Cotton is the most important textile and cash crop and is widely
cultivated in more than 70 countries, including the United States,
China, and India. Because its long life cycle and complicated
genetic background, it is hard to improve cotton using traditional
breeding techniques although it has made much progress in the last
several decades. Currently, transgenic techniques have become a
powerful tool to improve cotton and transgenic cotton is among the
first commercially genetically modified crops. "Transgenic Cotton:
Methods and Protocols" provides a comprehensive collection of
methods for creating and monitoring transgenic cotton and its
application on agricultural and basic research. Divided into five
convenient sections, topics covered include the current status and
perspectives of transgenic cotton, the principle and methods for
making transgenic cotton, the methods for detecting foreign gene
copy and expression in transgenic plants, the improvement of cotton
using transgenic technology, and finally the methods for monitoring
the potential impact of transgenic cotton on environment, including
gene flow. Written in the successful "Methods in Molecular Biology
" series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols, and notes on
troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, "Transgenic Cotton: Methods
and Protocols" will serve as an excellent resource for scientists
as well as graduate students who work on transgenic plants, plant
genetics, molecular biology and agricultural sciences. "
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