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Protein Sequencing Protocols (Hardcover, 2nd ed. 2003)
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Protein Sequencing Protocols (Hardcover, 2nd ed. 2003)
Series: Methods in Molecular Biology, 211
Expected to ship within 10 - 15 working days
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Determination of the protein sequence is as important today as it
was a half century ago, even though the techniques and purposes
have changed over time. Mass spectrometry has continued its recent
rapid development to find notable application in the
characterization of small amounts of protein, for example, in the
field of proteomics. The "traditional" chemical N-terminal
sequencing is still of great value in quality assurance of the
increasing number of biopharmaceuticals that are to be found in the
clinic, checking processing events of recombinant proteins, and so
on. It is joined in the armory of me- ods of protein analysis by
such techniques as C-terminal sequencing and amino acid analysis.
These methods are continually developing. The first edition of
Protein Sequencing Protocols was a "snapshot" of methods in use in
protein biochemistry laboratories at the time, and this, the second
edition, is likewise. Methods have evolved in the intervening
period, and the content of this book has similarly changed, the
content of some chapters having been superceded and replaced by
other approaches. Thus, in this edition, there is inclusion of
approaches to validation of methods for quality assurance work,
reflecting the current importance of biopharmaceuticals, and also a
guide to further analysis of protein sequence information,
acknowledging the importance of bioinformatics.
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