their function are at the heart of diseases such as cancer and neurodegeneration. RNA binding proteins have become implicated in the intricate control of surprisingly diverse biological settings, such as circadian rhythm, stem cell self-renewal, oncogenesis and germ cell development. This book surveys a range of genome-wide and systems approaches to studying RNA binding proteins, the importance of RNA binding proteins in development, cancer and circadian rhythm.

This new volume of "Current Topics in Developmental Biology" covers the area of gametogenesis, with contributions from an international board of authors. The chapters provide a comprehensive set of reviews covering such topics as germline stem cells, signaling modalities during oogenesis in mammals, and genomic imprinting as a parental effect established in mammalian germ cells.
Covers the area of gametogenesisInternational board of authorsProvides a comprehensive set of reviews covering such topics as germline stem cells, signaling modalities during oogenesis in mammals, and genomic imprinting as a parental effect established in mammalian germ cells

Microsurgery for Fertility Specialists is an invaluable resource for physicians who specialize in the surgical treatment of male and female infertility. This practical text explains the basics of microsurgery and provides detailed operative descriptions of surgical procedures such as vasectomy reversal, sperm retrieval, tubal reanastomosis, and penile surgery. An exceptional cadre of reproductive specialists shares valuable knowledge of the current state of microsurgery, its role in infertility procedures, and the latest advances in the field --including some non-infertility microsurgical techniques. The book is an essential educational guide for those with a more advanced knowledge of microsurgical practice as it includes chapters that will help them mentor residents and fellows, and inspire future generations of reproductive surgeons.

This book examines the toxicological and health implications of environmental epigenetics and provides knowledge through an interdisciplinary approach. Included in this volume are chapters outlining various environmental risk factors such as phthalates and dietary components, life states such as pregnancy and ageing, hormonal and metabolic considerations and specific disease risks such as cancer cardiovascular diseases and other non-communicable diseases. Environmental Epigenetics imparts integrative knowledge of the science of epigenetics and the issues raised in environmental epidemiology. This book is intended to serve both as a reference compendium on environmental epigenetics for scientists in academia, industry and laboratories and as a textbook for graduate level environmental health courses. Environmental Epigenetics imparts integrative knowledge of the science of epigenetics and the issues raised in environmental epidemiology. This book is intended to serve both as a reference compendium on environmental epigenetics for scientists in academia, industry and laboratories and as a textbook for graduate level environmental health courses.

Microbial relationships with all life forms can be as free living, symbiotic or pathogenic. Human beings harbor 10 times more microbial cells than their own. Bacteria are found on the skin surface, in the gut and other body parts. Bacteria causing diseases are the most worrisome. Most of the infectious diseases are caused by bacterial pathogens with an ability to form biofilm. Bacteria within the biofilm are up to 1000 times more resistant to antibiotics. This has taken a more serious turn with the evolution of multiple drug resistant bacteria. Health Departments are making efforts to reduce high mortality and morbidity in man caused by them. Bacterial Quorum sensing (QS), a cell density dependent phenomenon is responsible for a wide range of expressions such as pathogenesis, biofilm formation, competence, sporulation, nitrogen fixation, etc. Majority of these organisms that are important for medical, agriculture, aquaculture, water treatment and remediation, archaeological departments are: Aeromonas, Acinetobacter, Bacillus, Clostridia, Enterococcus, Pseudomonas, Vibrio and Yersinia spp. Biosensors and models have been developed to detect QS systems. Strategies for inhibiting QS system through natural and synthetic compounds have been presented here. The biotechnological applications of QS inhibitors (QSIs) in diverse areas have also been dealt with. Although QSIs do not affect growth and are less likely to impose selective pressure on bacteria, however, a few reports have raised doubts on the fate of QSIs. This book addresses a few questions. Will bacteria develop mechanisms to evade QSIs? Are we watching yet another defeat at the hands of bacteria? Or will we be acting intelligently and survive the onslaughts of this Never Ending battle?

Parameters such as membrane transport, metabolism and protein incorporation govern the fate of amino acids in living tissue. Is it possible to use positron tomography to measure some of them, and what is their meaning in normal and pathological situations? These questions have been addressed for a long time and no satisfactory answer has yet been given. This book, which derives from an EEC workshop organized in the frame of the Concerted Action on "PET Investigation of Cellular Regeneration and Degeneration", held in Lyon in February 1992, gives the present state of knowledge in this field based on the most recent studies. Contributions from 24 leading European and American scientists are presented and discussed.

This issue of the Obstetrics and Gynecology Clinics covers the Medical and Surgical Management of Common Fertility Issues. Articles include Infertility Evaluation, Ovulation Induction, and problems such as fibroids, endometriosis, and ectopic pregnancy.