This detailed book gathers a broad collection of experimental
approaches to assist researchers in setting up different methods to
investigate protein conformational disorders. Beginning with a
section on assays focusing on biophysical approaches to study
protein (mis)folding, the volume continues with sections on
cellular and proteostasis assays as well as assays for protein
folding correction and recovery, combining methods such as thermal
shift assays, in silico improvement of protein solubility, and
compound screening, an important area of research as it may open
avenues for therapeutic strategies. Written for the highly
successful Methods in Molecular Biology series, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips for troubleshooting and avoiding
known pitfalls. Authoritative and practical, Protein Misfolding
Diseases: Methods and Protocols serves as an ideal guide for
researchers seeking to advance our knowledge of protein
conformational disorders.
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