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Artificial Riboswitches - Methods and Protocols (Hardcover, 2014 ed.)
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Artificial Riboswitches - Methods and Protocols (Hardcover, 2014 ed.)
Series: Methods in Molecular Biology, 1111
Expected to ship within 12 - 17 working days
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Artificial riboswitches and other ligand-responsive gene regulators
make it possible to switch protein synthesis ON or OFF with
arbitrary ligand molecules. Artificial Riboswitches: Methods and
Protocols focuses on the state-of-the-art methods developed in
recent years for creating artificial riboswitches, therefore this
volume could be regarded as a collection of recipes for the gene
circuit elements in synthetic biology and metabolic engineering.
Chapters cover topics such as screening or rational design methods
for obtaining artificial riboswitches that function in either
bacterial or eukaryotic translational systems, protocols for
evaluating the activities of the resultant riboswitches, as well as
protocols for construction of ligand-dependent, trans-acting gene
regulators. Written in the successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols, and notes on
troubleshooting and avoiding known pitfalls. Authoritative and
easily accessible, Artificial Riboswitches: Methods and Protocols
seeks to serve not only bioengineers who aim to reprogram cell
behaviors and molecular biologists who leverage these regulators
for genetic studies, but to all researchers interested in this
fascinating field.
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