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Books > Science & Mathematics > Science: general issues > Scientific standards
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Acoustic Levitation-Based Trace-Level Biosensing - Design of Detection Systems and Applications to Real Samples (Paperback, 1st ed. 2021)
Loot Price: R5,292
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Acoustic Levitation-Based Trace-Level Biosensing - Design of Detection Systems and Applications to Real Samples (Paperback, 1st ed. 2021)
Series: Springer Theses
Expected to ship within 10 - 15 working days
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This book shows the availability and potential of the coupled
acoustic-gravitational (CAG) field for trace-level biosensing. The
proposed detection scheme also allows the evaluation of the
kinetics and thermodynamics of the reaction occurring on a single
microparticle (MP). This method has wide applicability in important
fields, involving not only chemistry but also life, environmental,
and medical sciences. The author proposes novel trace-level
biosensing based on measurements of the levitation coordinate shift
of an MP in the CAG field. The levitation coordinate of the MP in
the CAG field is determined by its density and compressibility. The
levitation coordinate shift is induced by the binding of gold
nanoparticles (AuNPs) to the MP through interparticle reactions.
Therefore, the quantity of molecules involved in the reaction can
be determined from the levitation coordinate shift. The author
demonstrates the zmol level detection for biotin, DNA/RNA, and
organic molecules. In addition, the kinetics and thermodynamics are
evaluated for various reactions occurring between the MP and AuNP,
such as the avidin-biotin reaction, direct hybridization, sandwich
hybridization, and aptamer-target complexation. This book provides
a new concept based on the CAG field, in which the extent of a
reaction is converted into the levitation coordinate shift, that
is, "length." The proposed method has many advantages over other
methods, e.g., high biocompatibility, high applicability, and short
analysis time. In addition, because the apparatus used in this
study is inexpensive and easy to miniaturize, this method is useful
in important practical fields, such as forensic and environmental
science and diagnosis. Thus, this book inspires many researchers to
apply the present method to their own fields of interest.
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