Legumes are very important plants playing a central role in
biological research. They are a key component of sustainable
agricultural systems because of symbiotic nitrogen fixation and
other beneficial symbiosis with mycorrhizal fungi. Studies on most
of the major leguminous crops are hampered by large genome sizes
and other disadvantages which have hindered the isolation and
characterisation of genes with important roles in legume biology
and agriculture. For this reason Lotus japonicus was chosen as a
model species for legume research some ten years ago. Since then,
many groups around the world have adopted Lotus as a model and have
developed numerous resources and protocols to facilitate basic and
applied research on this species. This handbook represents the
first effort to compile basic descriptions and methods for research
in Lotus, including symbiotic processes, cell and molecular biology
protocols, functional genomics, mutants, gene tagging and genetic
analysis, transformation and reverse genetic analysis, primary and
secondary metabolism, and an exhaustive update of the scientific
literature available on this plant.
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