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Ion-Selective Microelectrodes - Principles, Design and Application (Paperback)
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Ion-Selective Microelectrodes - Principles, Design and Application (Paperback)
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The microelectrode technique is today the most widely used method
in electrophy- siology. Microelectrodes offer a unique approach to
measurements of electrical pa- rameters and ion activities of
single cells. Several important breakthroughs in trans- port
physiology have arisen from microelectrode studies. Undoubtedly,
there is a progressively wide-spread use of conventional and
ion-selective microelectrodes. Due to their particular dimension
and properties micro electrodes are exclusive- ly applied to
measurements on living matter. This must have many consequences to
my thoughts on experiments with microelectrodes. In this book, my
concern is fo- cussing on the description of an intracellular
method that should lead to reliable in- formation on cellular
parameters. The methodical basis for any meaningful applica- tion
is treated extensively. However, technical perfection and accurate
results are not the only concern when working on animals and human
beings. Rather, my thoughts are governed by the intellectual and
moral mastery of the experimental ap- proach on living subjects. A
measurement with microelectrodes usually necessitates the sacrifice
of an ani- mal. This is an immense fact, and means that the
knowledge gained by the experi- ment must justify the death of a
living subject.
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