"Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC):
Methods and ""Protocols "provides a synopsis of a large array of
different SILAC methods by presenting a set of protocols that have
been established by renowned scientists and their working groups.
These include methods and protocols for the labeling of various
model organisms as well as advanced strategies relying on SILAC,
e.g. for the analysis of protein interactions, the mapping of
posttranslational modifications or the characterization of
subcellular proteomes. Written in the highly successful "Methods in
Molecular Biology "series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by step, readily reproducible laboratory protocols,
and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, "Stable Isotope Labeling by Amino
Acids in Cell Culture (SILAC): ""Methods and Protocols "will serve
students and experienced scientists alike as a valuable reference
of how to make use of the SILAC technology for their own
research.
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